This study examines the effects of restaurant attributes on customers' perceptions of price and brand reputation. Four restaurant attributes were used to represent restaurant quality. Multiple regression was conducted to measure the relationships between the four restaurant attributes and price and brand reputation. Further, an independent T-test was used to compare differences between perceptions of locals and tourists with regard to restaurant quality under two categories (price and brand reputation) after they selected a restaurant. Results of this study revealed that residency did not have a significant impact on customers' perceptions of price or brand reputation, whereas restaurant attributes did have an impact. Taste was a predictor of perceived price, whereas service and atmosphere were predictors of brand reputation. In addition, comparison of restaurant attributes between locals and tourists showed that sanitation and service were significantly different in accordance with price and atmosphere, whereas taste and service were significantly different in accordance with brand reputation. Locals showed higher means for each restaurant attribute compared to tourists.

Among hemipteran insects which is the most important insect vector of plant viruses, small brown planthopper, Laodelphax striatellus, transmits the rice stripe virus (RSV) causing rice stripe disease. For effective control of RSV, it is important to understand interaction between RSV and L. striatellus. Therefore, in this study, expressed sequence tag (EST) databases were generated based on 454 GS-FLX pyrosequencing for comparative transcriptome analysis between nonviruliferous and RSV-viruliferous L. striatellus. By comparing the two EST libraries, we showed that 108 host genes were significantly up-regulated and 28 host genes were significantly down-regulated in viruliferous insects. Interestingly, genes encoding ribosomal proteins were mainly up-regulated in viruliferous L. striatellus, whereas genes related to translation were concentrated in the downregulated cohort. These RSV-dependently regulated genes may have important function in the behavior of planthopper or the transmission of RSV.

Bacillus thuringiensis serovar mogi of a novel serogroup (H3a3b3d), which showed mosquitocidal activity against Anopheles sinensis and Culex pipiens pallens, was isolated from fallen leaves in Mungyeong city, Republic of Korea. In contrast to the complicated plasmid profiles of B. thuringiensis H3 serotype strains, the B. thuringiensis serovar mogi contained only megaplasmid (> 30 MDa) on which the toxin genes were occasionally located. Sequence analysis using 454-pyrosequencing revealed that the megaplasmid harbored at least seven putative cry genes, showing about 84%, 75%, 73%, 58%, 84%, 39% and 75% similarities in amino acid sequences with Cry27Aa, Cry19Ba, Cry20-like, Cry56Aa, Cry39ORF2, Cry8Ba and Cry40ORF2, respectively. These cry genes were cloned to the Escherichia coli-B. thuringiensis shuttle vector, pHT1K, and then introduced into an acrystalliferous B. thuringiensis Cry-B strain for further molecular characterization. To investigate the role of these genes in crystal production, the expression profiles of these toxin genes were analyzed by quantitative real-time PCR (qrtPCR) from the wild type strain as well as transformant strains. The results clearly indicate that the cry39orf2 was the dominant ingredient in the crystal. This novel 3a3b3d type strain, B. thuringiensis serovar mogi, could be used as a good resource for studying unknown mosquitocidal cry genes.

The baculovirus expression system is one of the most popular methods used for the production of recombinant proteins but has several complex steps which have proved inherently difficult to meet a multi-parellel process. We have developed a novel recombinant bacmid, bEasyBm that enabling easy and fast generation of pure recombinant virus without any purification step. In the bEasyBm, attR recombination sites were introduced to facilitate the generation of recombinant viral genome by in vitro transposition. Moreover, extracellular RNase gene from bacillus amyloliquefaciens, barnase, was expressed under the control of Cotesia plutellae bracovirus early promoter. Therefore, only when the barnase gene was replaced to gene of interest, the bEasyBm could replicate in host insect cells. When the bEasyBm was transposed with pDualBac-EGFP and pDualBac-LUC respectively, there were no non-recombinant backgrounds were detected from unpurified BmEasy-EGFP or BmEasy-LUC stocks. In addition, the resulting recombinant virus, BmEasy-EGFP, showed comparable level of EGFP expression efficiency with the plaque-purified recombinant virus, BmEGFP, which was constructed using bBmGOZA system. Based on these results, high-throughput condition for generation of multiple recombinant viruses in a time was established.

