This study was performed to analyze a saltiness enhancement at the same salt content through multiple emulsion. We compared the samples with different conditions to determine the optimum stability conditions of water-in-oil through layer separation rate, microscopic observation and size analysis. Four electrolytes such as NaCl, KCl, MgCl2, and CaCl2 were used and agar contents ranged from 0 to 1% were experimented at different volume ratios including 5:5, 4:6, 3:7, 2:8, and 1:9 of water and oil. As a result of this study, the droplet size according to the electrolyte type did not show significant differences (p<0.05). Therefore, KCl was used to facilitate in-body excretion of NaCl in the outer water phase, and corn oil containing 8%(w/w) polyglycerol polyricinoleate was used as oil phase. When the volume ratio of water and oil was 3:7, 2:8, and 1:9, the layer separation rate was relatively slow and droplet size was also small. It reveals that the particle size becomes smaller as the water volume ratio decreases. However, considering the amount of water to be stored and eluted on the inner water, appropriate volume ratio of water and oil should be adopted to 3: 7. At Microscopic observation depending on agar concentrations, small particle size appeared at 0.2% and 0.4% agars. When the water and oil ratio was fixed at 3:7, the particle size was measured at 0.2% and 0.4% agar using a zeta sizer. In conclusion, the droplet size of 0.2% agar was smaller than 0.4%. Therefore, the most stable water-in-oil emulsion was obtained with 0.2% agar, when water to oil ratio was 3:7.

Probiotics are defined as advantageous microorganisms to human when they are ingested. However, without any protection, the viability of microbes and their adhesive ability to surface of colon decreases through acidic condition such as stomach and intestines. Therefore, many studies have been conducted to figure out to enhance not only the viability of probiotics, but also its adhesion for increasing effect of probiotics. In this study, extrusion method was conducted to encapsulate Enterococcus faecium. E. faecium-alginate solution was injected to CaCl2 solution with regular side air injection. To prevent coagulation of beads, stirring was conducted in CaCl2 solution and encapsulated alginate-Ca2+ microspheres were produced. For optimal encapsulation condition, air pressure was 100 mbar, flow rate of E. faecium solution was 0.02 ml/h and stirring rate was 200 rpm. For mucoadhesive ability, Monolayer of HT-29 cells used as a colon cell and encapsulated cells were inoculated and incubated in 37℃, 5% CO2/95% air atmosphere for 1 h. Encapsulation efficiency of the encapsulation method used in this study was 98.2%. For mucoadhesive test, the concentration of inoculated E. faecium was 9.9×108 CFU/ml and the concentration of adhered E. faecium was 1.6×106 CFU/ml. In conclusion, encapsulation efficiency of extrusion method was high enough to be accepted for this study, however, alginate-Ca2+ microspheres revealed lower adhesive ability compared to expectation. Therefore, it needs further studies to increase adhesive ability with other polymers.

Adhesive capsulitis of the shoulder is a common cause of pain that occurs during shoulder movement, thereby restricting shoulder rotation in clinical practice. Although most patients respond to pain relief treatment (NSAID or corticosteroids) by improving their range of motion, it remains poorly understood without any definitive treatment algorithm. In addition to immune cells, synoviocytes, chondrocytes and osteoblasts in the joint are known to produce pro-inflammatory mediators such as reactive oxygen species (ROS), inflammatory cytokines and lipid mediators, presumably contributing to the pathogenesis of osteoarthritis (OA) and adhesive capsulitis. Although inflammation and also fibrosis are proposed to be the basic pathological changes of a frozen shoulder, there is a lack of information regarding the downstream targets of the pro-inflammatory ROS signaling pathway in the synoviocytes and also how these ROS targets are modulated at the transcription level by a corticosteroid - dexamethasone. In this study, we used human fibroblast like synoviocytes (HFLS) to characterize the signaling targets of ROS by employing a human DNA microarray tool and studied the role of dexamethasone in this process. Our data suggest that several genes such as FOS, FOSB and NFkBIZ, which are known to be involved in pro- or anti- inflammation response, are modulated at the transcription level by ROS and dexamethasone.

