The sustainability of fishing villages is threatened by manpower shortages due to population aging and poor settlement conditions. In the reality of poor fishing villages, the importance of women's labor is more emphasized than that of the past because female workers are key workers in fishing labor, processing and sales of marine products. However, policy support for female fishermen is not sufficient. The reason for this is that policy makers did not properly recognize the labor value of female fishermen. In fact, fishing villages have emphasized the importance of female workforce as a slogan, but there has been no attempt to estimate the labor value. There was not even a review of the methodology for estimating the value of labor that was attempted in similar fields. As a result, the policy importance of female fishermen was underestimated for there was no attempt to evaluate their value even though women had been continuously participating in the fishery from the past. Female fishermen’s labor is under the dual labor structure of housework and fishing labor. Therefore, in this study, housework and fishing labor were estimated separately and the total labor value was calculated. The basic data necessary for estimating the labor value of female fishermen were obtained through a survey. The method of estimating the labor value of female fishermen was used in combination with the present income method and the total replacement cost method. As a result of the study, the total labor value of female fishermen was about 4.4 trillion won, which is about half of the total fishery production of 9 trillion won in Korea.

In this study, the acute toxicity of Dendropanax morbiferus H.Lév leaf hot-water extracts (DMWE) was examined in male and female ICR mice. Mice were orally administered the DMWE at dose levels of 0, 250, 500, 1,000 and 2,000 mg/kg body weight (BW) for single-dose toxicity test. There were no significant differences in change of BW between control and all DMWE treated-groups. In hematological and blood biochemical analysis, none of the parameters were affected by the DMWE. Similarly, there were no significant effects on markers for liver and kidney functions in all DMWE treated-groups. Since there were no adverse effects of the DMWE in single oral toxicity tests, even at the highest doses, it was concluded that the lethal dose 50 (LD50) of DMWE is estimated at > 2,000 mg/kg BW.

The Deep Borehole Disposal (DBD) method has various advantages, such as minimizing the use of site area and corrosion of the disposal container and improving long-term structural safety. However, it is necessary to review the problems that may occur in various technologies related to the emplacement and retrieval of the disposal container and the sealing of the borehole. Therefore, the purpose of this study is to evaluate the structural integrity of an emplacement and retrieval device (hereinafter, the disposal container connecting device) of a DBD container. The disposal connecting device was evaluated according to ANSI 14.6 and NUREG-0612 standards. The allowable stress should be less than the yield strength under the load condition of 3g. The length of the disposal container connecting device was about 2,900 mm, the diameter was 406 mm, and the weight was about 1.2 tons. In addition, 10 disposal containers weighing up to 2.2 tons were handled. The disposal container connecting device was made of stainless steel, and the maximum operating temperature was about 300°C. For structural evaluation, ABAQUS finite element analysis program was used. The analysis model was modeled only 1/2 part considering symmetry condition. The analysis model was modeled using 410,431 nodes and 344,119 solid elements. Three times load was applied to the weight of the disposal container. Axisymmetric conditions were applied to the symmetrical surface of the disposal container, and vertical restraints were applied to the upper lifting lugs. A surface-to-surface contact condition was applied to the part where the contact occurred. As a result of the analysis, the greatest stress was generated at the part supported by the clamp at the disposal container connector at 168.9 MPa. In the lugs and pins connecting the guide and the connecting device, a stress of 530.1 MPa was generated by shearing. In the bolts of the disposal container connecting device, a stress of 498MPa was generated and the safety margin was 1.73. A stress of 486.1 MPa was generated in the disposal container connecting device, and the safety margin was the smallest 1.16. As a result of the analysis, all components of the disposal container connecting device showed a safety margin of 1.16 or more at the maximum operating temperature and satisfied the allowable stress.

The present study is designed to investigate the antibacterial effect of the hot-water and various ethanol extracts from the leaves of Dendropanax morbifera L. (DML) against Porphyromonas gingivalis (P. gingivalsis). Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 30, 50 and 70% ethanol extract of DML against P. gingivalis decreased in a concentration-dependent manner. However, MIC and MBC of hot-water and 30% ethanol extract against P. gingivalis were the same as 3.13 and 6.25 mg/mL, respectively. In the bacterial growth inhibition test, the growth of P. gingivalis in the group treated with MBC and 2×MBC of DML hot-water extract was statistically significantly decreased from 6 h after incubation compared to the control group (p<0.001). From the results portrayed above, aqueous extract from DML at the concentration of 6.25 mg/mL can be usefully used to suppress infection of P. gingivalis, a major causative agent of periodontal disease.

