Successful cryopreservation of bovine oocytes is a very important technology for research and commercial applications. However, the survival and development rate of vitrified-thawed oocytes is lower than non-vitrified oocytes. Hydroxypropyl Cellulose supplementation (HPCs) has extremely high viscosity, which permits transitions to a glassy state at low temperatures. This characteristics of HPCs have been reported to help the survival of human oocytes. In this study, we investigated the survival rate, fertilization rate and ROS levels to confirm the effect of cryoprotectant solutions with HPC for oocyte vitrification in bovine. For vitrification, bovine MII oocytes were pretreated with EG10 (added 0, 10, 50 and 100 ㎍/㎖ HPC) for 5 min, exposed to EG30 (added 0, 10, 50 and 100 ㎍/㎖ HPC) for 30 sec, and then directly plunged into LN2. Thawing was taken by 4-step procedures [1 M sucrose and 10% FBS added D-PBS (SFD) -> 0.5 M SFD -> 0.25 M SFD -> 0.125 M SFD] for 1 min, respectively. After thawing, oocytes were washed with TL-HEPES, incubated in a droplet of previous cultured IVM medium for 1 h to recover. IVF drop (44 ㎕) contained 10 vitrified-thawed oocytes with sperm concentration of 1 × 106 cells ㎖, and then 2 ㎕ heparin and 2 ㎕ PHE were added. At 2 days after IVF, cleaved embryos were cultured in CR1aa + 3 mg/mL FAF-BSA for 48 h and cultured in CR1aa + 10% FBS for 4 days. In the results, in vitro survival rate of bovine vitrified-thawed MII oocyte was significantly higher in 50 (85.5%) and 100 ㎍/㎖ (80.2%) HPC groups than 0 (71.2%) and 10 ㎍/㎖ (71.3%) groups (p<0.05). The ROS level was lower in 50 ㎍/㎖ HPC group than in control group. After in vitro fertilization, cleavage rate and blastocyst development rate were not significantly different among treatment groups. Therefore, these results indicated that HPC treatment has a positive effect on the survival of vitrified-thawed bovine oocytes.

In the current study, a total of 102 common Todarodes pacificus squid caught in the East Sea were investigated for parasitological research. The results revealed that 33 (32.35%) out of 102 squid were infected by Nybelinia surmenicola, the mean intensity was 5.58 parasites per squid, and the maximum abundance was 11. Morphological analysis using a field emission scanning electron microscope showed the characteristic features of N. surmenicola. Molecular identification based on the 28S rRNA gene confirmed the isolated parasite as N. surmenicola, while phylogenetic analysis revealed that N. surmenicola isolated in this study was clustered with N. surmenicola isolated from Japan. This is the first report of phylogenetic characterization of N. surmenicola isolated from Korea.

본 연구는 지속가능한 유기농업의 실천을 위하여 국내 작부체계와 농업환경에 적합한 한국형 현장적용 기법을 구축하고자 수행하였다. 본 연구의 기본 개념은 ‘Natural Enemy in First (NE가 먼저)’로 해충발생시기의 예찰 없이 주 작물을 정식함과 동시에 천적과 보조식물을 혼합 적용해서 해충발생 이전에 천적을 포장에 먼저 정착시키는 생물적 방제기법이다. 시설재배 딸기에서 칠레이리응애, 사막이리응애, 콜레마니진디벌과 천적의 서식처 3종을 주 작물의 정식과 동시에 적용한 결과, 관행방제구에 비해 80%이상의 해충 밀도억제효과를, 천적 단독처리구에 비해 3배 이상의 높은 천적의 밀도를 확인할 수 있었다. 또한 포장 환경과 천적의 방사방법에 구애받지 않고 천적의 해충 방제효과를 극대화시킬 수 있는 라인을 적용한 결과, 약제와 천적 혼합처리구에서 라인을 적용하지 않은 처리구에 비해 해충의 밀도가 급격히 감소하고, 천적의 밀도는 2배 이상 높게 유지되는 것을 확인할 수 있었다.

