As part of the KOPIA Paraguay Center rice pilot villages project, conducted from July 1, 2021, to the end of June 2024, this study examined the average yield and value of paddy rice harvested between January and April 2023 in five local rice pilot villages in Paraguay: Santa Rosa, Eusebio Ayala, Coronel Bogado, Santa Maria, and Yaguaron. The CEA-5K-PUNTA variety was planted in late August 2022 and subsequently harvested from January to April 2023. The farmers in the pilot villages received information on common pests, diseases, and weeds, including instructions on timing and the number of applications required for their control. They also received technical guidance on water management and weeding methods. The study found that the average yield per hectare in the pilot villages for the CEA-5K PUNTA variety in the 2022/2023 growing season was 794.50 kg/10a, compared to 477.17 kg/10a for the conventional variety IRGA 424 in the 2019/2020 season, which preceded the project. This indicates a 66.5% increase in yield per hectare for the pilot village farmers compared to the pre-project period (2020/2021). The analysis of paddy rice production value in the pilot villages, using the Difference in Differences method, revealed a 65.1% increase. Moreover, a financial analysis was conducted based on the addition of agricultural inputs to the paddy fields during the period from the end of August 2022 to January-April 2023. The analysis showed that the total cost was US$597.25 per hectare, while the gross income was US$1,685.3 per hectare. As a result, the net profit per hectare amounted to US$1,088.05.

Zooplankton biomass is essential for understanding the quantitative structure of lake food webs and for the functional assessment of biotic interactions. In this study, we aimed to propose a biomass (dry weight) estimation method using the body length of cyclopoid copepods. These copepods play an important role as omnivores in lake zooplankton communities and contribute significantly to biomass. We validated several previously proposed estimation equations against direct measurements and compared the suitability of prosomal length versus total length of copepods to suggest a more appropriate estimation equation. After comparing the regression analysis results of various candidate equations with the actual values measured on a microbalance-using the coefficient of variation, mean absolute error, and coefficient of determination-it was determined that the Total Length-DW exponential regression equation [W=0.7775×e2.0183L; W (μg), L (mm)] could be used to calculate biomass with higher accuracy. However, considering practical issues such as the morphological similarity between species and genera of copepods and the limitations of classifying copepodid stages, we derived a general regression equation for the pooled copepod community rather than a species-specific regression equation.

With the advent of the 4th Industrial Revolution, changes in the market environment and employment environment are accelerating due to smart technological innovation, and securing professional manpower and developing human resources for domestic small and medium-sized enterprises is becoming very important. Recently, most of the domestic small and medium-sized enterprises are experiencing hiring difficulties, and the development and training of human resources to overcome this is still lacking in systemization, despite much support from the government. This reflects the reality that it is not easy to invest training costs and time to adapt new employees to small and medium-sized businesses. Based on these problems, the work-study parallel project was introduced to cultivate practical talent in small and medium-sized businesses. Work-study parallel training is carried out in the form of mentoring between corporate field teachers and learning workers in actual workplaces, and even if the training is the same, there are differences depending on the learner's attitude, learning motivation, and training achievement. Ego state is a theory that can identify personality types and has the advantage of being able to understand and acknowledge oneself and others and intentionally improve positive factors to induce optimized interpersonal relationships. Accordingly, the purpose of this study is to analyze the attitudes of learning workers, who are the actual subjects for improving the performance of work-study parallel projects and establishing a stable settlement within the company, based on their ego status. Through this study, we aim to understand the impact of the personality type of learning workers on training performance and to suggest ways to improve training performance through work-study parallelism.

This study evaluated the virucidal efficacy of a chlorine dioxide (ClO2) gas-generating fumigation disinfectant consisting of sodium chlorate solution (25% sodium chlorate) and reaction solution against avian influenza virus (AIV). After AIV suspensions had been deposited on stainless steel carriers, the 9 dried carriers were exposed to the fumigant (sodium chlorate solution: 8.5, 17, 34, 50, and 100 mL) in a 25-m³ test room for 2, 3, and 4 h, respectively. Thereafter, all carriers were submerged in a neutralizing solution (20% fetal bovine serum) to scrape off the surviving viruses, and the respective suspensions were diluted. Each diluent was inoculated into the allantoic membrane of five 10-day-old embryos. After incubation for 5 days at 37℃, AIV viability in the collected allantoic fluids was examined, and the egg infectious dose 50 (EID50) was calculated. When the carrier was exposed to ClO2 gas generated from reacting 34 mL of the fumigant for 3 h, the AIV titer reduced by more than 104.0 EID50/carrier compared to the control, which was exposed to the fumigant without inoculation of AIV suspension. In addition, the control was non-toxic to the embryos.

