This study investigates the factors influencing the seed longevity of Quercus myrsinifolia, a species with recalcitrant seeds highly sensitive to desiccation and freezing. The effects of moisture content, seed collection date, and storage methods on seed viability were analyzed using exponential decay modeling. Interactions between these factors were also explored to refine conservation strategies. Seeds with moisture content above 40% demonstrated a predicted seed longevity of 2.19 years, whereas those with moisture content below 30% had seed longevity of less than 1 year. Late-season seeds collected in November and December exhibited superior germination percentages and longer predicted seed longevity (1.32 years) compared to early-season seeds collected in September and October (<1 year). In seed weight, large seeds (2.0 g) showed longer predicted seed longevity about 1.5 times greater than that of small seeds (<1.2g). Storage methods significantly affected seed longevity, with refrigerator (4°C) with silica gel maintaining viability for 2–3 years, while seeds stored at room temperature (25°C) exhibited a seed longevity of less than 1 year. Silica gel was found to prevent seed deterioration due to over-desiccation, emphasizing the importance of balanced moisture regulation. Q. myrsinifolia seeds exhibited 𝑏 values ranging from 0.30 to 2.04, demonstrating a close relationship between decay constant, moisture content, storage conditions, and seed longevity. These findings provide critical insights into optimizing seed storage and propagation strategies for Q. myrsinifolia, contributing to its conservation and ecological restoration efforts.

Background: With the growing interest in the health of companion dogs, their average lifespan has increased, leading to an increase in the proportion of elderly dogs. As elderly dogs are vulnerable to various diseases, there is a need for alternatives to predict the risk of major diseases in senior dogs, prevent them in advance, and manage their health effectively. Therefore, this study was conducted to identify candidate genes and single nucleotide polymorphisms (SNPs) influencing primary angle-closure glaucoma, a major disease in elderly dogs, using the Axiom Canine HD Array and establishing foundational data. Methods: Samples from 95 dogs of 26 breeds from South Korea were analyzed using an SNP chip. Ultimately, two SNPs were selected. To assess the impact of non-synonymous SNP (nsSNPs), functional analysis of candidate genes, Hazard Assessment, and protein structure prediction were conducted. Sequencing for SNP validation involved samples from 95 dogs of ten breeds with reported domestic and international glaucoma cases. Results: The candidate gene TNS1 was associated with the integrin signaling pathway. The selected nsSNP was found to cause a mutation at the ninth position of the amino acid sequence, changing serine to leucine and resulting in alterations to the overall protein structure. Sequencing analysis results for SNP validation revealed differences in frequency among breeds. Conclusions: The identified SNP markers are potential risk prediction tools. Utilizing genotype frequency data by breed and individual could aid in disease management and contribute to advancements in the medical industry.

This study investigated the morphological characteristics and regional variations of leaves, flowers, and seeds of Quercus myrsinifolia Blume to understand its ecological adaptation and the effects of environmental factors. Samples were collected from Jinju, Hapcheon, and Sancheong, and nine leaf traits, six flower traits, and five seed traits were analyzed. Significant regional variations were observed, with Hapcheon exhibiting the largest leaf and flower sizes, while Sancheong showed the largest and heaviest seeds. Jinju recorded the smallest values for most traits. Principal Component Analysis (PCA) revealed distinct regional groupings, with Hapcheon displaying intermediate traits, Sancheong larger traits, and Jinju smaller traits. Correlation analysis identified strong positive relationships between leaf length and width, seed length and weight, and the number of staminate flowers and catkin width, highlighting key indicators for growth. Climate factors such as temperature and precipitation significantly influenced morphological traits, with higher temperatures negatively affecting leaf and seed sizes, while precipitation showed a weak positive correlation with seed weight. Among soil factors, pH and magnesium content were closely related to morphological traits. pH exhibited a negative correlation with leaf length and petiole length, while magnesium showed a positive correlation with seed weight and leaf width. These findings underscore the significant role of environmental factors in morphological variation and provide valuable insights for developing regionally adaptive breeding strategies. These findings provide foundational data for developing region-specific breeding strategies and cultivars for Q. myrsinifolia, contributing to ecological management and climate change adaptation strategies.

