Japanese pine sawyer beetle, Monochamus alternatus Hope (Coleoptera: Cerambycidae) is considered as a serious pest in pine trees. To develop an eco-friendly strategy to manage this forest insect, we collected entomopathogenic fungi from Korean soil and assessed their virulence against the adults of the insect in laboratory conditions. As a result, two isolates with conidial suspension (1.0×107conidia/ml), showed 87% and 90% mortality 12 days after fungal treatment, respectively. We assessed the potential of the fungi-derived destruxin and protease as additives to the fungal isolates, and they showed insecticidal activity via feeding and spraying treatments. Finally, we produced fungal conidia in massive solid cultures and formulated wettable powders, and now studying optimal conditions of oil-based formulation with two isolates. Based on these results, we are evaluating the control efficacy of the fungal agents against M. alternatus in field conditions.

Humic substances that can be obtained from coal resources such as leonardite in a bulk scale have been employed as crop stimulators and soil conditioners. The polymeric organics containing a variety of aromatic and aliphatic structures are known to activate plants in a multifunctional way, thus resulting in enhanced germination rate and abiotic stress resistance concomitant with induction of numerous genes and proteins. Although detailed structural-functional relationship of humic substances for plant stimulations has not been deciphered yet, cutting-edge analytical tools have unraveled critical features of humic architectures that could be linked to the action mechanisms of their plant stimulations. In this review article, we introduce key findings of humic structures and related biological functions that boost plant growth and abiotic stress resistance. Oxygen-based functional groups and plant hormone-like structures combined with labile and recalcitrant carbon backbones are believed to be critical moieties to induce plant stimulations. Some proteins such as HIGH-AFFINITY K+ TRANSPORTER 1, phospholipase A2 and H+-ATPase have been also recognized as key players that could be critically involved in humic substance-driven changes in plant physiology.

Roller Compacted Concrete Pavement (RCCP) is placed by roller compaction of a mixture of less cement and unit water content and more aggregates and provides excellent early strength development with the help of interlocking of aggregates and hydration. The unit cement content of RCC pavements accounts for 85% of conventional pavements, with low drying shrinkage. As low drying shrinkage leads to smaller crack widths than ordinary concrete, RCC pavements can help elevate reflecting crack resistance if applied to a base layer of a composite pavement system. In a composite pavement with an asphalt surface laid over a concrete base, pavement temperature change is important in predicting pavement performance. As movement of the lower concrete layer is determined by temperature depending on pavement depth, temperature data of the pavement structure serves as an important parameter to prevent and control reflecting crack. Among the causes of reflecting crack, horizontal behavior of the lower concrete layer and curling-caused vertical behavior of joints/cracks are considered closely related to temperature change characteristics of the lower concrete course (Baek, 2010). Previous studies at home and abroad about reflecting crack have focused on pavement behavior depending on daily and yearly in-service temperature changes of a composite pavement (Manuel, 2005). Until now, however, studies have not been conducted on initial temperature characteristics of concrete in composite pavements where asphalt surface is placed over an RCC base. Annual temperature changes of in-service concrete pavements go up to 60 ℃, and those of asphalt overlays become around the twice at 110 ℃. This study evaluated initial crack behavior of composite pavement by investigating pavement temperature by depth of an RCC base and analyzing joint movement depending on change to temperatures of continuously jointed pavements. Findings from the study suggest that in composite pavements and asphalt overlays, time of laying asphalt has an important impact on crack behavior and reflecting crack.

