Fluorescent bacteria were isolated from sporocarps that browned into various mushrooms during survey at places of the production in Korea. We examined the pathogenicity, biodiversity, and genetic characteristics of the 19 strains identified as Pseudomonas tolaasii by sequence analysis of 16S rRNA and White Line Assay. The results emphasize the importance of rpoB gene system, fatty acid profiles, specific and sensitive PCR assays, and lipopeptide detection for the identification of P. tolaasii. As a result of these various analyses, 17 strains (CHM03~CHM19) were identified as P. tolaasii. The phylogenetic analysis based on the 16S rRNA gene showed that all strains were clustered closest to P. tolaasii lineage, two strains (CHM01, CHM02) were not identified as P. tolaasii and have completely different genetic characteristics as a result of fatty acids profile, specific and sensitive PCR, lipopetide detection, rpoB sequence and REP-PCR analysis. Pathogenicity tests showed 17 strains produce severe brown discolouration symptoms to button mushrooms and watersoaking of sporophore tissue within three days after inoculation. But two strains did not produce discolouration symptoms. Therefore, these two strains will be further investigated for correct species identification by different biological and molecular characteristics.
Global warming and changes in ecosystems are either causing a rapid decline in insect diversity on earth or, on the other hand, are increasing the likelihood of unexpected insect pests emerging. This study summarizes and reports the followings: Introduction to the Insects of Red Data Book of Korea and Nationally Protected species, the establishment of monitoring strategy for exotic insects by the investigation of species distribution range through field surveys and others, the dispersal of exotic insects, and with the expansion of exotic insects and development of ecosystem impact prediction model.
토복령은 우수한 항균, 항산화, 항염증 효능을 가진 소재로 알려져 있다. 이러한 토복령(Smilax china)의 추출물의 기능성을 화장품에 적용하기 위한 기초연구로써 토복령에서 발견되는 플라보노이드인 quercetin, catechin, naringenin의 농도별 경피 투과 특성을 조사할 필요성이 있다. Marzulli의 정의에 적 용한 결과 케르세틴의 Kp 값은 0.1 mg/mL에서 "빠름"으로 분류되었고, 0.2 및 0.4 mg/mL에서 "보통"으로 분류되었다. 특히, 농도가 증가함에 따라 투과 속도가 감소하는 경향이 있었다. 나린제닌의 경우 Flux 값은 각각 0.1, 0.2 및 0.4 mg/mL 농도에서 0.69, 1.07 및 1.42 μg/hr/cm²이었으며, 해당 Kp 값은 각각 6.95, 5.34 및 3.56이었다. 나린제닌의 Kp 값은 모든 농도에서 "보통" 범주에 속하며, 케르세틴과 관찰된 것과 같이 농도가 높아짐에 따라 투과 속도가 감소하였다. 카테킨의 경우 Flux 값은 각각 0.1, 0.2 및 0.4 mg/mL 농도에서 0.75, 1.09 및 1.66 μg/hr/cm²이었으며, 해당 Kp 값은 각각 7.55, 5.46 및 4.16이었다. 카테킨의 Kp 값은 모든 농도에서 일관되게 "보통"으로 분류되었다. 여드름 저해능 및 항염증 효능이 우수 한 토복령 추출물의 유효성분인 quercetin, catechin, naringenin의 경피 투과 특성이 보통 이상으로 나타나 기능성 화장품에 사용할 수 있는 우수한 천연물 소재인 것을 확인할 수 있었다.
The inorganic scintillator used in gamma spectroscopy must have good efficiency in converting the kinetic energy of charged particles into light as well as high light output and high light detection efficiency. Accordingly, various studies have been conducted to enhance the net-efficiency. One way to improve the light yield has been studied by coating scintillators with various nanoparticles, so that the scintillation light can undergo resonance on surface between scintillators and nanoparticles resulting in higher light yield. In this study, an inorganic scintillator coated with CsPbBr3 perovskite nanocrystals using dip coating technique was proposed to improve scintillation light yield. The experiment was carried out by measuring scintillation light output, as the result of interaction between inorganic scintillator coated with CsPbBr3 perovskite nanocrystals and gamma-ray emitted from Cs-137 gamma source. The experimental results show that the channel corresponding to 662 keV full energy peak in the Cs-137 spectrum shifted to the right by 14.37%. Further study will be conducted to investigate the detailed relationships between the scintillation light yield and the characteristics of coated perovskite nanoparticles, such as diameter of nanoparticles, coated area ratio and width of coated region.
