아보카도는 에콰도르에서 경제적으로 중요한 과수이다. 건강한 종묘 생산은 아보카도 식물의 생산에서 중요한 단계이며 유익한 미생물의 처리는 식물체의 영양을 향상 시키는데 사용 될 수 있다. 아보카도 묘목에 처리한 미생물의 효과를 검정한 결과, 묘목의 뿌리부분과 지상부의 양분 흡수를 증가시켰다. T. harzianum은 뿌리에서 N과 Mg의 흡수를 유의적으로 증가시켰으며, 지상부에서는 N, Ca, Mg, Mn 및 Cu의 흡수를 증가시켰다. 반면에, G. iranicum var tenuihypharum은 뿌리에서 Ca와 Fe의 흡수를 유의적으로 증가시켰으며, 지상부에서는 N흡수를 증가시켰다. 또한, mycorrhiza의 접종으로 아보카도 묘목의 뿌리 부분과 지상부에서 P 양을 증가시키는 경향이 긍정적으로 나타났다. 두 미생물 모두 S의 흡수에 영향을 미치지 않았는데, 이는 식물 전체에서 균일한 비율을 나타냈다. 또한 인산질 비료(일인산 칼륨)의 시용은 미량 영양소의 흡수에 긍정적인 영향을 미쳤다.

In this study, we examined the protective immunity of a combination of seven Brucella abortus recombinant proteins; superoxide dismutase (rSodC), riboflavin synthase subunit beta (rRibH), 50S ribosomal protein (50s rL7/L12), nucleoside diphosphate kinase (rNdk), malate dehydrogenase (rMDH), arginase (rRocF), and elongation factor (rTsf) cloned in a pMal vector system and expressed in DH5α. Mice groups were immunized thrice with a combined subunit vaccine (CSV-7) at 0, 2, and 5 weeks and subsequently challenged with B. abortus at 5 × 104 CFU at 6 weeks. At four weeks post-infection, the mice were sacrificed and the bacterial burden in their spleens was quantified. Results revealed bacterial log reductions of 0.63 and 0.34 in comparison to PBS and maltose-binding protein (MBP), respectively. Cytokine profiling revealed a marked increase in IFN-γ (interferon-gamma), MCP-1 (macrophage chemoattractant protein-1) and IL-6 (interleukin 6) cytokines at 5-weeks post-immunization. On the other hand, only TNF was heightened at 7-weeks post-immunization. In general, this cytokine profile is consistently reflective of a Th1 immune response, which is beneficial for host immunoresistance.

원심 해수냉각 펌프를 분석하기 위하여 다른 운전 유량에 대한 캐비테이션 거동을 조사하였다. 3D 2상 해석은 ANSYS-CFX 상 용코드로 수행되었다. 해석에는 k-ε 난류와 Rayleigh-Plesset cavitation 모델이 사용되었다. 수치 예측에 기초하여 세 가지 토출 유량값에 대 하여 헤드 드롭 특성곡선이 작성되었다. 더 높은 유량에서 임펠러는 버블 캐비테이션에 보다 취약하다. 0.7Q, Q 및 1.3Q(Q: 설계 유량)에서 작동하는 펌프의 3 % 헤드 드롭 위치는 각각 NPSHa 1.21 m, 1.83 m 및 3.45 m에 해당한다. 증기 기포의 볼륨이 예측되고 캐비테이션의 위치는 임펠러 내에서 발생하는 캐비티를 시각화하여 예상하였다. 또한, 압력계수와 날개 부하 분포가 구체적으로 제시되어 캐비테이션이 펌프 운전에 미치는 해로운 영향을 나타냈다. 또한, 압력계수 분포와 날개부하 차트가 구체적으로 제시되어, 펌프 운전에 캐비테이션이 미치는 해로운 영향을 나타냈다.

Intensive Monitoring Survey of Nearby Galaxies (IMSNG) is a high cadence observation program monitoring nearby galaxies with high probabilities of hosting supernovae (SNe). IMSNG aims to constrain the SN explosion mechanism by inferring sizes of SN progenitor systems through the detection of the shock-heated emission that lasts less than a few days after the SN explosion. To catch the signal, IMSNG utilizes a network of 0.5-m to 1-m class telescopes around the world and monitors the images of 60 nearby galaxies at distances D < 50 Mpc to a cadence as short as a few hours. The target galaxies are bright in near-ultraviolet (NUV) with MNUV < - 18.4 AB mag and have high probabilities of hosting SNe (0.06 SN yr-1 per galaxy). With this strategy, we expect to detect the early light curves of 3.4 SNe per year to a depth of R  19:5 mag, enabling us to detect the shock-heated emission from a progenitor star with a radius as small as 0.1 R. The accumulated data will be also useful for studying faint features around the target galaxies and other science projects. So far, 18 SNe have occurred in our target fields (16 in IMSNG galaxies) over 5 years, confirming our SN rate estimate of 0.06 SN yr-1 per galaxy.