To improve the lithium-ion battery performance and stability, a conducting polymer, which can simultaneously serve as both a conductive additive and a binder, is introduced into the anode. Water-soluble polyaniline:polystyrene sulfonate (PANI:PSS) can be successfully prepared through chemical oxidative polymerization, and their chemical/mechanical properties are adjusted by varying the molecular weight of PSS. As a conductive additive, the PANI with a conjugated double bond structure is introduced between active materials or between the active material and the current collector to provide fast and short electrical pathways. As a binder, the PSS prevents short circuits through strong π‒π stacking interaction with active material, and it exhibits superior adhesion to the current collector, thereby ensuring the maintenance of stable mechanical properties, even under high-speed charging/discharging conditions. Based on the synergistic effect of the intrinsic properties of PANI and PSS, it is confirmed that the anode with PANI:PSS introduced as a binder has about 1.8 times higher bonding strength (0.4 kgf/20 mm) compared to conventional binders. Moreover, since active materials can be additionally added in place of the generally added conductive additives, the total cell capacity increased by about 12.0%, and improved stability is shown with a capacity retention rate of 99.3% even after 200 cycles at a current rate of 0.2 C.

Hydrogen peroxide (H2O2) is widely used in bleaching treatments in the pulp and paper industry, in wastewater treatment, and as a food additive. However, H2O2 solutions are unstable and decompose slowly when subjected to external factors such as light, high temperatures, or metal compounds. Therefore, a simple and reliable method to measure the concentration of H2O2 is required for its proper use in various applications. We determined the concentration of an H2O2 solution by measurement at a single wavelength (249 nm) without any reagents or complex analytical procedures. In the present work, the measurable concentration of H2O2 was as low as 0.015 wt% (4.41 mM) and as high as 0.300 wt% (88.2 mM), with high linearity (99.99% at 249 nm) between the concentration of H2O2 and the optical density (OD) values. In addition, the method could be used to measure the concentration of H2O2 in a peracetic acid solution without interference from acetic acid and peracetate ion.

국립원예특작과학원에서는 밝은 화색과 안정적인 화형의 생 육이 우수한 빨간색 스탠다드 장미 품종을 육성하기 위해 진한 적색 스탠다드 장미 품종 ‘엔드리스러브(Endless Love)’를 모 본으로, 꽃잎수가 많고 안정적으로 가시가 적은 밝은 노란색 ‘페니레인(Penny Lane)’ 품종을 부본으로 인공교배하였다. 37 개의 교배실생을 양성해 1, 2, 3차에 걸친 특성검정 및 현장실증 을 통해 꽃이 크고 화형이 안정적이며, 재배안정성 및 생산성, 절화특성이 우수한 ‘원교 D1-390’을 최종 선발하였다. 2023년 ‘루비레드(Ruby Red)’로 명명하여 국립종자원에 품종보호출원·등록되었다. ‘루비레드’ 품종은 밝은 적색(R53C)을 가졌으 며, 꽃잎수가 32.8매, 화폭과 화고는 각각 10.9, 5.9cm로 대조 품종보다 크다. 절화장은 평균 71.7cm, 절화수명은 약 16.7일, 수량은 연간 168대/m2로 대조품종인 ‘레드스퀘어(Red Square)’ 대비 절화장이 길고 절화수명도 2배 이상 길며, 수확량도 1.4배 우수하다. 2023년 국내 육성 장미 품종 서울식물원 관람객 대상 공동평가회에서 스탠다드 장미 중 우수한 평가를 받았으며, 현 장 실증 결과 농가별로 균일하고 우수한 수량과 절화품질을 보 였다. 절화용 장미 ‘루비레드’ 품종은 밝은 적색과 우수한 화형 을 가지는 품종으로 해외 대체 품종으로 국내에서 많이 재배될 것으로 기대된다.