In the case of nuclear projects, when developing a new reactor type, it is necessary to confirm the reactor type, secure the safety, and especially obtain the construction permit approval of the licensing authority for construction. Schedule management is necessary to carry out nuclear projects, and progress rate management of project progress management is largely composed of three elements: scope management, cost management, and resource management. However, in the case of the small modular reactor (SMR) project currently being carried out, it is difficult to calculate the progress rate including budget and resources due to the nature of the project. Therefore, in the SMR project, it took two years from the beginning to prepare the integrated project master schedule (IPMS) to prepare the draft, and then two revisions were made over a year and a half. In this SMR project, we will consider the entire construction period such as design, purchase and production, construction, commissioning, and operation in terms of scope management. The entire document list was created using the document review and approval sheet created at the beginning of the design. In the PMIS (Project Management Information System), the number of approved documents was calculated by comparing the list of engineering documents. In the purchase production part, the main core equipment such as the primary system nuclear steam supply system (NSSS), the secondary system turbine and condenser, and the man machine interface system (MMIS) are managed. Purchasing and manufacturing management shall be managed so that major equipment can be delivered in a timely manner in accordance with the schedule for delivery of equipment in the IPMS. In order to prevent delays in the start of production, it is necessary to minimize the waiting time for work through advance management tasks such as insurance of drawing, stocking of materials, availability of production facilities, etc. In this way, we decided to carry out the schedule management for the design, purchase and manufacturing part in the SMR project first, and the installation, construction and commissioning part will be prepared for the future schedule management.
Understanding the dispersion of xenon isotopes following a nuclear test is critical for global security and falls within the remit of both the Comprehensive Nuclear-Test-Ban Treaty (CTBT) and the International Noble Gas Experiment (INGE). This paper aims to show if it is possible to discriminate the source of xenon releases based on the atmospheric dispersion of xenon isotopes using HYSPLIT. Using ORIGEN and SERPENT simulations, four released scenarios are defined with four different fractionation times (i.e., 1 hour, 1 day, 10 days, and 30 days) after a 1kt TNT equivalent 235U explosion event. These time-delayed release scenarios were selected to certify the possibility of mis-determining xenon release source. We use the Lagrangian dispersion model for atmospheric dispersion to predict the concentration distribution of xenon isotopes under each scenario. The model allows us to better understand how these isotopes would distribute over time and space, offering valuable data for real-world detection efforts. To our knowledge, there have been no researches on the analysis of xenon isotopic ratios considering atmospheric dispersion. In this work, we focused on the atmospheric dispersion using HYSPLIT to characterize the xenon isotopic ratios from nuclear tests. In addition, we compared the xenon isotopic ratios obtained from the atmospheric dispersion with those from ORIGEN calculations, which would be helpful to discriminate the source of the xenon releases.
Recently, as the possibility of unexpected outbreaks of alien insects has increased due to climate change such as global warming, the importance of early control through rapid and accurate spread of exotic forest pest and change prediction diagnosis is required. This study summarizes and reports the followings: the establishment of monitoring strategy for exotic insects by the investigation of species distribution range through field surveys and others, the development of new diagnostic technique through microstructures and life-cycle, the dispersal of exotic insects, and ecological impact assessment using ecological methods and with the expansion of exotic insects and development of ecosystem impact prediction model.
In this study, we investigate the opportunity of using waste tire char as a cathode material for lithium-ion primary batteries (LPBs). The char obtained by carbonizing waste tires was washed with acid and thermally fluorinated to produce CFX. The structural and chemical properties of the char and CFX were analyzed to evaluate the effect of thermal fluorination. The carbon structure of the char was increasingly converted to CFX structure as the fluorination temperature increased. In addition, the manufactured CFX- based LPBs were evaluated through electrochemical analysis. The discharge capacity of the CFX reached a maximum of 800 mAh/g, which is comparable to that of CFX- based LPBs manufactured from other carbon sources. On the basis of these results, the use of waste tire char-based CFX as a cathode material for LPBs is presented as a new opportunity in the field of waste tire recycling.
The organic complexing agents such as ethylenediaminetetraacetic acid (EDTA), nitrilotriacetic acid (NTA), and isosaccharinic acid (ISA) can enhance the radionuclides’ solubility and have the potential to induce the acceleration of radionuclides’ mobility to a far-field from the radioactive waste repository. Hence, it is essential to evaluate the effect of organic complexing agents on radionuclide solubility through experimental analysis under similar conditions to those at the radioactive waste disposal site. In this study, five radionuclides (cesium, cobalt, strontium, iodine, and uranium) and three organic complexing agents (EDTA, NTA, and ISA) were selected as model substances. To simulate environmental conditions, the groundwater was collected near the repository and applied for solubility experiments. The solubility experiments were carried out under various ranges of pHs (7, 9, 11, and 13), temperatures (10°C, 20°C, and 40°C), and concentrations of organic complexing agents (0, 10-5, 10-4, 10-3, and 10-2 M). Experimental results showed that the presence of organic complexing agents significantly increased the solubility of the radionuclides. Cobalt and strontium had high solubility enhancement factors, even at low concentrations of organic complexing agents. We also developed a support vector machine (SVM) model using some of the experimental data and validated it using the rest of the solubility data. The root mean square error (RMSE) in the training and validation sets was 0.012 and 0.016, respectively. The SVM model allowed us to estimate the solubility value under untested conditions (e.g., pH 12, temperature 30°C, ISA 5×10-4 M). Therefore, our experimental solubility data and the SVM model can be used to predict radionuclide solubility and solubility enhancement by organic complexing agents under various conditions.