This study examines the characteristics of berries from secondary bearing shoots of ‘Scintilla’ southern highbush blueberry, grown hydroponically in the Jinju, Gimhae, and Uiryeong regions of Gyeongnam Province. Typically, ‘Scintilla’ forms flower buds at the tips of regular bearing shoots during the previous season, yielding berries in the current season. However, under heated cultivation, we observed a proliferation of secondary bearing shoots that produce berries in the same growing season. Flowering and harvesting on secondary bearing shoots were delayed by 52 and 36 days, respectively, compared to regular bearing shoots. However, these shoots exhibited a 54% increase in diameter and a 10% increase in length. We found no significant difference in berry size and soluble solid content between the two types of shoots. Notably, berries from the secondary bearing shoots had higher potassium and lower calcium and magnesium concentrations. We conclude that berries from secondary bearing shoots could be marketable, provided the bushes are healthy. These findings provide valuable insights for optimizing cultural practices to improve the yield and quality of blueberries under specific environmental conditions.

For appropriate nutrient management and enhanced plant growth, soil sensors which reflect soil nutrient levels are required. Because there is no available sensor for nutrient monitoring, electrical conductivity (EC) sensor can be used to evaluate soil nutrient levels. Soil nutrient management using EC sensors would be possible by understanding the relationship between sensor EC values and soil temperature, moisture, and nutrient content. However, the relationship between soil sensor EC values and plant available nutrients was not investigated. Therefore, the objectives of the study were to evaluate effect of different amount of urea on soil EC monitored by sensors during pepper and broccoli cultivation and to predict the plant available nutrient contents in soil. During the cultivation period, soil was collected periodically for analyzing pH and EC, and the available nutrient contents. The sensor EC value increased as the moisture content increased, and low fertilizer treated soil showed the lowest EC value. Principal component analysis was performed to determine the relationship between sensor EC and available nutrients in soil. Sensor EC showed a strong positive correlation with nitrate nitrogen and available Ca. In addition, sum of available nutrients such as Ca, Mg, K, P, S and N was positively related to the sensor EC values. Therefore, EC sensors in open field can be used to predict plant available nutrient levels for proper management of the soil.

아미노글리코사이드계 항생제(Aminoglycosides, AGs) 는 그람음성균과 양성균에 광범위하게 작용하는 동물용 의약품으로, 최근 배양육에 사용된다고 알려져 있어, 안 전성 관리를 위한 분석법 마련이 반드시 필요하다. AGs 는 고극성 화합물로 성분 간의 분리를 위해 이온쌍 시 약(ion-pairing reagent, IPR)을 사용하고 있으나 IPR을 이동상에 첨가하는 기존 분석방법의 경우 용매가 흐르 는 동안 질량분석기로 주입되는 IPR로 인해 기기적인 문 제가 발생할 가능성이 높아, IPR를 바이알에 직접 첨가 하는 분석방법을 검토하였다. 본 연구에서 10종 AGs 성 분에 대한 분석방법을 확인하고 유효성을 검증하였다. 검출한계와 정량한계는 각각 0.0001-0.0038 mg/kg 와 0.004-0.011 mg/kg의 범위로 나타났으며, 0.01-0.5 mg/ kg 범위 내의 직선성(R2)은 0.99 이상이었다. AGs의 시 료 회수율을 확인하고자 소고기와 세포배양배지(cell culture medium) 매질에서 회수율과 상대표준편차로 나 타낸 정밀도를 확인한 결과 각각 70.7-120.6% 및 0.2 to 24.7%로 나타났다. 기존의 이동상에 IP 첨가 방법과 비 교하였을 때 유사한 수준으로 양호하였다. 검증된 AGs 분석법은 국내 유통되는 닭고기, 소고기, 돼지고기 15품 목과 배양육 배지 첨가제 6품목에 적용해보았다. 그 결 과 국내 유통되는 육류 15품목 모두 AGs 성분이 검출 되지 않았으나, 세포배양배지에서 streptomycin은 695.85- 1152.71 mg/kg, dehydrostreptomyci은 6.35-11.11 mg/kg 로 검출되었다. 따라서 IRR을 바이알에 직접 첨가하는 LC-MS/MS 방식은 육류, 세포배양배지, 배지첨가제 중 AGs 분석 및 안전성 평가를 위한 기초자료로 활용될 것 으로 기대된다.

