A total of 92 unfertilized human oocytes were treated with ethanol (EtOH), calcium ionophore A23187 (CI) or electric pulse (EP) for activating pronuclear formation and subsequent development. In Experiment 1, there was a significant (P=0.0001) treatment effect on the activation of unfertilized oocytes. No spontaneous activation was occurred in the control, but activation treatments induced PN formation with various efficacy. More unfertilized oocytes (UFOs) were activated after EtOH or EP treatment than after CI treatment. EP was as effective (63.6 %) as EtOH, but fragmentation was observed in 43% of UFOs activated by EP. Proportion of UFOs that formed presumptive haploid PN (2 PNs+1 PB or 1 PN +2 PBs) was 33.3, 0 and 28.6% after EtOH, CI and EP treatments, respectively. In Experiment 2, a significant (P=0.0362) effect of immature oocytes (IOs) status on activation was fecund. IOs at the GVBD-MI oocytes had higher potential to form PN than those at the GV stage or with abnormal morphology (25 vs. 77.8%). The results of this study clearly demonstrated that the treatment of 10% ethanol for 5 min effectively induced the activation of UFOs. IOs could form pronucleus with high efficacy by ethanol treatment, as long as they grew beyond the GVBD stage.

병해충을 막기 위해 농업용 살충제가 광범위하게 사용되고 있다. 농약사용에 따른 생물학적 위해가 우려되며 농약이 또 다른 환경유해요인과 인체에 상승적으로 작용할 경우 농업재해로 이어질 가능성이 있다. 다양한 인자에 의한 DNA손상을 감지하는데 유용한 단세포 겔 전기영동법을 이용하여 살충제와 방사선에 의한 사람 림프구 DNA손상을 평가하였다. 각기 다른 농도로 살충제를 10분간 전처리한 림프구와 정상 림프구에 0-2.0 Gy의 방사선으로 조사한 다음 DNA

Many publications of pointology have been conflicted to define fixed positions of acu-points. These underlying cause results from the historical fact that most of acu-points and meridian systems began at anonymous experiences of many generations. However, if we would believe that the acupoints and meridian systems exit clearly in all of the organism, there were exit the fixed position of acupoints. In this study, we performed bibliographically the investigation fixing the position of CV16. From the investigation, although supported that it exit 1.6 chon (寸) below CV17 in human being, we suggest that CV16 exit below the sternum met with the 7th rib, or the joined point between the sternum and the xiphoid process.

This experiment was conducted to investigate the effect of the cysteine and glutathione on the motility index and morphology of human spermatozoa at the sperm processing in vitro. After treating the sperm with medium containing cysteine and glutathione, we measured the motility index and morphology at 0.5 h and 24 h. 1. Following the sperm culture for 0.5 h after treating the sperm with the medium containing 0, 1, 5, 10 mM cysteine, curvilinear velocity (VCL) was significantly (p<0.05) higher in control than that in all treatments. And straight-line velocity (VSL) was high at 1 mM and average path velocity (VAP) was low at 5 mM and 10 mM. But the motility (MOT) and morphology (NOM) were not different between control and all treatments. Following the sperm culture for 24 h, the MOT was significantly high in treatment groups (58.9, 74.4 and 62.3%), compared with that in control(28.7%) and the VCL was also high in treatment groups (31.4, 37.9, and 34.0 /s), compared with that in control (21.3 /s). The VSL (18.4, 21.7, and 18.9 /s) was significantly higher than control (10.7 /s) and the VAP (20.3, 24.7, and 21.4 /s) in treatments was also compared with that in control (12.6 /s). The NOM was not difference between control and treatments. 2 Following the sperm culture for 0.5 after treating the sperm with the medium containing 0, 1, 5, 10 mM glutathione, the MOT, VCL, VSL, VAP, and NOM were not different between control and treatments. Following the sperm culture for 24 h, the MOT was higher in treatment groups (82.9, 83.6, 83.4%) than in control (51.1%) and the VCL was higher in treatment groups (50.9, 51.3, and 49.4 /s) than control (34.1 /s). The VSL was also higher in treatment (17.1 /s) and the VAP was also higher in treatment groups (30.1, 32.5, and 29.7 /s) than in control (19.8 /s). The NOM was not different between control and treatments.

본 연구는 주거환경에 대한 시각 감성을 대표하는 어휘를 선발하기 위해 수행되었다. 어휘수집의 첫 단계에서는 주거환경 중 시각 환경에 대한 감성을 표현하는 어휘 235개를 수집하였다. 두 번째 단계에서는 수집된 어휘를 다른 피험자들에게 제시하여 주거 환경의 분위기를 나타내는 어휘 로서 적절함의 정도를 7점 척도로 표시하도록 하여 매우 적절하다고 판단된 24개의 어휘를 선발하였다. 세 번째 단계에서는 이들 어휘를 무선 적으로 두 개씩 짝을 지어 두 단어가 유사한 정도를 7점 척도로 평가하도록 하였다. 이 설문으로부터 나온 데이터에 대해 요인분석, 군집분석, 다차원분석을 실시하여 시각적 주거환경에 대한 9개의 감성어휘를 추출하였다. 이와 함께 최종 단계에서 연구자들이 400여장의 실물 사진 열람을 통해 추출된 9개의 감성 어휘가 실제 시각적 주거환경을 나타내는 데에 적함한지를 다시 한번 검증하여 다음과 같은 10개의 어휘를 선발하였다. ‘안락한’, ‘개방적인’, ‘세련된’, ‘경쾌한’, ‘개성적인’, ‘단순한’, ‘화려한’, ‘중후한’, ‘고풍스로운’, ‘전원적인’.