미국 9.11 사고 이후 테러는 과거에 비하여 다중이용시설 공격을 통한 불특정 다수의 공격이 증가하고 있다. 연이은 런던 폭탄테러, 파키스탄의 자폭 등은 사람들의 공포심 및 사회적 불안감을 증가시켰다. 최근 국내에서 다양한 국제행사가 개최되고 있어, 방사능테러 위협에 대비한 방사성물질의 국가 안보 의식이 증대되고 있다. 본 논문에서는 HotSpot Code를 사용하여 서로 상이한 기상조건에 따른 결과를 비교하였다. 국내에서 발생 가능한 테러 시나리오 작성 후, RDD(Radiological Dispersal Device) 및 더티밤에 사용될 가능성이 높은 선원을 조사하였다. 기상조건은 Pasquill-Gifford 안정도 등급에 따라 가장 안정된 조건의 F, 가장 불안정한 조건의 A를 선택하여 비교하였다. 시뮬레이션을 통한 A, F 등급 결괏값은 방사선학적 영향에 의해 시민들이 급성 영향으로 사망하는 경우는 없다고 판단하였다. 또한, 풍속 및 기상 안정도에 따라 방사능의 도달 정도가 서로 다르며, 기상 조건에 따라 방사능 희석정도가 서로 다름을 확인할 수 있다. 분석결과는 방사능테러 발생 시 초동 대응에 활용할 수 있을 것으로 예상된다.

유방촬영장치(mammography equipment, ME)을 이용한 유방검사(mammography, MG)의 경우 유방암의 조기진단을 위해 가장 일반적으로 시행되고 있는 검사방법으로 유방 내 섬유화 조직, 미소석회화 및 종양덩어리의 발견과 진단은 유방촬영장치의 품질관리(quality management, QM)에 의해 크게 영향을 받게 된다. 특히, 유방검사에서 품질관리란 장치와 연관된 문제점들이 임상영상에서 영상의 질(image quality, IQ) 저하로 진단영역 축소를 초래할 수 있는 발생가능한 모든 문제점을 사전에 파악하여 교정함으로써 항상 일정 수준의 영상의 질을 유지하고 영상을 획득할 수 있게 하는 행위를 의미한다. 이에 본 연구진은 유방촬영장치를 이용한 유방검사의 품질관리에 대한 일반적인 내용을 요약하여 보고 한다.

To improve survival rates of vitrified pig oocytes, the treatment of cytoskeletal stabilizer on an appropriate time is one of the possible approaches. However, the exact treatment timing and effect of cytoskeletal stabilizer such as cytochalasin B (CB) is not well known during oocyte vitrification procedures. Thus, the present study was conducted to determine optimal treatment timing of CB during vitrification and warming procedures. In experiment 1, the survival rates of the post-warming pig oocytes were analyzed by fluorescein diacetate (FDA) assays with 4 classifications. In results, post-warming oocytes showed significantly (p<0.05) decreased number of alive oocytes (31.8% vs. 86.4%) compared to fresh control. In detail, the significant difference (p<0.05) was found only in strong fluorescence (18.2% vs. 70.5%) not in intermediate fluorescence groups (13.6% vs. 15.9%). In experiment 2, CB was treated before (CB-Vitri) and after (Vitri-CB) vitrification. In results, group of Vitri-CB showed significantly (p<0.05) higher (91.6%) survival rates compared to group of CB-Vitri (83.7%), significantly (p<0.05) and comparable with group of Vitri Control (88.7%) by morphological inspection. In FDA assay results, group of Vitri-CB showed significantly (p<0.05) higher (44.2%) survival rates compared to groups of CB-Vitri (36.7%) and Vitri Control (35.1%). In conclusion, the increased survival rates of post-warming pig oocyte treated with Vitri-CB method are firstly described here. The main finding of present study is that the CB treatment during recovery could be helpful to refresh the post-warming pig oocyte resulting its improved survival rates.

