This study was performed in order to determine optimum flushing solution using the direct embryo collection (DEC). Donors, at random stages of the estrous cycle, received a CIDR. 7 days later, 200 mg FSH was treated with 40, 30, 20, 10 mg FSH levels in declining doses twice daily by intramuscular injection for 4 days. On the 3 day administration of FSH, 25 mg was administered and CIDR was withdrawn. After FSH injections were complete, donors were artificially inseminated twice at 12 hr intervals. The donor cattle received 250 GnRH at time of 1 insemination and embryos were recovered 8 days after the 1 insemination. Embryo collection from superovulated donors were performed to flushing by DEC and conventional method. As a results, the average number of recovered embryos were significantly higher as 19.11.40 with DEC method than 12.00.44 with conventional embryo collection method, respectively (p<0.05). Also, The average number of transferable embryos were significantly higher (p<0.05) as 15.81.72 with DEC method than 6.90.35 from conventional embryo recovery procedures. Meanwhile, number of recovered embryos and number of recovered transferable embryos following the number of flushing times until 6 flushing were significantly higher as 8.60.53 and 8.60.53 from 2 flushing time than other groups (p<0.05). No. of Ear. B stage embryos were significantly higher as 3.90.90 and 3.90.90 with 2 flushing time in total collected embryos and transferable embryos (p<0.05). Com M stage embryos were significantly higher as 3.71.00 in 2 flushing time and as 2.20.76 in 3 flushing time for recovered embryos (p<0.05). In transferable embryos, Com. M stage embryos were significantly higher (p<0.05) as 3.71.00 in 2 flushing time and as 2.20.76 in 34 flushing time, also. No. of degradation embryos was significantly higher as 2.20.72 in 5 flushing time, On the other hand, degradation embryos was not observed in transferable embryos (p<0.05). In conclusion, these results suggest that DEC method should effective methods for production of in vivo embryos using less flushing solution following perform until 4 flushing time than conventional embryo collecting method. Also, it might be effectively collection of transferable embryos following more less procedure times compared to conventional embryo recovery methods.

This study was performed in order to simplify the operation and minimize stress of donor and be readily available in the field with low cost and high quality embryos using the Direct Embryo Collection (DEC). Donors, at random stages of the estrous cycle, received a CIDR. 7 days later, 200 mg FSH was treated with 40, 30, 20, 10 mg FSH levels in declining doses twice daily by intramuscular injection for 4 days. On the 3rd day administration of FSH, 25 mg was administered and CIDR was withdrawn. After FSH injections were complete, donors were artificially inseminated twice at 12 hr intervals. The donor cattle received 250 GnRH at time of 1 st insemination and embryos were recovered 8 days after the 1st insemination. Embryo collection from superovulated donors was performed to flushing by non-surgical methods of 3-way, 2-way and DEC (l-way). The average number of recovered embryos were 11.250.63, 12.50.65 and 11.750.48 from operations of 3-way, 2-way and DEC methods, respectively. There were no significant differences among the embryo collection methods. Also, The average number of transferable embryos were 6.250.48, 7.250.48 and 7.250.63 from each embryo collection procedures. The number of transferable embryos was no differences among the 3-way, 2-way and DEC methods, respectively. Meanwhile, the ratio of transferable embryos for all recovered embryos from DEC methods was higher as 61.7 % than 55.6 %, 58 % from methods of 3-way, 2-way. And the flushing solution required for recovering embryos by DEC method was significantly lower as 0.280.32 1 than 1.80.12 1, 1.750.10 1 from 3-way, 2-way methods (p<0.05). Also, the time required for recovering embryos by DEC methods was significantly lower as 272 min than 513, 452 min, respectively (p<0.05). In conclusion, these results suggest that DEC method for embryo collection may be effectively used for production of in vivo embryos using less flushing solution and, it might be effectively available in the field compared to conventional embryo recovery methods using 3-way or 2-way balloon catheter.

The objective of this study was carried out to evaluate the efficiency of sperm collection methods on the post-thaw viability of cat semen. The cat semen was collected by artificial virginal (AV) and electronic ejaculate (EE) methods. The composition of semen extender was consisted of Tris-buffer supplemented with 20% egg yolk and 1% P/S antibiotics in Ext I, and more added 8% glycerol, 1.0% Equex STM paste of total volume in Ext II. The collected semen was adjusted the concentration and then diluted in Ext I for optimal concentration. The diluted semen was cooling to 5℃ temperature in refrigerator for at least 2 hrs and then diluted stepwise with Ext II for at least 1 hrs. After an equilibration for 1 hrs, the cooled semen was packaged in 0.5 ml straw and then freezing on the LN2 vapor over 5 cm above from LN2 and then immersed directly in LN2 for cryopreservation. The frozen semen was thawed in 38℃ water for 15 sec and then evaluated the motility, viability, and morphology. Post-thaw semen were calculated the motility by SMI (sperm motility index). The live-dead sperm was evaluated by Eosin-B and morphological evaluation was by Diff-quik kit staining. The post-thaw concentration (89×106 /ml vs. 128×106 /ml), viability (22.6±10.6% vs. 37.1±26.1%), morphological normality (27.0±50.2% vs. 45.6±123.0%) of EE and AV groups were not significant different, but the post-thaw motility was significant lower in EE than that in AV group (53.1±3.6 vs. 73.6±5.7) (p<0.05). In conclusion, semen collection methods did not significant different between EE and AV groups except of post-thaw motility and so both semen collection methods could be applied in feline semen collection methods.

