The Main Control Room(MCR) of Korean Next Generation Reactor(KNGR) has faced entirely new operation environment. The Computerized Procedure System(CPS) is completely different from existed Paper-based Procedure(PBP). The CPS which is improved by evaluation in this study is ready to be applied to the ergonomic foundations. In this study, we redesigned displays of the CPS as a Man-Machine Interface System(MMIS) to maximize human performance and to minimize human error and proposed an alternative display designs of the CPS applying principles related with MMIS.

The study of cornified cell envelope has been used to investigate the differentiation factors and to advance oral carcinogenesis, CE of human oral keratinocytes are in wet condition as saliva containing many proteases, growth factors, and many kinds of bacteria, The analysis of CE in Immortalized human oral keratinocyte(IHOK) derived from normal human oral keratinocyte(NHOK) will be used to study the pathogenesis of oral squamous cell carcinoma, The purpose of this study was to analyze the amino acid component derived from CE of cultured NHOK and IHOK, It will be helpful to study the role of transfected E6/E7 gene in forming CE, and to examine the pathogenesis of oral squamous cell carcinoma, After primry culture of NHOK, IHOK were cultured in KBM bullet kit at 370C under 95% C02 incubator, Growth curve according to calcium concentration, cornified cell envelope measurement(CEM), and protein chemistry for amino acid component of CE were done(Mena :f::SD) , respectively. The obtained results were as follows, lHOK showed small areas of stratification, more compact, with irregular border and tightly apposed cells in 1,2 mM Ca++, Cornified cell envelope exhibited an aggregated group of empty space surrounded by the remained cell membrane, During the terminal differentiation in cultured NHOK and IHOK, insoluble cornified cell envelope formation was increased, CEM of NHOK was about 4 folds than that of lHOK under high calcium, Amino acid component of both groups showed Pro/Glu(SPR) , Gln/Glu(lnvolucrin) , and Gly(Loricrin) in descending order, From the aboving results, ít was suggested that when the terminal dífferentiation in cultured NHOK and IHOK, major amino acid component of CE in cultured lHOK was the same to that of cultured NHOK, It was thought that E6 and E7 gene should be involved in preventing the differentiation and proliferation of IHOK from making CE,

In order to obtain novel genes related to the human craniofacial development, molecular cloning and sequencing, and in situ hybridization using craniofacial tissue sections were performed and followed by protein structure simulation. Totally 231 clones were obtained from the subtracted craniofacial tissue cDNA library of human embryo. Random cloning using the non-redundant clones from the craniofacial tissue of human embryo was done and obtained 398 clones from the premade human chondrocyte cDNA library. Their partial sequence data showed that 214 clones of subtracted cDNA library of craniofacial tissue were still non-redundant in Genebank search. And 20 clones among 498 clones of premade chondrocyte cDNA library were known to be undefined genes. Through in situ hybridization screening in the craniofacial tissue sections of 10 weeks old human embryo 36 clones were found to be positive in specific tissues. Depending on the cell types of sirnilar developmental origin, the positive reactions could be divided into five groups. Among the 20 clones of undefined genes from human chondrocyte cDNA library, 7 clones showed characteristic positive reaction in human cartilage tissue by in situ hybridization. From the simulated protein structure, motif analysis and in situ hybridization studies for the 7 undefined clones, Ch89, Ch96, Ch129, Ch285 clones may function in the outer space of the cell constituting a part of matrix protein complex, and Ch276 as a transmembrane protein which might partic ipate in matrix calcification around chondrocytes. Ch153 is a kind of antirnicrobial protein also acting as an inflammation mediator, and Ch334 clone is a zinc finger protein, of which expression increases in human adult tissues We presume these novel genes from human chondrocytes may provide a new path of chondrocyte development and functions of human craniofacial tissues

