The ladybird beetles have been used as biological control agents against several pest species. The aim of this study is to evaluate the compatibility between ladybird beetles and an entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin AAD16. The virulence of AAD16 strain was compared with that of commercial GHA strain on three developmental stages of two coccinellids; Harmonia axyridis Pallas and Chilocorus stigma Say. The topical application method was used for both adult and larval stage while dipping method was used for egg stage. The LT50 and mycosis rate for all life stages of two ladybird beetles tested were not significant between AAD16 and GHA strain. In larval stage, the mycosis rates of H. axyridis and C. stigma were 36 and 40% and 63 and 60% in AAD16 and GHA strain. In adult stage, the mycosis rates of H. axyridis (male and female) and C. stigma (unsexed) were 20, 23, and 23% and 26, 30, and 30% in AAD16 and GHA strain, respectively. Therefore, the two predatory coccinellids could be compatible with these two fungus strains due to their relatively lower mycosis rate.

Naturally occurring left ventricular hyperplasia is a rare but lethal disease. There are very few reports of this cardiac disease in captive nonhuman primates. In a colony of Macaca mulatta (Rhesus monkey) at California National Primate Research Center, a large number of rhesus macaques were diagnosed by autopsy with naturally occurring left ventricular hypertrophy without obvious underlying diseases over a 22-year period. The confirmatory diagnosis of ventricular hypertrophy was based on findings of notable left ventricular concentric hypertrophy with reduced left ventricular lumen, which is very similar to human ventricular hypertrophy cases. This report discusses an 11-year-old Macaca fascicularis monkey (Cynomolgus monkey, crab-eating macaque), weighing 2.95 kg, that was presented for enrollment in a pharmacokinetic (PK) study. During the PK experiment, the monkey died following a sudden decrease in percutaneous oxygen saturation and heart rate. Gross and histological examinations of the heart were performed. On gross pathology, the left ventricular wall was thickened, and the chamber lumen was reduced. In histopathological examination using hematoxylin- eosin and Masson-trichrome stains, fibrosis and myocyte disarray were not observed, but an increased cell density, compared to the normal heart, was confirmed. The autopsy results confirmed left ventricular hyperplasia as the major cause of death.

Currently, the cut flower market seems to be an export revenue in the world trade of the flower industry. The amount of exported cut flowers is impressive with increased production in various countries, especially in Korea. In the Fourth Industrial Revolution, automatic technologies are continuing to develop agricultural effective tools in the challenge of digital innovation. Thus, the low production costs set up in the field, greenhouse, or smart farm set up, and the short time of harvest must be considered within a few months. The postharvest quality of cut flowers presents freshness and long vase life, and the tools for postharvest handling are expected to optimize these. This review highlights the most important factors improving postharvest quality of cut flowers, the potential standard applicable techniques for commercial handling outlines of cut flower vase life, and recommendations for improving postharvest handling in the flower industry.

After harvest, Dolcetto cut roses were immediately exposed to air for 0 (E-0), 1 (E-1), 2 (E-2), and 3 h (E-3) and then placed under tap water for recovery. We determined the effect of air exposure time on vase life, water relations, and gene expression of cut flowers. The results revealed that E-0 treatment exhibits a higher postharvest quality of cut flowers than others. E-3 treatment significantly decreased the vase l ife of c ut r oses d ue t o an early f ailure o f water relations, such as larger stomatal size, higher transpiration, shorter time maintaining positive water balance, and the bacterial proliferation. E-0 treatment significantly decreased water stress, maintained leaf chlorophyll fluorescence ratios, and extended the vase life of cut roses. The decrease in water stress of E-0 treated flowers may increase the expression of Rh-PIP2;1 and Rh-TIP in rose petals, resulting in maintained cell turgor and increased flower diameter of cut flowers. These results recommend that a non-exposed harvest can be used for the harvest s tage to improve the postharvest quality of cut flowers. Understanding the relationship between air exposure time and water relations of cut flowers will significantly help in developing distribution systems for assuring cut roses quality.