The objective of this study was to determine the mitotic intervals (τ0) of two consecutive cell divisions and synchronous embryonic cleavage in grass puffer, Takifugu niphobles at different water temperatures (18, 20, 22, and 24℃). The color of the fertilized egg was light yellowish. The egg type was demersal and unadhesive. Egg weight was 0.09±0.002 mg. The sizes of unfertilized eggs were smaller than fertilized eggs in major axis and minor axis at 20℃ (p<0.05). The size of the fertilized egg of 18℃ water temperature group at the blastodisc stage was the smallest (p<0.05), but no significant differences were observed in the other water temperatures group except 18℃ water temperature group (p>0.05). The first cleavage stages at 18, 20, 22, and 24℃ were at 75, 90, 105, and 120 mins, respectively. As water temperature was increased, embryonic development and formation time of the first cleavage furrow were accelerated. There were negative correlation between τ0 and water temperature for grass puffer (Y=–1.225X+70.05, R2=0.988, n=10, where Y was τ0 and X was temperature). This study confirmed that successful hatching of grass puffer was related to water temperature. Chromosome manipulation will be helpful for this species using cleavage frequency and τ0.

In the field of reproductive medicine, assessment of sperm motility is a key factor for achieving successful artificial insemination, in vitro fertilization, or intracellular sperm injection. In this study, the motility of boar sperms was estimated using real-time imaging via confocal microscopy. To confirm this confocal imaging method, flagellar beats and whiplash- like movement angles were compared between fresh and low-temperature-preserved (17℃ for 24 h) porcine sperms. Low-temperature preservation reduced the number of flagellar beats from 11.0±2.3 beats/s (fresh sperm) to 5.7±1.8 beats/s and increased the flagellar bending angle from 19.8°±13.8° (fresh) to 30.6°±15.6°. These data suggest that sperm activity can be assessed using confocal microscopy. The observed motility patterns could be used to develop a sperm evaluation index and automated confocal microscopic sperm motility analysis techniques.

Background : Insect pests damages are increasing on the field of Lycium chinense under eco-friendly organic management and especially, pest damage of Gelechiidae (Ilseopsis parki Povolny) is serious. Currently, various eco-friendly organic materials are registered, but insecticidal activity against Gelechiidae was not verified. Therefore, this experiment was carried out to select eco-friendly organic materials showing insecticidal activity in Gelechiidae. Methods and Results : To test insecticidal activity, the Gelechiidae lavas were collected from the field and Nine eco-friendly organic materials were collected from the market. method. The insecticidal activity assay method was, putting Gelechiidae lavas on the 90mm petridish then water-diluted eco-friendly organic materials were sprayed onto the laver. Result of insecticidal activity was observed with a microscope 10 minutes, 2 hours and 18 hours after the treatment. Microscopic observation results, insecticidal activity was shown only in 5 out of 9 materials. Conclusion : As a result, Insecticidal activity against Gelechiidae was not effective in all tested materials, but the 5 eco-friendly organic materials showed the same effect in repeated experiments. In this study, we selected 5 materials out of 9 eco-friendly organic materials against Gelechiidae, then future studies are planned to select the most suitable materials by field testing the 5 eco-friendly organic materials.

Background : Recently, hair loss, which has been regarded as a mere means of middle-aged men due to stress and environmental pollution. The market for hair loss in Korea is about four trillion won and it is growing continuously. It is mainly made by mixing natural extracts such as medicinal plant. The purpose of this study was to investigate the effects of ethanol extracts of Houttuynia cordata Thunb. whole plant and Calendula officinalis L. flower extracts on the growth of fibroblasts, dermal papilla cells and lipid precursors, I want to try to make a materialization. Methods and Results : The cytotoxicity of each sample extracts treated with 50%, 100%, and 500 μg to fibroblasts, cell-viability were 107.3%, 109.6%, and 128.2%, respectively. The cytotoxicity of each sample to the dermal papilla cells was not observed. And the lipid differentiation of the lipogenic precursor cells which regulates the hairegeneration by secretion of the platelet derived growth factor. The 70% ethanol extracts of H. cordata whole plant and C. officinalis flower were showed promotes the hair growth activity. The lipolysis rate was significantly increased with increasing treatment concentration Conclusion : As a result of this study, in-vitro hair growth activity of herbal medicines for hair treatment material development was not shown to be toxic to each cell. And 70% ethanol extract of H. cordata whole plant stimulated lipid precursor cells inducing differentiation. As a result, the 70% ethanol extracts of H. cordata whole plant and C. officinalis flower have potential to developing hair-related product.