This study was conducted to test the anticancer effect of photodynamic therapy using chlorophyll derivative (9-HpbD-a) and 632nm diode laser. Human SNU 1041 cells were seeded into 96 well plate of 104cells/well and cultured for 24 hours. Cells were washed with media containing various concentration of 9-HpbD-a ranging from Oug/ml to 3.75ug/ml. Then 932 nm diode laser was given at various lasering time setting, and at various starting time after ini tial 24 hours of culture. The treated cells were incubated 48 hours and tetrazolium-based colorimetric(M'IT) assay was done to measure the viability of cells For in vivo study, SNU- 1041 cells were xenografted into the back of nude mouse. When the xenografted tumors grew up to 400-600 mm3, the animals were randomly placed into 4 groups: Group 1 (n=20) , PDT group, interstitial injection of 9-HpbD- a (47 ug/kg) followed by irradiation with 3.2 J/c야 of light 6 hours after then i띠 ection; Group II (n=lO) , irradiation with 3.2 J/crrf of light using diode laser; Group III (n=lO), in terstitial injection of 9-HpbD- a only(47 ug/kg); Group IV (n=lO), normal control group. The viability of cells was de creased with increasing lasering time No significant difference of cell viability was noted by variously delayed starting time of lasering. PDT effects were observed in the xenografted nude mouse model Group IV (no 9-HpbD-a, no laser irradiation) was a control group which showed a continuous tumor growth. Group III (9-HpbD-a i띠 ection only) showed no response, Group II (laser irradiation only) sho￦ed 1 complete remission out of 10 (10%) , Group 1 (9-HpbD-a and laser irradiation) showed 13 cpmplete remission out of 20 (65%) , Group 1 showed significant remission rate, comparing to other groups (p<0.05). This study demonstrated anticancer effect of photodynamic therapy using 9-HpbD-a and 632nm diode laser on human squamous cell carcinoma cell line.

Treponema maltophilum, a Group IV oral spirochete, is associated with periodontitis and endodontic infections. In this study we analyzed the functional role of the major surface protein of this organism (MspA) in human gingival fibroblasts (HGFs). The full-length gene encoding MspA was cloned and expressed in Escherichia coli by using the expression vector pQE-30. The recombinant protein (rMspA) was purified by affinity chromatography with nickel-nitrilotriacetic acid agarose and possible contamination of E. coli endotoxin in rMspA was removed by using polymyxin B-agarose. rMspA significantly induced the expression of pro inflammatory cytokines like IL-6 and IL-8 and intercellular adhesion molecule (ICAM)-1 in HGFs, when analyzed by reverse transcription-PCR, flow cytometry, and enzyme-linked immunosorbent assay. Our results indicate that MspA of T. maltophilum may play an important role in amplifying the local immune response by upregulating the expression of proinflammatory cytokines and ICAM-1.

약용식물내 에스트로겐성과 항-에스트로겐성을 조사하고 항암인자를 발견하기 위하여, 본연구는 에탄올추출로 제조된 9종류의 한국산 약용식물에 대하여 재조합효모와 MCF-7 사람유방암세포주를 이용하여 스크리닝하고 비교하였다. 재조합효모를 이용한 실험결과, 7종류의 약용식물에서 에스트로겐성이 나타났고, 4종류에서 안드로겐성이 나타났다. 또한 MCF-7 사람유방암세포주를 이용한 실험결과, 8종류의 추출물이 MCF-7 세포의 성장을 억제하는 것으로 확인되었으며 비스페놀 A와 동시 처치한 경우에도 유의적으로 억제하는 것으로 나타났다. 또한 Clyeyrrhiza uralensis, Cassia tora, Syringa velutina, Zingiber officinale, Malva verticillata, Panax ginseng C.A. Meyer는 식물성 에스트로겐으로서 에스트로겐에 양성인 사람유방암세포의 증식을 유의적으로 억제시티는 흥미로운 결과가 제시되었다. 따라서 이번 연구는 한국산 약용식물이 식물성 에스트로겐과 항암인자로서 이용될 수 있으며, 에스트로겐의 활성을 조사하는데 유용하게 이용될 수 있을 것으로 사료된다.

