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        검색결과 621

        61.
        2022.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        고객의 ‘갑질’로부터 서비스 조직 구성원을 보호해야 한다는 논의가 활발하게 이루어지면서 고객의 비인 격적인 행동에 대한 관심이 높아지고 있다. 고객의 무례한 행동(customer incivility)로 인하여 서비스 조직 의 구성원들은 심리적으로 큰 어려움을 겪고 있으며, 우울증과 극단적 선택까지 고려하는 상황이 되었다. 이러한 점에서 조직은 고객의 무례한 행동의 부정적 결과를 인식하고 이를 개선하기 위한 노력이 필요하다. 본 연구에서는 고객의 무례한 행동이 스트레스를 통해 서비스 몰입과 이직의도에 미치는 과정에서 작용하 는 메커니즘을 확인하는 목적으로 수행되었다. 특히, 고객의 무례한 행동이 구성원들에게 미치는 부정적 효과를 보다 엄격하게 확인하기 위하여, 조직 내부에서 발생할 수 있는 직속상사의 비인격적 감독(abusive supervision)을 통제하였다. 가설검증을 위하여 2019년 5월에서 8월 사이 수도권 서비스직 종사자를 대상 으로 설문조사를 실시하였으며, 최종 205개의 설문을 기반으로 실증분석을 실시하였다. 본 연구의 분석 결과 고객의 무례한 행동은 구성원들의 서비스 몰입을 낮추고, 이직의도를 높이는 것으로 조사되었다. 또한 이 과정에서 스트레스가 매개 효과를 가짐을 확인하였다. 본 연구는 실증분석 결과를 바탕으로 이론적·실무 적 시사점을 논의하였으며, 향후의 연구 과제를 제시하였다.
        5,700원
        62.
        2022.05 구독 인증기관·개인회원 무료
        To obtain confidence in the safety of disposal facilities for radioactive waste, it is essential to quantitatively evaluate the performance of the waste disposal facilities by using safety assessment models. Thus, safety assessment models require uncertainty management as a key part of the confidencebuilding process. In application to the numerical modelling, the global sensitivity analysis is widely employed for dealing with parametric and conceptual uncertainties. In particular, the parametric uncertainty can be effectively reduced by minimizing the uncertainty of critical parameters in the safety assessment model. In this paper, the numerical model of each step disposal facility (Silo, Near surface, and Trench type) at Wolsong Low and Immediate Level Waste (LILW) Disposal Center is designed by using a two-dimensional finite element code (COMSOL Multiphysics). In order to determine the critical parameters for non-adsorbed nuclides such as H-3, C-14, Tc-99, we introduced the variance-based sensitivity analysis methodology of the global sensitivity analysis. In the case of Silo type, the density of waste is highly sensitive to the total leakage quantity of all nuclides. Additionally, the initial nuclide concentration of H-3 was identified as another important parameter of H-3. On the other hands, the mass transport coefficient showed a high contribution in C-14 and Tc-99. In other types of disposal facilities, the leaking properties of H-3 are significantly affected by the amount of infiltration water. However, C-14 and Tc-99 were found to be more sensitive to the density of waste.
        75.
        2022.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The mechanosynthesis route is a physical top–down strategy to produce different nanomaterials. Here, we report the formation of graphene nanoribbons (GNRs) through this route using carbon bars recovered from discarded alkaline batteries as raw material. The mechanosynthesis time (milling time) is shown to have an influence on different features of the GNRs such as their width and edges features. TEM revealed the presence of GNRs with widths of 15.26, 8.8, and 23.55 nm for the milling times of 6, 12, and 18 h, respectively. Additionally, the carbon bars evolved from poorly shaped GNRs for the shortest milling time (6 h) to well-shaped GNRs of oriented sheets forming for the longest milling time. Besides GNRs, graphene sheets (GNS) of different sizes were also observed. The Raman analysis of the 2D bands identified the GNS signal and confirmed the GNRs nature. ID/IG values of 0.21, 0.32, and 0.40 revealed the degree of disorder for each sample. The in-plane sp2 crystallite sizes ( La) of graphite decreased to 91, 60, and 48 nm with increasing peeling time. The RBLM band at 288 cm− 1 confirmed the formation of the GNRs. Mechanosynthesis is a complex process and the formation of the GNRs is discussed in terms of a mechanical exfoliation, formation of graphene sheets and its fragmentation to reach GNR-like shapes. It is shown that the synthesis of GNRs through the mechanosynthesis route, besides the use of recycled materials, is an alternative for obtaining self-sustaining materials.
        5,400원
        76.
