우리나라는 무기질비료 사용량이 많은 나라 중 하나이다. 그러나 1980년 이후 지속가능농업에 대한 필요성에 의해 1997년 친환경 농업육성법이 제정되었고 지속가능한 농업을 위한 연구로 논, 밭, 시설 및 과수에 대한 비료사용실태조사가 실시되고 있다. 이에 본 연구는 과수 중 대표되는 7작물(배, 포도, 사과, 복숭아, 대추, 단감, 키위)을 대상으로 각 도에서 805농가를 선정하여 실시하였으며, 조사내용은 사용하는 비료의 종류, 사용량 등에 대해 농가를 방문하여 면접 청취조사하였다. 국내 과수작물의 성분별 평균 비료사용량(N-P2O5-K2O)은 30.0-23.8-24.3 kg 10a-1이었으 며 비료 종류별로 구분하였을 때 퇴비 > 유기질비료 > 무기질비료 순으로 사용하고 있었다. 단위면적당 가장 많은 비료를 사용하고 있는 과수는 대추로 질소, 인산 및 칼리를 평균 53.3-42.0-49.5 kg 10a-1을 사용하고 있는 것으로 확인되었다. 앞으로 지속가능 농업과 농업환경의 보전을 위해서는 농가에서 사용하는 비료의 장기간 모니터링 뿐만 아니라 농업인에게 무기질비료, 유기질비료 및 퇴비의 적정사용방법에 대한 교육 및 홍보를 지속적으로 실시해야 될 것으로 판단된다.

Metallothioneins (MTs) are low molecular weight, cysteine-rich, and heavy metal binding proteins, which could be induced with heavy metals such as Cd, Hg, Zn and Cu in liver, kidney, and in cultured cells. By using ion exchange chromatography on DE-52, MT was purified from the serum of carp induced with cadmium in order to produce antibody against MT. Polyclonal antibody produced against purified carp MT reacted well with MT in the serum of carp induced with cadmium, whereas control serum did not. This may indicate that the polyclonal antibody against the carp MT could be used for the preparation of biosensors to detect MT in fishes like carp.

겉뿌림초지 개량효과를 높이기 위해 질소시비수준과 보조초종 라이그라스 조합의 필요성 여부를 구명키 위해 4처리 질소시비수준(0, 100, 200, 300kg/ha)과 3처리 혼파조합(A; orchardgrass+ladino clover, B; orchardgrass+ladino clover+perennial ryegrass, C; orchardgrass+ladino clover+perennial grass+Italian ryegrass)으로 본 시험이 수

This study was conducted to investigate the responses of soil properties and microbial communities to different agricultural management and soil types, including organic management in Andisols (Org-A), organic management in Non-andisols (Org-NA), conventional management in Andisols (Con-A) and conventional management in Non-andisols (Con-NA) by using a pyrosequencing approach of 16S rRNA gene amplicon in Radish farms of volcanic ash soil in Jeju island. The results showed that agricultural management systems had a little influence on the soil chemical properties but had significant influence on microbial communities. In addition, soil types had significant influences on both the soil chemical properties and microbial communities. Organic farming increased the microbial density of bacteria and biomass C compared to conventional farming, regardless of soil types. Additionally, Org-NA had the highest dehydrogenase activity among treatments, whereas no difference was found between Org-A, Con-A and Con-NA and had the highest species richness (Chao 1) and diversity (Phyrogenetic diversity). Particularly, Chao 1 and Phyrogenetic diversity were increased in organic plots by 12% and 20%, compared with conventional plots, respectively. Also, regardless of agricultural management and soil types, Proteobacteria was the most abundant bacterial phylum, accounting for 21.9-25.9% of the bacterial 16S rRNAs. The relative abundance of putative copiotroph such as Firmicutes was highest in Org-NA plot by 21.0%, as follows Con-NA (13.1%), Con-A (6.7%) and Org-A (5.1%.), respectively and those of putative oligotrophs such as Acidobacteria and Planctomycetes were higher in Con-A than those in the other plots. Furthermore, LEfSe indicated that organic system enhanced the abundance of Fumicutes, while conventional system increased the abundance of Acidobacteria, especially in Non-andisols. Correlation analysis showed that total organic carbon (TOC) and nutrient levels (e.g. available P and exchangeable K) were significantly correlated to the structure of the microbial community and microbial activity. Overall, our results showed that the continuous organic farming systems without chemical materials, as well as the soil types made by long-term environmental factors might influence on soil properties and increase microbial abundances and diversity.