In the Fourth Industrial Revolution, digitally effective automatic tools in agriculture are being devised to produce food quickly. Therefore, it is essential to develop a deep understanding of digital technology and its features for the consideration of new relationships, arranged skills, and cultural economics to attain the best value for the consumption of agricultural products. Farmers play a crucial role in replacing traditional agricultural methods with new technologies in their fields. Experienced farmers can draw on the Internet of Things (IoT) to benefit from smart technologies, such as gateway data stores in light, humid, and pH sensors, and improve all sensor data daily in big sectors starting from their smart farms. This report focuses on the relationship between smart farms and IoT with new network knowledge and smart devices to benefit agriculture. This report hopes to support the farmers who intend to draw on the most recent advances in IoT-based smart farming against the backdrop of evolving sensors, tackling current

We examined the efficacy of various pretreatment solutions on the postharvest longevity and quality of ‘Hessa’ cut spray roses (Rosa hybrida L.) to develop an inexpensive and innocuous preservative solution for the cut flower industry. Cut flowers were pretreated with 4 preservatives for 10 hours: Scutellaria baicalensis Georgi extract (SC) at 300 μL·L-1, hydrosol (H), S. baicalensis Georgi extract 300 μL·L-1 + sucrose 1% (SC + Suc), and H + sucrose 1% (H + Suc). The results showed that pretreatment with all the solutions except H + Suc improved the postharvest longevity and extended the longevity of cut rose flowers. We found that 300 μL·L-1 of SC was the most effective preservative, which significantly prolonged the postharvest longevity in cut flowers from 9.7 days (control) to 14.1 days. The beneficial effects of 300 μL·L-1 SC pretreatment were associated with inhibition of bacterial accumulation at the basal portion of cut stems, enhanced water uptake, improved fresh weight, and a positive water balance. SC also effectively maintained chlorophyll fluorescence ratios in the leaves and reduced water stress in cut roses. The results demonstrate the potential of SC as an alternative preservative in the cut rose flower industry.

American foulbrood (AFB) is caused by the bacterium Paenibacillus larvae, which is highly contagious and often lethal to honeybee broods. To control AFB, rapid diagnostic tools including those based on immunological methods are required. We produced several specific mouse monoclonal antibodies (MAbs) against P. larvae. Interestingly, a few of the MAbs were revealed to be an IgM-type antibody. To ascertain the effects of adjuvants on immunoglobulin isotype switching, BALB/c mice were immunized with various adjuvants, i.e., Freund's adjuvant (FA), Alum adjuvant, and AddaVax™ followed by the generation of hybridoma that secreted monoclonal antibodies to P. larvae. In the case of AddaVax™, all screened hybridoma clones secreted IgG-type MAbs, whereas hybridomas generated by Alum and FA secreted 91.25% (7/80) and 66.67% (11/33) respectively, IgG-type MAbs. Although the mechanism of incomplete immunoglobulin isotype switching associated with the P. larvae antigen needs further study, our results indicate that the applied adjuvants can have a significant effect on immunoglobulin isotype switching results.

South Korea has over 0.38 million of managed honey bee (Apis cerana) colonies before 2009 years ago, which produce the highest quantity of honey in the Korea; however, almost colony (90%) were collapsed by Korean Sacbrood Virus (KSBV) in South Korea. Korean Sacbrood Virus (KSBV) is the pathogen of A. cerana Sacbrood disease, which poses a serious threat to honeybee A. cerana, and tends to cause bee colony and even the whole apiary collapse. Colony collapse of A. cerana was first reported on the Pyeong-Chang of the South Korea in 2009. Several scientists and governments has been tried research for cure the sacbrood disease in A. cerana colony by medicines and management techniques. Unfortunately, The sacbrood disease dosen`t improve. So, we were developed a better breed of A. cerana for resistance of sacbrood virus by selection and then artificial insemination. A. cerana breeding technique was first successful applied with A. cerana in Korean. Queens was grafted from sacbrood resistance line and then it was growing in sacbrood disease colony that was survived 100%. Altogether selected 18 queens were artificially inseminated and 2,000 drones of A. cerana in Korea was used to evaluate amount of semen collection. We are select two scabrood resistance A. cerana line (R and H). R line be used for rearing the Queen. Drone was reared in H line colony. The RH hybrid were not infected sacbrood virus even spread sacbrood virus (2×106). RH colonies have very excellent hygienic behavior, brood, and sacbrood disease resistance activity.

Drosophila suzukii (Matsumura) is one of the most destructive pest of thin-skinned fruits such as blueberry, strawberry, raspberry, and cherry. We tested effect of fruit damage on the infestation of D. suzukii by using partially peeled blueberry fruits in assays of behavioral choice and development. In choice test, D. suzukii preferred partially peeled blueberry fruit than normal. But in development test, we did not found difference in adult emergence rate between normal and partially peeled blueberry fruit.