For a good design, it is important to understand consumers and trend of textile and fashion design. Previous researches on textile pattern design with CAD have been done. They have studied on Korean traditional floral motives/hand painting or some geometric patterns. Few studies have been done on conversational design. Therefore the purpose of study was to observe types of textile pattern design for the recent fiver years and determine fabric pattern trends, especially on the conversational motifs. The types of patterns were analysed with professional panels from the 2005～2009 collection sources, using SPSS program. This study was also develop textile patterns creating using CAD. We conducted design process on motif creating, repeat, and mapping jobs, using textile special design program Prima vision and Photoshop CS3.

정보통신기술의 발달로 가상세계는 현실세계와 더욱 흡사해지고 있고, 가상세계에 대한 활용도 늘어나고 있다. 가상세계 서비스 이용자는 수많은 이용자들로 이루어진 가상 사회(virtual society)의 일원으로서 현실에서 할 수 있는 거의 모든 종류의 활동을 상당한 기간 지속적으로 행하면서 자신의 의사와 상관없이 매우 다양하고 방대한 개인정보를 노출하므로, 가상세계 서비스 제공자는 종래의 온라인 서비스 제공자에 비하여 이용자에 관하여 훨씬 다양하고 방대한 정보를 얻을 수 있고 이를 적절한 도구로 분석하여 훨씬 구체적이고 정확한 이용자 프로파일(user profile)을 구축할 수 있다. 이와 같은 가상세계의 특징으로 인하여, 가상세계 서비스 제공자의 개인정보 수집, 프로파일의 구축, 이용 및 이전 등이 개인정보에 관한 중대한 문제를 야기할 위험성은 매우 높다. 위와 같은 위험을 방지하기 위하여, 가상세계 서비스 제공자의 개인정보 수집, 프로파일의 구축, 이용 및 이전에 대한 적절한 법적인 규제가 필요하고, 이와 관련하여 약관의 규제에 관한 법률 제6조의 불공정 약관 조항에 대한 해석론의 재검토와 정보통신망이용촉진 및 정보보호 등에 관한 법률 중 관련 조항에 대한 개정이 요구된다.

본 논문에서는 수명이 다한 제품이나 소비지-가 더 이상 사용하지 않는 폐기품을 다시 재사용하거나 폐기하는데 필요한 일련의 활동을 위한 역방향 로지스틱스에서의 수거망 설계 문제를 다루고 있다 수거망 설계 문제는 수거지점의 위치와 수요지의 폐기품을 수거 지점에 할당하는 것을 결정하는 문제로 정의할 수 있으며 수거지점은 재활용품이나 폐기품이 위치한 지접 근처에 위치하고 주어진 잠재적인 위치들 중에서 결정하게 된다. 여기서，각 수거지점은 용량제약이 있어 수거

This study aimed to analyze the expression of Hybrid in Contemporary Hair Design that is one kind of total fashion. In this research, by background of the hybrid characteristics of the messes culture art, the hybrid characteristics of the hair design has been defined as characters which is the mixture of timeless spaces, styles, sexes, unsuitable elements and cultures. The results of analysis by the expression of the hybrid hair design after 2000 is like this. As the researching, the hybrid characteristics of the hair design by the mixture of unsuitable elements and styles have been presented the highest. The result of the frequency of the apparition in the hair design molding elements according to the expression of the hybrid hair design, the hybrid characteristics of the unsuitable elements and style has been presented ideological form and the artificial form was the highest. The case of the hybrid of the activated texture, the unsuitable elements, style and timeless space has been presented excellently one after another. In the color tone, the mixture of the style, the unsuitable elements and timeless space has been presented one by one. The case of the hybrid of the image, the unsuitable elements that is the highest the frequency of the apparition was presented the image of the avant-garde, the fantastic and humor one after another.

