Lepidopteran pests monitoring in adult stage was generally performed using delta or corn typed trap including rubber septa impregnated sex pheromone (lure). Sometimes, unfortunately trapped samples were severly damaged because of biotic and/or abiotic environments such as micro-organism, predator and rain, sticky material, respectively. In our case, we monitored potato tuber moth, PTM, Phthorimaea operculella distribution during 2009~2012 in Korea. However, we encountered unexpected problem, another species can be trapped in species specific sex pheromone trap. Therefore, species confirmation was needed in trapped samples. Here we developed confirmation method by direct PCR (without DNA extraction) or sequencing methods which trapped samples that cannot identified by morphologically. We designed multi-plex PCR universal primers and species specific primers in rRNA region because to check the success of PCR and species identification. This direct PCR method can be applied in other species confirmation which monitored using pheromone trap.

The current investigation was undertaken to evaluate the antioxidant activities, tyrosinase inhibitory effects on the fruiting bodies of P. ostreatus extracted with acetone, methanol and hot water. The antioxidant activities were performed on β-carotene-linoleic acid, reducing power, 1,1-diphenyl-2-picrylhydrazyl free radical scavenging, and ferrous chelating abilities. In addition to this, phenolic acid and flavonoids contents were also analyzed. Methanolic extract of P. ostreatus showed the strongest β-carotene-linoleic acid inhibition as compare to others exracts. At 8 mg/ml, acetonic extract showed a high reducing power of 1.54. The scavenging effects on 1,1-diphenyl-2-picrylhydrazyl radicals, acetonic extract was effective than other extracts. The strongest chelating effect (85.66%) was obtained from the acetonic extract at 1.0 mg/ml concentration. Antioxidant activities of the extracts from the fruiting bodies of P. ostreatus were increased with the increasing concentration. After application of reverse phase high performance liquid chromatography, coupled to a diode array detector and electrospray ionisation mass spectra, six phenolic compounds namely, gallic acid, protocatechuic acid, naringenin, hesperetin, formononetin and biochanin were identified from acetonic extract. Tyrosinase inhibition of acetonic, methanolic, and hot water extracts of P. ostreatus were increased with the increasing of concentration. Results revealed that methanolic extract showed good, while acetonic and hot water extracts showed moderate activities of the tyrosinase inhibition at the concentration tested. This study suggests that fruiting bodies of P. ostreatus can potentially be used as a readily accessible source of natural antioxidants.

본 연구에서는 준 회분식 교반조를 사용하여 polybutene (PB)와 polyisobutylene (PIB)고분자를 용해한 벤젠 용액을 연속상, 물을 불연속상으로 구성한 w/o 에멀션액막에 CO2을 흡수시켜 흡수속도를 측정하였다. 점탄성을 나타내는 Deborah 수를 사용하여 점탄성 비뉴튼액체에서 구한 부피물질전달계수 (kLa)를 고찰하고, 수용액에 첨가한 2-amino-2-methyl-1-propanol(AMP)와 CO2의 반응 메카니즘을 해석하였다.

Background : The purpose of this study was to big strong and root in the disease, many high quality seed of Lithospermum erythrorhizon selection by fostering, expanding the spread on a farm and Hongju subsistence production base and raw materials medicines of sources deployment for process is to contribute to increasing farm income. Methods and Results : Collected variety into the chemical mutation breeding method, Jecheon of Chungbuk, was conducted in 2001. In 2002 and 2003, two～ three individual selection in 2005 as it produces the 24 individual in 2004. From 2006 to 2010, pure line isolation as it cultivated and one foundation group. Promising in 2011, select one system. Replicated yield trial for two years beginning in 2012 a result of the stem length, providing high -quality “No. 1, Jeollanam-do” majority by assigning system name. Naju, Jangheung-gun, local excellence by implementing a local adaptability test recognized in two years. To breed a new variety named as a dwelling “ Daehong” of Lithospermum erythrorhizon the review by fostering december 2014 to be registered. Conclusions : Leaf type is the broad lancet, flowering time is new variety six days than Jecheon native variety fasted. New variety of stem length and stem thickness are check variety (87cm, 8.25mm) of 9cm tall and 0.6mm thick. Seed yields has native variety as much as 81 % compared to the many into 45.6 kg/10a. The shikonin contents of roots was 1.3 times higher compared to check variety (447 ㎍ / g). The dried root yields of the new variety are 221kg/10a a many native variety than 50%.