Probiotic lactic acid bacteria are live microorganisms that provide health benefits when administered in adequate amounts and may exhibit antiproliferative effects on various cancer cell lines, including colon cancer. This study investigates the cytotoxic effects of three Lactobacillus strains - Limosilactobacillus (L.) reuteri VA 102, Ligilactobacillus (L.) animalis VA 105, and Limosilactobacillus (L.) reuteri KCTC 3594 (ATCC 23272) - on mouse colon carcinoma cells (CT-26). Live cells, heat-killed cells, and cell-free supernatant (CFS) of Lactobacillus sp. were prepared and used to treat CT-26 cells at different concentrations. The cytotoxic effect was assessed using the MTT assay. The results indicated that the CFS of all strains significantly reduced the viability of CT-26 cells in a dose-dependent manner, with the VA 102 strain showing the most pronounced effect. Heat-killed cells of L. reuteri VA 102 and L. reuteri KCTC 3594 (ATCC 23272) also reduced cell viability. These findings suggest the potential anticancer properties of these Lactobacillus strains and indicate that CFS and heat-killed cells may offer a safer and more effective alternative to live bacteria for therapeutic applications. Our study contributes to the understanding of the potential of Lactobacillus strains, particularly L. reuteri VA 102, L. reuteri KCTC 3594 (ATCC 23272), and L. animalis VA 105, as possible candidates for cancer treatment and control.

Pathogenic Escherichia coli strains are known to cause edema disease (ED) and postweaning diarrhea (PWD) in piglets. Although the exact mechanisms of pathogenicity that lead to ED-PWD remain to be elucidated, we investigated whether another E. coli adhesin, the plasmid-encoded adhesin involved in diffuse adherence (AIDA) might also be present in ED-PWD-causing E. coli isolates. It was showed that E. coli strains attach to HEp-2 cell assay in three different patterns. Twenty-two isolates were from faeces of preweaned pigs and 36 isolates were from faeces of postweaned pigs. The base sequences for specific oligonucleotide primers of PCR used in this study were constructed based on the regions of conserved sequences between forward (5′-3′) ACAGTATCATATGGAGCCA and reverse (5′-3′) TGTGCGCCAGAACTATTA. Product size was 585 base pairs. A total of 58 AIDA-positive E. coli were used for cell adherence pattern analysis of HEp-2 cell assay. Forty three isolates showed three distinct patterns that were localized adherence (LA), diffuse adherence (DA) and aggregative adherence (AA) patterns respectively. But fifteen isolates were nonadherent. LA (14 isolates) pattern was the most common, followed by DA (13 isolates) and AA (9 isolates) pattern. And three isolates showed a combination of the LA and AA patterns and 4 isolates showed a combination of the DA and AA patterns. Escherichia coli strains that cause nonbloody diarrhea in infants are known to present three distinct patterns of adherence to epithelial cells, namely, localized (LA), diffuse (DA), and aggregative (AA) adherence.

Nursing research in veterinary hospitals is critical to the work of veterinary nursing and is necessary for continued advancements that promote optimal nursing care. Since the work of veterinary nursing involves animals with whom communication is difficult, the level of work performance can vary greatly depending on the experience, knowledge, and abilities of each animal nurse. In addition, veterinary nursing might establish a successful work environment through collaboration with veterinarians, and smooth work cooperation and communication among hospital members are direct factors in improving the work performance of animal hospitals. Because the work of nursing shows significant differences in performance depending on the individual, much research is being conducted to develop tools to measure work performance in the veterinary nursing field of medical assistants. In the present study, we attempted to develop a work performance measurement tool that is useful and suitable for veterinary nursing using the Delphi method. As a result of this study, an 18-item questionnaire was developed to measure the work performance of veterinary nursing, and these evaluation items were found to have excellent suitability in terms of content and construct validity. The evaluation scale of work performance of animal nurses developed through this study is believed to be useful in evaluating work performance in terms of work performance ability, attitude, and work performance relationship. Additionally, it is considered that the results of this study can be actively used to understand and develop the work relationships of veterinary nursing.