Oral squamous cell carcinoma (OSCC) is the most common type of oral cancer, and it has been steadily increasing in worldwide. Pituitary tumor transforming gene 1 (PTTG1) has been known as oncogene in a verity of cancers. Nevertheless, the expression and role of PTTG1 in OSCC progression remains largely unexplored. In this study, clinical datasets were analyzed to assess the genetic impact of PTTG1 on OSCC progression and to identify its functional roles in OSCC cell lines. We analyzed the expression of PTTG1 in head and neck squamous cell carcinoma (HNSC) and OSCC using databases form the Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO). To investigate the effect of PTTG1 on proliferation and migration abilities in OSCC cell lines, following the knockdown of PTTG1 in HSC-2 and SCC-9 cell lines, we analyzed the proliferation and metastatic abilities of OSCC cells using EdU and Boyden chamber assays. Our database analysis revealed that PTTG1 was significantly overexpressed in tumor tissues compared to normal tissues. Moreover, its expression correlated with clinical parameters of OSCC. In vitro experiments demonstrated that depletion of PTTG1 suppressed the ability of cell proliferation and migration in both HSC-2 and SCC-9 cell lines. In conclusion, our study suggests that PTTG1 may act as an oncogene in OSCC. These findings provide new insights into the mechanisms and clinical implications of PTTG1 expression in OSCC patients.

이 연구는 코르크보드를 보강하여 건축부재 및 놀이기구의 안전부재 등으로 폭넓게 활용할 것을 목적으로 코르크보드의 중층에 금속, 유리섬유, 탄소섬유를 삽입하여 보강한 3종의 코르크복합보드를 제조하였고, 코르크복합보드의 수분흡수에 따른 치수안정성 및 접착층 박리성능을 조사하였다. 코르크복합보드의 흡수율은 0.37% - 0.45%의 범위에 있었고, 코르크보드에 비해 0.61배 - 0.74배의 낮은 값을 나타내었다. 코르크복합보드의 두께팽창률은 0.92% - 1.58%의 범위에 있었고, 코르크보드 보다 1.4 - 2.4배의 높은 값을 나타내었다. 그러나 이 값들은 일반 목질보드보다 현저히 낮았고, KS규격의 12%이하를 하회하는 것이 확인되었다. 코르크복합보드의 준내수 및 내수침지박리시험후의 접착층박리율은 0%로 전혀 접착층의 박리가 일어나지 않아 우수한 내수성을 나타내었고, 흡수율과 흡수두께팽창률은 상온침지에 비해 다소 증가하였으나, 목질보드에 관한 KS규격을 하회하는 우수한 치수안정성을 나타내는 것이 확인되었다.

PURPOSES : The objectives of this study are to evaluate the condition of concrete bridge decks using the multi-channel ground penetrating radar (GPR) testing and compare the value of its dielectric constant value with actual concrete condition. METHODS : The reflection coefficient method was used to measure the dielectric properties of concrete bridge decks. Air-coupled step-frequency GPR testing was used to measure the time taken for reflection from the interfaces between the layers. Specimens of the asphalt mixture and concrete bridge-deck were collected by field coring. GPR testing was conducted on two bridges with different concrete bridge deck conditions on national highways. After the GPR tests, the actual conditions of the concrete bridge deck were investigated using specimen coring. RESULTS : GPR testing indicated that the dielectric constants of concrete bridge decks in good condition ranged from 8 to 10, whereas those corresponding to poor condition ranged from 4 to 6. The results of GPR testing can determine the actual condition and degree of distress of concrete bridge decks determined from the specimen coring data. Therefore, GPR testing is appropriate for nondestructively evaluating the condition of a concrete bridge deck. CONCLUSIONS : The analysis results of the dielectric constants of the concrete bridge deck obtained from multichannel GPR testing were consistent with the actual bridge deck conditions. In the near future, an additional verification process for this approach under different bridge conditions will be required to improve its precision and ensure reliability.

