This study was carried out to investigate the bat (Chiroptera) fauna in Jeju Island. Bat distribution has been monitored for last eleven years (since July 2006 to April 2016). We visited and survey the bat distribution in fifteen natural lava tubes, three sea caves, nineteen artificial cave encampments constructed during 20th century, five natural forest regions nearby Hallasan (eco-corridors) and one sea-shore area. Bat specimens were also confirmed at two local museums in Jeju Island. From the present study, we found 9 species of 6 genera belong to 3 families, which was quitely different from the records had previously described in Jeju Island since 1928. Five species (Rhinolophus ferrumequium, Myotis bombinus, Myotis rufoniger, Myotis macrodactylus and Miniopterus schreibersi) were commonly found to live in lava tubes and cave enforcements. But only Rhinolophus ferrumequium was found in a sea cave and five species including Murina leucogaster and Pipistrellus abramus were found in eco-corridors. Myotis aurascens were only observed in the cave enforcements. We have also confirmed the existence of specimens, including Tadarida insignis, stored at the local museums. From the results of this study, we actually could not found the remaining seven species (Hypsugo savii, Miniopterus fuscus, Myotis mystacinus, Myotis ikonnikovi, Myotis branditii, Myotis petax and Pipistrellus endoi). From the investigation of bat species, Myotis rufoniger which is known as one of endangered species were detected in four different locations including two lava tubes, one forest region, and one cave enforcement. This is the first time of finding for Myotis rufoniger in the artificial architecture. In addition, one of rare finding species Myotis bombinus in Korean Peninsula, which were found in several lava tubes and cave enforcements, in some cases they were observed in the large population over one-hundred individuals. The Tube-nosed bat (Murina leucogaster) was discovered for the first time through this study in 2009, which was found in the mountainous forest regions nearby Hallasan in summer season. Interestingly, Myotis aurascens were also discovered firstly in spring season of 2015. Myotis aurascens were discovered three locations of cave enforcements but the localities were restricted only in the western region of Jeju Island. Using the bat specimens fo Myotis aurascens collected from three different locations, we tried to examine the morphometric and phylogenetic relationship among the records in the East Asia. The bat population of Jeju Island had smaller levels of HBL and Hfcu, but greater levels of TL, EL, FAL, and Tra than those of the Korean Peninsula population. Jeju bats had wide range in the lengths of FAL and Hfcu comparing to those of European bats. From the bimonthly monitoring to each finding site, we have actually failed to observe those again, estimating that they use those CEs as the daily-roosting place in activating seasons. The sequences of CYTB and COI genes showed identical sequences among Jeju Myotis aurascens bats tested, indicating that they are maternally related. The results from molecular phylogeny showed that the sequences of these bats located on the same branch with those for Myotis aurascens in the phylogenetic trees. Besides, the nucleotide sequences of the Jeju Myotis aurascens bats showed the closest relation with those of Korean Peninsula. Consequently, these findings indicate that the bats of Myotis aurascens, verified the natural distribution in Jeju Island, have originated from a single maternal origin and differences in morphological and genetic backgrounds form those of Korean Peninsula and the other countries, and had probably immigrated via Korean Peninsula from the northwest. The results of this study showed that it should be carried out to reconstruct the bat fauna and to reveal the natural habitation status of each species in this island in the future. In addition, these findings will contribute as basic information for understanding the migration history and biogeographic relationship of bats in East Asia, and for planning the conservation and management programs of the wildlife in Jeju Island.

This study was conducted to screen the characteristics of external and cranial characters of Crocidura shantungensis collected from Korean Peninsula, Ulleung Island and Jeju Island. There were significant differences in head-body length (HBL), tail length (TL), ear length (EL), and hind foot length (HFL) in males and HBL, TL, and HFL in females among three populations (p<0.05). The HBL and TL in the Jeju Island population were larger (8.0 mm and 4.0 mm in lengths, respectively) than those of the Korean Peninsula population. Based on skull analysis, a total of thirteen traits showed significant differences among the three populations (p<0.05). The condylo-insicive length (CIL), maximum width of brain case (MWB), mandibular length (ML), and mandibular height (MH) in the Jeju Island population were significantly larger than those of Korean Peninsula population (p<0.05). Principle component analysis (PCA) showed that two principle components (PC) identified from the PCA affected on the body size and width of the skull, respectively. Discriminant analysis revealed that these populations could be discriminated through skull traits. These findings concluded that the Jeju Island population was greater in the size than the Korean Peninsula, suggesting that the Jeju Island shrews have successfully adapted to the island environments and they had morphologically differentiated during glacial period after natural immigration into that Island. Thus, this study supports the ‘Island Rule’, showing that the population is well adapted to the island environments. This may provide important information for biogeographical and ecological studies on insular animals.