        2022.02 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Fluorescent nanostructures based on carbon, or carbon dots, are attracting much attention and interest because of their diverse properties which can be applied in several fields of knowledge, such as optics, biomedicine, environmental research, among others. Such properties are in part, derived from its intrinsic luminescence from tunable functional groups. In this work, we produced carbon nanodots (CND) using agro-industrial residues, such as Lolium perenne and malt bagasse. The methods used were conventional hydrothermal syntheses and microwave-assisted hydrothermal synthesis. To the best of our knowledge, this is the first time that carbon dots synthesized from this ryegrass type are reported. The synthesis methods were one step (no catalyst, base, or acid were added for passivation), and the functional groups responsible for the luminescence and high solubility in water were identified by infrared spectroscopy, being mainly C=O, C–OH, C–N, and N–H. According to our theoretical studies, the C=O group introduced a new energy level for electronic transitions that can affect the emission properties. Fluorescence images of osteoblasts using CNDs were acquired and their chelating property towards Pb2+ and Cr6+ detection was tested.
        4,200원
        77.
        2021.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Anion assisted [4 + 2] cycloaddition reaction via indolo[2,3]-dienolate by reacting 1,2-dimethylindole-3-carboxaldehyde with aromatic nitriles using lithium diisopropylamide in terahydrofuran, a facile and convenient regiospecific route designed and developed for the synthesis of γ-carbolines.
        4,000원
        78.
        2021.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        We investigated the effect of a synthetic complement peptide C3a on the outcome of Brucella abortus 544 infection in a murine macrophage cell line RAW264.7 cell. First, we determined the highest non-cytotoxic concentration of the peptide in the cell line. We also found that the peptide significantly increased the growth of the bacteria at 8 and 24 h. Although the number of bacterial CFU was also elevated at 48 and 72 h, the increases were not significant as compared to controls. We further investigated the effect of C3a peptide on the growth of Brucella by pre-incubating the peptide at various temperatures and found that the effect was reversed at 24 h post-incubation suggesting that incubation of peptide at high temperatures including 65°C or 95°C could inactivate its action. This also could indicate the beneficial effect of high temperature during infection. Although several studies reported the inhibitory effect of different antimicrobial peptides including C3a, the present study preliminarily revealed that it had no positive contribution on the control of B. abortus 544 infection in vitro and indirectly to its receptor, CD88, which belongs to GPCR. Moreover, the encouraged further exploration of the effect of other similar peptides would be performed for the purpose of finding Brucella-host cell interaction for the control of disease progression.
        4,000원
        79.
        2021.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This study aims to investigate the effects of exogenous succinic acid (SCA) on Brucella (B.) abortus infection in macrophage RAW 264.7 cells and ICR mice. Firstly, the in vitro experiment was conducted by MTT cytotoxicity and bacterial internalization assay to evaluate the uptake of B. abortus into macrophage cells. Two non-cytotoxic concentrations of SCA demonstrated attenuated invasion of Brucella into macrophages at 30 and 45 min post- infection (pi). Secondly, ICR mice were treated with SCA and infected with B. abortus. On day-14 pi, spleen and blood serum were collected to evaluate the bacterial burden and total spleen weight as well as the production of cytokine/chemokine, respectively. The results showed that SCA treatment promoted bacterial growth and reduced the total spleen weight in mice. Furthermore, SCA treatment increased the level of IL-10 cytokine in the sera, while dampening the production of MCP-1 chemokine compared to the control. The results of bacterial load in spleen and spleen weight together with cytokine/chemokine production profile in the sera indicated that SCA induced the host anti-inflammatory response which is beneficial for the survival of Brucella. Therefore, these findings suggest that SCA contributed to host immunity against Brucella infection and the emerging potential topic-immunometabolism should be invested for further investigations.
        4,000원
        80.
        2021.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        In Nepal, about 73% of the vegetable production area is estimated to be covered by hybrid varieties. Most of the hybrid seeds in vegetable crops are imported from abroad. Considering the demand for hybrid vegetable varieties, the National Horticulture Research Centre (NHRC), Khumaltar, Lalitpur has started hybrid breeding of vegetables using a conventional approach since the fiscal year 2003/04. With this effort, the tomato hybrid ‘Srijana’ was developed and registered in 2010. This variety was estimated to cover about 35% of the total tomato cultivated area. Two tomato hybrids, namely ‘Khumal Hybrid Tomato-2 (HRA14 × HRD7) and ‘Khumal Hybrid Tomato-3’ (HRA20 × HRD2), showed good performance and were released in the National Seed Board in 2021. It was found that the heterosis for the yield was 87% in ‘Khumal Hybrid Tomato-2’ and 95% in ‘Khumal Hybrid Tomato-3’ with a potential yield of 70 MT/ha and 60 MT/ha, respectively, under open field conditions. Similarly, a comparative study of 7 tomato hybrids at different research stations was carried out and it showed that F1 (HRA13 × HRD7) could be a potential hybrid for the western region with a potential productivity of 116.8 MT/ha under a protected cultivation system. Hybrid breeding of cucumber was also initiated in the fiscal year 2007/08. A total of 12 crosses in cucumber were performed and two hybrids F1 (HRDCUC004 x RDCUC001), 107 MT/ha and F1 (HRDCUC009 × HRDCUC003) 150 MT/ha were found promising. Hybrid breeding in brinjal, hot pepper and bitter gourd has also been started. Furthermore, key problems and way forward to strengthen hybrid vegetable breeding in Nepal were also discussed.
        4,000원
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