The nitrogen isotope value in both ammonium and nitrate ion were determined at 9 stations during both June and August 2016, in order to understand the origin of DIN at the Han river. δ15N-NO3 and δ15N-NH4 values in 8 stations (CP, SB, MHC, P4, SJ, SBC, P2, SC) were no significant variation. However δ15N-NO3 and δ15N-NH4 values in KK (Kyeongan stream) showed significant different in comparison with 8 stations, with an apparent increase of nitrogen isotope values. These results indicate that antropogenic nitrogen source influence on KK station. Also the δ13C and δ15N isotope ratio of phytoplankton (Diatom and Cyanobacteria) in KK (Kyeongan stream) showed heavier values, compared to other study stations. These results indicate that nitrogen isotope value in phytoplankton effects by different nitrogen source in study sites. These results suggest that the analysis of stable isotope ratios is a simple but useful tool for the identification of dissolved inorganic nitrogen origin in aquatic environments.

This study was conducted for recycling the waste MDF(Medium-density fibreboard) to investigate the enzymatic saccharification characteristics using two enzymes Novozyme Cellic® CTec2 and HTec2 (Novozymes, Bagsvaerd, Denmark) after the delignification by pretreatment using sodium chlorite. The chemical composition of the waste MDF are lignin, holocellulose, ash, and other extracts 28.40, 60.20, 0.10, and 11.30%, after pretreatment with sodium chlorite were 5.20, 53.10, 0.03, and 41.67%. The Lignin interferes with enzymatic saccharification of 23.2% was removed, 7.1% of holocellulose was lost. The times of sodium chlorite pretreatment and saccharification of the waste MDF was finished between 48-72 hours, the saccharification speed was fast when the concentration of the enzyme by 10% and the HTec2 CTec2 ratio 9:1. Sugar ratio of the solid content of the waste MDF is the highest as 69.6% when it comes out of 8% and a viscosity was as high as 34.8% when the 12-FB%. Therefore, the pre-treatment with sodium chlorite is more advantageous when enzymatic saccharification to lignocellulosic biomass. The amount of the enzyme, the solid-liquid ratio, and the reaction time showed a proportional relationship with saccharification efficiency. The studies for increasing the solids content of waste MDF to improve the economic efficiency more than 12% should accompanied.

본 연구는 곤충 병원성 균주 Beauveria bassiana 배양액을배양일 수에 따라 이화학적 특성, 항산화 활성, 항염증활성 및항암활성을 검정하여 천연 생리활성 물질 및 기능성 신소재개발을 위한 기초자료를 얻고자 하였다.1. 유리당은 Glucose, Maltose, Fructose 존재하였고 Glucose함량이 B. bassiana 15.2g·L−1으로 배양 3일 시 다량 함유되어 있었으며 배양기간이 길어질수록 함량이 감소하였다. 2. 유기산은 Citric acid, Malic acid, Formic acid, Lacticacid, Acetic acid, Succinic acid 총 6종의 성분이 동정되었고,Citric acid 성분이 다량 함유되었으며 균주 중 B. bassiana.3일 배양액이 276.4mg·L−1로 가장 많이 검출되었음.3. B. bassiana 균주 배양액은 총 16종의 아미노산으로 조성되어 있었으며 Glutamate 함량이 배양 3일 시 987.0pmol/µl으로 다량 함유되어 있었다.4. 무기성분은 Na이 다량 함유되어 있었으며 B. bassiana 15일 배양액이 179.5mg·L−1로 가장 많은 Na을 함유하고 있었다.5. B. bassiana 배양 5일 시 6.34±0.30ppm, 플라보노이드함량은 B. bassiana 배양 3일 시 1.09±0.05ppm, 배양 15일시 1.10±0.06ppm으로 높은 함량을 나타냈다.6. DPPH free radical 소거능은 B. bassiana 배양 15일 시263.4±13.3µg·ml−1, SOD 활성은 B. bassiana 배양 15일 시93.7±16.6µg·ml−1, ABTS 활성은 B. bassiana 배양 5일 시99.7±1.2µg·ml−1으로 높은 활성이 나타났다.7. Anticancer activity 결과 A549 cell lines은 B. bassiana5일 배양액(163.8±4.1µg·ml), HepG2 cell lines는 B. bassiana10일 배양액(127.8±2.3µg·ml)으로 항암활성이 높게 나타났으며, MCF-7 cell lines에 검정한 결과, B. bassiana 5일 배양액(129.1±6.8µg·ml)으로 높은 활성을 나타냈으나 대조구인Doxorubicin보다는 낮은 활성이 나타났다.

This study was conducted to investigate the biomedicinal worth of different parts of Geranium sibiricum Antioxidant, anti-inflammatoty and anticancer activities of methanolic extracts of root and shoot of Geranium sibiricum were determined. Methanolic extracts of root and shoot parts of Geranium sibiricum had antioxidant activity in the range of 40-50 jlg mL·1, and shoot extract was best in this regard. Likewise, anti-inflammatory activities of extracts was in the range of 60-100 jlg mL-1 and root extract of Geranium sibiricum seemed more useful with 87.38% higher anti-inflammatory activity than shoot with 63.63% activity. Regarding cytotoxicty test against two cancer cell line i.e. SK-Hep1 (liver) and HeLa (uterus), the methanolic extracts of shoot and root of Geranium sibiricum showed the highest toxicity with IC50 values of 10-30 jlg mL-1 and root extract proved more beneficial in this regard. In conclusion, methanolic extract of shoot of Geranium sibiricum had higher antioxidant while root extract had higher anti-inflammatoty and anticancer activities.

Sediment discharge by long-term runoff in the Nakdong River watershed should be predicted for the maintenance and management of the Nakdong River newly changed by the four major river restoration project. The data establishment by the analysis of runoff and sediment discharge using the long-term watershed model is necessary to predict possible problems by incoming sediments and to prepare countermeasures for the maintenance and management. Therefore, sediment discharges by long-term runoff in the main points of the Nakdong River were calculated using SWAT(soil and water assessment tool) model and the relations and features between rainfall, runoff, and sediment discharge were analyzed in this study. As a result of sediment discharge calculation in the main points of the Nakdong River and tributaries, the sediment discharge at the outlet of the Naesung Stream was greater than the Jindong Station in the Lower Nakdong River from 1999 to 2008 except the years with low precipitation. The sediment discharge at the Nakdong River Estuary Barrage (NREB) was corresponding to 20% of the Jindong Station which is located about 80 km upstream from NREB.