Attacin is a well-studied glycine-rich antibacterial protein in insect immune response, which has limitary antibacterial effect to some Gram-negative bacteria. A cDNA encoding the attacin gene was screened and isolated from the immunized larvae of the swallowtail butterfly, Papilio xuthus. The complete P. xuthus attacin cDNA is 949 nucleotides encoding a 250 amino acid precursor that contains a putative 18-residue signal peptide, a common 42-residue propeptide sequence and a presumed 190-residue mature protein with a theoretical mass of 19904.01 and a pI of 9.13. The putative mature protein of P. xuthus attacin showed 48%~52% and 24%~30% identity in amino acid sequences with that of lepidopteran and dipteran insects, respectively. The attacin transcript was induced at significant level after injection with bacterial lipopolysaccharide (LPS). Recombinant attacin was highly expressed in E. coli BL21 (DE3) cells by fusing with an N-terminal S-tag/thrombin cleavage site configuration protein to avoid the cell death during induction. The expressed fusion protein was purified by Ni-NTA immobilized metal affinity chromatography (IMAC). After desalting and cleavage with thrombin, the recombinant attacin was released and showed considerably antibacterial activity against Gram-negative bacteria, E. coli ML 35. Our results proved that this protein family with a potent antibacterial activity may play a role in the immune response of butterflies.

Hereditary dentin defects consists of dentin dysplasia(DD) and denti nogenesis imperfecta(Dr) ‘ The Dl associated with osteogenesis imperfecta has been classified as DI type 1. whereas isolated inherited defects have been categori zed as DI types II and III , However‘ whether DI type III should be considered a distinct phenotype 01' a variation of DI type 1I is debatable , Recent genetic findings have focused attention on the role of the dentin sialo phosphoprotein(DSPP) gene in the etiology of inherited defects of tooth dentin, We have identified novel mlltation( c,727G - > A, p,D243N) at the 243th codon of exon 4 of the DSPP gene in a Korean patient with DI type III The radiographic and histologic features of the patient revealed the classic phenotype of shell teeth These findings sllggest that DI type II and III are not separate diseases bllt rather the phenotypic variation 01' a s ingle disease

The use of natural paint for the application to walls and furnishings is now increasing to improve indoor air quality, thereby the natural paint could be a significant source of biogenic volatile organic compounds (BVOCs) in indoor environments. Recent studies have shown that gas-phase reactions between terpenes and ozone can generate sub-micron size particles and toxic volatile organic compounds such as aldehydes and ketones. In this research, we have studied the formation of particles and secondary organic compounds during the reaction of ozone with terpenes emitted from commercial natural paint. The paint applied onto stainless steel was dried and oxidized in a teflon chamber. Two monoterpenes (α- and β-pinenes) were identified by FTIR and GC/MS. Several tests were performed to evaluate the effects of ozone concentration on particle formation. Increased ozone levels significantly affect the increase of particle number concentration (monitored with SMPS), which results in the increase of particle counts ranging from 8,000 to 70,000 particles/㎤. Gas-phase products such as formaldehyde, acetaldehyde, acetone + acrolein, and propionaldehyde were identified during the terpene/ozone reactions by HPLC. These compounds are potential hazardous chemical compounds having harmful health effects to animals and plants. The results obtained from this study provide an insight on the adverse effect of eco-friendly natural product on indoor air quality (IAQ).

미국 9.11 사고 이후 테러는 과거에 비하여 다중이용시설 공격을 통한 불특정 다수의 공격이 증가하고 있다. 연이은 런던 폭탄테러, 파키스탄의 자폭 등은 사람들의 공포심 및 사회적 불안감을 증가시켰다. 최근 국내에서 다양한 국제행사가 개최되고 있어, 방사능테러 위협에 대비한 방사성물질의 국가 안보 의식이 증대되고 있다. 본 논문에서는 HotSpot Code를 사용하여 서로 상이한 기상조건에 따른 결과를 비교하였다. 국내에서 발생 가능한 테러 시나리오 작성 후, RDD(Radiological Dispersal Device) 및 더티밤에 사용될 가능성이 높은 선원을 조사하였다. 기상조건은 Pasquill-Gifford 안정도 등급에 따라 가장 안정된 조건의 F, 가장 불안정한 조건의 A를 선택하여 비교하였다. 시뮬레이션을 통한 A, F 등급 결괏값은 방사선학적 영향에 의해 시민들이 급성 영향으로 사망하는 경우는 없다고 판단하였다. 또한, 풍속 및 기상 안정도에 따라 방사능의 도달 정도가 서로 다르며, 기상 조건에 따라 방사능 희석정도가 서로 다름을 확인할 수 있다. 분석결과는 방사능테러 발생 시 초동 대응에 활용할 수 있을 것으로 예상된다.