The purpose of this study is to understand the production efficiency of individual fishing communities and provide directions for improvement. The subject of the study is aquaculture type Ochon-Gye in Goheung-gun. The analysis method used bootstrap-DEA to overcome the statistical reliability problem of the traditional DEA analysis technique. In addition, data mining-GIS was applied to identify the spatial productivity of fishing communities. The values of technology efficiency, pure technology efficiency, and scale efficiency were estimated for 32 aquaculture-type fishing villages. Then, using the benchmarking reference set and weights, the projection was presented through adjustment of the input factor excess, and furthermore, the confidence interval of the efficiency values considering statistical significance was estimated using bootstrap.

The ultimate purpose of this study is to revitalize the fishing community as the basis for the revival of the fisheries industry. The types of Eochon-Gye acceptance were analyzed as a basic study to achieve this purpose. According to the results of the study, it turned out that the head and the member of the Eochon-Gye had the same three Eochon-Gye acceptance types; that is, democratic, decision-making, and separatist types. However, the Eochon-Gye acceptance types by two groups differed in detail, even though they were named by the same names. Therefore, in order to devise an effective revitalization plan of the fishing community, it is necessary to reflect the detailed characteristics of each Eochon-Gye acceptance type owned by the two groups.

가시오가피의 첨가는 영지버섯 균사체를 이용한 분비 단백질 라카아제 및 셀룰라아제의 생산량을 효율적으로 증가시켰다. 영지버섯 균사체의 생장률 측정에서 가시오가피의 첨가는 영지버섯 균사의 생장률이 톱밥에 비해 절반 수준에 밖에 못미치는 결과를 나타내었다. 반면에 영지버섯 균사의 분비 단백질인 라카아제와 셀룰라아제의 양을 증가시키는데 톱밥에 비해 큰 효과가 있음을 확인할 수 있었다. 가시오가피가 첨가된 배양액에서의 라카아제 활성은 톱밥이 첨가된 배양액에서보다 영지 1호, 영지 2호 및 녹각 영지에서 0.61~2.37배 증가하였고, 이 중에서도 영지 2호는 0.947 U/min으로 라카아제 활성이 가장 높게 평가되었다. 셀룰라아제 활성 평가에서는 가시오가피가 첨가된 배양액에서의 셀룰라아제 활성은 톱밥이 첨가된 배양액에서보다 영지 1호, 영지 2호 및 녹각 영지에서 1.77~2.81배 증가하였고, 이 중에서도 영지 1호 및 영지 2호에 비해 녹각 영지에서 0.172 U/min으로 셀룰라아제 활성이 가장 높게 평가되었다. 따라서 가시오가피의 첨가가 영지버섯 균사의 분비 단백질 생산을 증가시키는데 유용한 소재로 사료된다.

최근 소득이 증가하고 건강에 관심이 증가함에 따라 단순한 미용 화장품보다 여러 가지 기능성을 가진 화장품에 대한 관심이 높아지고 있다. 이에 따라 각종 천연물에 대하여 항산화, 항염 및 미백 등의 활성을 검증하는 다양한 연구가 진행되고 있으며, 본 연구에서도 천연물의 활성과 더불어 다양한 약리작용이 있는 영지버섯 균사체와의 발효에 의한 시너지 효과를 규명하고자 하였다. 인진쑥 영지균사발효 추출물의 DPPH radical 소거 활성은 영지버섯 균사추출물과 유사하게 농도 의존적으로 증가하는 것이 확인되었으나, 저농도의 인진쑥 추출물 보다는 다소 감소하는 것이 확인되었다. 인진쑥 영지균사발효 추출물의 RAW 264.7 세포의 NO 생성 억제활성을 통한 항염활성 평가에 앞서 세포 생존율을 평가한 결과, 흥미롭게도 인진쑥 추출물은 150 μg/mL의 농도부터 RAW 264.7 세포에 대한 생존율을 유의적으로 크게 감소시켰으나, 영지 균사 발효 시 세포 생존율이 크게 증가하는 것이 확인되었다. 영지 균사 발효 시 인진쑥의 독성이 크게 감소하는 것이 확인되었다. 또한 인진쑥 추출물 25 μg/mL의 처리는 동일 농도의 인진쑥 영지버섯 균사발효 추출보다 오히려 유의적인 수준에서 RAW 264.7 세포의 NO생성을 억제하는 것이 확인되었다. 그러나 인진쑥 영지버섯 균사발효는 인진쑥 추출물 자체의 RAW 264.7 세포에 대한 독성을 저감하는 효과가 있어 이로 인해 더욱 높은 농도의 영지버섯 균사발효 추출물 처리를 가능할 것으로 사료된다. 영지 균사 발효에 의한 미백 기능성 소재 발굴이라는 본 연구의 취지에 부합하는 결과가 인진쑥 영지균사발효 추출물 처리구에서 확인되었으며, 200 μg/mL 농도에서는 양성 대조군인 arbutin 보다 약 3.66배의 활성을 보였다. 이러한 결과는 향후 인진쑥 영지균사발효 추출물이 tyrosinase활성을 억제하는 기능성 소재로서의 활용가능성이 크다는 것을 의미하는 것이다.