Varieties of Bacillus thuringiensis (Bt) crystal proteins, Cry proteins, have so far been found as one of the most successful biological control agents which are safe to natural environments for a long time. Recently, cry genes encoding these Cry proteins have been widely applied for construction of transgenic crops resistant to pest insects. In this study, through the 3D structure prediction and accompanying mutagenesis study for the Mod-Cry1Ac, 7 and 16 amino acid residues from domain I and II, respectively, responsible for its insecticidal activity against larvae of Spodoptera exigua and Ostrinia furnacalis were identified. To construct novel cry genes with improved insecticidal activity, we randomly mutated these 23 amino acid sequences by in vitro muti site-directed mutagenesis, resulting in totally 24 mutant cry genes. For further characterization, these mutant cry genes were expressed as a fusion protein with polyhedrin using baculovirus expression system. SDS-PAGE analysis of the recombinant polyhedra revealed that expressed Cry proteins was occluded into polyhedra and activated stably to 65 kDa by trypsin. In the further study, we plan to investigate their insecticidal activity against Plutella xylostella, S. exigua and O. furnacalis larvae.

본 연구는 베트남 하노이 지역에서 8가지 한국 토마토 품종 (핑크탑, 큐피랑, 슈퍼도태랑, 선레드, 선글러브, TP-7 플러스, 러블리 250, 광복)과 1가지 베트남 품종(FM 120)을 대상으로 봄-여름 및 가을-겨울 작기에 따른 수확량 및 과실 특성을 비교 분석하였으며, 그 결과는 다음과 같다. 1. 작기에 따라 초장, 경장, 주당 과실수 및 수확량(kg)은 유의성이 있었으며 가을-겨울 작기에서 높게 나타났다. 전체적으로 한국품종이 베트남 품종에 비해 높은 생육특성을 보인 반면, 과실수와 수확량에서는 베트남 품종이 우수하였다. 2. 토마토 과실의 주요 특성인 과장, 과폭 및 과중은 작기에 따라 유의한 차이를 보였으며 가을-겨울 작기에서 높은 수치를 나타내었지만 TSS 함량은 봄-여름 작기에서 높았다. 건물중과 비타민C 함량은 작기에 따라 유의한 차이가 없었다. 품종별 과실 특성은 한국 품종이 우수하였지만 비타민C 함량은 베트남 품종이 높았다. 3. 봄-여름 작기에서 한국품종의 경우 수확량 및 과실특성에서 가을-겨울 작기 재배에 비해 큰 감소를 보인 반면 베트남 품종은 큰 차이를 보이지 않았다. 따라서 본 실험에 이용된 8가지 한국 품종의 경우 봄-여름 작기보다는 가을-겨울 작기 재배가 적합할 것으로 판단된다.

A new model and resultant equation for the coagulation of acrylonitrile monomers in precipitation polymerization are suggested in consideration of the surface tension (γ) and cohesive energy density (ECED). The equation was proven to be quite favorable by considering figure fittings from known surface tensions and cohesive energy densities of certain organic solvents. The relationship between scale value of surface tension (γ/M) and cohesive energy density of monomers can be obtained by changing the coagulation bath component for effective precipitation polymerization of acrylonitrile in wet spinning.