The morphometric truss characteristics and classical dimensions of the marine medaka, Oryzias dancena, that might distinguish diploid and triploid fish were examined. Significant differences in all the classical and truss dimensions of the diploid and triploid fish were observed in both sexes (p<0.01). All the dimensions of the triploid fish were greater than those of the diploid fish. The triploid marine medaka shows sexual dimorphism in these characters, and the sexual dimorphism of the triploid marine medaka is similar to that of the diploid marine medaka. Thus, when their classical dimension and truss dimension was measured, the growth of triploid marine medaka is faster than that of the diploid fish, and it displays clear sexual dimorphism, with male fish having longer dorsal and anal fins than female fish.

The objective of this study was to determine the mitotic intervals (τ0) of two consecutive cell divisions and synchronous embryonic cleavage in grass puffer, Takifugu niphobles at different water temperatures (18, 20, 22, and 24℃). The color of the fertilized egg was light yellowish. The egg type was demersal and unadhesive. Egg weight was 0.09±0.002 mg. The sizes of unfertilized eggs were smaller than fertilized eggs in major axis and minor axis at 20℃ (p<0.05). The size of the fertilized egg of 18℃ water temperature group at the blastodisc stage was the smallest (p<0.05), but no significant differences were observed in the other water temperatures group except 18℃ water temperature group (p>0.05). The first cleavage stages at 18, 20, 22, and 24℃ were at 75, 90, 105, and 120 mins, respectively. As water temperature was increased, embryonic development and formation time of the first cleavage furrow were accelerated. There were negative correlation between τ0 and water temperature for grass puffer (Y=–1.225X+70.05, R2=0.988, n=10, where Y was τ0 and X was temperature). This study confirmed that successful hatching of grass puffer was related to water temperature. Chromosome manipulation will be helpful for this species using cleavage frequency and τ0.

This study was conducted with the aim of evaluating the antibiotic effects of leaves, stems, and roots in yacon (Smallanthus sonchifolius). Antibacterial activity of leaf extract by disk diffusion method with Bacillus subtilis and Escherichia coli respectively showed 13.3 and 13.75mm diameters of clear zone. There was no significant difference between the stems and leaves. The minimum inhibitory concentration of leaves’ heating and agitation extraction showed a restrain of strain at 1mg/ml, but the stems and root extract did not appear. Yacon is a functional antibacterial material, and methanol extraction is more effective than water. This research was to investigate the growth stage of collection has the most effective antibacterial effects. It has collected yacon's leaves from June to October, which is an appropriate time for collection right before reaping. Yacon leaf has antibacterial effects on Bacillus subtilis, Escherichia coli, Enterococcus faecium, and Salmonella enteritifis. There were no significant differences by the growth stage of collection. Leaves collected in July are high in phenol which helps in sulfating activity works well considering the high scavenging capability of DPPH. Leaves collected in September are high in total flavonoid.

Background : Insect pests damages are increasing on the field of Lycium chinense under eco-friendly organic management and especially, pest damage of Gelechiidae (Ilseopsis parki Povolny) is serious. Currently, various eco-friendly organic materials are registered, but insecticidal activity against Gelechiidae was not verified. Therefore, this experiment was carried out to select eco-friendly organic materials showing insecticidal activity in Gelechiidae. Methods and Results : To test insecticidal activity, the Gelechiidae lavas were collected from the field and Nine eco-friendly organic materials were collected from the market. method. The insecticidal activity assay method was, putting Gelechiidae lavas on the 90mm petridish then water-diluted eco-friendly organic materials were sprayed onto the laver. Result of insecticidal activity was observed with a microscope 10 minutes, 2 hours and 18 hours after the treatment. Microscopic observation results, insecticidal activity was shown only in 5 out of 9 materials. Conclusion : As a result, Insecticidal activity against Gelechiidae was not effective in all tested materials, but the 5 eco-friendly organic materials showed the same effect in repeated experiments. In this study, we selected 5 materials out of 9 eco-friendly organic materials against Gelechiidae, then future studies are planned to select the most suitable materials by field testing the 5 eco-friendly organic materials.