Background : Black Non-Woven Fabric Mulch Culture was knowned increased crop Yield and saved weeding labor in Liriope Pilatyphylla Wang et Tang. But to the removal and planting labor is more needed, So some famers are avoidance that culting method. Methods and Results : So this study was experimented in order to selecting optimun removal time in Liriope Pilatyphylla Wang et Tang mulch culture. Removal time were conventional practices (in April next yesr), September, October and November. In early, Plant length, Root length, Leaf number and number of plants was the long and many by the sooner removal time and also, dry weight was heavier. Black non-woven fabric removal labor was saved by the sooner removal time. The main event of weeds were Cyperus serotinus Rottb and Portulaca oleracea L. In harvest time, Plant length and plants of numbers was the longest and heavier at conventional practice (in April next year) and November removed. Tuberous root number was the more in September removal, Because, the tuber was tall and long. Total1y consideration of the including weeds shooting, weeding labor and Growth and development situation, Black non-woven fabric removal optimum time was September or Conventional practices (in April next year). Conclusion : Black non-woven fabric optimum removal time was the september . In this experiment, increased yield 9, income 16 percent than conventional practices (in April next year).

Background : Natural Mortality Vinyl much culture are increased crop yield and saved weeding workforce. But research is insufficient that of Liriope Pilatyphylla Wang et Tang. Black non-woven fabric mulch culture are increased crop yield and superior to occurrence of weeds, but to the removal and planting more labor needed, so, Farmers are avoidance using that. Methods and Results : So this study designed in order to selecting the best covering material. Using in this study, covering materials were Conventional practices (non covering), Natural mortality vinyl and black non-woven fabric. Soil temperature was continued highly after planting from in mid-may to in august a regular. among them natural mortality vinyl mulch was the highest. Natural mortality vinyl mulch was the fastest that humidity of soil change and Growth and development situation by maintaining proper temperature and humidity. Non mulching was little change in temperature and humidity and the slowest in the Growth and development situation. The main event of weeds were Cyperus serotinus Rottb and Portulaca oleracea L. Weeding labor was saved from 43 to 57 percent in the natural mortality vinyl and black non-woven fabric mulch. Plant length was the shortest in the conventional practices and Root length, Leaf number and number of plants were little changed. Yield was increased from 27 to 29 percent in the natural mortality vinyl and black non-woven fabric mulch than conventional practices. Result of comprehensive economic analysis including weeding labor and yield, the natural mortality vinyl mulch culture was income increased 92 percent than conventional practices. Conclusion : Natural mortality vinyl mulch culture were weeds shooting controled and increased yield 39, income 92 percent than conventional practices (non covering).

This study aims to present direction for management of Korea Garden Show based on a survey of 105 experts in garden from a variety of backgrounds. Considerations for improving management of Korea Garden Show are as follows. First, when inviting applications for Garden Show, presenting a specific theme such as existing management method can encourage people to present more ideas from limited diversity. Second, it is the greatest time to hold the Garden Show from April to May in consideration of the diversity of plants and flowering time, and two week period is considered to be sufficient for the show according to the matters of operation and management of the show garden and requests of experts. Third, the show should be held in a designated place and after promoting sustainability and improving public awareness of the show. It should be also developed to show extended base of a garden and a variety of local characteristics, and held in provincial and municipal areas. Forth, industry-linked contents should be strengthened in the process of organizing the garden show. A symposium should be organized to introduce materials of garden industry including high-tech materials and new plant varieties and provide information about garden trends, and the show should perform its role as a platform for establishing garden culture.

