This study was conducted to investigate for mineral contents, total polyphenol compounds,betaine, choline and DPPH free radical scavenging activity of halophyte. The mineral concentrations of Salicornia herbacea (top part) were Na 100,006 mg/kg, K 1,385 mg/kg, Mg 6,263 mg/kg, Ca 2,750 mg/kg, Fe 90.4 mg/kg, Mn 98.9 mg/kg, Zn 33.3 mg/kg, Cu 3.4 mg/kg respectively. And Suaeda Japonica (top part) were Na 85,332 mg/kg, K 710 mg/kg, Mg 7,005 mg/kg, Ca 4,344 mg/kg, Fe 1,434.9 mg/kg, Mn 119.1 mg/kg, Zn 19.2 mg/kg, Cu 2.7 mg/kg respectively. The betaine contents of Salicornia herbacea (top part) were 15.09 mg/g and Suaeda Japonica (top part)were 14.64 mg/g. The choline contents estimated by the DBAP-choline derivatives of Salicornia herbacea (top part)were 20.9 mg/100 g, Salicornia herbacea (root) were 23.4 mg/100 g, Suaeda Japonica (top part) were 23.1 mg/100g and Suaeda Japonica (root) were 23.8 mg/100 g. Total polyphenol compounds of Salicornia herbacea (top part) were high 36.0 mg/g in growth phase. The DPPH radical scavenging activities of methanol extract Salicornia herbacea (top part) were high 90.1% in growth phase. The frozen dried powder of Salicornia herbacea (top part) 1 g was equal to Quercetin 30.26 mg, Rutin 42.65 mg, TBHQ 20.32 mg, BHA 25.86 mg, BHT 40.75 mg, Ascorbic acid 22.86 mg in DPPH radical scavenging activities.
Erwinia herbicola(EH) 채소 연부병 방제를 위하여 충북 청원군, 진천군 및 충남 연기군 일원의 채소 경작지 토양으로부터 형광성을 나타내는 Pseudomonas속 1, 196균주를 선발하고, 연부병균 Erwinia herbicola에 대한 생육억제력과 생물학적 검정으로 발병억제력이 강한 2균주를 선발하여 Pseudomonas fluorescens S4(PS4)와 Pseudomonas fluorescens S65 (PS65)로 동정하였다. 배춧잎을 이용하여 분리길항균의 연부병균에 대한 생물학적 억제력을 확인한 결과 상당한 발병억제력을 확인하였다. 이들 두 균주가 가장 높은 길항력을 나타내는 조건은 배양 초기배지의 pH를 7.0으로하여 25℃에서 3일간 배양했을 때였다. 화학농약 및 항생물질에 대해 2균주 모두 benomyl, propamocab hydrochloride, fosethyl-Al-folpet, vancomycin, penicillin 및 lincomycin에 내성을 나타냈고, PS4균주는 erythromycin에 대해서도 내성을 나타냈다.
Piperine, component of pure ground black pepper, has strong stimulative and trot. Analytical method for piperine was developed by high performance liquid chromatography. Analytical conditions are as follows; mobile phase is 70% methanol, detector LTV 343 nm (0.05 AuFa), column is Novapak 5 C_(10) (15 cm × 4.6 mm), flow rate is 1.0 ml/min, chart speed is 0.25 cm/ min and intjection volume is 20 Etl. Analytical results are as follows that relative standard deviation is 1.15%, calibration curve is y=170473.1×-7848.5 (R²=0.999) that shows good linearity. Standard solution of piperine is stable up to 10 hr and content of piperine in pure ground black pepper is 4.97±0.86%. Retention time of piperine in HPLC method is about 7 min. Therefore, the developed HPLC method including simple pretreatment of sample will be contribute to quality management.