Background : Black Non-Woven Fabric Mulch Culture was knowned increased crop Yield and saved weeding labor in Liriope Pilatyphylla Wang et Tang. But to the removal and planting labor is more needed, So some famers are avoidance that culting method. Methods and Results : So this study was experimented in order to selecting optimun removal time in Liriope Pilatyphylla Wang et Tang mulch culture. Removal time were conventional practices (in April next yesr), September, October and November. In early, Plant length, Root length, Leaf number and number of plants was the long and many by the sooner removal time and also, dry weight was heavier. Black non-woven fabric removal labor was saved by the sooner removal time. The main event of weeds were Cyperus serotinus Rottb and Portulaca oleracea L. In harvest time, Plant length and plants of numbers was the longest and heavier at conventional practice (in April next year) and November removed. Tuberous root number was the more in September removal, Because, the tuber was tall and long. Total1y consideration of the including weeds shooting, weeding labor and Growth and development situation, Black non-woven fabric removal optimum time was September or Conventional practices (in April next year). Conclusion : Black non-woven fabric optimum removal time was the september . In this experiment, increased yield 9, income 16 percent than conventional practices (in April next year).

Background : Natural Mortality Vinyl much culture are increased crop yield and saved weeding workforce. But research is insufficient that of Liriope Pilatyphylla Wang et Tang. Black non-woven fabric mulch culture are increased crop yield and superior to occurrence of weeds, but to the removal and planting more labor needed, so, Farmers are avoidance using that. Methods and Results : So this study designed in order to selecting the best covering material. Using in this study, covering materials were Conventional practices (non covering), Natural mortality vinyl and black non-woven fabric. Soil temperature was continued highly after planting from in mid-may to in august a regular. among them natural mortality vinyl mulch was the highest. Natural mortality vinyl mulch was the fastest that humidity of soil change and Growth and development situation by maintaining proper temperature and humidity. Non mulching was little change in temperature and humidity and the slowest in the Growth and development situation. The main event of weeds were Cyperus serotinus Rottb and Portulaca oleracea L. Weeding labor was saved from 43 to 57 percent in the natural mortality vinyl and black non-woven fabric mulch. Plant length was the shortest in the conventional practices and Root length, Leaf number and number of plants were little changed. Yield was increased from 27 to 29 percent in the natural mortality vinyl and black non-woven fabric mulch than conventional practices. Result of comprehensive economic analysis including weeding labor and yield, the natural mortality vinyl mulch culture was income increased 92 percent than conventional practices. Conclusion : Natural mortality vinyl mulch culture were weeds shooting controled and increased yield 39, income 92 percent than conventional practices (non covering).

Background : The purpose of this study was to evaluate methods to high quality food ramie rice cake, thereby increasing farm income. This study investigated the effects of different Split Application and Adapted Fertilizer Culture for Stable Production with Edible Leaf in Ramie (Boehmeria nivea L.). Methods and Results : Method of fertilizer application were conducted under three condition compose to standard application (N-P-K-Compost applied at 27-9-27-1,500 ㎏․ 10 a−1), 25% increased application (N-P-K-Compost applied at 34-11-34-1,500 ㎏․10 a−1), 50% increased application (N-P-K-Compost applied at 41-14-41-1,500 ㎏․ 10 a−1) cultivation. Compost and fused phosphate applied at 100% of basal fertilizer in March 25. The split application level of standard application (N-K) applied at 50% of basal fertilizer in March 25, 50% of top dressing were five times application (N-K) in May 4, July 5, September 5, October 5, 20% respectively. Transplanting with Yeongkwang varieties in April 12 by growing pot seedling for 35 days in 2011. Planting density were spaced 60 ㎝ apart in rows 25 ㎝ apart with rice straw cutting covered open cultivation. Plot design randomized block 3 repetition. Conclusion : Organic matter, available phosphate, K2O, CaO, and MgO of soil increased in all plots with the addition of fertilizers culture, specifically in 25% and 50% increased application. Growth characteristics by 50% of top dressing were five times application (N-K) in May 4, July 5, September 5, October 5, compared to standard application (N-K) applied at 100% basal fertilizer, aerial part as a result were so many such amount of growth as number of branch and leaf per plant, etc., Length, width of leaf and leaf colorimetry of aboveground part growth increment rising highest. Green leaf yields compared to standard fertilizer 100% application (1,344 kg․ 10 a−1). 25% and 50% increased application (N-K) applied at 50% basal fertilizer, 50% of top dressing were five times application increased by 9%, 17%. respectively.