Sexual dimorphism is the most conspicuous difference between the sexes. This study examines possible sexual dimorphism and the relative growth patterns of morphometric characteristics in the marine medaka, Oryzias dancena for their potential to help differentiate between males and females of this species. The von Bertalanffy growth parameters estimated by a non-linear regression method were L∞=30.2 mm, K=3.22/year, and τ0=-0.05. All 18 characteristics measured showed a difference between males and females from 70 days after hatching. Each of these characteristics were significantly different between sexes (ANCOVA, P<0.05), and the ratio of standard length between sexes showed that males were larger than females for all five morphometric measurements. Fin length measurements were taken for 21 distances of anal fin and 7 distances of dorsal fin between landmarks. There were all differences for all dorsal fin rays between the males and the females and there is significant difference in 70 days after their hatch when the sexual dimorphism is presented. The significant difference (P<0.05) in fin ray for male and female was more greatly seen as they grow. Male marine medaka showed more rapid growth than females, with longer length, dorsal fins and anal fins. Differences in these characteristics will be useful during experiments when it is necessary to differentiate between sexes of marine medaka.

The optimum concentrations of clove oil as an anesthetic for olive flounder (Paralichthys olivaceus) and the stress response of the fish to clove oil anesthesia were determined over a range of water temperatures, and investigated in a simulated transport experiment using analysis of various water and physiological parameters. While the time for induction of anesthesia decreased significantly as both the concentration of clove oil and water temperature increased, the recovery time increased significantly (P<0.05). The plasma cortisol concentration in fish at each temperature increased significantly up to 12 h following exposure (P<0.05), then decreased to 48 h (P<0.05). The DO dissolved oxygen concentrations, pH values, and the fish respiratory frequencies decreased over 6 h following exposure to clove oil in all experimental groups (P<0.05), whereas the NH4 + and CO2concentrations in all experimental groups increased up to 6 h (P<0.05). The pH values and DO concentrations increased with increasing clove oil concentration (P<0.05) in the 6 h following exposure, and the CO2 and NH4 + concentrations and the respiratory frequencies decreased with increasing clove oil concentration (P<0.05). The results of this experiment suggest that clove oil reduced the metabolic activity of olive flounder, thus reducing NH4 + excretion and O2 consumption. In conclusion, clove oil appears to be a cost-effective and efficient anesthetic that is safe for use and non-toxic to the fish and users. Its use provides the potential for improved transportation of olive flounder.

This study describes results on sexual maturation and characteristics of natural spawned eggs to develop a method for the production of stable, healthy fertilized eggs from captive-reared yellowtail kingfish, Seriola lalandi. A total of 59 yellowtail kingfish were captured off the coast of Jeju Island, after which the broodstock was cultured in indoor culture tank (100 m3) until they were 6.1–14.9 kg in body weight. As part of the rearing management for induced sex maturation, the intensity of illumination was maintained at 130 lux. The photoperiod (light/dark; L/D) was set to a 12 L/12 D from October 2013 to January 2014, and 15 L/9 D from February 2014 to June 2014. Feeds comprised mainly EP (Extruded Pellets), with squid cuttlefish added for improvement of egg quality, and was given from April to June 2014. The first spawning of yellowtail kingfish occurred in May 3, 2014, at a water temperature of 17.0°C. Spawning continued until June 12, 2014, with the water temperature set at 20.5°C. Time of spawning was 26 times at this period. The total number of eggs that spawned during the spawning period was 4,449×103. The buoyant rate of spawning eggs and fertilization rate of buoyant eggs during the spawned period were 76.1% and 100%, respectively. The diameters of the egg and oil globule were 1.388 ± 0.041 mm and 0.378 ± 0.029 mm, respectively, which was higher in early eggs than in those from late during the spawned period.