‘Zasoojeongchal’ is a new colored naked barley cultivar developed from the cross between HB83072〔Tapgolbori / HB 78100-B-17-3-2〕, a disease resistant and good quality line and ‘Dokusima mochi hadaka’, colored naked cultivar with waxy endosperm by the Honam Agricultural Research Institute (HARI), NICS, RDA in 2006. An elite line CNB7-13-37-2-5-3, was selected in 2002 and designated as ‘Iksan 81’. It showed good agronomic performance in the regional yield trials (RYT) from 2005 to 2006 and was released with the name of ‘Zasoojeongchal’ possessing purple colored waxy endosperm. The average heading and maturing dates of ‘Zasoojeongchal’ were May 2 and Jun. 7, which were two and five days later than those of the check cultivar ‘Saechalssalbori’ respectively. The new cultivar, ‘Zasoojeongchal’, had 75cm of culm length that was 4cm shorter than that of ‘Saechalssalbori’, and 5.4 cm of spike length. It showed 607 spikes per m2, 63 grains per spike, 27.2 g of 1,000-grain weight, and 771 g of test weight. ‘Zasoojeongchal’ showed weaker winter hardiness, but better resistance to powdery mildew and BaYMV (Barley yellow mosaic virus) than those of the check cultivar, ‘Saechalssalbori’. It showed higher ß-glucan content, anthocyanin content, water absorption rate and expansion rate than those of the check cultivar, ‘Saechalssalbori’. Its average yield of the pearled grain in the regional yield trial was 2.67 MT/ha in upland, and 3.49 MT/ha in paddy field, which were 26% and 6% lower than those of the check cultivar, respectively. This cultivar would be suitable for the area above the daily minimum temperature of -6℃ in January in Korean peninsula.

‘Yuyeon’, a new ruminant-palatable barley cultivar, was developed from the cross between ‘Suwon311’ and SB86648〔CMB81A-2149 / Saeolbori // Buheung / Gangbori / 5 / Bera-olbori / Horisee-Buheung // bengei / 3 / Olbori-Daechi-Hagane / 4 / T.beared〕by the Honam Agricultural Research Institute (HARI), NICS, RDA in 2006. Among the cross made in 1996, a promising line, SB961012-B-B-1, showed good characteristics in potential forage yield in the yield trial tested in Suwon in 2001. In 2004, it was designated as ‘Suwon 406’ and placed in regional yield trials at five locations around Korea for three years from 2004 to 2006, and was released as the name of ‘Yuyeon’. It belong to maturity group Ⅲ and shows erect leaf type and compact spike with types of hoods. Its heading date was Apr. 27, which was 2 days earlier than that of the check cultivar ‘Olbori’ (‘Sunwoo’), and early dough stage, which was May. 30 which was same date of that of the check cultivar. ‘Yuyeon’ showed weaker winter hardiness, but better resistance to lodging, shattering and BaYMV than those of check cultivar. It showed higher crude protein content, grade of silage quality than those of check cultivar. The average forage yield in the regional yield trial was 10.47 MT/ha, which was 1% lower than that of the check cultivar. Cattles fed with silage from ‘Yuyeon’ (hood type) showed higher feed concentrate requirement (12.9 kg/day/body) and daily weight gain (1.46 kg/day) than those of cattles fed with silage from ‘Olbori’ (rough awn). This cultivar would be suitable for the area whose daily minimum temperature was above -8℃ in January in Korean peninsula.

A ruminant-palatable barley cultivar, Soman (Hordeum vulgare L.), with early maturing, high silage quality and high yield, was developed from the cross between SB79124〔Bengei-Ginomeo-Buheung-Morsen-Hagane/Gangbori〕and SB77189 〔Samjug/Riso82//Olbori〕in 1994. A promising line “SB941067-B-B-B-45” was selected in 1998. It performed well in the preliminary and advanced yield trials for two years from 2002 to 2003. The line was designated ‘Suwon 404’ through the regional yield trials (RYT) at six sites for three years from 2004 to 2006. Its growth habit was winter type (Ⅲ) with greenish leaves, medium culm and compact density of spike setting. Its heading date and early dough stage were Apr. 23 and May 26 respectively, which were 6 days earlier than those of check cultivar ‘Olbori’. ‘Soman’ showed weaker winter hardiness, but better resistance to lodging, shattering, and BaYMV (Barley yellow mosaic virus) than those of check cultivar ‘Sunwoo’. It showed 1.7% higher TDN content on whole-crop-silage than that of check cultivar ‘Sunwoo’. Its average of forage yield in regional yield trials was 10.31 MT/ha, which was 4% higher than that of the check cultivar.

The six rowed and naked barley cultivar, “Cheongho” (Hordeum vulgare L.), showing earliness, good cooking quality, resistance to powdery mildew and barley yellow mosaic virus disease (BaYMV), winter hardiness, lodging tolerance, and high yield in paddy fi

A six-rowed naked barley cultivar, Donghanchal (Hordeum vulgare L.), with low-amylose, winter hardiness, viral disease resistance and good quality, was developed from the cross between the advanced line “Masangwamac/Kangbori*7” and Naehanssalbori in 1993.