This study was carried out to investigate the bat (Chiroptera) fauna in Jeju Island. Bat distribution was monitored in lava tubes, sea caves, cave encampments and eco-corridors from July, 2006 to June, 2015. Bat specimens were also confirmed at the museums in Jeju Island. From the present study, we found 8 species of 6 genera belong to 3 families, which was different from the records had previously described in Jeju Island since 1928. Five species (Rhinolophus ferrumequium, Myotis bombinus, M. formosus, M. macrodactylus and Miniopterus schreibersi) were commonly found to live in lava tubes and cave enforcements. But only R. ferrumequium was found in a sea cave and five species including Murina leucogaster and Pipistrellus abramus were found in eco-corridors. We have also found seven species including Tadarida insignis in the specimens stored in local museums. From the results of this study, we actually could not found the remaining seven species (Hypsugo savii, Miniopterus fuscus, Myotis mystacinus, Myotis ikonnikovi, Myotis branditii, Myotis petax and Pipistrellus endoi). Interestingly, Myotis formosus which is known as one of endangered species and Myotis bombinus which is very rare in Korean Peninsula were currently found in Jeju Island. In addition, Tube-nosed bat (Murina leucogaster) that was discovered for the first time through this study. A variety of habitats for Chiroptera were identified, but the information is still not sufficient to understand. These results will be useful to provide a fundamental data in preserving the diversity of bats and in ecological study in Jeju Island.

동절기에 제주도 지역에서 도래하는 떼까마귀의 유전적 특성과 집단 간 유연관계를 구명하기 위해, 미토콘드리아 COI 유전자 서열의 다형성에 기반한 모계 계통 구조와 계통 유연관계를 분석하였다. 떼까마귀 DNA는 우도와 제주도 내에서 발견된 깃털과 사체 시료에서 분리하였다. 결정된 COI 서열들(n=41)은 떼까마귀(Corvus frugilegus)에서 기존 에 보고된 서열들과 97.0% 이상 일치하였다. 제주도 떼까마귀 COI 서열들은 3가지 haplotype(J01-J03)으로 구분되었으 나 지역-특이적인 양상을 보이지 않아, 이들이 하나의 모계 기원에서 유래한 집단임을 알 수 있었다. 떼까마귀 전체 COI 서열에서 8개의 COI haplotype들이 발견되었다. 이 중 3가지 haplotype들은 러시아 동부, 몽골, 한국 등 동북아시 아의 COI 서열들을 포함하였고, 나머지 5가지는 중앙아시아, 중동아시아, 러시아 서부, 유럽국가의 떼까마귀에서 발견 되었다. 계통수 상에서 떼까마귀의 COI 서열들은 측소적 종분화 단계인 2아종, C. f. frugilegus와 C. f. pastinator인 2개의 모계 계통으로 뚜렷하게 구분되었다. DNA barcoding 분석을 통한 연구결과는 모계 계통의 구조, 계통 유연관계 및 분자생태를 이해하는 데 중요한 정보를 제공할 것이다.

In this study, we report for the first time a hybridization between Caretta caretta and Chelonia mydas in South Korea. On 7th May 2012, a sea turtle (Jeju sea turtle 01, JST01) was found in the east coast of Jeju Island, South Korea. The morphological characteristics of JST01 were compared with those of C. caretta and C. mydas that were frequently observed in Korean Seas. Interestingly, JST01 showed similar morphological characters such as body color, appearance of carapace, shape of upper beak, serration on marginal scute, and two claws with those of C. caretta. On the other hand, JST01 showed also similar patterns in several characteristics including the numbers of marginal, inframarginal and costal scute, smooth carapace without keel, no contact between costal scute and nuchal scute with those of C. mydas. From these results, we suggest that JST01 is one of hybrid animals bred between C. caretta and C. mydas, because JST01 has speciesspecific characters from both species. We anticipate that our study would be useful and have significant impact onto study inter-specific hybridization in the groups of sea turtle.