The objective of this study was to detect and identify the bee mites that were related to the colony collapse disorder of the western species of honey bee. From July 6, 2023 to August 2, 2023, total 367 bee larvaes and 243 adults bee were observed by 100-fold and 200-fold magnification microscopy. As a result of direct visual and microscopic examination on honeybee bodies, larvae and pupae, a total of 2,688 bee mites were found. Of their bee mites, it were 9 of Tropilaelaps clareae, 130 of Varroa jacobsoni, and 2,549 of Varroa destructor, respectively. Tropilaelaps clareae showed 7 females, 1 male, and 1 larva, Varroa jacobsoni had 94 females, 5 males, and 31 larvae, and Varroa destructor had 2,162 females, 346 males, and 41 larvae, respectively. In order to reduce the damage of the apiary caused by bee mites, it is mainly dependent on pharmaceutical control, continuously.

Objective: This study aimed to translate and validate the Motivation to Change Lifestyle and Health Behaviors for Dementia Risk Reduction (MCLHB-DRR) scale among middle-aged and older Korean adults. Methods: This study included a sample of Korean adults aged ≥ 50 years recruited from an online panel. Content and construct validity were examined to determine the scale’s validity. Internal consistency and test-retest reliability were examined to determine its reliability. Results: A total of 270 participants completed the questionnaire. Content validity was determined by professors and researchers of occupational therapy. One item was deleted during the content validity assessment. The confirmatory factor analysis results showed a good model fit (χ 2/degree of freedom = 1.845, comparative fit index = 0.939, Tucker-Lewis index = 0.929, root mean square error of approximation = 0.056). Cronbach’s alpha values were 0.821 - 0.900, indicating good internal consistency. The test and retest scores did not differ significantly. Conclusion: The Korean version of the MCLHB-DRR scale has validity and reliability for use among the Korean population, to assess beliefs and attitudes toward lifestyle change to reduce the risk of dementia.

Helicobacter pylori are known as a causative agent of gastritis, gastric duodenum and peptic ulcer, and gastric cancer, and multiple drug use is associated with various side effects in patients. The discovery of antibacterial substances against H. pylori from Korean resource plants is an important substitute for antibiotics. 52 species of Korean resource plants were collected and extracted with 50% ethanol, and antibacterial activity against H. pylori was measured using the disk diffusion method. The toxicity of plant extracts to human gastric adenocarcinoma(AGS) cells was measured by MTT assay, and the level of IL-8 secreted when gastric epithelial cells were inoculated with H. pylori was measured. As a result of measuring the antibacterial activity of H. pylori, antibacterial activity was confirmed in 38 plant extracts. The plant species with the strongest antibacterial activity were Chrysanthemum indicum, Rheum rhabarbarum, Patrinia scabiosaefolia and Petasites japonicus. C. indicum was not cytotoxic to H. pyroli-infected AGS cells and showed anti-inflammatory effects. This study's results can be used to develop healthy, functional foods and medical materials.

Caprine cryptosporidiosis mainly occurs in young goats, with morbidity rates of 80%–100% and mortality over 50% in goat kids. However, limited research has been conducted on the impact of Cryptosporidium parvum, a diarrhea-causing pathogen, on the intestinal microbiota of goat kids. In this study, 16S rRNA-based metataxonomic analysis was performed to compare the microbial diversity and abundance of the gut microbiota between C. parvum-infected and uninfected goat kids. In total, 12 goat fecal samples were collected, including seven naturally C. parvum-infected and five uninfected goats from Chungcheongbuk-do, Korea. After amplification of the V3–V4 hypervariable region of the bacterial 16S rRNA, high-throughput sequencing was performed. The results showed differences in the microbial composition between C. parvum-infected and uninfected groups based on beta diversity. Firmicutes and Bacteroidetes were the most dominant phyla in both groups. However, no significant difference was observed in the Bacteroidetes/Firmicutes ratio between the two groups. Compared with the uninfected group, the C. parvum-infected group showed significantly higher abundances of Tyzzerella nexillis, Lactobacillus johnsonii, Butyricicoccus pullicaecorum, Enterococcus raffinosus, Enterococcus faecalis, and Negativicoccus massiliensis, and significantly reduced abundances of Aerococcus vaginalis, Faecalicoccus pleomorphus, Oribacterium parvum, and Coprococcus comes. These findings indicate that C. parvum infection, which is associated with diarrhea in neonatal goats, induces alterations in the caprine gut microbiota.