In recent years, pavement distresses have been caused by diverse factors such as spalling, deterioration of repaired sections, blow-up, and alkali aggregate reaction due to changing climate environment of a concrete pavement and its construction and maintenance conditions (supply of materials, increase in use of de-icers, etc,). As a leading repair method for deteriorated concrete pavements, partial-depth repair is implemented in accordance with guidelines of material properties for joints of a concrete pavement and field application evaluation systems, but still some of the repaired sections become deteriorated again at an early stage due to poor construction quality and failure of response to environmental impacts. Distresses that can be corrected with partial-depth repairs are largely divided into those of repair materials and of the existing pavement bonded to repair materials, and combined distress of repair materials and the existing pavement. Although re-repair methods should be different by distress type and scale than conventional pavement repair methods, appropriate repair methods and guidance for re-repairs have not been in place so far, and therefore currently, re-repair practices follow the existing manual of partial depth repairs. Therefore, this study evaluated concrete bond characteristics by removing method and repair scope for an experimental section of frequently distressed pavements to determine a re-repair scope and method for deteriorated partial depth repair sections of concrete pavement, the number of which has increased over time.

최근 봄철 기온이 높아짐에 따라 마늘 뿌리응애 피해가 늘어나고 있는 실정이다. 이러한 뿌리응애 피해를 최소하 하고자 발생양상과 22종의 약제에 대하여 살비효과를 조사하였다. 마늘 재배시기 중 뿌리응애의 발생은 충북 보은 등 5지역에서 2015년부터 2017년까지 3년간 3월부터 6월까지 뿌리응애 발생밀도를 조사하여 발생양상을 확인하였다. 뿌리응애는 2016년에 가장 많이 발생하였으며, 발생시기는 월동 후 3월 상순부터 발생하여 6월 중순경까지 지속적으로 밀도가 높아지는 양상을 나타내었다. 조사된 5지역 중 가장 높은 밀도로 조사된 보은지역은 6월 상순경 주당 13.3마리가 발생하였다. 뿌리응애에 대하여 우수한 살비효과를 나타낸 약제는 분무처리시 약제처리 10일 후에서 dimethoate, azocyclotin, abamectin, hexythiazox, cabosulfan, spirotetramat, benfuracarb가 90% 이상의 살비효과를 나타내었다

We have analyzed the transcriptome of Scolopendra subspinipes mutilans using RNA sequencing and identified severalantimicrobial peptide candidates. Among the peptides, named scolopendrasins, were selected based on the physicochemicalproperties of antimicrobial peptides via an in silico analysis. As a result, we evaluated the antimicrobial activities ofscolopendrasins against Gram positive and negative bacteria including Candida albicans by radial diffusion assay and colonycount assay. We also investigated the cytotoxicity of scolopendrasins through hemolysis assay. We found that the actionof scolopendrasins involved binding to the surface of microorganisms via a specific interaction with lipopolysaccharides,lipoteichoic acid, and peptidoglycans, which are components of the bacterial membrane. These results will provide a basisfor developing therapeutic agents such as peptide antibiotics.

This study was aimed to evaluate the dose-response the effects of nano-encapsulated conjugated linoleic acids(CLAs) on in vitro ruminal fermentation profiles. A fistulated Holstein cow was used as a donor of rumen fluid. Nano-encapsulated CLAs(LF, 5% of nano-encapsulated CLA-FFA; HF, 10% of nano-encapsulated CLA-FFA; LT, 5% of nano-encapsulated CLA-TG; HT, 10% of nano-encapsulated CLA-TG) were added to the in vitro ruminal fermentation experiment. In the in vitro ruminal incubation test, the total gas production on incubation with nano-encapsulated CLAs was increased significantly according to the incubation time, compared with the control(p<0.05). The tVFA concentrations on addition of LF and HT were significantly higher than that of the control(p<0.05). Thus, nano-encapsulated CLAs might improve the ruminal fermentation characteristics without adverse effects on the incubation process. In addition, the population of Butyrivibrio fibrisolvens which is closely related to ruminal biohydrogenation was increased by adding HT, while decreased by adding LF at 12 h incubation. These results showed that nano-encapsulated CLA-FFA could be applied to enhance CLA levels in ruminants by maintaining the stability of CLA without causing adverse effects on ruminal fermentation profiles considering the optimal dosage.