한국산 집쥐속(Rattus)에 속하는 동물들을 2005년 4월부터 2012년 10월까지 제주도의 각처에서 채집하여 형태학적, 유전학적 종 동정과 형태적 특성, 2차 성적이형, 서식지 유형 등을 조사하였다. 연구결과, 제주도에서 포획된 Rattus 속 야생쥐들은 Rattus norvegicus와 R. tanezumi로 확인되었고, R. rattus는 확인되지 않았다. R. norvegicus는 제주도 내 각처에서 특히, 주택 밀집지역의 인가 내-외부에서 채집되었고, 일부는 자연환경인 해안이나 경작지, 산림에서 관찰되기도 하였다. R. tanezumi는 동물 사육 농장의 내외부에서만 확인되었다. 형태적 특성으로 R. norvegicus는 꼬리길이(TL)와 귀의 길이(EL)가 R. tanezumi에 비해 짧고, 이들 형질의 머리-몸통 길이의 비율인 T_HR, E_HR 등도 유의적인 차이를 보였다(p<0.05). 인구밀집 지역인 도심과 농촌 지역의 R. norvegicus와 개체군 사이에서는 도심지역 개체군의 체중이 더 무거운 것으로 확인되었다(p<0.05). R. norvegicus에서는 2차 성적이형 현상이 나타나지 않았으나, R. tanezumi는 암컷이 수컷에 비해 더 무거운 것으로 나타났다(p<0.05). 현재까지 보고된 연구결과와는 다소 다른 종의 분포와 서식환경을 보여주고 있는 본 연구 결과는 향후 추가연구를 통해 제주도와 한반도 포유류상의 재검토와 함께 서식지 분포 양상과 생태적 특성 등을 명확하게 구명해야함을 시사하고 있다.

한국산 집쥐속(Rattus)에 속하는 동물들을 2005년 4월부터 2012년 10월까지 제주도의 각처에서 채집하여 형태학적, 유전학적 종 동정과 형태적 특성, 2차 성적이형, 서식지 유형 등을 조사하였다. 연구결과, 제주도에서 포획된 Rattus 속 야생쥐들은 Rattus norvegicus와 R. tanezumi로 확인되었고, R. rattus는 확인되지 않았다. R. norvegicus는 제주도내 각처에서 특히, 주택 밀집지역의 인가 내-외부에서 채집되었고, 일부는 자연환경인 해안이나 경작지, 산림에서 관찰되기도 하였다. R. tanezumi는 동물 사육 농장의 내외부에서만 확인되었다. 형태적 특성으로 R. norvegicus는 꼬리길이(TL)와 귀의 길이(EL)가 R. tanezumi에 비해 짧고, 이들 형질의 머리-몸통 길이의 비율인 T_HR, E_HR 등도 유의적인 차이를 보였다(p<0.05). 인구밀집 지역인 도심과 농촌 지역의 R. norvegicus와 개체군 사이에서는 도심지역 개체군의 체중이 더 무거운 것으로 확인되었다(p<0.05). R. norvegicus에서는 2차 성적이형 현상이 나타나지 않았으나, R. tanezumi는 암컷이 수컷에 비해 더 무거운 것으로 나타났다(p<0.05). 현재까지 보고된 연구결과와는 다소 다른 종의 분포와 서식환경을 보여주고 있는 본 연구 결과는 향후 추가연구를 통해 제주도와 한반도 포유류상의 재검토와 함께 서식지 분포 양상과 생태적 특성 등을 명확하게 구명해야함을 시사하고 있다.

