Fermented soybean food is one of most economical and health food due to its valuable nutritional and medicinal attributes and have been consumed for centuries as flavoring ingredient in Korea. On fermented soybean food such as doenjang, aroma compounds are important property because they determine taste and grade of fermentation. This study investigated variety of aroma compounds of doenjang made from different soybean genotypes. Aroma compounds in twelve different doenjang made from two cultivar (Daewon and Taekwang) and ten elite lines were extracted by steam distillation extraction (SDE) method and analyzed by gas chromatography/mass spectrometry (GC/MS). Aroma compound were detected over 80 kinds in eight samples (Daewon, Taekwang, MY177, MY187, MY189, MY192, MY204 and MY205) and under 70 kinds in four samples (yeonchun1, MY188, MY203 and MY206). Among the detected aroma components, 47 compounds were assigned as aromatic compounds (21), long chain fatty acid (13), short chain fatty acid (5) and others (8) by the computer library (Wiley 7n program). The major aroma compounds in twelve different doenjang were benzaldehyde, benzeneacetaldehyde, 2-methoxy-4-vinylphenol, pyrazine type compounds, cysteaminesulfonic acid, palmitic acid, oleic acid and linoleic acid. Each doenjang made by same condition (microbe, temperature, aging time and etc) had a difference in ratio and variety of aroma compounds due to different components having soybean genotype.

It has been reported that bacterial diseases of domestic soybean are bacterial pustule, wildfire, bacterial blight and bacterial brown spot. Among these bacterial diseases, bacterial blight infected by Gram-negative Xanthomonas campestris pv. glycines had been the most issue in soybean diseases. In recently, issue in soybean disease was shifted to wildfire by Gram-negative Pseudomonas syringae pv. tabaci. When infected in nature, symptom of wildfire generally surrounded infected-area of bacterial blight and formed pustule in infective center. Because optimum growth temperature of Wildfire is lower than these of bacterial pustule, Wildfire caused damage to crop yield in later growth stage. In this study, we checked symptoms of bacterial diseases with growth stages of soybean. Also, we surveyed disease severity of single infection, simultaneous infection and sequential infection of bacterial pustule and wildfire.

A total of 162 accessions of Perilla germplasm collected from diverse habitats in South Korea. The oil content of the seeds varied from 19.8 to 49.6%, the average being 33.3%. Oil content showed wide ranges of variation. The fatty acid composition of the seed oil showed the following overall ranges: palmitic 4.5 to 8.1%, stearic 1.6 to 7.4%, oleic 13.4 to 23.6%, linoleic 9.5 to 19.5%, and linolenic acid 48.6 to 66.4%. No correlation was found between oil content and fatty acid composition. The results obtained in this study provide useful background information for developing new cultivars with a high oil content and different fatty acid. Several accessions could be used as parental lines in breeding programs aiming to increase perilla oil quantity and quality.

For the development of SSR marker system in Vicia villosa Roth, an enriched library was constructed by using a modified biotin-streptavidin capture method and the selected clones were sequenced with GS-FLX(454). Of 37,794 sequenced reads, we found that 8,474 reads (22.4%) were redundant, leaving 29,320 unique ones (77.6%). Among the unique clones, 17,174 reads (58.6%) were having microsatellite repeating motifs. Sequence analysis of all SSR-containing reads revealed a predominance of the di-nucleotide SSRs (62.5%). The tri-nucleotide and the tetra-nucleotide SSRs were 5.7% and 22.5%, respectively. As the di-nucleotide type, the AG/GA class of repeat motif was most frequently identified (55.0% of the total di-nucleotide SSRs), followed by the CT/TC class (19.5%), and the TA/AT class (12.1%). Among the tri-nucleotide SSRs, the AGT/GTA/TAG class of repeat motifs was predominant (22.2%), followed by the ACT/CTA/TAC class (17.8%). Among the tetra-nucleotide SSRs, the CTTT/TTTC/TTCT/TCTT class of repeat motifs was predominant (31.2%), followed by the AAAG/AAGA/AGAA/ GAAA class (19.9%). Finally, we designed 779 primer pairs from the flanking sequences of SSR containing reads. We are undertaking the analysis of polymorphisms using the diverse collected accessions of Vicia villosa Roth now. This newly developed SSR marker set shall provide a very useful tool for implementing molecular diversity assessment and population structure studies of Vicia villosa Roth onward.

overgrowth of soybean plants and thereby brings about lodging, decrease in pod number, damage by disease and insect pest, etc. This experiment was carried out to investigate optimum decapitation method in vinyl mulching soybean cultivation. Sinbuseoktae, local soybean variety was sown on May 25 and June 15 in 2009. Decapitation treatments(Non-decapitation, V8 stage-stem, V8 stage-apex, Flowering initiation stage, V8 stage-Stem+Flowering initiation Stage) were practiced on major growth stage on May25 seeding. Lodging did not occur in apex decapitation at V8 stage while other treatments had severe lodging. NAR from R5 to R7 stage was also highest in apex decapitation at V8 stage, which meaned optimum plant organ structure in utilizing light. On the other hand, flowering date and maturity date among treatments were similar. Seed yield in apex decapitation at V8 stage was highest with 274kg/10a, followed by flowering initiation stage with 249kg/10a. The apex decapitation at V8 stage had 26% yield increase as compared with the control of 216kg/10a due to heavy 100-seed weight.

