This study was carried out to investigate On in vitro fertilization, survival rate and developmental rate of rapidly frozen bovine immature oocytes. Immature oocytes cultured for 1, 12, 24, 48 hours in 20% FCS + TCM-199 medium and thereafter rapidly freezing-thawed oocytes inseminated with capacitated sperm. The immature oocytes following dehydration by 1.5M DMSO + 2.0M glycerol + 0.25M sucrose + TCM 199 media + 20% FGS were directly plunged into liquid nitrogen and thawes in 3 water. Rapid freezing embryos co-cultured in 20% FCS + TCM-199 media containing hormones(21U/mL PMSG, 21U /mL hGG and 1 g /mL 17-estradiol) and cumulus cells(1 x 105-6 cells). Survival rate was defined as development rate on in vitro culture or FDA-test. The results are summarized as follows ; 1. The in vitro maturation and fertilization rate of immature bovine oocytes on in vitro maturation period(1, 12, 24, 48 hrs) before rapid freezing4hawed were 57.1%, 45.7%, 37.1%, 25.7% and 40.0%, 31.4%, 20.0%, 11.4%, respectively. 2. The survival rate of immature bovine oocytes on in vitro maturation period(1, 12, 24, 48 hrs) before rapid freezing-thawed were 33.3%, 26.7%, 20.0%, and 10.0%, respectively. The survival rate of rapid freezing4hawed immature oocytes was significantly lower than that of non-freezing oocytes. 3. The survival rate of rapid freezing4hawed excellent and good bovine embryos co-cultured in 20% FCS + TCM-199 media containing hormones(PMSG, hCG, 17-estradiol) and cumulus cells 4 to 5 hrs and 20 to 24 hrs were 35.0%, 15.0% and 25.0%, 15.0% and 40.0%, 20.0% and 30.0%, 15.0%, respectively. The survival rate of embryos co-cultured in TCM-199 media containing hormones and cumulus cells was significantly higher than that of non co-culture.

Vibrio vulnificus에서 lipopolysaccharide(LPS)를 추출하여 지방산 조성을 분석한 후 이 결과를 Escherichia coli LPS와 Salmonella typhimurium LPS의 것들과 비교하였다. Vibrio vulnificus LPS의 주 지방산은 myristic acid(C14:0, 41.37%)이었고 Escherichia coli LPS는 lauric acid(C12:0, 37.03%), Salmonella typhimurium LPS는 capric acid(C10:0, 48.60%)로 서로 달랐으나 이 세가지 지방산이 각 LPS의 주성분이었다(70%이상).

dynamic strain gage와 12bit AD(analog to digital converter)를 이용한 새로운 제진특성 측정 장치를 제작하였다. 이 장치를 이용하여 일반재료와 고제진재료의 제진특성을 연구하였다. 또한 열처리 조건, 초기 진동 진폭, 그리고 내부응력의 변화에 따른 SDC(specific damping capacity)변화에 관하여 연구하였다. 일반재료와 제진재료의 비교에서, 제진재료는 진동을 가한 후 0.4초 이내에 진동 진폭이 거의 사라졌지만, 같은 시간에 일반재료의 진동 진폭은 거의 감소하지 않았다. Fe-16wt. %Cr계 합금의 제진 특성은 노냉일 때 SDC max 가 40%이상이었고, Fe-5.5wt.%Ai합금의 제진 특성은 공냉일 때 SDC max값이 30%이상이었다. 초기 진동 진폭이 증가할수록 최대 제진 특성치는 낮은 진동 진폭 영역으로 이동하였다. 제진 특성은 내부 응력이 증가할수록 급격한 감소를 보였으며, 본 연구에서 개발한 제진측정 장치는 낮은 진동 진폭의 영역에서 정확한 제진 특성 측정이 가능하였다.

본 연구는 건설 디지털 제조를 위한 3D 프린팅 기술 개발을 위하여, 플라이애시 및 고로슬래그 미분말 등의 알칼리 활성화 결합재를 Binder Jetting 3D 프린팅 방식으로 출력하고, 후처리 방법을 통해 출력물의 압축강도를 증진하는 것이다. 진공 펌프 및 규산나트륨-수산화나트륨 복합 수용액을 적용하여 후처리 방법을 검토하였으며, 후처리 용액에 침지한 후 -0.1 MPa 진공 압력으로 처리한 공시체의 압축강도는 11.04 MPa 로써 강도 증진을 확인하였다.

This study was performed an evaluation of compressive strength of NSB mortar according to BP contents and curing conditions. As the results of study, compressive strength increase as BP content and curing temperature was increased. When considering the strength properties of BP NSB mortar, the proper BP content and curing temperature are thought to be 20% and 60℃ respectively.

The sliding materials for bridge supports are used to ensure the seismic performance or to absorb thermal deformation of bridge decks. The sliding plates in the bridge-bearings directly transfer vertical loads and horizontal displacements to piers. To construct bridge economically, bridge-bearings are required to endure higher vertical load capacity. Therefore, smaller and stiffer sliding materials have been being developed to improve vertical loading capacity of bearings. In this study, friction coefficient tests were accomplished with the sliding materials applied for bridge bearings. Three different types of friction materials (PTFE, C-Lube, and UHMWPE) were tested with equivalent compressive stress, velocity, and displacement respectively. Based on the experimental results of each material, the horizontal stiffness and energy dissipation capacities of bearings were compared and analyzed through the design of the friction pendulum bearing as well.