This paper reports several emotion detecting methods based on various attributes of human voice. These methods have been developed at our Engineering Systems Laboratory. It is noted that, in all of the proposed methods, only prosodic information in voice is used for emotion recognition and semantic information in voice is not used. Different types of neural networks(NNs) are used for detection depending on the type of voice parameters. Earlier approaches separately used linear prediction coefficients(LPCs) and time series data of pitch but they were combined in later studies. The proposed methods are explained first and then evaluation experiments of individual methods and their performances in emotion detection are presented and compared.

Oral cavity offers a good environment for the bacterial growth. 까le species diversity of oral bacteria has been studied for many years based on the classification of liable bacteria. In order to acquire more comprehensive insight into the baα.erial community of human oral cavity, we used molecular ecological methods to clone 16S rDNAs from human saliva. DNA was direαly extracted from saliva collection of human according to age. By 27F, 1492R primer for 16s rDNA’s amplification were enforced. Cloning was achieved from the amplified DNA using pGEM-T Easy Veαor and competent cellJM 109. 159 different base sequences were obtained from saliva samples of four different persons, chosen according to the age group. In all samples, Streptococcus sp. were the most abundant rnicrobes, followed by Prevotella sp., except in the saliva of an old person where Rothia sp. presented the second dominant group 까lis saliva sample showed another and more irnportant characteristics that there were increased species diversity, from pathogenic bacteria like Haemophilus sp. and lautropia sp. to the higher pr'φ。rtion of unculturable bacteria. The same tendency, but less clearly, was found for the saliva of adult smoker in the forties. These results suggest that environmental factors in the oral caviψ of smoker and older person makes favourable condition for the infectious bacteria.

The puφose of this study was to evaluate the role of EGF(Epidermal Growth Factor), EGFR(Epidermal Growth Factor Receptor), aFGF(acidic Fibroblast Growth Factor or FGF-1), bFGF(basic Fibroblast Growth Factor or FGF-2), FGFR(Fibroblast Growth Faαor Receptor), and VEGF(Vascular Endothelial Growth Factor) in the development of the human ameloblastomas For this study 9 subjects, diagnosed as ameloblastomas referred to the Dept. of Oral Path. College of Dentistry, Kyung Hee University, 2 subjeαs of normal oral mucosa with any inflammatory changes were used as experimental, control groups respectively. All the tissues ; experimental and control group were fixed in 10% neutral formalin solution and embedded in paraffm, serial tissue section were made 하1m in thickness and processed in the standard way for immunohistochemical method, using primary and secondary antibodies, for EGF(Antirabbit Ig G, rabbit kit at }:1oo dilution), EGFR(Antimouse Ig G, mouse kit at 1:100 dilution), aFGF(Antirabbit Ig G, rabbit kit at 1:100 dilution), bFGF(Antirabbit Ig G, rabbit kit at 1:100 dilution), FGFR(Antimouse Ig G, mouse kit at 1:100 dilution) , and VEGF(Antirabbit Ig G, rabbit kit at 1:100 dilution), all BioGenex U.S. A. made, followed by the Streptavidin - Horse Radish Peroxidase(InnoGenex, Human-avidin kit) appli때on ， counter stained with Mayer’s hematoxylin stain method. And examined under the biologic microscope, graded -(no epithelial stain), +(weak or focal epithelial stain), ++(moderate or focal intensive epithelial stain), +++(intense generalized epithelial staining) for the epithelial, and connective 따sue component in ameloblastomas and in normal mucosal epithelium on each. Attained results as follows ; 1. EGF, EGFR, aFGF, bFGF, FGFR, and VEGF showed more intense stainability on experimental group compare t，。 that on the control group. 2. EGF, EGFR, aFGF, bFGF, FGFR, and VEGF showed more intense stainability in epithelial component and more intensely stained on the peripheral ce11s of the ameloblastomas. 3. EGF, EGFR, aFGF, bFGF, FGFR, and VEGF showed positive stainability on the stromal tissues but its level is lower compare to that on the epithelial components. Those results suggested that those growth factors take a role in development and progression on the amelob비las와tomas

