Gryllus bimaculatus is one of many cricket species known as field crickets. Also known as the African or Mediterranean field cricket or as the two-spotted cricket, it can be discriminated from other Gryllus species by the two dot-like marks on the base of its wings. G.bimaculatus is a subtropical insect and widely distributed from Africa to south Asia. After into the country, The species are popular for use as a food source for insectivorous animals like spiders and reptiles kept as pets. In 2016, G.bimaculatus was approved as a general food ingredient by Korean Ministry of Food and Drug Safety. However, domestic research on G.bimaculatus is still in its study is beginning stages. G.bimaculatus is possible species to year-round rearing without storage condition. but The aim of the present study prepares for in case of problems such as breeding space, labor cost etc. In the laboratory condition at 28±2℃ and 50% relative humidity under 10h light, 12h dark photoperiod, Adult crickets oviposit at soaked flower foam for 24 hours. The experiment on the hatching of the eggs showed that eggs could be stocked at 16℃ for 10 days with 7 day pre-period after laying, representing 85% hatchability.

아메리카왕거저리의 사육기간을 단축과 기능성 향상을 위해 밀기울에 다양한 먹이를 보조사료로 공급하여 발륙기 간과 무게를 측정한 결과, 먹이종류별 아메리카왕거저리의 발육기간은 조단백질함량이 많은 배합사료와 밀기울에서 80.8일과 75.6일로 가장 짧았으며 버섯배지는 160일이상 소요되었으며 왕겨의 생충율은 85%로 가장 낮아 버섯폐배지 와 왕겨는 먹이원으로 적절하지 않았다. 보조 먹이원 선발을 위해 할맥, 압맥, 배추, 백련초, 파프리카, 개똥쑥을 20~80% 밀기울에 첨가한 결과 압맥과 할맥은 정상적으로 생장하지만 백련초, 개똥쑥 혼합에서는 접종 직후부터 죽은 개체들이 출현하여 생충율이 12.9%, 10.8%로 아주 낮게 나타났으며 배추와 파프리카도 24.2~38.8%로 낮아 보조사료로 적합하지 않았다. 들깨와 콩가루 역시 생충율이 높지 않아 1, 2차 시험결과 할맥, 압맥과 메밀이 보조사료로 적합하였다.

Alcoholic steatosis is a fundamental metabolic disorder and may precede the onset of more severe forms of alcoholic liver disease. In this study, we isolated enzymatichydrolysate from Semisulcospira libertine by alcalase hydrolysis and investigated the protective effect of Semisulcospira libertine hydrolysate on liver injury induced by alcohol in the mouse model of chronic and binge ethanol feeding (NIAAA). In an in vitro study, the hydrolysate protects HepG2 cells from ethanol toxicity. Liver damage was assessed by histopathological examination, as well as by quantitating activities of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). After the administration of S. libertina hydrolysate, fat accumulation and infiltration of inflammatory cells in liver tissues were significantly decreased in the NIAAA mouse model. The elevated levels of serum AST, ALT, and ALP activities, along with the lipid contents of a damaged liver, were recovered in experimental mice administrated with S. libertina hydrolysate, suggesting its role in blood enzyme activation and lipid content restoration within damaged liver tissues. Moreover, treatment with S. libertine hydrolysate reduced the expression rate of cyclooxygenase (COX-2), interleukin (IL)-1β, and IL-6, which accelerate inflammation and induces tissue damage. All data showed that S. libertine hydrolysate has a preventive role against alcohol-induced liver damages by improving the activities of blood enzymes and modulating the expression of inflammation factor, suggesting S. libertine hydrolysate could be a commercially potential material for the restoration of hepatotoxicity.