Optimum concentrations of anesthetic clove oil and anesthetic lidocaine-HCl were determined for a species of adult marine medaka, Oryzias dancena, over a range of salinity conditions, and investigated in a transport simulation experiment by analyzing various water and physiological parameters. Research indicated that the higher the concentration of anesthetic at each salinity, the shorter the anesthesia time at each salinity. At each concentration, fish were anesthetized slower at water salinities over 10 ppt (P<0.05). Anesthesia time at 10 ppt was faster than any other salinity. In 10 ppt salinity, the dissolved oxygen (DO) concentrations and respiratory frequencies of the clove-oil-administered groups decreased until 48 hours (P<0.05), whereas the NH4 + and CO2 concentrations increased until 48 hours (P<0.05). In same period, the DO, NH4 +, and CO2 concentrations and respiratory frequencies all decreased as the clove oil concentration increased (P<0.05). The trends in the DO, NH4 +, and CO2 concentrations and respiratory frequencies in the lidocaine-HCl-administered groups were similar to those in the clove-oil-administered groups. In conclusion, clove oil and lidocaine-HCl are effective anesthetics, improving the transportation of the marine medaka. The results from this study will contribute to safe laboratory handling of the marine medaka, which are commonly required by many research studies and experiments.

The marine medaka, Oryzias dancena is a suitable sample as a laboratory animal because it has a small size and clearly distinguishes between female and male. Data on the growth and maturity of the diploid and triploid sea cucurbit species suitable for laboratory animals are very useful for studying other species. Triploidy was induced in the marine medaka by cold shock treatment (0°C) of fertilized eggs for 45 min, applied two minutes after fertilization. The diploid and triploid male fish were larger than their female counterparts (P<0.05), and the concentrations of thyroid stimulating hormone (TSH) and thyroxine (T4) were higher in the induced triploids over 1 year (P<0.05). In both the diploid and tri-ploid groups the concentrations of TSH and T4 were higher in the male fish than in the females (P<0.05), while the testo-sterone and estradiol-17ß concentrations in the induced triploids were lower than in the diploids (P<0.05). The gonadosomatic index (GSI) of the triploid fish was lower than that for the diploids, and the GSI for females in each ploidy group were higher than that for the males. For both groups the GSI was highest at 4 months of age, and decreased thereafter to 12 months. Analysis of the gonads of one-year-old triploid fish suggested that the induction of triploidy probably causes sterility in this species; this effect was more apparent in females than in males.

Background : Angelica dahurica Bentham et Hooker, Angelica gigas Nakai, Ostericum koreanum Maximowicz and Peucedanum japonicum Thumberg　are a major medicinal plant in north Geungbuk province. Using medicinal plants are impotant it`s ingredient. Dry condition and stroage method are not standard manual. The ingredient variation of dry condition and stroage method were not researched. Methods and Results : Using plant material were cutivated on Gyongsangbukdo Bonghwa area. It were studied ingredient variation after dry and storage condition by HPLC methods. Major ingredient of Angelica gigas Nakai are decurusin, decurusinangelate. Heated air bulk dry get more decursin than natuarl dry and decurusinangelate of natural bulk dry was higher than heated air bulk dry. Major ingredient of Ostericum koreanum Maximowicz are imperatorin and isoimperatorin.. Imperatorin of Ostericum koreanum was highest peak on 50℃ heated-air dry after plastic bag sorage and isoimperatorin was highest peak on 40℃ heated-air dry after mountain cultivation. Imperatorin is a major ingredient Angelica dahurica Bentham et Hooker. Heated air bulk dry get more decursin and decursinangelate than natuarl dry and small heated-air dry. Peucedanol-7o_glucoside is a major ingredient Peucedanum japonicum Thumberg. Natural bulk dry get more peucedanol-7o_glucoside than heated-air bulk dry. Conclusion : Ingredient of Angelica dahurica Bentham et Hooker, Angelica gigas Nakai, Ostericum koreanum Maximowicz are different under various cutivation, drying method, storage. Diffent Ingedients of Angelica gigas Nakai, Ostericum koreanum Maximowicz were not accord it’s optical conditon.

