곤충호텔은 곤충이 서식할 수 있도록 인위적으로 만든 구조 물로서 정원이나 텃밭 등 다양한 곳에서 활용되고 있다. 하지만 식생유형, 재료, 설치 방식 등에 따른 곤충 유입 효과에 대한 검증은 부족하며, 국가 또는 지역에 따른 곤충의 종류와 생육환 경이 다름에도 불구하고 곤충호텔에 대한 국내 연구는 전무한 실정이다. 따라서 본 연구에서는 국립수목원을 대상으로 구체적 인 식생유형 및 재료에 따른 유입 곤충 특성을 확인하고, 국내 실정에 맞는 효과적인 곤충호텔 설치 및 관리방안에 대한 기준 을 제시하고자 하였다. 조사구는 세 가지 식생유형(초지, 침엽수 림, 활엽수림)으로 구분하였으며, 각 식생의 조사구에 두 가지 재료(참나무, 잣나무)를 활용한 곤충호텔을 설치하였다. 조사는 2년동안(2022~2023) 진행되었으며, 매년 4~9월까지 주 1회 씩(총 48회) 곤충호텔의 유입 곤충을 직접 채집하였다. 곤충호 텔에서 채집된 곤충은 총 9목 46과 129종 3,057개체로, 2022 년에는 7목 34과 85종 1,750개체, 2023년에는 8목 35과 77종 1,307개체가 출현하였다. 연도별로 비교하면 1차년도에 유입 된 곤충의 개체수가 2차년도 보다 약 1.3배 많았고, 재료의 부식 에 따른 곤충의 구성도 달라졌다. 식생유형에 따라 구분하면 기간에 상관없이 활엽수림에서 유입 곤충이 가장 많았고, 침엽 수림에서 가장 적었다. 또한 재료에 따른 구분에서는 참나무가 잣나무보다 유입 곤충이 많았다. 참나무의 경우 유입 곤충의 연도별 차이가 적은 반면, 잣나무는 1년차에 비해 2년차에 약 2.3배 줄었다. 상관분석과 계층적 군집분석을 통한 곤충의 유입 특성은 식생유형보다는 재료의 영향이 큰 것으로 확인되었고, 식생유형만 비교했을 경우 초지에 비해 활엽수림과 침엽수림의 유사성이 높았다. 결론적으로 생물 다양성 증진을 목적으로 곤 충호텔을 설치하고자 한다면 초지나 활엽수림에 참나무 재료를 사용하는 것이 유리하며, 잣나무 재료를 이용한다면 1년 주기로 재료 교체가 필요할 것으로 판단된다. 하지만 특정 재료를 선호 하는 곤충의 기주특이성이 확인되었기 때문에 다양한 종류의 재료를 같이 사용하는 것도 하나의 방법이라고 사료된다. 향후 이를 기반으로 전시원에서 실질적으로 활용할 수 있는 곤충호텔 모델 개발로 확대해 나갈 예정이다.

국립수목원 전시원의 지속가능하고 친환경적인 관리를 위하 여 유기물 멀칭재 처리에 대한 잡초 억제 효과를 조사하였다. 대표적 잡초인 쑥군락과 바랭이군락을 대상으로 고정방형구 (1×1㎡)를 3반복 설치하였다. 처리구의 유기물 멀칭재는 5가지 재료로 활엽수 우드칩, 침엽수 바크, 갈참나무 낙엽, 메타세쿼이 아 낙엽, 칠엽수 과피를 사용하였다. 멀칭재 중에서 갈참나무 낙엽, 활엽수 우드칩, 칠엽수 과피가 비교적 잡초 억제 효과가 높은 것으로 나타났다. 갈참나무 낙엽은 지중 온도를 낮게 유지 하는 효과가 상대적으로 좋았기 때문에 토양의 보습 효과가 뛰 어날 것으로 판단된다. 유기물 멀칭재 처리는 일년생식물의 발 생은 억제하고, 지중식물 및 반지중식물의 생육에 도움을 주는 효과가 있었다. 멀칭재의 무게보다 재료에 의한 빛 차단 면적이 잡초를 억제하는 주된 요인이었다. 본 연구 결과는 국립수목원 전시원에서 발생하는 부산물을 활용한 친환경적 관리 방안 수립 에 활용될 것이며, 향후 잡초의 발생 시기에 따른 멀칭 시기 및 유기물 멀칭재의 유지 관리에 대한 추가적인 연구가 필요하다.