애무늬고리장님노린재(Apolygus spinolae)는 포도원에서 생장 초기부터 신초를 흡즙 가해하여 잎의 기형화, 화진형상, 열매의 소립화, 기형, 열과 등의 피해를 일으킨다. 2017년과 2018년도에 경기도 화성 포도원의 캠벨얼리 품종을 대상으로 가온 하우스, 무가온 하우스, 비가림 재배, 노지 재배의 재배 양식과 생육단계별 애무늬고리장님 노린재의 발생 양상을 조사하였다. 재배양식별 피해는 1월부터 가온을 시작하여 7월 초순에 수확하는 조기가온 하우스와 3월부터 보온을 시작하여 8월 중순에 수확하는 무가온 하우스에서는 피해가 5% 미만으로 미미하였다. 그러나 비가림 및 노지재배에서는 잎과 열매의 피해가 관찰이 되었고, 특히 무농약 노지재배에서는 2017년도 27.6%, 2018년도 36%의 피해가지율을 나타내었다. 노지재배에서 피해 발생은 캠벨얼리 발아기 (BBCH(생물계절코드) 10)부터 이전 해에 포도나무 눈의 겉 인편 사이에 산란된 월동알이 부화하여 6 ~ 7엽(BBCH 16 ~ 17) 전개기까지 어린잎과 열매를 흡즙하여 피해를 주는 것으로 조사되었다. 애무늬고리장님노린재 온도 적산 모델에 따른 노지재배과원의 최적 방제시기는 월동알 부화 시기에는 2017년 4월 26일 (BBCH 11), 2018년 4월 30일(BBCH 11)이였고, 1세대 성충 출현시기에는 2017년 6월 5일 (BBCH 71), 2018년 6월 8일 (BBCH 74)로 예측되었다. 즉, 월동알 부화시기는 캠벨얼리 전엽 초기(BBCH 11), 1세대 성충 출현 시기는 과립 착과 초기(BBCH 71)로 예측되었다.

Gonggeom-ji is designated wetland protected area by the Ministry of Environment of Korea in 2011 because of it’s high biodiversity and historic value. It contains a reservoir, paddy and forest site which has diverse niches for insects. Three transect lines of 50m were designated. Data were collected in May, October, November in 2017 represented as seasons for spring, summer and autumn respectively. Quantitative methods were conducted along each transect line by sweeping and pitfall trap. Based on this study total of 1079 individuals of insect fauna were collected, representing 170 species in 60 families and 8 orders. The highest diversity, richness and evenness index were observed in the forest site in May(4.77, 8.6 and 0.91 respectively) and the highest dominance index was observed in the forest site in November(0.64). The highest similarity index was observed in the reservoir site in May and August(0.519).

This study aimed to identify the effects of kinesio taping (KT) applied in a proprioceptive neuromuscular facilitation (PNF) pattern on the pain, weight-bearing distribution (WBD), and walking ability of knee osteoarthritis (KOA) patients. Thirty women with KOA were randomly allocated to a control group (n=15) with KT at the quadriceps only, and a PNF pattern group (n=15) with KT at the quadriceps and gastrocnemius muscle. Pain intensity was measured using a visual analogue scale during walking. In addition, WBD, and walking ability were measured before and 30 minutes after KT application. The VAS significantly reduced in both groups after the intervention (p<.05). WBD (p<.05, ES=.32) and walking ability (p<.05, ES=.38) showed a significant change in the PNF pattern group, and in the inter-group comparison, the PNF pattern group showed a significant difference compared to the control groups. These results demonstrate that KT application with PNF pattern effectively attenuate the pain and improves WBD and walking ability in KOA patients.