Salt is generally used for food seasonings and preservations as a common ingredient. However, excess salt intake has generated health issues such as high blood pressure, osteoporosis, stroke, and heart diseases. Recently, desires and interests of low-salt cooking have been increased among people who want healthy diets. The aim of study was to compare the physicochemical characteristics of a fermented squid sauce added with vitamin C and commercial low-salt soy sauces. Thawed and crushed squids were fermented until the solid squid became liquid at 25°C with an addition of 5% (g/g) vitamin C. Then, fermented squid sauce was heated at 100°C for 30 min and filtered. All samples were measured in multiple aspects of amino acid nitrogen, salt, sugar and water content, pH, chromaticity and brown color, and sensory test. In the results, color values showed no significantly difference between all the samples (p>0.05). Water content value of the fermented squid sauce was the highest among samples. Brown color, salt contents and sugar contents of the fermented squid sauce were significantly different than other low salt soy sauces with an exception of the sauce made with functional salt. These results showed a similar tendency as those of sensory evaluation. As conclusion, the possibility of fermented squid sauce added with vitamin C showed a possibility as a candidate of low-salt soy sauce.

In this study, we investigated the change of physical properties associated with the temperature of vegetable mixed oil and fat in order to produce vegetable oil and fat suitable for plant meat production. The canola oil and coconut oil were mixed at various weight ratios, and the phase change temperatures by the ratio of two oils were measured using the differential scanning calorimetry (DSC). Storage modulus (G'), loss modulus (G") and viscosity were measured using a rheometer at 20-40°C and 0.4 Hz-100 Hz. Storage modulus (G') at constant frequency (10 Hz) was measured in a continuous section of 10-50°C. As the coconut oil content increased, the peak of the melting point moved to the lower side. The viscosity was higher in order of canola oil, mixed oil, and coconut oil, and the viscosity showed a tendency to decrease as the temperature increased. In the liquid state, it showed a tendency to increase after the value of storage modulus (G') and loss modulus (G") decreased from 0.634 Hz-1 Hz. The conversion time point of storage modulus (G') of continuous temperature change is consistent with the melting point temperature of DSC, as the passed start at 10°C, storage modulus (G') increased with an exception of canola oil. Using these results, we will pursue to produce a mixed plant oil applicable to the production of vegetable meat.

The hepatitis E virus (HEV) is a leading causative agent of acute hepatitis in humans. Zoonotic HEV strains have been isolated from several animal species, including pigs. New HEV variants have been recently isolated from camels in the Middle East. In the present study, fecal samples from fallow deer, formosan deer, alpaca, and guanaco were analyzed for the detection of HEV. One HEV strain was detected from guanaco, a species of camelids. The nucleotide sequence of guanaco HEV was identical to those of deer HEV-3 strains, which implied the cross-species transmission of HEV-3 from deer to guanaco.

Social interactions have been established as a means to help promotions and sales for manufacturers and retailers. Word-of-mouth (WOM), in particular, is proven to increase awareness and drive purchases. Given that small offline retailers have limited resources in marketing, online WOM can play a key role for their offline business performance. In this paper, we focus on two types of online WOM, public discussion and social referral, and study their generation processes by taking into account the multichannel context of both online purchases and offline consumption. To this end, we combine data from three sources: product (or deal)-level sales from a major deal site in South Korea, social interaction records collected by web crawling, and retail revenues at a district level from Korea National Statistical Office. We use a multivariate poison lognormal model to estimate three equations in the same structure with correlated errors, which only differs by the following dependent variables: number of product discussion, number of social referral, and the number of social coupons sold. Our empirical analyses suggest the following. First, the two types of WOM respond in opposite directions for the influencers in the multichannel sales context: the greater number of co-located online deals decreases public discussion but increases social referral. Next, the larger offline retail size increases public discussion, but has no significant effect on social referral. Finally, the results provide practical insights that small offline retailers can improve sales in the multichannel context by effectively managing the generation of different types of online WOM.