The purpose of the study was to investigate the strength of sokinetic muscle by the position of a volleyball players. Analysis and comparison of shoulder dynamic stability will be conducted according to rotational movement of the shoulder during spiking and serving amongst the various positions. Fifty professional Korean female volleyball players (age: 20~30), all different positions – attacker (left and right), center, setter, and libero were The concentric peak torque, strength ration of the internal and external rotation of the shoulder girdle for both dominant and non-dominant arms. Firstly, there were significant differences found for the strength ratio between the setter and the other positions in the dominant arm. On a second note, there was a significant decrease in shoulder dynamic stabilization for both the attacker and center in the dominant arm. However, there were no significant differences for the setter or the libero. This study suggests that the isokinetic muscle strengths of the volleyball players are different from each other.

Metformin (1,1-dimethylbiguanide hydrochloride), derived from French lilac (Galega officinalis), is a first-line anti-diabetic drug prescribed for patients with type 2 diabetes. However, the role of metformin in odontoblastic cell differentiation is still unclear. This study therefore undertook to examine the effect of metformin on regulating odontoblast differentiation in MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells. As compared to controls, metformin significantly accelerated the mineralization, significantly increased and accelerated the expressions of ALP and Col I mRNAs, and significantly increased the accelerated expressions of DSPP and DMP-1 mRNAs, during differentiation of MDPC-23 cells. There was no alteration in cell proliferation of MDPC-23 cells, on exposure to metformin. These results suggest that the effect of metformin on MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells, facilitates the odontoblast differentiation and mineralization, without altering the cell proliferation.

The purpose of this study was to compare and analyze the difference of the ankle joint movements during landing. Seven adult males voluntarily participated in the study and the average foot size of the subjects was 269.8 mm. Image analysis equipment and the ground reaction force plate (landing type) was used to measure th kinetic variables. As a result of this study, it was confirmed that the vertical ground reaction force peak point appeared once in the barefoot with forefoot, while two peak points appeared in the barefoot and functional shoe foot with rear foot landing. About ankle angle, fore foot landing ankle angle, the average with bare foot landing was -10.302° and the average with functional shoe foot landing was -2.919°. Also about rear foot landing, ankle angle was 11.648° with bare foot landing and 15.994° with functional shoe landing. The fore foot landing, ankle joint force analysis produced 1423.966N with barefoot and 1493.264N with functional shoes. But, the rear foot landing, ankle joint force analysis produced 1680.154N with barefoot and 1657.286N with functional shoes. This study suggest that the angle of ankle depends on the landing type and bare foot running/functionalized shod running, and ankle joint forces also depends on landing type.

The purpose of this paper is to examine the effect that Chinese tourists’ perceptions of beauty products and their friendliness toward Korean culture have on beauty tourism, particularly on Chinese tourists’ intent to purchase, re-purchase, and recommend beauty products. Between the 10th and 21st of June, 2016, a total of 277 questionnaires were distributed in Seoul, Busan, and Daegu using SPSS 21.0. Cronbach’s α was undertaken to test the reliability of the questions and an analysis of the frequency, factors, t-test, and Sobel test used in the study. Korean beauty was derived from two factors: “product favorability” and “product excellence and credibility.” Product favorability had a significant effect on the intent to purchase, as did participants’ friendliness toward Korean culture. Re-purchases and the intent to recommend beauty products were also significantly affected. In the relationship between the perception of beauty products and the intent to purchase, the study revealed partial mediation effects of the participants’ friendliness toward Korean culture on product favorability and complete mediation effects on product excellence and credibility. Friendliness toward Korean culture had partially mediated the effect that product favorability had on the intent to re-purchase and recommend. Tourists’ friendliness toward Korean culture had complete mediation on the effect that product excellence and credibility had on the intent to re-purchase and recommend. According to the Gender Equality and Family Act, the difference between buying and selling beauty depends on the difference between purchase and intentions. Friendliness toward Korean culture has become an important variable thanks to product superiority and reliability.