국립원예특작과학원에서 2011년 거베라 신품종 ‘Pink Melody’를 육성하였다. ‘Pink Melody’는 2007년 10월부터 2007년 12월까지 분홍색 반겹꽃 ‘Eta’와 ‘Pascal’을 교배하여 획득한 97개체의 실생계통으로부터 2008년부터 2011년까지 4년 동안의 개화 및 생육특성검정을 거쳐 선발된 절화용 대륜 거베라 품종이다. ‘Pink Melody’는 초세강건한 녹색화심의 파스텔톤 분홍색(RHS R55D) 반겹꽃 대륜계 품종이다. 꽃은 평균 직경이 11.4±0.9cm이고, 내부설상화와 화반의 직경은 각각 7.3±0.8cm와 2.6±0.5cm, 외부 설상화의 길이와 폭은 각각 5.3±0.4cm와 1.0±0.1cm로 꽃잎이 비교적 좁고 긴 도란형이다. 꽃대는 꽃목직경이 상부 4.2±0.4mm, 중부 6.4±0.8mm 정도로 강건하며, 꽃대의 길이는 45.2±5.5cm이다. 절화수명은 10.8±1.6일로 대조품종 ‘Pascal’보다 약 1.5일이 길며, 연간 채화량은 52.9±4.3본/주로 대조품종보다 5.0본/주 많은 다수성 품종이다.

19세기말 Mycobacterium avium subsp. paratuberculosis(Map)이 요네병(Johne’s disease)의 원인균임이 밝혀진 이후, 불현성 감염된 동물들의 국제적 이동은 요네병을 전 세계로 퍼뜨리기 시작하였다. 이러한 요네병이 축산분야에 나타남과 동시에 새로운 형태의 대장염으로서 요네병과 같은 증상을 나타내는 질병(크론병)이 사람에게서도 나타나기 시작하였다. 그러나 Map이 이러한 새로운 대장염의 원인균이며 인수공통전염병의 원인체라는 인식은 20세기 후반 이러한 질병을 앓고 있는 사람의 조직으로부터 Map을 검출할 수 있게 되고서야 나타나기 시작했다. 본 총설은 어떻게 Map이 축산분야와 사람의 공중보건 측면 에서 심각한 문제를 야기시키게 되었고, Map 감염에 의한 대장염 환자의 치료가 어떻게 발전되어 왔는지 간단히 요약하고, 축산에서 Map의 통제를 위한 새로운 백신개발 전략에 대하여 소개한다.

The key objectives of the research is to examine the impacts of consumption community and underlying motivations for joining consumption communities in consuming masculine products among Korean female consumers. This study is the first to link a consumption community to social identity theory. To find an answer to our research questions, the study uses Korean Harley Davidson female riders who ride the American heavy motorcycle in a consumption community to answer the research questions. Harley Davidson is an icon of masculine products due to the size and the powerful engine of the motorcycle. A hermeneutic approach is adopted to understand Korean female consumers’ motivations in joining consumption communities and their perceptions about riding a Harley Davidson motorcycle. A hermeneutic method uses personal in-depth interviews to elicit the behaviors, values, and motivations that researchers are trying to understand (Kozinets, 2001; Thompson et al., 1994). The hermeneutic method is appropriate when researchers are attempting to understand motivations, values and beliefs deeply embedded in a person’s mind (Thompson et al., 1994).