Following the previous study, which investigated the pharmacological properties of the Technekitty injection (Tc-99m), the toxicity of a single intravenous administration of the Technekitty injection (Tc-99m) and the side effects that may occur at the diagnostic dose were confirmed. The Technekitty injection (Tc-99m) was administered intravenously once at a dose of 0, 0.67, 2.0, and 6.0 mCi/kg to 5 male and female rats per group. Mortality, general symptom observation, and weight measurement were performed for 2 weeks, followed by observation of autopsy findings. There were no deaths, and no statistically significant weight change was observed. No abnormal systemic signs related to the Technekitty injection (Tc-99m) were observed. These results confirmed that Technekitty injection (Tc-99m) can be safely administered intravenously at doses up to 6.0 mCi/kg. Additionally, technetium-99m at an average dose of 2 mCi (74 MBq) has been verified as a diagnostic dose without adverse effects, allowing the Technekitty injection (Tc-99m) to be used safely without side effects at this dosage. This study demonstrates that the Technekitty injection (Tc-99m) has a wide safety margin, supporting its potential for clinical application. Moreover, these findings align with the nonclinical safety standards for radiopharmaceuticals, reinforcing its utility in veterinary medicine. The Technekitty injection (Tc-99m) is expected to be applicable for clinical diagnosis as a veterinary drug in Korea.

본 연구는 국립수목원 내 전문전시원에 발생한 이입식물을 대상으로 2015년부터 2023년까지 이입식물 분포조사를 시행 하였다. 그 결과 자생식물 299종, 귀화식물 49종, 재배식물 13 종 총 63과 214속 361종이 조사되었다. 국화과가 63종으로 가장 많았고, 벼과, 사초과 순으로 발생하였다. 이입식물의 이입 경로를 분석하기 위하여 광릉숲에 분포하는 식물상과 비교 분석 한 결과 309종이 광릉숲에 분포하는 식물로 확인되었으며, 전 문전시원 내 식재 식물이 확산된 경우가 17종으로 조사되었다. 조사된 귀화식물 49종을 원산지별로 구분하면 유럽과 북아메리카가 69.4%, 이입 시기별 1기와 3기의 비율이 90%를 차지하였 다. 귀화식물의 귀화도 분포 등급을 적용한 결과 4등급 이상이 면서 이입 시기가 3기인 식물 9종은 빠르게 확산할 우려가 큰 식물이므로 지속적인 모니터링이 필요하다. 국립수목원에 어느 정도 귀화식물이 분포하는지를 가늠하는 도시화지수를 산출한 결과 15.0%로 나타났으며, 환경부 지정 생태계교란 야생식물 중 돼지풀, 단풍잎돼지풀, 애기수영, 미국쑥부쟁이가 출현하였 다. 이입식물은 자생식물군락과 전문전시원 내 관람로에 침입하 여 세력권을 확장하고 우점하는 경향이 있어 각각의 생태적 특 성을 고려한 제거 방법이 필요하다.

This study presents a rapid and sequential radiochemical separation method for Pu and Am isotopes in radioactive waste samples from the nuclear power plant with anion exchange resin and TRU resin. After radionuclides were leached from the radioactive waste samples with concentrated HCl and HNO3, the sample was allowed to evaporate to dryness after filtering the leaching solution with 0.45 micron filter. The Pu isotopes were separated in HNO3 medium with anion exchange resin. For leaching solution passed through anion exchange column, the Am isotopes were separated with TRU resin. The purified Pu and Am isotopes were measured by alpha spectrometer, respectively, after micro-precipitation of neodymium. The sequential radiochemical separation of Pu and Am isotopes in radioactive waste samples using anion exchange resin and TRU resin was validated with ICP-MS system.