This experiment was carried out to examine ecological response and soybean quality as affected by environmental cultivation for producing high seed quality in domestic soybean variety. The results are as follows: Under equal cumulative temperature condition, soybean plants grown in Muju showed longer days to flowering, which was an effect of the long day-length on high latitudes, and longer duration of reproductive stage as a result of low temperature within that period. Considering apparent seed quality, 100 seed weight of soybeans grown in Muju was heavier than Miryang. Ratio of seed crack and disease-damaged seeds was lower in Muju, and these parameters decreases as planting was delayed. The protein contents did not show significant difference in terms of altitude and planting date, however, crude oil contents were higher in Miryang. An opposite trend was observed in C18:1 and C18:3. In the fatty acid composition, the proportion of C18:1 decreased as seeding date was delayed, and was higher in Miryang. Opposite observations were obtained from C18:3. The anthocyanin contents were highest on June 10 planting and higher in Muju than in Miryang. Isoflavone content was higher as seeding date was delayed and is similar accross seeding dates in Muju. As a summary, for high seed quality production the optimum planting date was June 10, and Muju was more suitable region than Miryang.

The cultivation of green manure crop is considered as a good management practice by increasing soil organic matter and fertility levels. This experiment was conducted to improve the soil environment under rice-based cropping system at paddy soil (fine loamy, mixed, nonacid, mesic, family of Aeric Fluventic Haplaquepts) in National Institute of Crop Science (NICS), Korea in 2006 to 2007. The variation of soil temperature in green manure plots was lower than without green manure (control) during spring season (April to May). The temperature variation of no tillage plot (broadcast before rice harvest) was the lowest among treatments. After green manure cropping, the soil bulk density and porosity ratio were improved at the top soil. The production of green manure was the highest athairy vetch and barley mixture plot by partial tillage. However, mixture treatment had no improvement on soil organic matter. After rice cropping with green manure application, soil quality was improved such as soil physical properties except mixture treatment. Therefore, we suggest that soil quality should be improved by green manure cultivation under rice-based cropping system.

NIR spectroscopy combined with multivariate analysis after the appropriate spectral data pre-treatment has been proved to be a very powerful tool for judgment of the relative pattern of the objects that have very similar properties. In this study, 500 GMO soybean seeds and, 500 non-GMO ones were measured in NIR reflectance mode. Principal component analysis (PCA), and discriminant analysis (DA) were applied to classify soybean with different genes into two groups (GMO and non-GMO). Calibrations were developed using DA regression with the cross-validation technique. The results show that differences between GMO and non-GMO soybeans do exist and excellent classification can be obtained after optimizing spectral pre-treatment. The raw spectra with DA model after the second derivative pre-treatment had the best satisfactory calibration and prediction abilities, with 97% accuracy. The results in the present study show NIR spectroscopy together with chemometrics techniques could be used to differentiate GMO soybean, which offers the benefit of avoiding time-consuming, costly and laborious chemical and sensory analysis.

"Yeu", an Asiatic lily variety was released by the National Horticultural Research Institute (NHRI), in 2006. A crossing was made between Asiatic lily "Foxtrot", a orange colored cultivar, and Asiatic lily "A94-l" (White bird x Foxtrot), a orange colored cultivar in 1996. A promising line No. 9857 was selected in natural cultivation condition from the progenies of a cross combination between "Foxtrot" and "A94-1" in 1998. The flower characteristics and cultivation performance of "Yeu" were investigated from 2000 to 2005 named as Wongyo C1-85. The natural flowering time of "Yeu" was the middle of June, but year-round cut flower production was possible by storing the bulbs under -1 to -2℃ conditions. The flower has unspotted, upward facing, and orange petals without fragrance. The plant height at the natural flowering time is 50±5.7 cm. The width of flower is 12.6±0.5 cm. The weight and circumference of bulb are 18.4±6.8 g and 12.4±2.2 cm, respectively. For forcing culture, it is necessary to add calcium to the fertilizer or remove side scales to prevent leaf scorch. Botrytis disease should be properly controlled especially in wet season.

"Black Eye", an Asiatic lily cultivar was released by the National Horticultural Research Institute (NHRI) in 2006. A crossing was made between an Asiatic lily "La Toya", a purple colored variety, and an Asiatic lily "Nerone", a dark red colored variety in 1997. Selection was made in natural cultivation condition from the progenies of a cross combination between "La Toya" and "Nerone" in 2000. The flower characteristics and performance of "Black Eye" were investigated from 2001 to 2005 as Wongyo C1-89. The natural flowering time of "Black Eye" at rain shelter condition was the middle of June, but year-round cut flower production was possible by storing the bulbs at -1 to -2℃ condition. It has black spotted and upward facing dark red petals without fragrance. The plant height is 118 cm. The width of flower is 16.8 cm. The weight and circumference of bulb are 25.9 g and 13.7 cm, respectively. For forcing culture at winter season, it is necessary to add Ca++ to the fertilizer or remove side scales to prevent leaf scorch. Botrytis disease should be properly controlled especially during wet season.