본 실험은 볏짚 수거시기에 따른 물리적 특성, 사료가치, 소화율 및 기호성에 미치는 영향을 구명하기 위하여 볏짚 수거시기를 9처리 3반복(T1: 벼 수확 후 10일, T2: 벼 수확 후 25일, T3: 벼 수확 후 40일, T4: 벼 수확 후 51일, T5: 벼 수확 후 70일, T6: 벼 수확 후 85일, T7: 벼 수확 후 100일, T8: 벼 수확 후 115일, T9: 벼 수확 후 130일)으로 하여 2007년 10월부터 2008년 2월까지 경북대

Most world-leading companies are aware that Environment and Health and Safety Issues are critical to the product quality and sustainable growth of their company. Environment-friendly efforts are seen in almost all aspects of business operations in an adva

Prostaglandin () can facilitate release of epinephrine from the adrenal gland. The objective was to extend these findings and determine the effects of on sexual activity and semen collection training in sexually inexperienced boars. Boars (n=32; days of age) were moved individually once weekly to a semen collection room equipped with an artificial sow. Before entering the semen collection room, boar received i.m. treatments of at doses of 5 (n=8), 10 (n=8), or 20 (n=8), and control boar (n=8) were not treated. Reaction time (elapsed time after entering collection pen until the start of mounting) for boars receiving 5mg (), 10mg () was shorter (p<0.05) than for controls (). Duration of ejaculation (min) per session was longer (p<0.05) for (10 mg, 20 mg)-treated boars (, ), compared to control (). The number of training session per boars was less (p=0.056) for 10mg-treated boars (), compared to control (). Semen characteristic such as volume, concentration, the number of total ejaculated sperm, were similar for -treated and controls. There was no apparent difference on sperm movement characteristics (Mot: motility, VCL : curve linear velocity, VSL : straight line velocity, VAP : average path velocity, LIN : linearity) after semen preservation by collected with or without treatment. In summary, administration of in boars increased the sexual activity and facilitated the training boars to mount an artificial sow for semen collection, but did not affect semen characteristic.

A movie is a fiction made on a basis of an author's and a writer's imagination, but all sorts of properties mixed with each other and most realistically expresses the era which becomes the background of a movie and acts as a carrier that connects designers with consumers. Thus, this study was carried out to review how the fashion products that designer's intention and commercial value added are expressed in collections by comparing and analysing the costumes in the movie "The Great Gatsby" that described the life of America's upper-class in 1920s and the 04 S/S Y&Kei collection which were proceeding after getting inspiration from this movie. For this, literature materials were inspected in order to make a theoretical review on social and cultural background and costumes history background in 1920s and the photo materials on movie costume were collected and analysed using DVD video captures, as well as the photo materials on 04 S/S Y&Kei were collected and analyzed through the institute providing domestic fashion information. The following conclusion was deduced through this study. First, in 1920s which becomes the background of this study, the slim shape of Flapper which looks like a young and boy became an ideal figure condition and the straight silhouette with low waist line and the short skirt that rose to knee was popular. Second, as a result of analysing movie costume by classifying it in silhouette, colors, and materials, straight silhouette of low waistline with a near colored - tone seen in the pastel series, including white, beige, pink, and gray was mainly constituted and the metal colors like silver and gold were used. As a material, chiffon, satin, velvet, flower patterned prints, and beads were used, which represented luxurious life of women in the upper classes. Third, as a result of comparing and analysing, it turned out that there was a similarity. However, in dress collection for a heroine, some dissimilarity differentiated from a movie costumes was found out in that the dresses in collection expressed moderate beauty and modernism and elegant beauty at the same time by matching a variety of materials and using black color.

These study was carried out to investigate the effects of the collection time, culture time and activation of canine oocytes on in vitro maturation rates. The activated oocytes were cultured in 10% FCS+TCM-199 media containing hormonal supplements (10 IU/ml HCG, 10 IU/ml PMSG, 10 ug/ml gonadotropin) at 5% , 95% air, . 1. IVM rate of in vitro cultured cumulus-attached oocytes recovered from ovaries that collected at follicular and luteal stages of the reproductive cycles were 11.4% and 5.7%, respectively. IVM rate of oocytes recovered from ovaries that collected at follicular stages of the reproductive cycles was significantly higher than that of luteal stage (p<0.05). 2. When IVM was carried out at different periods of 40, 48, and 70 hrs, the IVM rates of oocytes matured in vitro were 2.9%, 8.6%, 5.7%, respectively. These results indicate that the IVM time between hrs gives the highest maturation rate for the oocytes matured at the different stages. 3. IVM rate of oocytes matured in vitro for 10 hrs after single and combined activation treatment by ET, IP and CH and Ca+DMAP, CH+DMAP, ET+CH were respectively. This was higher than that in both single and combined stimulated groups compared to control group ().

The objective of this study was to determine the effect of administration of Prostaglandin F2α (PGF2α) on semen collection training and semen characteristics in sexually inexperienced boars. Boars were moved individually to a semen collection pen and were trained to mount dummy sow. During the first and second semen collection secessions, 4 out of 17 boars and 4 out of remaining 13 boars allowed collection of semen. The 9 boars that failed semen collection from first 2 attempts received immediately 15 mg of PGF2α i.m. (intramuscular injection) upon entering the collection pen for semen collection resulted in successful semen collection from all 9 boars. Total numbers of spermatozoa were higher in PGF2α treated boars but there was no significant difference in % motility kinematics characteristics between control and PGF2α treated groups during 72 hr period. Overall, administration of PGF2α in sexually inexperienced boars increased the sex drive and facilitated the mounting activity to the dummy sow for semen collection.