Helicobacter pylori are known as a causative agent of gastritis, gastric duodenum and peptic ulcer, and gastric cancer, and multiple drug use is associated with various side effects in patients. The discovery of antibacterial substances against H. pylori from Korean resource plants is an important substitute for antibiotics. 52 species of Korean resource plants were collected and extracted with 50% ethanol, and antibacterial activity against H. pylori was measured using the disk diffusion method. The toxicity of plant extracts to human gastric adenocarcinoma(AGS) cells was measured by MTT assay, and the level of IL-8 secreted when gastric epithelial cells were inoculated with H. pylori was measured. As a result of measuring the antibacterial activity of H. pylori, antibacterial activity was confirmed in 38 plant extracts. The plant species with the strongest antibacterial activity were Chrysanthemum indicum, Rheum rhabarbarum, Patrinia scabiosaefolia and Petasites japonicus. C. indicum was not cytotoxic to H. pyroli-infected AGS cells and showed anti-inflammatory effects. This study's results can be used to develop healthy, functional foods and medical materials.

This study aimed to develop the in vitro method using domestic commercial diets to estimate nutrient digestibility in dogs. The existing in vitro method were tested and compared with literature data to develop new in vitro method. The development of in vitro method progressed as follows: modification of pepsin solution to an activated form and supplementation with 1% lipase. All the in vitro method progressed to 4 hours of stomach simulation and 2 hours of small intestine simulation. In vivo digestibility was measured using the same diets as beagle dogs. The supplementation of lipase methods showed significantly improved (p < 0.05) DM, OM, and EE than the existing and modified pepsin solution methods. The correlation between in vitro and in vivo data in DM, OM, and EE digestibility was high (r2 = 0.889, 0.907, and 0.721, respectively), and the correlation between in vitro and in vivo data in CP and GE digestibility was medium (r2 = 0.681 and 0.536, respectively). The current in vitro method is similar to in vivo digestibility and helps potentially predict digestibility for dogs. In conclusion, this developed in vitro method suggests that it can help estimate the nutrient digestibility of dogs' diets without in vivo experiments.

국내 원예작물에서 화분매개곤충의 의존도는 해마다 증가하고 있다. 우리는 사과, 딸기 등 화분매개곤충의 의존도가 높은 주요 과수와 시설작물에 대하여 화분매개곤충의 사용기술을 개발하고 이를 현장에 적용하는 연구를 수행하고 있다. 2021년부터 2022년까지 딸기, 키위 등 5가지 주요 농작물에서 화분매개벌을 적용한 결과, 딸기에서 뒤영벌의 적용결과 기존 꿀벌과 통계적으로 같은 수준의 화분매개효과를 나타내었으며, 하우스에서 농약적용시 하우스 밖으로 벌통을 위치시키는 것이 방치보다 벌의 소실을 20% 더 감소시킬 수 있었다. 인공수분 에 의존하고 있는 씨없는 수박에서 수분수 식재와 꿀벌을 이용함으로 기존 벌 방사보다 16% 착과율을 향상시킬 수 있었다. 시설고추에서 꿀벌과 뒤영벌의 혼합사용시 기존 꿀벌 방사보다 고추 수량이 10% 향상되었고, 토마토 에서 660㎡당 뒤영벌의 봉군량을 1.5배 증가시 토마토 수량은 4.4% 향상되었다. 키위는 꿀벌로 기존의 인공수분 을 충분히 대체할 수 있었고, 인건비가 60%이상 절감되었다. 또한 현재 시판중인 30종의 살충제와 27종의 살균제 에 대하여 24시간내 반수치사를 보인 농약은 각각 살충제 6종와 살균제 4종 이었다. 이어서 2023년부터 참외, 멜론, 사과, 단감에 대한 현장적용연구가 진행되고 있다.

