This study was investigated to evaluate germination rate of Astragalus membranaceus B. in Korea as affectedby storage temperature, germination temperature and storage period of seed. The highest germination rate was obtainedfrom condition of 25℃ in germination temperature. Seeds were stored at −20℃ and 5℃ for 8 weeks has showed higher ger-mination rate than one at room temperatures. The germination rates showed significantly difference by harvested year of2010, 2011 and 2012. The seed of A. membranaceus in harvested year of 2011 and 2012 had germinated well. On the otherhand, seeds in harvested year of 2010 were not nearly germinated. Consequently, the longer storage period after seed har-vest lower germination rate and seed vigor as well.

억새 수집종의 초장 길이를 조사한 결과, 개화 전과 후 전체 생육기간 동안 200 cm 이상 우량한 생육을 나타낸 개체 자원은 India에서 수집한 억새 MS013이며, 개화 후에는 MS005, MS010, MS011, MS014 억새도 200 cm 이상의 생육을 보여주었다. 대부분의 억새 수집종의 경우 분얼수가 2-3개 정도로 나타났다. 엽장의 길이가 100 cm 이상인 수집종은 MS001, MS002, MS005, MS012, MS013, MS014로 6종이며, 엽폭은 1-2 cm 사이가 63%이상으로 대부분의 수집종들이 이에 속하였으며, MS002, MS003, MS005 억새의 엽폭은 2 cm 이상으로 잎이 넓은 것을 확인할 수 있었다. MS005 억새는 초장, 간경, 마디수, 분얼수 뿐만 아니라, 엽장, 엽폭에 있어서도 다른 지역에서 수집한 억새보다 수치가 높은 것을 확인하였으며 바이오에너지용 억새로서의 가치가 있다고 사료된다.

본 연구는 우리나라에서 자생 및 재배되고 있는 들깨와 차조기의 재배형 및 잡초형 계통들에 대하여 종자발아에 대한 변이 양상을 구명하기 위해 재배형 들깨 102계통, 잡초형 들깨 41계통 그리고 잡초형 차조기 19계통들에서 측정된 종자발아율 및 발아세는 다음과 같다. 1. 재배형 들깨는 저온처리 조건에서 1%에서 99%의 발아율을 나타내어 평균 73%±24를 나타내었으나, 비저온처리조건에서는 0%에서 98%의 발아율을 나타내어 평균 45%±26를 나타내었

본 연구는 우리나라와 중국 그리고 파키스탄에서 수집한 재래종 조 계통들에 대하여 형태적 특징에 의한 유전적 변이성 연구를 수행하였다. 그 결과 우리나라 및 중국, 파키스탄의 조 계통들은 다음과 같은 형태적 변이를 나타내었다. 1. 형태적 특성조사에 의하면, 우리나라에서 수집한 조 계통들은 중국과 파키스탄에서 수집한 계통들보다 출수기가 늦었고, 초장이 크고, 이삭이 긴 특성을 나타내었다. 반면에 파키스탄에서 수집한 계통들은 출수기가 빠르고, 초장이 작고,

The aim of this investigation was to examine the influence of extrusion on dietary fiber profile and the contentof bioactive compounds, rutin and quercetin in young sprout, whole seed, and matured stem of Tartary buckwheat.WSI(water soluble index) is increased by a function of both screw profile and process temperature, compared to control indifferent parts of Buckwheat. Also, WSI of ME is increased more than 5.2 times in grain, compared to that of control. Theeffect of precooking by extrusion on the dietary fiber profile of buckwheat flour was evaluated. Precooking by extrusion sig-nificantly increased SDF in flour, although in most cases extrusion decrease in TDF a little. The thermo-mechanical treat-ment undergone by the buckwheat flour during extrusion led to redistribute part IDF fraction to SDF, leading to an increasein the latter. The content of rutin was increased about two fold in extruded flour of sprout, compared to in control. Thisincrease maybe why these compounds are released from cell wall by high shear processing under high temperature.

Objectives The objective of our research was to establish the gene transformation, expression and characterization system in transgenic Codonopsis lanceolata. Materials and methods Agrobacterium tumefaciens strain LBA 4404/ binary vector pYBI121Regeneration of transgenic shoots: MS medium supplemented with 0.1 mg/ℓ NAA and 1 mg/ℓ BAP, 3% sucrose and 0.8% agar at pH 5.8. Agrobacterium cell density OD 600 between 0.8 and 1.0, Infection: 5 minutes DNA isolation and Polymerase chain reaction: DNA was extracted from young leafs excised from kanamycin resistant shoots. Two primers used for PCR amplification of the 700 bp of the npt II gene were N 1 (5′ GAA GCT ATT CGG CGG CTA TGA CTG 3′) as a sense primer and N 2 (5′ ATC GGG AGC GGC GGC GAT ACC CTA 3′) as a anti sense primer. Result and Discussion Adventitious shoots regenerated 3 weeks after Agrobacterium infection on regeneration medium containing 0.1 mg/ℓ NAA and 1 mg/ℓ BAP, 100 mg/ℓ kanamycin 250 mg/ℓ cefatoxime. Numerous adventitious shoot inductions of putative transformants were observed from the cut surface of explants which initially resembled knob like structure and later developed into new plant. PCR analysis of showed the expected bands of npt II gene. PCR analysis was carried out to confirm the insertion of the npt II gene in the genome of transformed plant. The expected amplified npt II fragments of size 700 bp was found in the T0 transformed plants, indicating the integration of npt II gene.