민물가마우지 Phalacrocorax carbo는 사다새목 Pelecaniformes 가마우지과 Phalacrocoracidae 의 조류이며, 무리를 이루어 번식하는 것으로 알려져 있다. 2003년도와 2008년도에 한강하구와 서해 도서지역에서 민물가마우지의 집단번식이 보고된 이 후, 김포대교 부근과 인천 서만도, 경기도 양평, 춘천 의암호, 수원 서호 등 국내 내륙 지역으로 번식지가 확산되고 있다. 최근 번식지의 확대와 개체수의 증가로 영소목의 고사(산림 훼손), 저수지와 양어장의 어업피해 등 직･간접적인 피해가 나타나고 있다. 그러나 실질적인 피해와 규모에 대해서는 아직 보고된바 없으며, 민물가마우지의 번식생태 및 이동 확산에 대한 기초자료 또한 매우 미흡한 실정이다. 본 연구는 2013년부터 2018년 6월까지 국내의 민물가마우지 번식지에 대한 자료 및 현지 조사를 통하여 국내 번식지 분포현황을 확인하였으며, 번식지 규모와 서식지 이용특성에 대해 분석하였다. 국내 민물가마우지 번식지는 강화 옹진군 서만도, 충남 보령 나무섬, 전북 군산 닭섬, 금란도, 경기도 가평 팔당호, 춘천 의암호, 원주 매지저수지, 수원 서호, 충주 충주호, 괴산 감물면, 제천 월림저수지, 영동 미전수지, 안동 반변천 등으로 확인되었으며, 주로 규모가 큰 강, 저수지, 바닷가 등지에 위치한 고립된 섬에 번식지가 형성되어 있었다. 민물가마우지의 번식지 규모의 변화 및 번식지 식생 등을 알아보기 위해서는 번식지 내부로의 접근성이 유리한 지역을 선정하여 조사를 실시하였으며, 번식지의 식생은 아까시나무 및 신갈나무 등의 활엽수 군락지이며 번식지 인근은 소나무, 일본잎갈나무, 자작나무, 참나무 등이 군락을 이루고 있었다. 또한 넓은 농경지와 경작지, 저수지 등의 수계 등이 넓게 분포하고 있었다. 번식지는 2013년 부터 현재까지 지속적으로 번식지 규모의 변화에 대해서 확인하였으며, 첫해에는 야산의 450.40m2 의 규모의 번식지 였으며, 2017년에는 약 2,000m2 규모가 번식지로 이용되었다. 또한 민물가마우지의 둥지이용은 새로운 둥지를 매년 짓는 비율이 29.7% 로 가장 높게 나타났으며, 작년에 사용한 둥지를 재사용하는 비율이 24.5% 로 두 번째로 높게 나타났다. 또한, 이소한 민물가마우지 새끼새의 이동 및 서식지의 이용특성을 알아보기 위해서 국내의 번식지 중 접근성과 채집에 유리한 지역인 충북 괴산군 감물면에 위치한 번식지에서 이소한 새끼새를 채집하여 위치추적기(WT-300, 한국 환경생태연구소)를 부착하고 추적 및 오랜 기간 머문 장소에 대해서 서식지 분석을 하였다. 위치추적기는 5월 말과 6월 초에 부착하였으며, 매일 2시간 단위로 위치를 기록하고, 오후 12시와 16시에 2차례 수신되게 설정하였다. 위치 추적기는 총 5개체에 부착하였으며, 5월 31일에 1개체, 6월 4일 3개체, 6월 5일 1개체 부착하였다. 개체확인을 위해 nwlc1801∼nwlc1805의 코드를 부여하였다. 부착 후, 5개체 모두 약 한달 동안 번식지와 번식지 인근의 저수지를 오가며 활동하는 것이 확인되었다. 이 후 원인미상 등의 이유로 1개체는 수신이 끊겼으며, 1개체만 현재까지 지속적으로 수신되고 있다. 추적기가 부착된 nwlc1805번 개체는 번식지를 떠나, 약 135km를 남하하여 현재 대구 달성군에 위치한 달성습지에서 머물러 있는 것이 확인되었다. 이러한 연구는 국내의 민물가마우지 번식지 분포와 민물가마우지 번식지 확산에 대한 기초자료에 활용될 수 있을 것으로 판단된다.