The JK-1 isolate which was the best producer of indole-3-acetic acid and carotenoid among the 388 strains isolated from 28 wetlands in Jeju, was identified to be Rhodopseudomonas palustirs belongs to a typical group of non sulfur purple bacteria based on 16S sRNA sequencing. This study investigated the effect of different cultural conditions of pH, temperature, agitation, light and aeration on growth, IAA and carotenoid production of photosynthetic bacterium JK-1 for optimization of IAA and carotenoid production. It was found that growth, IAA, carotenoid, and bacteriochlorophyll production with light (3,000~3,500 Lux) and agitation (100 rpm) showed better results than those with dark/static or dark/ agitation (100 rpm) in anaerobic conditions. The optimal pH, temperature and agitation speed for cell growth were 7, 30℃, 150 rpm, for IAA production were 9, 30℃, 150rpm and for carotenoid production were 6, 25℃, 50 rpm, cultured for 72 h under anaerobic light, respectively. The growth and IAA production were high in aerobic culture compared with anaerocic culture, whereas carotenoid and bacteriochlorophyll content were decreased extremely in aerobic condition (0.5~1 vvm). Subsequently, the optimal culture conditions for JK-1 were selected with pH 7, 30℃ and 100 rpm under anaerobic light and the effect on plant growth was tested by pot assay. Inoculation of JK-1 with 3% (v/v) level caused increase in shoot and root dry weigh that varied from 20%~58% to 40%~28% in young radish in camparison to uninoculated treatment at 50 days of growth. The study suggests that the JK-1 isolate may serve as efficient biofertilizer inoculants to promote plant growth.

본 연구는 제주지역 화산회토 감귤원에서 두과피복작물인 헤어리베치(Vicia villosa Roth) 의 이용 가능성을 진단하고 효율적인 양분관리 체계를 정립하기 위하여 수행하였다. 시험 은 감귤원 재배지 고도별 5개소(해발고도:신촌 37 m, 호근 116 m, 와산 123 m, 상예 161 m, 그리고 용강 232 m)에서 수행하였다. 헤어리베치 품종은 국내에서 개발된 ‘청풍보라’로 하였으며, 파종량은 ha당 30, 60 및 90 kg을 파종하였다. 시험결과 헤어리베치 입모율은 53.0~57.1%로 파종량과 고도별 포장에 관계없이 유사하였다. 파종량에 따른 잡초 발생비율 은 5개소 평균 11.8, 3.8 및 5.1%로 처리간 유의성이 없었으나 30 kg 파종구에서 다소 높은 경향을 보였으며 60 kg 및 90 kg 파종구는 각각 96.2% 및 94.9% 정도의 잡초 경감효과를 나타냈다. 헤어리베치 파종량에 따른 ha당 건물 수량은 각각 7.37, 8.43 및 8.47톤으로 30 kg 대비 60 및 90 kg 파종 시 각각 14% 및 15% 높았으나 파종량별 유의성은 없었다. 질소 수 량은 ha당 최소 175 kg (와산 30 kg ․ ha-1 파종구)에서 최대 467 kg (용강 30 kg ․ ha-1 파종구) 값을 얻었으며 파종량에 따른 5개소 평균 질소 수량은 각각 254, 316 및 315 kg으로 60과 90 kg 파종구간 차이는 없었으며, 30 kg 파종구에 비하여 24% 높았다. 감귤원에서 기상과 토양 화학성 등 재배환경 요인들이 헤어리베치 수량에 미치는 영향을 구명하기 위하여 요 인들 간 상관관계를 분석한 결과 일평균기온(R=0.2746) 및 적산온도(R=0.2714) 등 기상요인 은 헤어리베치 건물 수량에 다소 영향을 줄 수는 있으나 그 영향이 크지 않은 것으로 판단 되었다. 반면에 토양 EC (R=0.4520**), 치환성칼륨(R=0.4078**) 및 유효인산(R=0.2737) 함량 등이 높을수록 건물 수량이 증가하는 비교적 강한 양의 상관관계를 보여 기상요인 보다는 토양 화학성이 헤어리베치 수량에 미치는 영향이 큰 것으로 판단되었다. 따라서 헤어리베 치 재배시 질소 수량은 포장별 여러 가지 재배적 환경요인들의 영향을 받을 수 있으므로 포장별 일정면적의 생초 수량(X)을 측정한 후 이에 따른 질소 수량(Y)을 산출하여 활용하 는 것이 바람직할 것으로 보이며 그 산출식은 ｢Y=4.4097X+33.594 (R=0.9547)｣와 같다.

This study was conducted to determine the effects of organic fertilization rates on the nutrient accumulation and recovery in radish (Raphanus sativus L.) as well as growth and yield of radish in Jeju island. An understanding the relationships between organic fertilization rate, crop nutrient recovery and crop yield can assist in making organic fertilizer recommendation which balances crop value and environmental risk in organic cultivation. Nitrogen (T-N), phosphate (P2O5) and potassium (K2O) were applied at 0, 115-35-40, 230-70-80 (standard application rate), 460-140-160, 230-200-100 (recommended application rate) and 158-53-35 kg/ha (customary application rate), respectively as the broadcast application of mixed organic fertilizer (N 4.5% - P2O5 1.5% - K2O 1%) in combination with langbeinite (K2O 22%), 100% at sowing period. The organic fertilizer was made of organic materials like oil cakes. Total yield of radish, as fresh weight of roots, increased with increasing organic fertilizer doses to a maximum at rate of standard or soil-testing application. Nitrogen, phosphate and potassium accumulations of radish increased curvilinearly with increasing organic fertilization rate to a maximum at rate of N 460 - P2O5 140 - K2O 160 kg/ha. However, nitrogen, phosphate and potassium use efficiency of applied organic fertilizer decreased curvilinearly or linearly with increasing organic fertilization rate. Application of organic fertilizer in combination with langbeinite (as a potassium source) had significant effect on the yield of radish. Organic fertilization on a basis of standard or soil-testingapplication rate is recommended for maximun radish yield in organic cultivation.