아케이드 슈팅게임은 과거 1970년대 후반부터 1990년 후반까지 대중적으로 큰 인기를 끌며 게임시장을 주도했다. 하지만 2000년대 이후 많은 PC와 콘솔의 보급으로 아케이드 게임이 급속도로 쇠퇴하기 시작했다. 하지만 최근 국내에서 하락세를 보였던 아케이드 게임이 크게 상승세를 보였고 앞으로도 점점 상승할 가능성이 높아 보인다. 따라서 본 논문은 아케이드 게임의 특징인 빠른 손동작을 최신 기술과 접목시켜 새로운 입력 방식을 개발함으로 더 높은 효용성을 끌어내어 검증 한다. 기존에 슈팅게임에서의 제스처 인터페이스 사용은 이미 그 효용성이 검증되었다. 다만 좀 더 복잡한 완성형 게임에서의 적용하기엔 미흡한 부분들이 있다. 이에 슈팅게임들의 요소를 좀 더 세분화하여 분석 후 각 요소들을 제스처에 매핑하였다. 기존 게임에선 조이스틱을 손으로 흔들며 진행하는게 일반적인 방식으로 본 게임에선 사용자가 직접 손을 상하좌우로 움직여 조작한다. 또한 진행에 필요한 스킬과 이벤트들을 각각의 매핑 된 제스처 로 인터랙션 한다. 개발된 인터페이스는 슈팅게임 'Cure'에 적용하여 G-Start, HCI creative Award 등에서 전시되었다.

The current construction and maintenance guidelines applied to airport pavement in Korea are those of the International Civil Aviation Organization (ICAO), the International Air Transport Association (IATA), and the Federal Aviation Administration (FAA). In order to consider local conditions of airports in Korea, more specific details should be addressed in those guidelines. For example, the design and construction for pavements at airports in Korea follow the specifications of materials for general roads or foreign airport pavement guidelines, as there is no design manual or guideline for the granular base and subbase materials for airport pavement in Korea. In such circumstances, the likelihood of premature failure or accelerated damage increases, as the loading from airplanes is not fully taken into account or the local environmental characteristics are not considered. In addition, concerns in public facility drainage systems have been rising recently in line with the increase in the frequency and scale, caused by the global abnormal-temperature phenomenon, of localized torrential rain and snow. For airport runways, measures to maintain swift drainage systems are especially necessary to ensure safety and prevent flight delays. In this study, the appropriate moisture content and pavement method are analyzed by applying porous concrete developed for a cement-treated base course for securing permeability of airport pavement at an actual construction site. In addition, on-site construction testing was performed to determine the appropriate compaction method and the curing method to minimize cracking by using a compaction facility. To determine the optimal moisture content, a quality-control was performed by measuring the moisture content of porous concrete produced at a batch plant. For this purpose, a speed moisture test (ASTM D 4944) was performed on site because the unit-water content of the porous concrete affects its compaction and finishing. Before compaction, a grader was used to remove fragments on the subbase and then a tandem roller was used to level and compact. After compaction, the porous cement-treated base course, called porous concrete, was placed using an asphalt finisher. The mechanical properties and durability of the porous cement-treated base course with a variation of a degree of compaction: noncompaction, tandem roller moved back and forth once, three times, and five times. The pavement was covered with vinyl according to the curing guidelines suggested by the Korea Expressway Corporation’s highway construction specifications, to prevent evaporation from porous concrete that has relatively low moisture content. After curing, the core was collected to analyze the compressive strength, permeability coefficient, porosity, and freeze–thaw resistance characteristics.

As a surface treatment method of concrete pavement in Korea, transverse tinning has initially been applied to improve drainage and friction at first. Since 2003, in order to reduce the noise of concrete pavement, longitudinal tinning have been studied and proved its superiority. Therefore, Korea Expressway Corporation (KEC) has applied this as surface texturing method in concrete pavement from 2008 to the present. However, the lateral vibration during vehicle running has been continuously raised in the longitudinal tinning section, and lots of efforts have been made to solve this problem. Nonetheless, complaints still occur intermittently in some sections. In this research, three sections in which complaints occur and three test sections were chosen to evaluate characteristics of lateral vibration between tire and longitudinal texturing. Based on the literature survey on the correlation between longitudinal texturing and tire, evaluation of the site and FEM analysis of each road surface were carried out. As a result of the analysis, the lateral vibration occurred most frequently in the random tinning section compared to other sections, and it was relatively few at the other improved tinning of the test section. Further studies on various tire types and timing intervals in the future will provide a standard of longitudinal texturing, which will reduce lateral vibration.