The optimum concentrations of clove oil as an anesthetic for olive flounder (Paralichthys olivaceus) and the stress response of the fish to clove oil anesthesia were determined over a range of water temperatures, and investigated in a simulated transport experiment using analysis of various water and physiological parameters. While the time for induction of anesthesia decreased significantly as both the concentration of clove oil and water temperature increased, the recovery time increased significantly (P<0.05). The plasma cortisol concentration in fish at each temperature increased significantly up to 12 h following exposure (P<0.05), then decreased to 48 h (P<0.05). The DO dissolved oxygen concentrations, pH values, and the fish respiratory frequencies decreased over 6 h following exposure to clove oil in all experimental groups (P<0.05), whereas the NH4 + and CO2concentrations in all experimental groups increased up to 6 h (P<0.05). The pH values and DO concentrations increased with increasing clove oil concentration (P<0.05) in the 6 h following exposure, and the CO2 and NH4 + concentrations and the respiratory frequencies decreased with increasing clove oil concentration (P<0.05). The results of this experiment suggest that clove oil reduced the metabolic activity of olive flounder, thus reducing NH4 + excretion and O2 consumption. In conclusion, clove oil appears to be a cost-effective and efficient anesthetic that is safe for use and non-toxic to the fish and users. Its use provides the potential for improved transportation of olive flounder.

Background : Curcuma longa L., in the family Zingiberaceae, is distributed in tropical and/or sub-tropical regions mainly in India and China. This species is commonly called turmeric, powder is used as medicinal herbs and/or flavor enhancer. It has been cultivated in southern region mainly Jindo. However, it might be possible to extend cultivation regions due to rise in average temperature. In order to select superior lines based on agronomic characteristics, we analyzed multivariate and estimated selection effects from C. longa germplasm. Methods and Results : The C. longa germplasm were cultivated in an experimental field located in Eumseong, NIHHS, RDA. The harvested roots were investigated in agronomic characteristics included in yield and then considered its relationship among the 9 germplasm by multivariate analysis method. Results from principal component analysis (PCoA) showed that it represented 70.00% and 80.44% accumulated explanation from four and five principal compounds (PC). PCoA was conducted from 9 agronomic characteristics and then correlation coefficient has been showed by analysis between each main component value and agronomic characteristics. Value of the first PC was 2.25, 24.96% explanation of total dispersion, plant height, number of rootlet and weight of rootlet were correlated with a somewhat higher level as 0.41, 0.43 and 0.52. Value of the fifth PC was 0.94, 10.43% explanation of total dispersion, the number of shoots was correlated with a higher level as 0.87. Selection effects with outstanding candidate lines including higher lines were estimated at 126.13% in yield. Conclusion : These data on multivariate based on agronomic characteristics will be give us invaluable breeding information by selection of superior lines.

Background : An important feature of the nutrient solution is that they affect not only the growth but also quality of crops by changing nutrient uptake, especially due to changes of EC in nutrient solution. This study was carried out to investigate effect of EC in nutrient solution on growth and ginsenoside of ginseng. Methods and Results : EC in nutrient solution was controlled with 0.68, 0.84, 1.23, 1.41 dS/m. The root weight of ginseng treated by low EC levels in nutrient solution was higher during the initial of growth. However, the higher EC levels, the more increased the change rate of root weight from the initial to the middle of growth. The highest amount of ginsenoside was changed by growth period. Although the total amount of ginsenoside in root is highest treated by EC 0.68 dS/m at 45 days after treatment. the total amount of ginsenoside in root is highest treated by EC 1.23 dS/m at 135 days after treatment. Conclusions : EC in nutrient solution should to be controlled depending on the stage of growth and the part of use, i.e. root and leaves, when ginseng is cultivated through nutri-culture.

Background : Boron (B) is an essential element required for the growth of plant. It has a narrow range of optimal concentration from minimum to maximum thresholds than other micro-elements. The study was carried out to investigate to the influence of B excess concentrations on physiological disorder of leaf, growth and mineral concentration of ginseng to obtain basic information for physiological disorder diagnose. Methods and Results : The ginseng cultivar ‘Gumpoong’ was cultivated by hydroponic system for 2 months. The toxicity symptoms which ginseng leaves were curved downwardly and induced to chlorosis after beginning to dry the edge appeared on leaves more than 30 ppm compared to the control(3 ppm). The growth of ginseng was more decreased with higher B concentration. Mn uptake was also decreased as B concentration increased. It was found that B excess hindered the growth of ginseng and was reversely related to Mn uptake. Conclusions : More than B 30 ppm can negatively affect growth and mineral uptake. Consequently, B excess can occur physiological disorder of ginseng.