본 논문에서는 유통 농산물 잔류농약 부적합 현황 분석을 위하여 네트워크 분석 기법을 적용하였다. 분석용 데이터는 『2017년도 식품안전관리지침』내 “유통농산물별 잔류농약 부적합 현황”을 참고하였으며, 주요 분석 기법 으로는 중심성 분석(연결정도 중심성, 근접 중심성, 매개 중심성)을 적용하였다. 연결정도 중심성 분석결과 chlorpyrifos와 diazinon에서 “연결된 노드”가 가장 많은 것으로 확인되었다. 근접 중심성에서는 19종을 제외한 농약성분들 사이에서 유사한 정도를 보여주고 있었다. 매개 중심 성 분석 결과에서는 fluioxonil과 chlorpyrifos가 가장 높은 경향을 보여주어 이들이 농약성분 네트워크에서 “가교” 역 할을 수행 하는 것으로 파악되었다. 네트워크 분석 결과, 기존의 통계분석을 통해서는 분석 할 수 없었던 농약성분 들 사이의 “관계” 데이터를 확인 할 수 있었다. 향후 이러한 분석기법은 최적화/정교화 과정을 통해서 보다 정밀하게 농약성분 부적합 현황 분석을 위한 도구로서 적용되리라 기대한다.
본 연구에서는 조명나방 성충의 우화시간대, 나이에 따른 암컷의 유인해동양상과 교미능역, 1일부터 5일된 암컷이 분비한 성페로몬에 대한 3일된 수컷의 반응 그리고 성페로몬샘의 구조와 위치를 조사하였다. 광주기를 16L/8d로 햇을 때 성충운 일잔벅으로 암시간대 시작 한시간 이전부터 1~2시간이후까지 우화 하였다. 그리고 2일과 3일된 암컷ㅇ에서 6시부터 7시 사이에 가장 황성한 유인행동을 보였으며, 3일된 암컷에 대한 수컷의 반응이 암시간대 5~8 시간에서 가장 높게 나타났다. 교미쌍은 암시간대 4~7시간에서 가장 많이 관찰되었다. 1일부터 4일된 암컷중 성페로몬샘을 돌출시킨 개체들은 65%이상의 교미율을 보여주었다. 그리고, 성페로몬샘을 돌출한 2~3일된 암컷은 1~5일된 각각의 수컷과 60%이상의 교미율을 보여주었다. 암컷의 성페로몬샘으로 추측되는 두꺼운 세포층들은 복부 8번째, 9번째, 그리고 9번째와 10번째 사이의 막질성 표피층에 위치하고 있었다.
미국흰불나방 휴면번데기에 5 /g(생체중) 이상의 탈피호르몬을 처리하면 처리된 용의 약 50%가 우화했으며, 10 이나 12.5 /g의 처리구에서는 몇마리의 기형적인 성충이 나타났다. 한편 탈피호르몬의 체내함량을 적정한 결과 정상용은 용화 후 6일째에 약 3.2 /g의 최대값을 보였으며, 탈피호르몬을 처리한 용에서는 처리 6일째에 4.1 /g의 최대값을 보였다. 그러나, 휴면 용에서는 낮은 탈피호르몬 함량을 보였는데 측정 기간중 얻은 최대치는 1.73 /g 정도였다. 탈피호르몬을 처리한 휴면 용의 글리코겐함량은 정상용 보다 낮았으나, 성충조직 발육기간 동안의 발육양상은 정상 용의 경우와 비슷하였다. 또한 같은 처리를 받은 휴면 용의 포도당-솔비톨과 트레할로스의 농도는 처리 후 6일째에 최대값을 보였는데, 그 수준은 정상적으로 발육하는 개체의 경우보다 높았다. 탈피호르몬 처리를 받은 휴면용의 혈림프 내 수용성단백질 함량은 정상 용의 그것보다 낮았으며, 혈림프를 제외한 전체 조직 내에서 정상용의 경우보다 높았다.
The influence of triploidization on histological characteristics of retina, trunk kidney, liver and midgut tissue, and cell cycle of tail fin and gill tissue in far eastern catfish, Silurus asotus were analyzed. In the infertile triploid fish, the nucleus and/or cell size of secondary proximal tubule cells of trunk kidney, hepatocyte and midgut epithelium are much larger than those of the corresponding cells in the diploid fish (P<0.05). However, triploid tissue showed fewer number of outer nuclear layer in retina and nuclei in secondary proximal tubule of trunk kidney than those for diploid tissue. The mean percentages of the Gl-, the S- and the G2+M-phase fractions were 92.5%, 3.2% and 4.3% in tail fin tissue of diploid, and 93.4%, 2.6% and 4.0% in those of triploid, respectively. There were no significant differences in the percentages of each cell cycle fraction between diploid and triploid. The mean percentages of each phase fractions were 75.1%, 11.1% and 13.8% in gill tissue of diploid and 85.2%, 8.9% and 5.9% in those of triploid, respectively. The differences of cell cycle between tail fin tissue and gill tissue were statistically significant in diploid and triploid (P<0.05). Also, the differences between diploid and triploid were statistically significant in tail fin tissue and gill tissue (P<0.05). Cyclin D1 and cyclin E expressions were not significantly difference between gill tissue and tail fin tissue, and protein expressions of induced triploid were higher than those of diploid. Results from this study suggest that some characteristics in the triploid exhibiting larger cell and nucleus size with fewer number of cell than diploid can be used as an indicator in the identification of triploidization and ploidy level in far eastern catfish.