This study monitored the morphological development of embryo, larvae and juvenile yellowtail kingfish, Seriola lalandi, for their aquaculture. The fertilized eggs obtained by natural spawning were spherical shape and buoyant. Fertilized eggs were transparent and had one oil globule in the yolk, with an egg diameter of 1.35 ± 0.04 mm and an oil globule diameter of 0.32 ± 0.02 mm. The fertilized eggs hatched 67–75 h after fertilization in water at 20 ± 0.5°C. The total length (TL) of the hatched larvae was 3.62 ± 0.16 mm. During hatching, the larvae, with their mouth and anus not yet opened. The yolk was completely absorbed 3 days after hatching (DAH), while the TL of post-larvae was 4.72 ± 0.07 mm. At 40 DAH, the juveniles had grown to 30.44 ± 4.07 mm in TL, body depth increased, the body color changed to a black, yellow, and light gray-blue color, and 3–4 vertical stripes appeared. At 45 DAH, the juveniles were 38.67 ± 5.65 mm in TL and 10.10 ± 0.94 mm in body depth. The fish were green with a light orange color, with 7 faint green-brown stripes on the sides of their body. At 87 DAH, the juveniles had grown to 236.11 mm in TL, 217.68 mm in fork length, and 136.5 g in weight. The fish resembled their adult form, with a light yellow-green body color, loss of the pattern on the sides of their body, and a yellow coloration at the tip of the caudal fin.

The landmark-based morphometric and meristic analysis of the kelp grouper (Epinephelus bruneus), red spotted grouper (E. akaara) and seven-banded grouper (E. septemfasciatus) were performed to compare the differentiation of overall body shape and structure. The measurements of the morphometric dimensions were observed in 25 parts (truss dimension: 16 parts; head part dimension: 9 parts) of 38 morphometric dimensions and also meristic differences observed in 3 parts (dorsal fin, anal fin and caudal fin) of 6 meristic counts (P < 0.05). Observed morphometric characteristics primarily involved in truss and head part dimension, kelp grouper have larger values in caudal part of truss dimension, kelp grouper, red spotted grouper and seven-banded grouper have similar values in pectoral part of truss dimension, in addition to, results of head part dimension showed that red spotted grouper have smaller values in overall dimensions (P < 0.05). As meristic characteristics, kelp grouper have more number of anal fin rays than other fish, red spotted grouper have more number of dorsal soft rays than other fish, and seven spotted grouper have more number of anal soft rays, and caudal fin rays than other fish (P < 0.05). Photographed under the x-ray, kelp grouper have the most curved vertebral column and largest swim bladder than other fishes (P < 0.05). Our results of this study confirmed that 3 subfamily fishes adequately can distinguish with external body shape, and we hope that the results of our study could be used to identify in Serranidae family as taxonomical parameters.

Major loci controlling flowering time and maturity of short-day plant soybean, E1, E2, E3, E4, E5, E6, E7 and E8, have been identified in soybean. The gene corresponding to E2 locus is a homolog of Arabidopsis GIGANTEA (AtGI). We identified three GI homologs in soybean and are verifying their roles in day-length dependent flowering. Expression anlysis indicated that GmGIs are ubiquitously expressed at all developmental stages of soybean plants. Diurnal expression of GmGIs fluctuates within light/dark cycles of long-day (LD) and short-day (SD). GmGI2 and GmGI3 have identical expression patterns under both day length conditions with the highest peak at zeitgeber time 8 h (ZT8) under LD and at ZT4 under SD. GmGI1 shows the peak at ZT12 under LD and at ZT8 under SD. All of GmGIs exhibit the earlier peak and the shorter phase under SD than LD. The results indicated that day length affects expressions of GmGIs. Subcellular localization analysis showed that GmGIs are mainly targeted to nucleus, similar to the localization of AtGI. Overexpression of GmGIs in Arabidopsis transgenic plants showed no significant effect on flowering time nor rescue of gi-2 mutant phenotype. The results suggested that GmGIs have different molecular functions in flowering time regulation of short-day plant soybean compared to long-day plant Arabidopsis. To investigate the molecular mechanisms of GmGIs’ functions in soybean flowering time control, we intend to identify target gene of GmGIs and interacting proteins by using yeast two-hybrid assay.