We present the results from B-, R-, I-, J- and H-band observations of the NEP-Wide survey field. The NEP-Wide survey is an AKARI survey of the North Ecliptic Pole covering ~ 5 square degrees area. Our optical/NIR imaging supports the AKARI IR imaging data by providing a crucial coverage in the optical/NIR. The optical data were obtained in 2007 using the 1.5 m telescope and SNUCAM at Maidanak Observatory, Uzbekistan. The NIR data were obtained in 2008 with FLAMINGOS on the KPNO 2.1 m telescope. We used IRAF, SExtractor, SCAMP, and SWarp for reducing the raw data, I-band fringe pattern removal, astrometry, standard photometry calibration, and source detection. Our optical-NIR data reach the depths of B ~ 23.4, R ~ 23.1, I ~ 22.3, J ~ 21.05, and H ~ 20.64 AB mag at 5-sigma. Here, we present the astrometric accuracy, galaxy number counts, completeness, and reliability, as well as redshift tracks of some normal galaxies and quasars on the B - R vs. R - I color-color diagram. The photometric data are being used for identifying optical counterparts of the IR data provided by AKARI, studying their SEDs, and selecting interesting objects for spectroscopic follow-up studies.

Bovine brucellosis causes abortion and infertility. The authors conducted this study in order to determine pathological lesions of Korean native cows and fetuses who received experimental vaccination with Brucella abortus RB51 and were challenged with Brucella abortus 2308. Gross and histopathological lesions in endometrium and placenta were observed in cows of the vaccinated group. Twenty-five percent of pregnant cattle in the vaccinated group showed endometritis and placentitis, which was three times lower, compared with the non-vaccinated group. The pathological lesions in the uterus and placenta in both groups were consistent with previous reports. Therefore, vaccination in heifers using Brucella abortus RB51 may not provide adequate protection against infection with Brucella abortus virulent strain.

황해 경기만은 제4기에 반복된 해침과 해퇴로 4개의 해침-해퇴 퇴적체(DS-1, DS-2, DS-3, DS-4)를 형성하였다. 본 연구는 황해 경기만 퇴적체의 형성을 6개 퇴적단계로 나누어 설명한다. A 단계는 MIS-6 저해수면 시기로서 큰 규모의 해수면 하강으로 인해 대부분의 지역이 대기에 노출되고 광범위한 하도 침식 및 풍화작용의 영향을 받았다. 이어지는 B 단계는 MIS-5e 까지 빠른 해수면 상승과정에서 MIS-5 시퀀스의 하부 해침퇴적체가 형성되었고, 다음에 이어지는 MIS-5d부터 MIS-4 저해수면 시기까지의 C 단계에서는 MIS-5 시퀀스의 상부인 해퇴퇴적체가 만들어졌다. 다음의 D 단계는 MIS-4 저해수면 시기부터 MIS-3c 고해수면 시기까지로 하도 침식 및 하도 충전 구조로 이루어진 MIS-3 해침 퇴적체가 형성되었다. 다음의 E 단계에서는 LGM 시기까지 계속적인 해수면 하강이 있었고 외해쪽에서는 천해 기원의 MIS-3 해퇴퇴적체가 만들어진 반면에, 내륙쪽에서는 노출된 환경에서 하도 충전 퇴적체나 범람원 퇴적체가 형성되었다. 마지막 단계인 F 단계는 황해 전체적으로 홀로세 해침이 발생하였고, 이 시기에 외해쪽에서는 대륙붕 사질 퇴적체와 조석사주 퇴적체가 형성되어 고해수면 시기인 현재까지 퇴적이 일어나고 있다.