This study was carried out to examine a molecular marker system for parentage test in Jeju Black cattle (JBC). Based on the preliminarily studies, we finally selected for construction of a novel genetic marker system for molecular traceability, identity test, breed certification, and parentage test in JBC and its related industrial populations. The genetic marker system had eight MS markers, five indel markers, and two single nucleotide polymorphisms (SNPs; g.G299T and g.del310G) within MC1R gene which is critical to verify the breed specific genotypes for coat color of JBC differing from those of exotic black cattle breeds such as Holstein and Angus. The results showed lower level of a combined non-exclusion probability for second parent (NE-P2) of 4.1202×10-4 than those previously recommended by International Society of Animal Genetics (ISAG) of 5.000×10-4 for parentage, and a combined non-exclusion probability for sib identity (NE-SI) of 2.679×10-5. Parentage analysis has been successfully identified the JBC offspring in the indigenous population and cattle farms used the certified AI semens for production using the JBC-derived offspring for commercial beef. This combined molecular marker system will be helpful to supply genetic information for parentage test and traceability and to develop the molecular breeding system for improvement of animal productivity in JBC population.

This study was carried out to investigate synthetic extender for semen cryopreservation of Jeju Native Black Bull. The semen was collected using an artificial vagina and transported to the laboratory. The semen was diluted 1:1 by Tris-Egg yolk extender and contrifuged in 1,500 rpm for 15 minutes. The supernatant was removed. The pellect was diluted to final sperm concentration of 2×108/ml by doubling in every 30 minutes at 4℃ cold chamber. The semen was equilibrated for 4 hours at cold chamber and packed to 0.5 ml straw. The semen straws were located above 5 cm for 10 minutes. The height and duration affect the freezing speed by temperature. The frozen straw was plunged to LN2. The presented straws were examined the viability and motility after thawed at 37℃ water bath. Frozen-thawed sperm were evaluated sperm viability, membrane integrity and acrosome integrity. Post-thawed sperm viability has been significantly higher (p<0.05) in fresh sperm (93.27±1.62%) than frozen-thawed sperm (73.34±3.27%). However, there were no significant differences between fresh and frozen-thawed dead cell rate (7.35±2.63 vs, 13.71±2.85). In sperm motility, between Triladyl and AndroMed Extender, there was no significant different (72.86±2.83 vs, 81.47±2.48), similarly, the dead cell rates was similar (18.41±3.42% and 17.26±4.25). The results of our study suggest that AndroMed to the freezing extender showed more positive effect on the frozen-thawed spermatozoa in Jeju Native Black bull semen.

본 연구는 경상북도 청도군 운문산 자연휴식년제 지역일대의 포유류상을 밝히기 위해 2007년 12월부터 2009년 9월까지 수행되었다. 조사결과 6목 11과 24종의 포유류의 서식을 확인하였다. 우점종으로는 고라니 Hydropotes inermis, 다람쥐 Tamias sibiricus, 두더지 Mogera wogura, 흰넓적다리붉은쥐 Apodemus peninsulae 등이었다. 특히, 천연기념물(NM)과 환경부지정 멸종위기종(ES)인 하늘다람쥐(Pteromys volans; NM #328, ES II급), 수달(Lutra lutra; NM #330, ES I급), 삵 (Prionailurus bengalensis; ES II급), 담비 (Martes flavigula; ES II급) 등 법적보호종 4종이 발견되었다. 다양한 포유류 종과 법적보호종의 서식에 대한 지리적 분포를 확인한 이 결과는 조사지역이 포유류의 서식에 필요한 자연생태계의 매우 양호한 조건을 갖추고 있는 지역임을 반영한다. 반면, 관리동물 종으로 지정된 들고양이가 다수 관찰되었다. 운문산 자연 생태계의 건전한 유지와 생태계 위해 동물의 관리 방안을 마련하기 위해서는 지속적이고 장기적인 연구조사가 필요하다.