Fluorescent bacteria were isolated from sporocarps that browned into various mushrooms during survey at places of the production in Korea. We examined the pathogenicity, biodiversity, and genetic characteristics of the 19 strains identified as Pseudomonas tolaasii by sequence analysis of 16S rRNA and White Line Assay. The results emphasize the importance of rpoB gene system, fatty acid profiles, specific and sensitive PCR assays, and lipopeptide detection for the identification of P. tolaasii. As a result of these various analyses, 17 strains (CHM03~CHM19) were identified as P. tolaasii. The phylogenetic analysis based on the 16S rRNA gene showed that all strains were clustered closest to P. tolaasii lineage, two strains (CHM01, CHM02) were not identified as P. tolaasii and have completely different genetic characteristics as a result of fatty acids profile, specific and sensitive PCR, lipopetide detection, rpoB sequence and REP-PCR analysis. Pathogenicity tests showed 17 strains produce severe brown discolouration symptoms to button mushrooms and watersoaking of sporophore tissue within three days after inoculation. But two strains did not produce discolouration symptoms. Therefore, these two strains will be further investigated for correct species identification by different biological and molecular characteristics.

Electric-propulsion systems for ships, also known as electric propulsion devices, represent the current direction of development for maritime power. Issues concerning the environment and fuel economy have compelled the maritime transport sector to seek solutions that reduce emissions and improve fuel efficiency. In this process, power electronics technology plays a significant role in the propulsion systems of ships. Selecting an efficient battery system is of great importance for enhancing the cruising range of yachts and minimizing environmental impact. The battery model is crucial for revealing the working principles of batteries, and it is extremely critical for the application and development of battery technology. The Battery Management System (BMS) serves a crucial regulatory function, optimizing both the safety and performance of battery cells. Central to its operation is the precise estimation of the battery's State of Charge (SOC), a process dependent on an exacting battery model. This system not only enhances longevity and reliability but also ensures that energy storage solutions meet high standards of efficacy. This study focused on testing the impedance characteristics of lithium-sulfur batteries (LSB) at various SOC points and establishing first- and second-order RC equivalent circuit models. The model parameters were identified through experimental data. Subsequently, a simulation platform was constructed using MATLAB/Simulink to simulate the behavior of LSB under a constant current discharge condition. The simulation results showed that the second-order RC model had significantly lower errors than the first-order model, demonstrating higher accuracy. These achievements can provide technical support for the research of energy storage systems in the green aviation and maritime industries.

This study was conducted to collect the patents of microbiome-based treatment technology for pets. An electronic search for microbiome or probiotics in brain nervous system disease was studied using the WINTELIPS database. Patent Cooperation Treaty of Korea, Japan, the EU, the US, and China that were registered by October 31, 2022 were selected in this study. A total of 206 patents were included for final analysis. Since 2016, patent activity has shown an explosive increase in recent years. China is the leading market in this technology field, and Korea has the second-highest market share. To provide the groundwork for the next research and development, we examined the industrial trend of microbiome for brain nervous system diseases in this study using an analysis of patents that have been applied for and registered up to this point. Looking at the overall patent trends by year in the technology field related to treating of brain and nervous system diseases using the microbiome, there was a tendency to repeat increasing and decreasing trends. However, considering 2021 and 2022, which have undisclosed sections, it can be seen that patent activity has tended to increase explosively in recent years, starting in 2016. If related studies use the patent analysis data constructed in this way strategically, it is expected that it will lead to patent registration and the development of new products, ultimately contributing to the revitalization of the companion animal industry.

최근 국가뿐만 아니라 도시에서도 자발적 지역 검토(Voluntary Local Review, 이하 VLR)를 실시하고 이를 토대로 보고서를 발표하는 지방자치단체가 늘고 있다. 도시 수준의 SDGs 조치는 새로운 도시 의제, 기후 변화에 대한 파리 협정, 센다이 재해 감소 프레임워크, 생물다양성에 대한 아이치 목표와 같은 다른 국제적 약속과 밀접하게 연관되어 있다. 이 논문은 지방정부의 VLR 방향을 제언하는 것을 목적으로 세계 여러 도시의 2030 의제 실행 보고의 구체적인 프로세스에 대해 논의한다. VLR 프로세스는 지자체와 지역 사회 문제를 해결하기 위한 맞춤형 접근 방식, 지역 기술을 강화하기 위한 역량 강화, 하위 국가 수준의 데이터 환경 개발, 학계, 민간 부문 또는 지역 사회와 같은 정부 및 비정부 이해관계자의 다양한 영역과의 오래 지속되는 파트너십 구축이 포함되어 있다. 본 연구에서는 기존 VLR의 분석을 통해 식별된 VLR 프로 세스의 각 단계 내 주요 구성 요소에 대해 논의한다. 본 연구는 VLR 개발 프로세스의 여러 단계를 개략적 으로 설명하고 지침에서 각 단계의 주요 구성 요소에 대해 살펴보고 이것이 가진 의미를 검토한다.