The primary purpose of this paper is to introduce the cases of fishery co-management, particularly in Korea. In recent, the fishery co-management between the government and fishermen has been brought an important shift of paradigm in a part of Korean fishery management policy. In case of Korea fisheries area, three fisheries cooperatives(which refer to purse sein fishery, trawl fishery, stow net fishery) have a minority of co-management programs. Generally, the main method of fishery co-management programs is ‘Fishery Closures’ among the programs, and others are restriction of fishing gear and fishermen educations. In spite of the efforts and time consumes of Korean government and fisheries cooperatives, much to our regret that it is hard to confirm visible results in an increased amount of fisheries resources so far because of the time constraint, namely fishery co-management programs have been conducted just since two to three years before. However, what’s certain is that fishery co-management programs make it possible to positively effect on both of area in fishermen and fisheries resources. Accordingly, it is necessary that Korean government and fishermen should extend the fishery co-management to other fishery areas as soon as possible we can in order to efficiently reduce administrative costs and effectively reserve fisheries resources.

The primary purpose of this paper is to analyze the membership of fishing community. In order to become a member of fishing community, some conditions are needed both the member of local fisheries cooperatives and resident of fishing village. Generally, many fishing communities have additional conditions such as to be a member of fishing community that is a minimum term of residence, an entrance fee, fishing abilities. These additional conditions play a critical role in making a relationship, building trust and enhancing fairness. However, the setting of excessive additional conditions make it possible to obstruct growth of fishing community.

This paper is concerned with a test method that can be used to investigate the parameters of the Johnson-Cook constitutive model. These parameters are essential for accurately analyzing material behavior under impact loading conditions in numerical simulation. Ti-6Al-4V alloy (HCP crytal structure) was used as a specimen for the experiments. In the 10−3-103/ s strain rate range, three types of experimental methods (convention, compression and tension) were employed to compare the differences using MTS-810, SHPB and SHTB. Finite element analysis results when applying these parameters were displayed along with the experiment results.

One of the issues currently facing nuclear power plants is how to store spent nuclear waste materials which are contaminated with radionuclides such as 134Cs, 135Cs, and 137Cs. Bioremediation processes may offer a potent method of cleaning up radioactive cesium. However, there have only been limited reports on Cs+ tolerant bacteria. In this study, we report the isolation and identification of Cs+ tolerant bacteria in environmental soil and sediment. The resistant Cs+ isolates were screened from enrichment cultures in R2A medium supplemented with 100 mM CsCl for 72 h, followed by microbial community analysis based on sequencing analysis from 16S rRNA gene clone libraries (NCBI’s BlastN). The dominant Bacillus anthracis Roh-1 and B. cereus Roh-2 were successfully isolated from the cesium enrichment culture. Importantly, B. cereus Roh- 2 is resistant to 30% more Cs+ than is B. anthracis Roh-1 when treated with 50 mM CsCl. Growth experiments clearly demonstrated that the isolate had a higher tolerance to Cs+. In addition, we investigated the adsorption of 0.2 mg L-1 Cs+ using B. anthracis Roh-1. The maximum Cs+ biosorption capacity of B. anthracis Roh-1 was 2.01 mg g-1 at pH 10. Thus, we show that Cs+ tolerant bacterial isolates could be used for bioremediation of contaminated environments.

In our previous study, active-single compound (ASC), inhibitor of melanogenesis in B16F10 melanoma cell, was identified from Ganoderma lucidum. Thus, the aim of this study is to analyze the change of the ASC contents in G. lucidum when lacquer tree (LT) was added in cultivation of G. lucidum. In HPLC analysis, significantly increased ASC peak was observed in G. lucidum extract supplemented with 15% (v/v) of LT. In addition, melanogenesis inhibitory effect of G. lucidum extract supplemented with 15% of LT significantly increased when compared to G. lucidum extract without LT supplementation. Furthermore, LT supplementation increased mycelial growth of G. lucidum in both solid and liquid cultivation. These results suggest that is useful as natural ingredient for increasing bioactivity including skin-whitening effect and mycelial growth of G. lucidum.