PURPOSES : This study aims to analyze and summarize test results related to permeable blocks used for sidewalks and roadways as an alternative to conventional urban road pavement technology, specifically focusing on low-impact development (LID) techniques. Furthermore, it aims to provide reference data on the feasibility of current policy implementation and future policy directions through a longterm analysis of the performance and durability of permeable blocks in sidewalk and roadway construction. METHODS : The research methodology involves (1) conducting a survey on the status and actual conditions of permeability sustainability tests based on the results of permeability persistence tests conducted in Seoul over nine years, from 2013 to 2021; (2) analyzing the differences between the permeability block rating system of Seoul City and that employed by the Ministry of Environment; (3) analyzing the permeability of graded pavement sections in permeable blocks for sidewalks after three years of public use and deriving a regression analysis formula to estimate the maintenance period; (4) analyzing the permeability of pavement sections in permeable blocks for roadways after five years of public use and deriving a regression analysis formula to estimate the maintenance period. RESULTS : This study revealed significant improvements in the performance and quality control of permeable blocks since the implementation of the permeability sustainability test in Seoul in 2013. An analysis of the differences in the permeability coefficient and distribution based on the rating systems of Seoul City and the Ministry of Environment showed that rating system of Seoul City has approximately twice the permeability coefficient quality standard compared to that of the Ministry of Environment. Regression analysis indicated that the firstgrade permeable block was predicted to maintain its permeability for approximately 5.1 years, whereas the third-grade permeable block was predicted to maintain its permeability for approximately 3.1 years. In roadway-permeable block pavements, the first-grade sections maintained excellent quality conditions even after five years, and regression analysis predicted a permeability maintenance period ranging from eight to 17 years. CONCLUSIONS : The correlation analysis of the permeability coefficients based on the common usage period of permeable blocks for sidewalks and roadways presented in this study can provide more accurate estimations of commonality. However, further research under various environmental conditions is required to supplement these findings. Considering the lack of studies on permeable block commonality analysis in Korea, this study highlights the significance of conducting long-term experimental follow-up research to establish commonality prediction formulas for different usage scenarios.

국제적인 선박 배출 가스에 대한 환경 규제가 점차 강화됨에 따라 전기추진 및 하이브리드 추진선박에 대한 관심이 증대되고 있으며, 이러한 선박에 적용하기 위한 다양한 솔루션이 개발 및 적용되고 있는데 특히 전력계통의 안정화, 시스템의 효율성을 높이기 위 한 방안으로 직류배전시스템이 적용되고 있다. 또한, 선박용 직류배전시스템에 대한 안전 및 성능에 대한 검증 및 시험의 중요성이 요구 되고 있다. 본 연구는 직류배전 테스트베드 구축 및 직류배전(가변속 발전)시스템에 대한 성능을 검증하고 연료소모량 분석한 결과로서 선박용 직류배전에 적용되고 있는 가변속 발전시스템을 적용하였으며, 발전기에서 출력되는 전력을 정류기를 통해 직류전력으로 변환하 여 시스템에 연계하고 이러한 장치들을 감시 및 제어하기 위한 시스템을 개발하였다. 이러한 직류배전 시스템을 적용한 시험을 통해 최 대 전압은 751.5V이고 최소전압은 731.4V가 계측되어 전압변동률은 2.7%로 3% 이내에서 안정적으로 전압이 공급되는 것을 확인하였고 부 하변동에 따라 가변속 발전시스템을 적용하였을 경우 연료소모량이 기존 정속발전시스템과 비교하여 구간에 따라 최대 20%이상 연료절 감이 되는 것을 시험을 통해 확인하였다.

Benthic attached diatoms (BADs), a major primary producer in lotic stream and river ecosystems are micro-sized organisms and require a highly magnified microscopic technique in the observation work. Thus, it is often not easy to ensure accuracy and precision in both qualitative and quantitative analyses. This study proposed a new technique applicable to improve quality control of aquatic ecosystem monitoring and assessment using BADs. In order to meet the purpose of quality control, we developed a permanent mounting slide technique which can be used for both qualitative and quantitative analyses simultaneously. We designed specimens with the combination of grid on both cover and slide glasses and compared their efficiency. As a result of observation and counting of BADs, the slide glass designed with the color-lined grid showed the highest efficiency compared to other test conditions. We expect that the method developed in this study could be effectively used to analyze BADs and contributed to improve the quality control in aquatic ecosystem health monitoring and assessment.