Alcoholic steatosis is a fundamental metabolic disorder and may precede the onset of more severe forms of alcoholic liver disease. In this study, we isolated enzymatichydrolysate from Semisulcospira libertine by alcalase hydrolysis and investigated the protective effect of Semisulcospira libertine hydrolysate on liver injury induced by alcohol in the mouse model of chronic and binge ethanol feeding (NIAAA). In an in vitro study, the hydrolysate protects HepG2 cells from ethanol toxicity. Liver damage was assessed by histopathological examination, as well as by quantitating activities of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). After the administration of S. libertina hydrolysate, fat accumulation and infiltration of inflammatory cells in liver tissues were significantly decreased in the NIAAA mouse model. The elevated levels of serum AST, ALT, and ALP activities, along with the lipid contents of a damaged liver, were recovered in experimental mice administrated with S. libertina hydrolysate, suggesting its role in blood enzyme activation and lipid content restoration within damaged liver tissues. Moreover, treatment with S. libertine hydrolysate reduced the expression rate of cyclooxygenase (COX-2), interleukin (IL)-1β, and IL-6, which accelerate inflammation and induces tissue damage. All data showed that S. libertine hydrolysate has a preventive role against alcohol-induced liver damages by improving the activities of blood enzymes and modulating the expression of inflammation factor, suggesting S. libertine hydrolysate could be a commercially potential material for the restoration of hepatotoxicity.

Domestic bitches are non-seasonally monoestrus; spontaneously ovulate only once or twice occurs at anytime of the year. Estrus induction has been applied infrequent estrus, misleading ovulation, mating difficulties, failure to conceive after normal mating, pregnancy failure and biological research. Protocol of estrus induction which included variable hormones such as FSH, GnRH, and PMSG have been applied for the last decades. Recently, Bromocriptine, one of anti-prolatin/dopamine agonist has been occasionally applied for estrus induction. The study was carried out to investigate the effective method for the induction of estrus in bitches using different hormone treatments, and the initiation time of estrus from hormone treatment by assessments of cytological observation and blood plasma progesterone concentration. A total of 54 bitches on anestrus were selected for the study and divided randomly into 8 treatment groups as follow. Control, natural estrus; FSH (L), FSH (1.5 mg/kg, twice a day, , Vetrepharm); FSH (H), FSH (3.0 mg/kg, twice a day); GnRH+FSH, GnRH (5 ug/kg, once first day, , Dongbang)+FSH (3 mg/kg, SID); PMSG, PMSG (50 IU/kg, every third day , Intevet); GnRH+PMSG, GnRH (5 ug/kg, only first day)+PMSG (50 IU/kg, every third day); GnRH, GnRH (5 ug/kg, only first day); Bromocriptine, bromocriptine (0.3 mg/kg, SID, , Novartis). The bitches were evaluated clinical sign, cytological exam and Premate for assessment of estrus induction. Estrus induction rates were significantly (P<0.05) higher in GnRH+PMSG (100%) compared to others. PMSG and GnRH+PMGS (87.5 and 100%) and Bromocriptine (77.8%) were higher than others except GnRH+PMSG. Analysis of vaginal smear has proved to be effective a correct assessment of estrus induction with assay of progesterone concentration by Premate. Proestrus initiated by the after induction in most case. In conclusion, bromocriptine is an effective drug for estrus induction in bitches and assay of progesterone concentration by Premate with examination of vaginal smear that should be useful to detection of estrus induction of estrus induced bitches.

In dogs, correct diagnosis of estrus is important and the exact time of ovulation can be determined by variouse methods. Vaginal cytology has commonly used in conjunction with the physical examination, clinical history, vaginoscopy, and hormonal assays to determine the stage of the reproductive cycle. This study was therefore investigated the effectiveness of direct ovulation detector designed by changes of electrical resistance in vaginal mucus following different estrus cycles with several methods; vaginal cytology, concentration of plasma estrogen and progesterone, and direct examination by laparotomy. A total of 12 bitches was selected for the study and observed estrus signs. The bitches were evaluated clinical sign (vulvar swelling and bleeding), cytological examination (keratocyte and RBC), electrical resistance, plasma estrogen and progesterone concentration for estrus assessment. Accuracy of ovulation detection by vaginal cytology was significantly (p<0.05) lower than those by electrical resistance and plasma progesterone concentration, based on the confirmation by laparotomy. Vaginal smear is not confidential method compared to detection of electrical resistance and plasma progesterone concentration at ovulation. Although the value of electrical resistance was varied at the same points of estrus in individuals, ovulation was occurred at the first day which shown the peak of electrical resistance and mating time was third day after peak. In conclusion, ovulation detector designed by changes of electrical resistance is an effective and economic instrument for predicting estrus and ovulation in bitches.