The research shopping involves making use of multiple channels for a single shopping incident, such as searching from one channel and buying from another (Neslin et al. 2006, Neslin and Shankar 2009, Verhoef, Neslin and Vroomen 2007). This is an opportunistic behavior on the side of the consumers, and may result in an unfair advantage of the retailers in one channel consumers choose to purchase from. For instance, consumers may browse a product in a brick-and-mortar store, making use of the retail space and sales assistance, and proceed to buy online from another retailer which offers lower price. This is called a “showrooming” behavior (when focusing on the consumer), or channel-free riding (when focusing on the business) (Mehra, Kumar, & Raju 2013, Van Baal & Dach 2005). Offline retailers are wary of this phenomena that may lead to higher costs and lower sales, and attempt to discourage it (Rapp et al. 2015). For instance, Borders and Circuit City, the former US national chains with a substantial offline market presence, went out of business presumably due to the showrooming phenomenon (Gustin, 2012; Passariello, Kapner, & Mesco 2014). However, recent studies show that research shopping across multiple channels within one company can be managed and contributes to firm profitability in the long run (Verhoef, Kannan, & Inman 2015, Zheng et al. 2016). Kumar & Venkatesan (2005) reveal that consumers who uses multiple channels are the ones with greater customer lifetime value and with less churn intention (Blattenberg, Malthouse, & Neslin 2009, Stone, Hobbs, & Khaleeli 2002). Neslin & Shankar (2009) suggest a practical discussion on market strategy in which customers who visited offline stores can be encouraged to repeat-purchase or to foster brand involvement by maintaining contact in another channel (e.g., email newsletter). It is particularly desirable to lure online consumers to an offline site, since they tend to make greater amount of purchases in a brick-and-mortar store (Ansari, Mela, & Neslin 2008). In the age of effortless access to and switching among a plethora of channels by consumers, it is critical for a business to understand and make best of the situation. In this regard, understanding consumers in terms of who are more likely to display research shopping tendency is crucial. Depending on whether a person tends to do research shopping, business should suggest and offer different channels for different purposes (Verhoef, Kannan, & Inman 2015, Zheng et al. 2016). For instance, those with high tendency to research shop can be approached in one channel, and nudged to another for purchasing. Those with low tendency should be directed to the final purchasing channel. With this in mind, we aim to investigate research shopping behaviors and individual covariates of these shoppers using individual-level responses. We conducted an online survey in France during September 2014 in cooperation with an anonymous global marketing research firm. The survey focused mainly on exploring customers’ shopping behavior in the apparel industry, as customers’ research shopping behaviors are salient in this industry because of its experiential attributes (Girard, Silverblatt, & Korgaonkar, 2002; Klein, 1998), while traditional patterns of purchasing using only one channel is also prevalent. After a discussion with the research firm about the French apparel industry and main customers’ demographics, we restricted the respondent pool to those between the ages of 25 and 54 who have abundant experience and an active role in apparel shopping. Responses from a total of 400 participants were used in the analyses. The dataset includes individual-level shopping characteristics, demographic information, and the extent of their research shopping behavior. Specifically, each customer was asked about their apparel shopping history (purchase frequency and expenditures on apparel) over the last three months for both offline and online retailers. In addition, demographic information such as gender, age, and educational background were asked. Using multiple questions, we captured shopper’s shopping traits, such as deal-proneness, quality-consciousness, and the degree of their shopping budget flexibility. To explore the individual characteristics of research shoppers, we modeled the probability of being a research shopper using a logistic regression model. From our modeling results, we suggest two notable findings. First, customers’ qualityconsciousness significantly increases their research shopping behavior, while their deal-proneness exerts little to no influence. We conclude this is due to extensive and systematic search tendency shared by quality-conscious customers, based on previous findings in the literature. That is, quality-consciousness induces customers to search carefully across multiple channels to check on multiple quality dimensions of the options at hand, and possibly discover other similar options that may maximize their satisfaction (Lysonski, Durvasula, & Zotos 1996, Sprotles & Kendall 1986, Wesley, LeHew, & Woodside 2006). However, deal-proneness is not associated with research shopping behavior presumably because the one dimension that these customers value (i.e., price) can be easily searched in one channel—the online channel. The literature supports this finding, since studies show that deal-prone customers tend to shop more online (Close & Kukar-Kinney 2010, Devaraj, Fan, & Kohli 2002, Zhou, Dai, & Zhang 2007). We also find that the association between quality-consciousness and research shopping behavior is more pronounced when the shoppers are flexible with their shopping budget. That is, when shoppers are both quality-conscious and willing and able to consider other options or additional items beside the one they have originally planned before shopping, their research shopping tendency is enhanced. We explain that this is because consumer behaviors and decisions are made upon limited resources (e.g., time, effort, and money), and that customers with flexible resources are more likely to extend and manage their choice set (Maity, Dass, & Malhotra 2014, Malhotra 1982). These consumers do not refrain from including options that are better yet more expensive, as they can afford the superior option that suits their tastes (Becker, 1965; Ghose & Han, 2011), and without the fear of creating regrets due to discovering unaffordable options (Lenton, Fasolo, & Todd 2008). We expect our work on research shopping to provide insights to both researchers and practitioners, as today’s multi-channel environment provides opportunities for businesses to manage their customers strategically over several channels they are present in. Therefore, the research is expected to be a useful reference for understanding multi-channel shoppers for the academics, and a valuable guide to retail firms that aim to not only cope with the multi-channel environment but to become a true omni-channel player.