FT is one of the major floral activator in photoperiod-dependent flowering pathway. To understand the role of FT homologs in flowering time control of short-day plant soybean, we identified ten soybean FT genes and named GmFTs. Phylogenetic analysis revealed that ten GmFT genes were further categorized into three subclades. Gene expression analysis showed that the most GmFT genes are mainly expressed in leaves. The expression of GmFT2a, GmFT2b, GmFT5a, and GmFT6 was strongly induced under the floral inductive short-day condition, but GmFT4 exhibited opposite expression pattern compared to those of GmFT2a, GmFT2b, GmFT5a, and GmFT6. To understand roles of GmFT genes in flowering, we generated Arabidopsis transgenic plant overexpressing GmFT genes. Both 35S:GmFT2a and 35S:GmFT5a transgenic plants showed extremely early flowering. In contrast, overexpression of GmFT4 delayed flowering of transgenic plants compared to wild type Arabidopsis. The results indicated that GmFT2a and GmFT5a might function as floral activators, while GmFT4 has an opposite function in soybean flowering. Moreover, domain swapping approaches between GmFT2a and GmFT4 revealed that the substitution of the segment B region alone, which is located in 4th exon, was sufficient to change the function of GmFT2a to floral repressor and GmFT4 to floral activator. The results suggested that soybean FT homologs have been functionally diversified during evolution and might play different roles in photoperiod-dependent flowering of soybean.

The roots of Platycodon grandiflorum are massively used in traditional herbal medicine as a remedy for pulmonary disease and respiratory disorders. However, in spite of its potential medicinal significance, the molecular mechanism of its roots is still unknown. In the present study, high throughput proteome approach was conducted to profile proteins from 3, 4 and 5 months aged diploid and tetraploid roots of Platycodon grandiflorum. Two dimensional gels stained with CBB, a total of 68 differential expressed proteins were identified from diploid root out of 767 protein spots using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 29 differential expressed protein spots (≥ 2-fold) were analyzed using LTQ-FTICR MS whereas a total of 24 protein spots were up regulated and 5 protein spots were down-regulated. On the contrary, in the case of tetraploid root, a total of 86 differential expressed proteins were identified from tetraploid root out of 1033 protein spots of which a total of 39 differential expressed protein spots (≥ 2-fold) were analyzed using LTQ-FTICR MS whereas a total of 21 protein spots were up regulated and a total of 18 protein spots were down-regulated. It was revealed that the identified proteins from the explants were mainly associated with the nucleotide binding, oxidoreductase activity, transferase activity. In that way, the exclusive protein profile may provide insight clues for better understanding the characteristics of proteins and metabolic activity in various explants of the economically important medicinal plant Platycodon grandiflorum.

Breeding and cultivation techniques are being treated very severely regarding ecological and physiological development in buckwheat. This study was conducted to focus on the diversity occurring in the cultivated and tartary buckwheat and provide an overview of the characteristics and genetic resources activities. Morphological results showed that the height of common buckwheat ranges from 82-90cm, common buckwheat induced by 200Gy ranges from 52-75cm, common buckwheat induced by 300Gy ranges from 43-56cm, common buckwheat induced by 400Gy ranges from 33-60cm whereas the tartary buckwheat hight ranges from 65-87cm, and while it exposed to various radiation (200Gy, 300Gy and 400Gy), the obtained height ranges from 73-92cm, 55-80cm and 60-75cm respectively. However, the stems from the both cultivar are hollow and that’s why, the plant is very prone to lodging. The leaf color of common buckwheat was green, 200Gray, 300Gy 400Gy common buckwheat light green and green, whereas the tartary buckwheat green and bottle-green, 200Gray 300Gy 400Gy tatary buckwheat bottle-green, common buckwheat (control, 200Gy, 300Gy, 400Gy) stem color is light green and pink, flower color is white, tartary buckwheat (control, 200Gy, 300Gy, 400Gy) flower color is light green. The stem color from tartary buckwheat showed (200Gy, 300Gy, 400Gy) light green and light red color. The results revealed that the two buckwheat cultivars showed diversified characteristics.