Entomopathogenic fungi are used to produce raw materials by applying solid culture technology using grains. But there are various problems such as low production efficiency and cross-contamination. Solvum Co., Ltd. conducted research on liquid culture technology to develop a method that can overcome these shortcomings of solid culture technology. We conducted research and development on using Beauveria bassiana 331R to observe the culture according to the seed inoculation amount in a 30 L fermenter, it was carried out at 1.0 % (v/v) and 10.0 % (v/v). Although there was a difference of 1 day, 1.0 %(v/v) seed inoculation was observed to be more than twice that, and active blastospores and yield were observed at over 95.0 %. As a results, it was determined that cost and efficient production would be possible during the culture process in mass production. Based on these experiments, a 300 L fermenter was cultured with 1.0 % (v/v) seed inoculation, resulting in a yield of 1.24E+09 CFU/mL on the 6th day of cultivation. As a result of freeze-drying using the final culture medium, it was confirmed that the production yield was improved by 113.0 % compared to the control.

This study was conducted to calculate the damage of Italian ryegrass (IRG) by abnormal climate using machine learning and present the damage through the map. The IRG data collected 1,384. The climate data was collected from the Korea Meteorological Administration Meteorological data open portal.The machine learning model called xDeepFM was used to detect IRG damage. The damage was calculated using climate data from the Automated Synoptic Observing System (95 sites) by machine learning. The calculation of damage was the difference between the Dry matter yield (DMY)normal and DMYabnormal. The normal climate was set as the 40-year of climate data according to the year of IRG data (1986~2020). The level of abnormal climate was set as a multiple of the standard deviation applying the World Meteorological Organization (WMO) standard. The DMYnormal was ranged from 5,678 to 15,188 kg/ha. The damage of IRG differed according to region and level of abnormal climate with abnormal temperature, precipitation, and wind speed from -1,380 to 1,176, -3 to 2,465, and -830 to 962 kg/ha, respectively. The maximum damage was 1,176 kg/ha when the abnormal temperature was -2 level (+1.04℃), 2,465 kg/ha when the abnormal precipitation was all level and 962 kg/ha when the abnormal wind speed was -2 level (+1.60 ㎧). The damage calculated through the WMO method was presented as an map using QGIS. There was some blank area because there was no climate data. In order to calculate the damage of blank area, it would be possible to use the automatic weather system (AWS), which provides data from more sites than the automated synoptic observing system (ASOS).

The GMT-Consortium Large Earth Finder (G-CLEF) is the first instrument for the Giant Magellan Telescope (GMT). G-CLEF is a fiber feed, optical band echelle spectrograph that is capable of extremely precise radial velocity measurement. G-CLEF Flexure Control Camera (FCC) is included as a part in G-CLEF Front End Assembly (GCFEA), which monitors the field images focused on a fiber mirror to control the flexure and the focus errors within GCFEA. FCC consists of an optical bench on which five optical components are installed. The order of the optical train is: a collimator, neutral density filters, a focus analyzer, a reimager and a detector (Andor iKon-L 936 CCD camera). The collimator consists of a triplet lens and receives the beam reflected by a fiber mirror. The neutral density filters make it possible a broad range star brightness as a target or a guide. The focus analyzer is used to measure a focus offset. The reimager focuses the beam from the collimator onto the CCD detector focal plane. The detector module includes a linear translator and a field de-rotator. We performed thermoelastic stress analysis for lenses and their mounts to confirm the physical safety of the lens materials. We also conducted the global structure analysis for various gravitational orientations to verify the image stability requirement during the operation of the telescope and the instrument. In this article, we present the opto-mechanical detailed design of G-CLEF FCC and describe the consequence of the numerical finite element analyses for the design.