The health benefits associated with consumption of fresh produce have been clearly demonstrated and encouraged by international nutrition and health authorities. However, since fresh produce is usually minimally processed, increased consumption of fresh fruits and vegetables has also led to a simultaneous escalation of foodborne illness cases. According to the report by the World Health Organization (WHO), 1 in 10 people suffer from foodborne diseases and 420,000 die every year globally. In comparison to other processed foods, fresh produce can be easily contaminated by various routes at different points in the supply chain from farm to fork. This review is focused on the identification and characterization of possible sources of foodborne illnesses from chemical, biological, and physical hazards and the applicable methodologies to detect potential contaminants. Agro-chemicals (pesticides, fungicides and herbicides), natural toxins (mycotoxins and plant toxins), and heavy metals (mercury and cadmium) are the main sources of chemical hazards, which can be detected by several methods including chromatography and nano-techniques based on nanostructured materials such as noble metal nanoparticles (NMPs), quantum dots (QDs) and magnetic nanoparticles or nanotube. However, the diversity of chemical structures complicates the establishment of one standard method to differentiate the variety of chemical compounds. In addition, fresh fruits and vegetables contain high nutrient contents and moisture, which promote the growth of unwanted microorganisms including bacterial pathogens (Salmonella, E. coli O157: H7, Shigella, Listeria monocytogenes, and Bacillus cereus) and non-bacterial pathogens (norovirus and parasites). In order to detect specific pathogens in fresh produce, methods based on molecular biology such as PCR and immunology are commonly used. Finally, physical hazards including contamination by glass, metal, and gravel in food can cause serious injuries to customers. In order to decrease physical hazards, vision systems such as X-ray inspection have been adopted to detect physical contaminants in food, while exceptional handling skills by food production employees are required to prevent additional contamination.

프리지아 ‘Sweet Lemon’은 2007년 농촌진흥청 국립원예특작과학원에서 흰색 겹꽃 ‘Teresa’품종과 노랑색 반겹꽃 ‘Yvonne’ 품종을 교배하여 획득한 종자로부터 2007년 연노랑색 향기가 강한 겹꽃 프리지아 계통을 선발하여 품종으로 개발되었다. 2008년부터 2014년까지 개화 생육특성검정 및 육성계통평가회의 기호도 평가를 통해 선발되어 2015년 ‘Sweet Lemon’ 으로 명명되었다. ‘Sweet Lemon’은 연노랑색(RHS color chart Y2B) 겹꽃 프리지아 품종으로 화폭은 6.8cm이며 분지수는 6.7개로 다수확성 품종이다. 초장은 99cm로 초세가 강하며 대조품종 ‘Yvonne’ 91.7cm에 비해 34.7cm 더 크다. ‘Sweet Lemon’의 소화수 및 소화장은 각각 10.7개, 7.7cm로 ‘Yvonne’ 9.7개, 8.3이며 개화소요일수는 126일로 대비품종 보다 약 20일 정도 빠르다. 이 품종의 절화수명은 약 8.7일이며 자구번식력은 5.6배로 대조품종에 비해 우수하다. GC/MS를 이용한 향기분석 결과 총 58개의 방향성 화합물이 검출되었으며 주요성분은 linalool, alpha-Terpineol, alpha-Selinene, limonene이었다.

This study evaluated the protective effects of a combination of eight B. abortus recombinant proteins that were cloned and expressed into a pMal vector system and DH5α: nucleoside diphosphate kinase (rNdk), 50S ribosomal protein (rL7/L12), malate dehydrogenase (rMDH), DNA starvation/stationary phase protection protein (rDps), elongation factor (rTsf), arginase (rRocF), superoxide dismutase (rSodC), and riboflavin synthase subunit beta (rRibH). The proteins were induced, purified, and administered intraperitoneally into BALB/c mice. The mice were immunized three times at weeks 0, 2, and 5 and then infected intraperitoneally (IP) with 5×104 CFU of virulent B. abortus 544 one week after the last immunization. The spleens were collected and the bacterial burden was evaluated at four weeks post-infection. The results showed that this combination produced a significant reduction of the bacterial burden in the spleen with a log reduction of 1.01 compared to the PBS group. Cytokine analysis revealed induction of the cell-mediated immune response in that TNF (tumor necrosis factor) and proinflammatory cytokines IL-6 (Interleukin 6) and MCP-1 (macrophage chemoattractant protein-1) were elevated significantly. In summary, vaccination with a combination of eight different proteins induced a significant protective effect indicative of a cell mediated immune response.

In our previous studies, the cardiac xenotransplantation from an alpha-1,3-galactosyltransferase knockout pig (GT-MCP-MCP) to cynomolgus monkeys showed a mean survival of 38 days. The objective of this study is to genetically upgrade the GT-MCP-MCP pig, to further enhance membrane cofactor protein (MCP) expression and to express an endothelial specific thrombomodulin (TBM). MCP is a complement regulatory protein and TBM is a coagulation inhibitor. As the dicistronic cassette for wild-type-based MCP and TBM concurrent expressions does not show any increase of MCP, we optimized the MCP codon usage (mMCP) and substituted mMCP for MCP. When the mMCP-TBM cassette was transfected to HeLa cells, we were able to find an increased expression of MCP and endothelial cell-specific TBM expression. The cassette was then transfected into ear-skin fibroblasts isolated from one-month-old #23-4 of a GT-MCP-MCP pig, and the cell populations expressing MCP were obtained by MACS cell sorting. We performed a single cell culture of the selected cells, and obtained clones over expressing 90% MCP. The cells of a clone were used as a donor for nuclear transfer and generated GT-MCP/-MCP/mMCP/TBM pig. The transgenic pig was confirmed to be carrying the cells expressing MCP and functioning as an inhibitor against the cytotoxic effect of normal monkey serum, comparable with donor cells. Thus, we believe that the GT-MCP/-MCP/mMCP/TBM transgenic pig would be potential for the prolongation of xenograft survival in the recipients.