The sensitivities of PrP Sc detection methods, western blotting (WB), immunohistochemistry (IHC) and protein mis-folding cyclic amplification (PMCA) techniques were compared from brains, spleens and blood of mice challenged with PrP Sc of murine-adapted BSE strain 301C. PrP Sc was detected in the spleen from 30 dpi by IHC and at 60 dpi by WB. At 30 dpi, disease-specific signals of PrP Sc was observed in only two follicles of a single spleen. PrP Sc was detected in spleen at 10 dpi with PMCA after 5 rounds of amplification. Clinical signs were obviously shown from 240 dpi, and coincided with first detection of PrP Sc in brains by WB, IHC and PMCA after one round amplification. In addition, PrP Sc was also detected in blood at 60, 180 and 240 dpi with PMCA after 5 rounds of amplification. The FDC-M1 epitope, which appears in immature FDCs, and PrP Sc were detected in follicles first at 30 dpi, whilst the FDC-M2 epitope of mature FDCs was detected at 60 dpi. More FDC-M2 epitope and PrP Sc were detected in follicles as disease progressed. The CD21/35 epitope is expressed on both FDCs and germinal center B cells. The pattern of CD21/35 expressing cells was similar to but less dominant than that of FDCs.

In this study, we examined whether Hanganutziu‐Deicher (H‐D) antigens are important as an immunogenic non‐a1,3‐galactose (Gal) epitope in pigs with a disrupted a1,3‐ galactosyltransferase gene. The targeting efficiency of the AO blood genotype was achieved (2.2%) in pig fibroblast cells. A total of 1800 somatic cell nuclear transfer (SCNT) embryos were transferred to 10 recipients. One recipient developed to term and naturally delivered two piglets. The a1,3‐galactosyltransferase activity in lung, liver, spleen, and testis of heterozygote a1,3‐galactosyltransferase gene knockout (GalT‐KO) pigs was significantly decreased, whereas brain and heart showed very low decreasing levels of a1,3‐ galactosyltransferase activity when compared to those of control. Enzyme‐linked lectinosorbent assay showed that the heterozygote GalT‐KO pig had more sialyla2,6‐ and sialyla2,3‐ linked glycan than the control. Furthermore, the heart, liver, and kidney of the heterozygote GalT‐KO pig had a higher N‐glycolylneuraminic acid (Neu5Gc) content than the control, whereas the lung of the heterozygote GalT‐KO pig had Neu5Gc content similar to the control. Collectively, the data strongly indicated that Neu5Gc is a more critical xenoantigen to overcoming the next acute immune rejection in pig to human xenotransplantation.

In the transcriptome surveys of Laodelphax striatellus, several cDNA sequences showed a high level of similarities to the insect picorna-like virus genomes. Interestingly, there was no sequence similarity between picorna-like virus sequences from the RSV-viruliferous and those from the non-viruliferous L. striatellus. Picorna-like virus from the non-viruliferous L. striatellus was a geographical isolate of Himetobi P virus (HiPV). The genome of the HiPV was 9,272 nt in length excluding the poly(A) tail and contained two open reading frames (ORFs), which were separated by a 176 nt intergenic region that functions as an internal ribosome entry site (IRES). The 5' ORF encodes the non-structural proteins and the 3' ORF encodes the capsid proteins. The partial genomic RNA of the picorna-like virus from the RSV-viruliferous L. striatellus, LsPV-2, was 8,769 nt in length excluding the poly(A) tail and contained a single, large open reading frame (nt 1–8,535) encoding a 2,845 aa polyprotein. In terms of sequence similarity, identity, and genome organization, LsPV-2 resembled insect picornalike viruses belonging to the family Iflaviridae. A phylogenetic analysis based on RNA-dependent RNA polymerase (RdRp) sequence showed that LsPV-2 was most closely related to the deformed wing virus (DWV). The HiPV and LsPV-2 were incompatible each other in L. striatellus, suggesting that these two picorna-like viruses may have important functions in transmission of the RSV.