As long as convicted prisoner is a citizen of the nation, his/her fundamental human rights must be guaranteed to the maximum extent in accordance with the constitutional spirit within the range of not becoming a barrier to accomplish a legitimate penological purpose. When we put too much emphasis on protection of human rights of prisoners insomuch as to disturb and violate the correctional order and regulations, however, it would not only cause life and physical safety of both prisoner and correctional officer to be threatened but to make eligible prisoners unable to return to society through correctional and reformative activities, which are basic and valid penological objectives, and also correctional institute unable to detain an offender in custody that is a premise of imprisonment. Therefore, it is required to accomplish the ultimate penological goals so much as to make convict return to society, while the state should guarantee human rights of prisoners, and, at the same time, firmly establish correctional orders. But there are contradictory relationships between protection of convict rights and establishment of correctional orders, so that problems have risen in due course from the perspective of how to achieve harmonization between the two. Then, it would not be too much to say that to maintain discipline and order in correctional institution up to an appropriate level is the most basic premise for penological practices. In this sense, it can be said that to establish correctional orders in a firm manner is essential for penological practices. If discipline and order is exceedingly strict in observance, however, convict will merely become an object to be controlled, thus that it would be not only difficult to achieve certain expected effects of correctional goals but to bring about worries to infringe human rights because of unwanted limitation of fundamental rights of individuals. So then, it is thought that to set up balanced relations between human rights protection and establishment of correctional order by harmonizing tensive relationships in between is an important task in contemporary correctional practices. In any case, limitation of fundamental human rights of convict, however, should not cross boundary that has been set in order to maintain an orderly prison life having secured physical custody of convict.

지금까지 수년 동안 선박숭무원의 피로는 해양사고의 잠재적인 원인(potential cause) 또는, 인간과실(human error)에 기여하는 것으로써 그 개념이 무시되거나 고려되지 알았다. 그러나 최근 해양사고 자료나 조사에 의하면 피로가 임무수행에 밀접하게 영향을 미쳐서 인간과실을 유발하게 하고 결국 각종 해양사고가 발생한다는 사실을 밝혀내게 되었고, 여기에 대한 많은 관심과 연구가 집중되고 있다. 본 연구에서는 선박승무원의 피로에 대한 개념을 정립하고, 업무수행능력에 영향을 미치는 피로유발요인에 관한 설문조사를 실시하고 그 결과를 분석하였다.

The Study lay Emphasised on to Investigate Human Related Causes of a Pointed End Equipment Accident and the Basic data for Analyzing Human-Error Prevention Program. Peter Son's Model of Human-Error Accident Causation and Cooper's Model of Safety Culture Were Applied to Analyze the Severe Cause of a Pointed End Equipment for Last 5 Years. Through to Analyzing the Cause of Equipment Accident of Human-Error, Expert's Opinion and Experience Theory Method was Reflected. The Analyses Showed What the Immature and Inexperient Error Were Major Causes of a Pointed and Equipment Accident. The Cause of Human-Error was Found with Respect to Human, Tasks, Acknowledge, Organization.

The Purpose of this study was to investigate how an approach to qualitative analysis models of human movement from many of the subdisciplines of physical education : biomechanics, motor development, motor learning, pedagogy. the resuls of the study were a

본 연구의 목적은 각각의 색채 자극 하에서의 생리신호를 측정하고자 하였다. 실험에 사용된 색채는 빨강, 노랑, 녹색, 파랑, 보라, 그리고 백색을 사용하였다. 실험은 방음 암실에서 이루어졌으며, 건강한 성인 남, 여 30명을 대상으로 하였다. 생리신호의 측정은 뇌파와 심전도를 측정하였다. 뇌파의 실험결과는 차가운 색 계통의 색채에서 α파의 상대 전력비가 증가한 것을 볼 수 있었다. 또한 심전도의 HRV 분석 결과도 차가운 색 계통에서 HF/LF의 비가 증가한 것을 볼 수 있었으며, 이 결과로서 색채 조명이 인체의 감성변화에 영향을 미치는 것을 알 수 있었으며, 이 결과를 활용하여 색채 환경 설계에 도움을 줄 수 있으리라 사료된다.