지진시 사회 인프라시설물의 피해는 시설물 자체의 피해보다 사회 전반에 걸친 2차 피해를 야기한다. 그 중, 지하 공동구 구조물은 통신, 가스, 전기 등 사회의 라이프라인에 해당하여 지진에 대한 취약성을 정확히 평가하여야 할 필요가 있다. 따 라서, 본 연구에서는 지하 공동구의 지진 발생 지반가속도에 따른 파괴가능성을 평가하였다. 평가를 위한 입력지반운동은 해 외 실측 지진데이터와 한반도에서 발생가능한 인공지진파를 차용하였으며, 지진해석 방법은 응답변위법과 시간이력해석법 을 사용하였다. 파괴여부를 판별하는 한계상태는 휨모멘트와 전단 파괴를 바탕으로 하였다. 취약도 함수 도출을 위한 방법은 최우도법이 사용되었으며, 그 분포함수는 대수정규분포로 가정하였다. 이는 지진시 지하 공동구 시설물의 피해 평가는 물론 지하 공동구 시설물의 내진설계를 위한 기초자료로 활용될 수 있다.

Ganoderma lucidum, a species of the class Basidiomycetes, attracts international attention due to the wide variety of its biological activities and great potential as cosmetic ingredient, such as skin care cosmetics including ‘skin-whitening’. However, there is little information available regarding the tyrosinase inhibitory activity. Therefore, the objectives of this study were to investigate the chemical composition and tyrosinase inhibitory activity of the G. lucidum. To isolate active single compound from G. lucidum (GASC), we conducted ethanol extraction and chloroform fractionation. In addition, we assayed the inhibitoty effect of tyrosinase activity and melanin biosynthesis in B16F10 mouse melanoma cells. In the present study, we identified a GASC, which exhibited inhibitory effects of cellular tyrosinase activity, the protein expression of cellular tyrosinase and tyrosinase-related protein-1, 2. In additional, microphthalmia-associated transcription factor (MITF), as known as crucial regulator of melanogenesis-related genes, was down-regulated by treatment with GASC in a concentration-dependent manner. GASC exhibited significant inhibition of tyrosinase activity and melanin synthesis in B16F10. The finding that melanogenesis inhibitory effect of GASC will contribute to facilitate various approaches of this mushroom for use in skin whitening products.

In our previous study, active-single compound (ASC), inhibitor of melanogenesis in B16F10 melanoma cell, was identified from Ganoderma lucidum. Thus, the aim of this study is to analyze the change of the ASC contents in G. lucidum when oriental raisin tree (ORT) was added in cultivation of G. lucidum. In HPLC analysis, significantly increased ASC peak was observed in G. lucidum extract supplemented with 15% (v/v) of ORT. In addition, melanogenesis inhibitory effect of G. lucidum extract supplemented with 15% of ORT significantly increased when compared to G. lucidum extract without ORT supplementation. Furthermore, ORT supplementation increased mycelial growth of G. lucidum in both solid and liquid cultivation. These results suggest that oriental raisin tree is useful as natural ingredient for increasing bioactivity including skin-whitening effect and mycelial growth of G. lucidum.

본 연구에서는 고체추진기관의 2차원 축대칭 FSI 해석에서 CSS 및 ISS 기법의 수치 안정성을 고찰하였다. 이를 위하여 CSS 및 ISS 기법을 2차원 축대칭 FSI 수치해석 알고리즘에 구현한 프로그램을 작성하고, 이를 ACM 및 BCM 고체추진기관의 복합거동 해석에 사용하였다. 해석 결과들을 비교 분석하여 ISS 기법이 고체추진기관 FSI 해석의 수치 안정성 개선에 효과적인지 검토하였다. 연구결과, ISS 기법을 적용한 FSI 해석은 시스템 시간간격이 작아질수록 수치적 수렴성을 보이며, CSS 기법과 다르게 시간이 진행되어도 수치해의 진동이 발산하지 않는 것을 확인할 수 있었다. 이를 통하여 ISS 기법을 사용하면 ACM 및 BCM의 FSI 해석에 CSS 기법을 이용할 시 나타나는 수치 불안정성을 개선할 수 있음을 확인하였다.