Following the previous study, the toxicity of a single subcutaneous administration of the Thyrokitty injection (I-131) and the side effects that may occur at therapeutic doses were confirmed. The Thyrokitty injection (I-131) was administered subcutaneously once at a dose of 0, 2.0, 6.0, and 18.0 mCi/kg, 5 male and female rats per group, and mortality, general symptom observation, and weight measurement were performed for 2 weeks, followed by observation of autopsy findings. There were no deaths, and no statistically significant weight change was observed. Mild hair loss, fissures, and crusting were observed by general symptom observation, but it was not a toxic change related to the Thyrokitty injection (I-131). Gastric atrophy and a decrease in the size of the spleen were observed by the autopsy. As a results of single subcutaneous administration of the Thyrokitty Injection (I-131) to rats at a maximum dose of 18.0 mCi/kg, a decrease in the size of the spleen and gastric atrophy were observed as the dose of the Thyrokitty Injection (I-131) increased, which may be related to the test substance. No abnormal findings related to the Thyrokitty injection (I-131) were observed. Therefore, the approximate lethal dose of the Thyrokitty injection (I-131) was 18.0 mCi/kg or more. In addition, as reported for the treatment of feline hyperthyroidism with radioiodine (131I), side effects of the Thyrokitty injection (I-131) are expected to be extremely rare. Temporary dysphagia and fever may occur, but it will recover naturally. It should be administered with caution in cats with diseases such as urinary system, cardiovascular system, gastrointestinal system and endocrine system, especially with kidney disease. And it should not be used in cats who are pregnant, lactating, or likely. It is expected that the Thyrokitty injection (I-131) can be used for clinical treatment in Korea as a veterinary drug.

Radioiodine (131I) has been used for the treatment of feline hyperthyroidism since the 1990s in the USA and Europe, and it is recommended as the most effective treatment for feline hyperthyroidism because it has a high therapeutic effect, small side effects, and does not require anesthesia. In this study, the pharmacological properties of the Thyrokitty injection (I-131), which is being developed as a treatment for feline hyperthyroidism, using radioiodine (131I) as an active ingredient, was tested. The %cell uptake of the Thyrokitty injection (I-131) in FRTL- 5 thyroid cells was 0.410 ± 0.016%, which was about 18 times higher compared to Clone 9 hepatocytes, and it was decreased by 30.7% due to the competitive reaction with iodine (sodium iodide). In addition, the %cell growth of the FRTL-5 thyroid cells was reduced by 25.0% by treatment with the Thyrokitty injection (I-131). As a result of the tissue distribution test, the Thyrokitty injection (I-131) was distributed at the highest concentration at 0.083 hours (5 minutes) after subcutaneous administration to animals in most organs except the stomach, small intestine, large intestine, muscle and thyroid gland, and it was excreted mainly through the kidneys. The stomach and thyroid gland showed a typical distribution pattern observed when radioiodine (131I) was administered. In addition, about 78.45% of the total amount of excretion was excreted within 48 hours, of which more than 85% was excreted in urine. In conclusion, the Thyrokitty injection (I-131) has the same mechanism of action, potency, absorption, distribution, metabolism and excretion characteristics as radioiodine (131I) reported in connection with the treatment of feline hyperthyroidism. In the future, using the results of this study, it is expected that the Thyrokitty (I-131) could be safely used in the clinical treatment of feline hyperthyroidism.

Honey bees are affected by a variety of factors, so they have to be thoroughly managed according to their lifestyle. The activity of the honey bee foragers represent an important parameter of the hive state. Here, the real-time and automatic monitoring system using dual infrared sensors was applied for counting the foraging activity of honey bees based on ICT. According to this study, this system is very accurate with a relative error of 3.98% / 4.43% compared to manual counting through video analysis. This system showed the scalability of the system through the internal and external temperature sensors connected through the main board and BLE module. Furthermore, the data measured through this system for one month were analyzed, the monthly average foraging activity and the number of lost foragers were measured (1.88% of outgoing bees), and at the same time, the foraging patterns according to the changes of temperature and time were analyzed. This study suggests that the development of apicultural, scientific and educational materials with more powerful real-time monitoring tools through expansion of a complex monitoring system and big data accumulation.

The spotted wing drosophila(Drosophila suzukii)is one of the serious fruit pest that has a wide range of fruit crops like blueberries, cherries and grapes. It came up the larvae cause dagmage by feeding on the pulp inside fruits and berries and spotted wing drosophila also has a adversely affect export to Austrailia and New zealand as a quarantine pest. This study conduct to survey occurrence of drosophila spp. from 2016 to 2018 on harvest period of grapevine in vinyard of Okcheon ,Yeongdong and fruit packing place for export to New zealand and USA. Vinegar and wine mixture was used to attract fruits flies. We also investigated to what color is the best stimulus to catch a vinegar flies. Our result shows that significant differences were observed in total flies trapped in red and black colors. This study will contribute to future research for monitoring and control drosophilid flies.