The influence of triploidization on histological characteristics of retina, trunk kidney, liver and midgut tissue, and cell cycle of tail fin and gill tissue in far eastern catfish, Silurus asotus were analyzed. In the infertile triploid fish, the nucleus and/or cell size of secondary proximal tubule cells of trunk kidney, hepatocyte and midgut epithelium are much larger than those of the corresponding cells in the diploid fish (P<0.05). However, triploid tissue showed fewer number of outer nuclear layer in retina and nuclei in secondary proximal tubule of trunk kidney than those for diploid tissue. The mean percentages of the Gl-, the S- and the G2+M-phase fractions were 92.5%, 3.2% and 4.3% in tail fin tissue of diploid, and 93.4%, 2.6% and 4.0% in those of triploid, respectively. There were no significant differences in the percentages of each cell cycle fraction between diploid and triploid. The mean percentages of each phase fractions were 75.1%, 11.1% and 13.8% in gill tissue of diploid and 85.2%, 8.9% and 5.9% in those of triploid, respectively. The differences of cell cycle between tail fin tissue and gill tissue were statistically significant in diploid and triploid (P<0.05). Also, the differences between diploid and triploid were statistically significant in tail fin tissue and gill tissue (P<0.05). Cyclin D1 and cyclin E expressions were not significantly difference between gill tissue and tail fin tissue, and protein expressions of induced triploid were higher than those of diploid. Results from this study suggest that some characteristics in the triploid exhibiting larger cell and nucleus size with fewer number of cell than diploid can be used as an indicator in the identification of triploidization and ploidy level in far eastern catfish.
This study was designed to identify citizens’ awareness and satisfaction of Green Roof Projects (GRPs) in Changwon City, South Korea. Changwon City implemented a “Green Roof Promotion Planning” project according to the city’s codes and regulations from 2009 to 2011. The project introduced guidelines for ecological area rates and provided financial subsidies for the green roofing of private buildings. A citizens’ survey shows that 64.8 percent of respondents have a positive awareness of GRPs and think the projects’ priority should be on public buildings and commercial/office buildings. About 43.1 percent of green roof users were satisfied with green roofing in terms of housing improvement and energy saving. However, 34.6 percent of green roof users were not satisfied with regard to plant management. Educational level made a statistically meaningful difference in terms of the awareness of the project’s needs and participatory spirit. Statistics of the survey showed that citizens’ attitude of participation spirit was influenced by the increase in value of a building, green space expansion, and ecosystem conservation. However, their satisfaction was determined by housing improvements. The results of this survey call for a deliberate policy effort on the part of Changwon City to increase the projects’awareness, satisfaction, and participation
Sexual dimorphism is the most conspicuous difference between the sexes. This study examines possible sexual dimorphism and the relative growth patterns of morphometric characteristics in the marine medaka, Oryzias dancena for their potential to help differentiate between males and females of this species. The von Bertalanffy growth parameters estimated by a non-linear regression method were L∞=30.2 mm, K=3.22/year, and τ0=-0.05. All 18 characteristics measured showed a difference between males and females from 70 days after hatching. Each of these characteristics were significantly different between sexes (ANCOVA, P<0.05), and the ratio of standard length between sexes showed that males were larger than females for all five morphometric measurements. Fin length measurements were taken for 21 distances of anal fin and 7 distances of dorsal fin between landmarks. There were all differences for all dorsal fin rays between the males and the females and there is significant difference in 70 days after their hatch when the sexual dimorphism is presented. The significant difference (P<0.05) in fin ray for male and female was more greatly seen as they grow. Male marine medaka showed more rapid growth than females, with longer length, dorsal fins and anal fins. Differences in these characteristics will be useful during experiments when it is necessary to differentiate between sexes of marine medaka.
The three natural zeolites collected in Yungil-gun, Kyungsangbuk-do, Korea, were analyzed by means of chemical wet methods and X-ray diffraction. The results indicated that the primary species of those zeolites were clinoptilolite mixed with heulandite, feldspar, montmorillonite, and quartz. These zeolites were chemically treated with NaOH, Ca(OH)_2, and HCl solution and their differences were also studied with X-ray diffraction method. The capabilities of removing Cs^+ and Sr^2+ ions with chemically untreated zeolites, chemically treated zeolites, and also with synthetic zeolites were compared. The effect of other cations in removing Sr^2+ ions was also studied. The experimental results showed that Cs+ and Sr^2+ ions could be removed up to 98% and 95% respectively out of 5 ppm with chemically untrearted natural zeolites. The treatment of 0.02N-Ca(OH)_2 and that of 2N-NaOH were most effective in removing Cs^+ and Sr^2+ ions, respectively. It was found that the mountaintop of Sangjung 1-dong natural zeolite treated with 2N-NaOH was most efficient in removing Sr^2+ ions mixed with other cations, compared with any other chemically treated and untreated natural zeolites in this work.