선박엔진은 잔사유를 에너지원으로 활용하여 운항할 수 있으며, 이를 활용한 선박에서 환경 규제와 경제성을 모두 만족시키는 다양한 방안들이 모색되고 있다. 그 중에 한 방안으로 연료 첨가제를 활용하는 기술이 있을 수 있다. 분산제와 연소촉진제는 잔사유활용 시 엔진의 연소특성 촉진에 기여할 것이라는 기대를 받고 있다. 따라서, 본 연구에서는 연소성 분석 장비(FIA/FCA)와 열 중량 분석 장비(TGA)를 활용하여 잔사유 연료첨가제가 혼합된 잔사유의 연소성을 분석하였다. 연소성 분석 장비(FIA/FCA)의 결과로는 연소에 의한 일의 총량을 분석하도록 분석법이 개발되었으며, 이 때문에 본 연구를 통하여 동일 장비를 활용하면서도 연소 효율을 간단하게 평가할 수 있는 방안을 제시하였다. 연소성 분석 결과인 ROHR 곡선으로부터, 단순한 삼각함수를 활용하여 연소특성을 예측할 수 있는 방안을 제시하였으며, 이 기법을 활용하여 기존의 압력 곡선과 유사한 결론을 도출할 수 있었다. 열 중량 분석(TGA)의 경우 연료유의 증발 특성에 민감하게 반응함을 확인하였고, 첨가제가 연료유 증발에 효과적으로 작용함을 확인하였다.

The present study was made to assess annual changes on seasonal occurrence of Apiformes, commonly known as bees, as global warming apparently happens. Bees were collected from ten sites using a malaise trap for each site, and sample collection was made every two weeks from April to September in 2011 to 2014. In overall diversity, abundance and richness of bees appeared higher in northern sites, particularly Gwangneung and Hongcheon, than southern. The abundance of bees declined sharply in 2013 and decreased even further in 2014. Among the bee families the most decreased families were Halictidae and Andrenidae. In order to see the effect of annual temperature fluctuation correlation analysis was used with collection data and weather data. However, the result indicated that the decline of the bees was not significantly affected by changes of temperature although most of the bee families except Halictidae showed negative relation with temperature data(p < 0.05). In addition we also analysed the bee collection data and flowering data during spring in Gwangneung. Dufrene-Legendre indicator species analysis (ISA) for latitudinal sites identified seventeen indicator species (p < 0.05) that were limited to northern region.

Macrophages play an important role in both the innate and adaptive immune responses. These include phagocytosis, killing of microorganisms, antigen presentation, and induction of immune cytokines and antimicrobial genes. Macrophage activity is reported to be controlled by diverse exogenous antigenic or endogenous metabolic molecules, and the underlying mechanisms are well documented in human and mouse macrophage cells. Bacterial lipopolysaccharide (LPS) is known to be one of the most potent stimuli activating macrophages through the toll like receptor 4 (TLR4) signaling pathway. There are other antigenic molecules, such as muramyl dipeptide (MDP) and outer membrane protein A (OmpA), that are also known to activate immune cells. On the other hand, short chain fatty acids (SCFAs) such as acetate and butyrate are produced by gut microbiota and control host energy metabolism and signal transduction through GPR receptors. However, there are few studies demonstrating the effects of these molecules in macrophages from domestic animals, including domestic pigs. In this study, we attempted to characterize gene expression regulation in porcine macrophages (PoM2, Pig Monocytes clone 2) following treatment with LPS, MDP, OmpA, and two short chain fatty acids using porcine genome microarray and RT-PCR techniques. A number of novel porcine genes, including anti-microbial peptides and others, appeared to be regulated at the transcriptional level. Our study reports novel biomarkers such as SLC37A2, TMEN184C, and LEAP2 that are involved in the porcine immune response to bacterial antigen LPS and two short chain fatty acids.