To obtain information on the sanitary of indoor environment in greenhouses used for shiitake cultivation, bacteria associated with larvae and imagoes of Sciaridae flies, pest to shiitake mushroom, were isolated and identified. A total of 1,048 bacterial colonies were isolated from the flies’ larvae and 984 bacterial colonies were isolated from the flies’ imagoes. Based on molecular analysis of the 16S rDNA sequence, Achromobacter xylosoxidans and Tetrathiobacter kashmirensis of β-proteobacteria, Enterobacter asburiae and Raoultella ornithinolytica of γ -proteobacteria, Curtobacterium sp. and Microbacterium thalassium of Actinobacteria, and Penibacillus taichungensis of Firmicutes were identified from the colonies of the flies’ larvae. While, Bacillus megaterium, B. thuringiensis, Lysinbacillus sphaericus and L. fusifomis of Firmicutes, Microbacterium thalassium and Citricoccus parietis of Actinobacteria, and Enterobacter asburiae of γ-proteobacteria were identified from the flies’ imagoes. Some of the isolated bacterial species were known be human pathogens. Overall, the results of this study suggested that mushroom fly carrying human pathogenic bacteria is one of sources impact on the sanitary of indoor environment of greenhouses used for shiitake cultivation.

본 실험은 토마토 플러그묘의 접목활착율과 묘소질 향상을 위한 적정 상토 내 수분함량을 구명하기 위하여 수행되었다. 시험구는 접수와 대목의 상토내 수분함량을 각각 3처리구(고, 중 저)씩, 총 9개의 조합을 설계하여 각 처리구별로 접목활착율과 묘의 생육을 조사하였다. 접수의 상토내 수분 함량의 차이는 접목 활착율에 통계적 유의차는 나타나지 않았으나, 대목의 수분함량은 접목활착율에 영향을 미쳐 수분함량이 낮아질수록 활착율도 저하하는 경향을 보였다. 수분 함량에 따른 묘소질도 접목활착율과 유사한 경향을 보여 접수의 수분함량차이는 생육지표에 큰 영향을 미치지 않았으나, 대목에서는 잎(엽수, 엽장, 엽폭)을 제외한 초장, SPAD함량, 경경은 수분함량의 차이에 따라 유의적 차이를 나타냈다. 묘의 충실도는 상토내 수분 함량이 접수는 중간, 대목은 높은 조합에서 가장 좋게 나타났다. 근권부의 생육에서도 대목의 수분함량이 주로 영향을 미쳐, 수분함량이 저하될수록 전 뿌리 표면적, 전 근장, root tip수 모두가 감소되는 것으로 나타났다.

The anti-proliferative efficacy of t,t-conjugated linoleic acid (t,t-CLA), c9,t11-CLA, and t10,c12-CLA was compared in several human cancer cell lines. Gastric NCI-N87, liver Hep3B, pancreas Capan-2, and lung NCI-H522 cancer cells were incubated with 50 μM CLA isomers over a period of 6 days. The t,t-CLA inhibited the growth of all cancer cell lines to different extents, but c9,t11-CLA and t10,c12-CLA inhibited or stimulated the growth of the cancer cell lines. NCI-N87 cells were the most sensitive to growth inhibition and apoptosis from all CLA isomers tested. In NCI-N87 cells, CLA isomers reduced the release of arachidonic acid (AA) via the inhibition of cytosolic phospholipase A2 (cPLA2 ) activity, consequently reducing the production of PGE2 through the inhibition of cyclooxygenase-2 (COX-2). The efficacies of CLA isomers were in the following order (from most to least effective): t,t-CLA, t10,c12-CLA and c9,t11-CLA. Overall, these results imply that the anti-proliferative efficacy of t,t-CLA on cancer cells, especially NCI-N87 cells, was greater than other CLA isomers due to its induction of apoptosis through the inhibition of cPLA2 and COX-2 activities.