제주도에서는 절멸된 것으로 간주되었던 멧돼지들이 최근 한라산 인근지역에서 발견되었다. 본 연구는 분자유전학적 실험기법을 바탕으로 한라산 멧돼지들이 가축돼지들과 이종교배된 것들인지를 조사하였다. 또한 동일 종내에서의 유전적 유연관계와 분자 성판별을 시험하였다. 가축돼지 품종들(Landrace, Large White, Berkshire, Hampshire, Duroc)과의 교배여부는 핵 DNA와 미토콘드리아 DNA에서 4 종류의 분자 표지인자(MC1R, KIT, 조절영역, ND2)를 적용하여 시험하였다. 야생멧돼지 집단의 모든 개체들이 동일한 mtDNA 조절영역 서열을 나타내었고, 그 서열들은 중국 동북부 재래돼지들과 동일하였으나 기존에 보고된 한반도 멧돼지의 서열들과는 다른 것으로 확인되었다. 이상의 연구결과는 한라산 멧돼지집단이 중국 재래돼지 품종들과 근연이면서, 기존에 연구되지 않았던 유전적 계통에서 유래한 것으로 사료된다. 분자 성판별 결과 수컷에 비해 암컷이 2 배 이상으로 확인되어, 한라산 야생멧돼지 집단이 팽창하고 있으며, 조절하지 않으면 집단 규모는 극적으로 증가할 것이다.

The aim of this study was to evaluate the effect of α-tocopherol on blastocysts development and subsequent cryosurvival of the vitrification. The α-tocopherol(0, 100, 200, 400 μM) was added in to culture medium for the bovine embryos. The blasocysts from the α-tocopherol and untreated control groups were then frozen-thawed, and their cryosurvival was assessed by in vitro culture for 48 h. There were no differences in the overall cleavage rate(56.14±4.66, 58.18±4.70, 62.97±6.86 and 51.17±7.28) among four treatment groups. However, in blastocyst development and total cell number were significantly higher in α-tocopherol 200 μM(38.60±7.12; 106.33±3.50) to culture medium than other treatment groups(29.30±5.24, 31.60±7.12 and 26.37±4.18; 101.36±5.12, 97.27±2.87, and 91.23±7.52 respectively). Before and after vitrification, the total cell number and blastocyst development of embryo were significantly higher in July to August than September to October. In conclusion, addition of α-tocopherol 200 μM to in vitro bovine embryo culture medium was beneficial for improving embryo quality by decreasing the embryo damage blsstocysts cell number and improving the tolerance of the embryos to cryopreservation.

포유동물 수정란의 동결보존기술은 최근 기후 변화에 따른 생물종 다양성을 보존하기 위해서 중요하게 여겨지는 연구 분야이다. 따라서 멸실 위험에 처한 동물의 개량과 증식, 보존과 복원 및 생명공학의 분야에 이르기까지 응용 기술은 다양하게 이용되어진다. 본 연구에서는 한우 수정란의 동결 후 생존성 향상을 위해서 동결 방법에 따른 체내 외수정한의 내동성을 조사하였다. 완만동결에 따른 체내 외수정란의 동결 융해 후 수정란의 재확장률은 89.6%와 81.5% 그리

본 연구는 8주령 Sprague-Dawley계통의 암컷 랫드에서 제독유황을 반복경구투여 독성평가를 하기위해 시행하였다. 랫드에 처치한 물질은 사료섭취량의 0.2%, 1%, 5% 제독유황과 사료섭취량의 1% 유황으로 13주 경구투여를 하였다. 제독유황의 안전성을 확인하기 위해 다음과 같은 관찰 및 검사를 하였다. 검사항목으로는 체중과 사료 섭취를 측정하였고 임상증상, 안과학적 검사, 혈액학적 검사, 혈청 생화학적 검사를 관찰하였다. 또한 랫드에 간과 신장에서 의 병리조직학적 변화를 관찰하였다. 그 결과 체중, 사료섭 취량, 혈액학적 및 병리조직검사 소견에서 대조군과 제독 유황 그룹 간의 유의적인 차이가 보이지 않았다. 혈청생화 학적 검사소견에서는 0.2% 및 1% 투여군에서는 대조군과 비교시 용량의존적으로 유의적인 차이를 보이는 항목은 보이지 않았으나, 고용량군에서는 glucose 감소에 의한 저칼 륨혈증, 인슐린 과잉분비의 독성을 의심할 수 있었으며, 또 한 ALT와 ALP의 증가는 간 독성이나 간암, 황달의 유발이 의심되었다. 한편, 병리조직학적인 검사에서는 시험 전체군에서 대조군과 비교시 유의할만한 병리조직이상이 관 찰되지 않았다. 따라서 본 연구에서는 사료섭취량 1%의 제독유황까지 투여는 랫드에서 독성을 나타내지 않는 것으로 사료된다.