Bee traffic at the hive entrance can be used as an important indicator of foraging activity. We investigated patterns of honeybees and bumblebees near their hives as a basis for calculating bee traffic using the image deep learning. The flight pattern near the hive differed significantly according to bee at entering and leaving the hive. Honeybees mainly showed flight that changed flight direction more than once (69.5%), whereas bumblebees mainly performed straight flight (48.7%) or had a single turn (36.5%) in flight. When bees entered the hive, honeybees primarily showed one-turn or two-turn flight patterns(88.5%), and bumblebees showed a one-turn flight pattern (48.0%). In contrast, when leaving the hive, honeybees primarily showed a straight flight pattern (63.0%), and bumblebees primarily showed a straight or one-turn pattern (90.5%). There was a significant difference in flight speed according to the flight pattern. The speed of straight flight (0.89±0.47 m/s) was 1.5 to 2.1 times faster than flight where direction changed. Therefore, our results can help determine the capturing and recognizing the flying image of bees when calculating bee traffic by image deep learning.

시설재배 딸기의 화분매개에 꿀벌 사용이 보편화되어있지만 동양종(A. cerana) 꿀벌의 화분매개효과 연구는 미비한 실정이다. 따라서 본 연구는 딸기에서 동양종 꿀벌의 화분매개자로 가능성을 평가하기 위하여 비닐하우 스 딸기에서 서양종 꿀벌과 화분매개행동 특성을 비교하였다. 향후 봉군 수명과 착과된 딸기의 기형과율품질을 평가 할 예정이다. 서양종과 동양종 꿀벌은 10시 이후부터 활동량이 증가하고, 13시에 방화활동이 가장 활발하였 으며 이후부터 감소하는 일주행동 패턴을 보였다. 서양종과 동양종 꿀벌 활동성은 모두 온도, 조도, 자외선과 정의 상관관계를 나타냈으며 상대습도와는 부의 상관을 보였다. 특히 서양종 꿀벌은 온도, 조도, 자외선이 같은 수준의 상관(r=0.7)을 보이는 것에 반해, 동양종 꿀벌의 경우 온도와 습도보다 광조건(조도, 자외선)에서 더 높은 상관계수(r＞0.9)가 나타났다. 서양종과 동양종 꿀벌의 방화특성을 조사한 결과 꽃에 머무는 시간은 서양종 꿀벌 이 평균 6.9초, 동양종 꿀벌이 7.0초로 같은 수준이었으나(p>0.05), 꽃 간 이동시간은 동양종 꿀벌이 2.4초로 서양 종꿀벌(3.1초)보다 20% 유의미하게 짧은 것으로 나타났다(p=0.011). 따라서 같은 시간에 동양종 꿀벌이 서양종 꿀벌보다 더 많은 꽃을 방문 할 수 있을 것으로 생각된다.

The clearwing moth, Synanthedon bicingulata (Staudinger, 1887), is a pest that infests various species of cherry trees. However, genetic information regarding the genus Synanthedon including S. bicingulata, is limited. In this study, we sequenced a complete mitochondrial genome (mitogenome) of the species. The 16,255 bp of S. bicingulata mitogenome differs from the typical gene arrangement formed in Lepidoptera: trnQ-trnS2-trnM-trnI arrangement between the A+T-rich region and the ND2 junction. Moreover, the genome has untranslated repetitive sequences in the intergenic space between lrRNA and trnV, as well as the CGA start codon in COI and the TTG start codon in ATP8. Similar observations are noted in species belonging to the tribe Synanthedonini within the genus Synanthedon.

The two-spotted spider mite, Tetranychus urticae Koch, is one of the economically important agricultural pests globally, as it attacks a wide range of vegetable and horticultural crops. In this study, we evaluated spatial repellent and oviposition deterrent activities of T.urticae in response to fifteen compounds derived from ester-containing natural products. To evaluate the tests, we used bridge two-choice test and host two-choice test in laboratory conditions. Among the eight compounds showed spatial repellent and oviposition deterrent activities against T. urticae at the 20 mg dose and some compounds had the activities at lower dose. We also conducted two-choice test with a blend and single compounds to determine which showed stronger spatial repellent and oviposition deterrent activities. In host two-choice test, we evaluate repellence between distance of compounds. This study concluded that series compounds from ester-containing natural products have the potential to be used managing T. urticae in the field.