It was found that the firming agent was satisfied in terms of mobility for improving compactibility and filling characteristics since reviewing the basic properties of the fluidized filler according to the types of firming agent in domestic market. But, it was difficult to secure adequate strength for re-excavation.

The chloride penetration characteristics of concrete exposed to the marine environment were evaluated by chloride migration coefficient and the surface chloride content, and characteristics of the marine environment such as spray zone and tidal zone are considered

The chloride penetration characteristics of concrete exposed to the marine environment were evaluated by chloride migration coefficient and the surface chloride content, and characteristics of the marine environment such as spray zone and tidal zone are considered. 요

We analyzed the variation in occurrence time and intensity of attacks by the rice stem borer (Chilo suppressalis Walker) on several crops. This study aimed to understand how C. suppressalis damages crops and to utilize this basic data for the establishment of environment-friendly control methods like pheromone traps. This study surveyed the changes in occurrence patterns of C. suppressalis, biological characteristics of overwintering larvae, and the efficacy of different types of pheromone traps the years. We found similar occurrence patterns of C. suppressalis in different crops. In addition, occurrence time of the first generation was advanced. Overwintering larvae showed no difference in pupal period and weight compared to the non-overwintering ones. However, the larval period was reduced to 19.1 days in Miscanthus field, in contrast to that in the paddy fields. It was confirmed that larvae of C. suppressalis generally prefer the lower part of the stems of Miscanthus. Efficiency of the emergence trap was confirmed to be greater than that of the delta trap for capturing C. suppressalis adults. However, it is necessary to adjust the control period because of the advancement in occurring time of C. suppressalis in recent years. The larvae of C. suppressalis experience favorable environmental conditions for overwintering in Miscanthus fields. The major Miscanthus fields are generally located in the areas protected for sources of drinking water, owing to which spraying of chemical pesticides is very limited. The results of this study provide important inputs for the development of environment-friendly control methods.

Bacterial fruit blotch (BFB) is one of most important diseases in Cucurbitaceae due to infection of Acidovorax citrulli, causing huge economic losses damage worldwide. This seedborn disease spread rapidly at period of high temperature and humidity. The eco-friendly farming is getting popular. So far there was no effective agent to control BFB in eco-friendly farming. Therefore, effect of the material based on chinese nut-gall extract with antibacterial activity against BFB to was tested against A. citrulli. Different hosts showed various symptoms of BFB. Liquid formulation among exhibited most effective anti-bacterial activity on A. citrulli. Pot experiment in greenhouse showed the potential as an control agent of BFB in cucurbits. The treatment of material based on chinese nut-gall extract showed the positive effect on survival of the watermelon seedling and on the length of the cucumber seedling treated with A. citrulli. We cautiously conclude that the material based on chinese nut-gall extract used in this study may be good agents against major diseases of cucurbits in the future even though it is require to be tested with more study on field test.

Barley yellow mosaic virus (BaYMV) is transmitted by a root-inhabiting Polymyxa graminis (P. graminis) and thus the disease is called “soil-borne”. In this study, the presence of P. graminis was confirmed by PCR test based on specific sequences of P. graminis type Ⅰ (P. graminis f. sp. temperata) Internal Transcribed Spacer (ITS). P. graminis was detected in the BaYMV infected soil and root of barley plants. The monitoring of P. graminis was conducted in March 2015 in 8 korean provinces. It was detected in the soil of all collected regions. This is the first report on a P. graminis a survey of Korea.

심장혈관 조영술과 심장혈관 중재술은 심장혈관 질환을 치료하는 안전하고 효율적인 시술이다. 그러나 시술이 이루어지는 동안 환자와 시술자의 방사선 피폭에 대해서는 주의가 필요하다. 본 연구의 대상으로는 2015년 9월부터 2016년 1월 사이에 강원도 소재 한 대학병원의 심혈관센터에서 시행된 147명의 심장혈관 조영술(CA)과 심장혈관 중재술(CI)을 시행한 환자를 대상으로 시술자의 피폭선량을 평가하였다. CA만을 시행한 경우 시술자의 유효선량은 최소 0.39 uSv, 최대 30.6 uSv를 나타냈고 평균 유효선량은 4.11 uSv를 나 타냈다. CI를 시행한 경우 유효선량은 최소 1.618 uSv, 최대 202.805 uSv를 나타냈고 평균 유효선량은 34.41 uSv를 나타냈다. 본 연구의 결과에서 갑상선 위치에서 측정된 선량을 머리가 받은 선량으로 대체할 경우 PCI에서 평균 받는 선량을 연평균 1000건의 시술을 시행한다고 할 경우 90 mSv/year 에 해당한다. 이를 2년 누적할 경우 눈의 제한선량인 150 mSv를 초과하는 수치이다. 심장혈관 중재적 시술은 의료 방사선의 피폭 에 있어 CT 다음으로 큰 부분을 차지하고 있다. 따라서 시술이 시행되는 동안 환자와 시술자의 방사선 방 어가 적절히 이루어질 수 있도록 하여야 하며 검사의 최적화와 정당성을 확보하여 안전한 검사가 될 수 있 도록 해야 한다. 이를 위해 시술자에게는 적절한 교육이 이루어져야 하고 시술 중에는 개인방어 장비를 착 용하여야 하며 모니터링을 통해 환자와 시술자의 피폭선량을 줄이는 노력이 필요하다.