Generally, Cerambycidae inhabites in tropical and subtropical zones, and they are large family among Coleoptera and distributed over 20,000 species worldwide. They typically characterized by extremely long antennae, which are often as long as or longer than the beetle's body. Order Cerambycidae are divided into 11 subfamilies in the world, among them, there are 304 species of the 159 genera in 7 subfamilies from Korea up to date. The genus Anoplophora is belonging to the tribe Agniini in the subfamily Lamiinae. Most species of Anoplophora are showed their beautiful colors on the elytra, pronotum, antennae and ventral parts etc. These colors superficially seems like mere patterns of the integument. Usually, colored pubescent patches of the elytra in Anoplophora are arranged in round spots, in others they are arranged in transverse bands. And also, the species of this genus have very long antennae (about 1.3-1.6 times the body length in males, 1.0-1.5 times the body length in females of most species) then others and mostly body size are large, some species is over 50mm. In this paper Anoplophora freyi is newly recorded in Korea. Consequently, a total of three species of the genus Anoplophora are recorded in Korea.

The present study was carried out to revise the species of Chlaeniini in Carabidae from South Korea, using both classic taxonomy and molecular analysis. Another aim is to decide the taxonomic position of Chlaenius micans Fabricius of which subgeneric status in the genus has been in question. As a part of the results, Chlaenius spathulifer Bates, 1873 was newly recognized to Korean insect fauna. With this new addition Korean Chlaeniini contains now in total 34 species and two genera. Molecular analyses using CO1 sequences of representative species for six subgenera in Chlaenius were conducted to determine the position of C. micans. The result of the analyses suggested that C. micans did not belong to the subgenus Achlaenius where C. micans had been doubtfully positioned despite distinct morphological differences from the other four species of the variicornis-group (C. sericimicans, C. variicornis, C. kurosawai and C. ocreatus) in the subgenus. This study provids a key to species, photos of adult habitus, photos of male genitalia and redescriptions of 28 Korean species in Chlaeniini.

Subgenus Achlaenius Manldl, 1992 belonging to the genus Chlaenius Bonelli (1810) of the carabid tribe Chlaeniini. This subgenus was characterized by strong densely pubescent of pronotum and elytra. This subgenus was recorded five species in Korea and Paleoarctic region : Chlaenius micans Fabricius, 1792, C. ocreatus Bates, 1873, C. sericimicans Chaudior, 1876, C. variicornis Morawitz, 1863 and C. kurosawai Kasahara, 1986. However, subgenus Achlaenius was rarely studied in Korea until now because of similar morphological patterns and difficult identification with allied species. As the results, totally four speciesof this subgenus from Korea were reviewed photos of adult and genitalia in male, key to the species of subgenus Achlaenius from Korea

We report a highly sensitive NO2 gas sensor based on multi-layer graphene (MLG) films synthesized by a chemical vapor deposition method on a microheater-embedded flexible substrate. The MLG could detect low-concentration NO2 even at sub-ppm (<200 ppb) levels. It also exhibited a high resistance change of ~6% when it was exposed to 1 ppm NO2 gas at room temperature for 1 min. The exceptionally high sensitivity could be attributed to the large number of NO2 molecule adsorption sites on the MLG due to its a large surface area and various defect-sites, and to the high mobility of carriers transferred between the MLG films and the adsorbed gas molecules. Although desorption of the NO2 molecules was slow, it could be enhanced by an additional annealing process using an embedded Au microheater. The outstanding mechanical flexibility of the graphene film ensures the stable sensing response of the device under extreme bending stress. Our large-scale and easily reproducible MLG films can provide a proof-of-concept for future flexible NO2 gas sensor devices.