개 난자의 체외성숙율을 높이기 위하여 다양한 방법들이 시도되고 있지만 여전히 그 효율성은 낮다. 본 연구는 개 난자의 체외성숙 시, 성선 자극 호르몬인 황체형성호르몬(LH)과 난포자극호르몬(FSH), 상피세포성장인자(Epidermal growth factor, EGF) 그리고 시스테인(cysteine)을 각각 첨가하여 72시간 동안 체외성숙시킨 후 핵성숙율(GV: germinal vesicle, GVBD: germinal vesicle break down, MI: metaphase I, MII: metaphase II, UK: unknown stage)을 확인하였다. LH와 FSH를 첨가하였을 때 첨가하지 않은 군과 GV, MI 및 MII율에는 유의적인 차이는 없었다. 하지만 GVBD율은 첨가군이 유의적으로(p<0.05) 높았다. 성선 자극 호르몬을 첨가한 배지에 10 ng/ml의 EGF를 첨가하였을 때 MII율이 첨가하지 않은 군보다 유의적으로(p<0.05) 높았다(4.54% vs. 7.06%). cysteine을 첨가하였을 경우, 핵성숙율에 유의적인 차이는 보이지 않았지만 전반적으로 핵성숙율이 향상된 것을 확인할 수 있었다. 따라서 개 난자의 체외성숙 시, 10 μg/ml의 LH와 FSH, 10 ng/ml의 EGF 그리고 0.57 mM의 cysteine을 첨가하는 것이 핵성숙율을 향상시키는 것으로 사료된다.

본 연구는 수석교사제 법제화의 내용과 쟁점을 살펴보고, 교사들과의 면담을 통해 현재 실시되고 있는 수석교사제의 적용 상황, 문제점을 파악한 뒤 현창 착근을 위한 개선 방안을 제시하는 데 목적이 있다. 선행연구를 통해 제기된 수석교사제 관련 법령 쟁점 사항으로는 이원화 체제와 관련된 수석교사제 도입 목적의 문제, 수석교사의 위상과 역할 문제, 수석교사 자격요건과 선발기준 문제가 있었다. 제주특별자치도 초등학교 수석교사와 일반교사 8명을 심층 면담한 후, Colaizzi의 자료분석 방법에 의해 수석교사제 관련 경험을 들여다 본 결과, 법제화의 힘, 첫 단추의 스트레스, 이원화 취지에 맞게, 경쟁력 있는 수석교사라는 4개의 주제로 정리할 수 있었다. 수석교사제 관련 쟁점과 교사 면담을 통해 제기된 문제들에 대한 개선 방안으로 수석교사 지위에 대한 합의, 수석교사의 질 강화 노력, 업무 규정과 세부 매뉴얼 제작, 일반교사들을 대상으로 한 연수 및 홍보 확대를 제시하였다.

월동 배추 3품종 중 외형적 특징을 조사한 결과 '만풍'이 가장 크고, 무거웠으며 잎의 두께도 가장 두꺼웠다. 그러나 김치제조 시 다듬는 과정에서 초록색의 크고 두꺼운 겉잎이 많이 제거되어 가식부위의 무게는 '설왕'보다 적었다. 배추 3품종 중잎이 두꺼운 '만풍'이 경도 또한 가장 높았으며 산도도 높았다. 김치 제조 후 품질을 조사한 결과 '만풍'은 조직의 경도 저하가 다른 품종에 비하여 적었지만 pH 및 총산의 변화에 있어서는 '만풍' 품종이

배추의 V/H ratio는 PE film으로 포장한 배추가 포장하지 않은 control에 비해 더 높게 유지되고 있으며, 속잎이 겉잎에 비해 V/H ratio가 더 높게 유지되었다. Pouch내의 의 농도는 처리구 모두 15일 정도까지는 량이 급격히 증가하고 그 이후부터는 거의 일정한 경향을 보였다. pouch내의 농도는 저장기간이 길어짐에 따라 15일까지 급격히 감소하다가 그 후에는 변화량이 적었다. 의 농도는 와 반대로 film의 두께가 얇