Plasmids from Bacillus thuringiensis have been implicated in pathogenicity as they carry the genes responsible for different types of diseases in mammals and insects. B. thuringiensis serovar mogi of a novel serogroup (H3a3b3d), which showed mosquitocidal activity against Anopheles sinensis and Culex pipiens pallens, was isolated from fallen leaves in Mungyeong city, Republic of Korea. In contrast to the complicated plasmid profiles of B. thuringiensis H3 serotype strains, the B. thuringiensis serovar mogi contained only megaplasmid (> 30 MDa) on which the toxin genes were occasionally located. Sequence analysis using 454-pyrosequencing revealed that the megaplasmid harbored at least seven putative cry genes, showing about 84%, 75%, 73%, 58%, 84%, 39% and 75% similarities in amino acid sequences with Cry27Aa, Cry19Ba, Cry20-like, Cry56Aa, Cry39ORF2, Cry8Ba and Cry40ORF2, respectively. These cry genes were cloned to the Escherichia coli-B. thuringiensis shuttle vector, pHT1K, and then introduced into an acrystalliferous B. thuringiensis Cry-B strain for further molecular characterization. This novel 3a3b3d type strain, B. thuringiensis serovar mogi, could be used as a good resource for studying unknown mosquitocidal cry genes.

ORF11 (ac11) of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a highly conserved baculovirus gene whose homologs are found in all lepidoteran Group I NPV, but its function is unknown so far. To determine the role of ac11 in baculovirus life cycle, ac11 knock-out mutant, Ac11KO, was constructed using the plasmid capture system (PCS). Real-Time PCR analysis showed that ac11 transcript was first detected at 6 h post-infection (p.i.) and accumulated to maximum at 48 h p.i., indicating that ac11 is belong to late gene. When the genomic DNA of Ac11KO was transfected into Sf9 cells, viral replication was restricted to a cell transfected originally. While viral transmission of the Ac11KO was not observed in Sf9 cells, production of budded virus (BV) in Sf9 cells transfected with Ac11KO was observed by transmission electron microscopy (TEM). These results suggest that the ac11 is essential for AcMNPV to produce infective BV.

Cicadas that produce loud calling songs for mate attraction can be a nuisance to city dwellers in Korea. The exuviae of final instars can be used for estimation of population density. To provide a key for species identification, we investigated morphological characteristics of final instar exuviae for four cicada species that are abundant in urban areas of central Korea: Cryptotympana atrata, Meimuna opalifera, Oncotympana fuscata, and Graptopsaltria nigrofuscata. Eight morphological characters were used for statistical analyses. The results of a general linear model showed that species and sex were significant for morphological characters and that mean values of most of the morphological characters measured were significantly different among four cicada species. C. atrata and M. opalifera were separated from the other species in most characters measured. Although O. fuscata and G. nigrofuscata were not distinguishable based on size-related characters, these two species differed in femoral tooth angle. Therefore, the exuviae of all four cicada species can be easily distinguished based on the morphological characters used in this study.

We developed two kinds of selective mosquito traps. The first selective mosquito trap called Mos-hole was developed with emitting carbon dioxide. The principle was that CO2 gas was obtained from burning liquid naphtha. The process principle was very unique that some heat and moisture which cannot be obtained by using dry ice could be generated additionally. It is the main cause to bring more mosquitoes. The trap is consisted of the acidic and CO2-baited suction system. CO2 was so powerful mosquito attractant gas, and the color and shape of a suction trap was also very important factors for improving the mosquito capturing efficiency. The trap burned naphtha in a rate of 1.3g/hr~3.0g/hr (CO2:50ml/min-110ml/min) and the efficiency of the trap was higher at the burning rate with the high number of female mosquitoes. The second mosquito trap called DMS (Digital Mosquito Monitoring System) is developed for reporting the number of captured mosquito automatically every day. This automatic reporting device called DMS uses CO2 gas as a mosquito attractant and it has a IR array sensor for counting mosquito. We established a sensor network with several DMSs and one server. The server collected the data of each DMS through Internet or CDMA RF communication system. This data were analyzed in the GIS pest prevention information system and were sometimes used as a reference for the next pest control activities. The DMS systems emit CO2 about 300cc/min. CO2 was very effective for attracting mosquito. DMS systems were very effective to count the number of mosquitoes at the certain areas. By using two kinds of mosquito traps together, we could determine the mosquito population size increase and decrease at certain areas