Diabetes, a chronic hyperglycemic condition, is caused by insufficient insulin secretion or functional impairment. Long-term inadequate regulation of blood glucose levels or hyperglycemia can lead to various complications, such as retinopathy, nephropathy, and cardiovascular disease. Recent studies have explored the molecular mechanisms linking diabetes to bone loss and an increased susceptibility to fractures. This study reviews the characteristics and molecular mechanisms of diabetes-induced bone disease. Depending on the type of diabetes, changes in bone tissue vary. The molecular mechanisms responsible for bone loss in diabetes include the accumulation of advanced glycation end products (AGEs), upregulation of inflammatory cytokines, induction of oxidative stress, and deficiencies in insulin/IGF-1. In diabetes, alveolar bone loss results from complex interactions involving oral bacterial infections, host responses, and hyperglycemic stress in periodontal tissues. Therapeutic strategies for diabetes-induced bone loss may include blocking the AGEs signaling pathway, decreasing inflammatory cytokine activity, inhibiting reactive oxygen species generation and activity, and controlling glucose levels; however, further research is warranted.

고주파 흡수제(Accusorb MRI)를 이용한 자기공명영상 검사 중 화재가 발생했다. 다행히 환자는 화상을 입지는 않았 지만 스트랩과 4채널 코일의 케이블, Accusorb MRI가 녹아서 탄 흔적이 발견되었다. 자기공명영상 검사에 사용되 는 도구의 안전 지침을 준수해야 하지만 이를 지키지 않으면 화상, 열상, 장비 손상의 위험이 있다. 사고를 방지하기 위해서는 철저한 사전 점검을 해야 하고 모든 기기, 물품들이 자기공명영상 검사 시 사용 가능한 제품인지 확인하고 사용법을 정확하게 숙지하여야 한다.

Rehmannia glutinosa root and Saururus chinensis plant have been widely used in natural traditional medicines. This study was conducted to investigate the effect of the content of the main components of R. glutinosa root and S. chinensis plant by extraction method. The results of comparative analysis of extraction yield, total flavonoid, and polyphenol content by extraction method indicated that extraction yield had the best performance with hot water extraction for R. glutinosa and ultrasound-assisted extraction for S. chinensis. The total flavonoid and polyphenol content had the best performance with maceration extraction for R. glutinosa and ultrasound-assisted extraction for S. chinensis. ABTS and DPPH activity was excellent with maceration extraction for both R. glutinosa and S. chinensis. The analysis of the main components showed that maceration extraction was most effective for both S. chinensis and R. glutinosa. Specifically, maceration extraction of R. glutinosa yielded 1.5 times more than conventional ultrasound-assisted extraction.

Meristem culture (MC) is a technique for producing virus-free garlic plants with high vigor and productivity. We assessed the changes in the agronomic traits of “Namdo” garlic over several generations after the cultivation of MC-induced bulbils. We examined the plant height, leaf sheath length and diameter, leaf number, bulb weight and diameter, clove number, and bulb size distribution. Compared with that of the control, bulb weights of the first-generation bulbils cultivated for three and two years and the second-generation bulbils cultivated for one year increased by 8.7–27.2, 13.9–30.4, and 36.6–46.9%, respectively. In three and two-year cultivation of the firstgeneration bulbils and one-year cultivation of the second-generation bulbils, the proportions of extra-large-sized bulb weight in meristem-cultured plants were 16.2–38.6, 24.0–35.8, and 27.1–51.1%, respectively, whereas that of the control was 7.6%. Thus, the first-generation bulbils can be cultivated for three years to renew the seed bulbs while maintaining productivity.

Hydraulic turbines can convert tidal current energy into electric energy, and the addition of a deflector cover to the turbine can improve the efficiency of the turbine's energy harvesting. The angle of the inlet section and the angle of the outlet section of the deflector will further affect the final energy-acquisition efficiency.A threedimensional numerical model for turbine flow field analysis is established, and the RNG k-ε turbulence model is selected by CFD method, and the best angles of inlet section and outlet section are analysed by the method of sliding mesh to obtain the best angle of inlet section and outlet section separately, and then three groups of angles are selected near the best angle of inlet section and outlet section to make orthogonal comparisons. The energy acquisition efficiency of the turbine is calculated at different angles of the inlet and outlet sections of the deflector, and the turbine streamline distribution, velocity and pressure maps are analysed with and without the deflector.The study shows that the deflector can play the role of convergence of the downstream flow, which can improve the efficiency of the turbine energy acquisition, and the maximum energy acquisition efficiency is at the inlet angle of 29° and the outlet angle of 40 °, and the maximum energy acquisition efficiency can be improved by about 32 percent.