The consumption of pre-treated vegetables (including fresh-cut vegetables) has been significantly increased because of their ease of use for cooking. Vegetable cutting machine has been widely utilized for producing fresh-cut vegetables or agricultural products with different sizes; however, its design standard is not specifically established depending on types of agricultural products. Therefore, this study was conducted to determine mechanical properties (compressive and shear force) of targeted agricultural products (radish, carrot, squash, cucumber, shiitake mushroom and sweet potato) for developing multipurpose vegetable cutting machine. According to ASAE standard (s368.3), compressive and shear force of targeted agricultural products were measured by using custom built UTM (universal testing machine). Shape type of samples and speed ranges (5~15 mm/min) of loading rate on bioyield and shear points were varied by targeted agricultural products. The range of averaged bioyield points of targeted agricultural products were between 7.89 and 146.98 N. On the other hands, their averaged shear points were from 22.50 to 53.47 N. Results clearly showed that the bioyield and shear points of targeted agricultural products were thoroughly affected by their components. As accumulating compressive and shear points of agricultural products, it can be technical feasible to establish the design standard and control mechanism of multipurpose vegetable cutting machine.

This study was conducted to investigate the quality characteristics and antioxidant properties of cookies supplemented with 1-4% (w/w) persimmon leaf powder (PLP). pH and density of cookie dough increased significantly but moisture content decreased significantly with increasing levels of PLP (p<0.05). The spread ratio and loss rate of cookies decreased significantly with increasing levels of PLP (p<0.05). Lightness (L*), redness (a*), and yellowness (b*) decreased significantly with higher amount of PLP (p<0.05). The use of PLP significantly increased the hardness of cookies while 2,2-diphenyl- 1-picrylhydrazyl (DPPH) and 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical scavenging activities were significantly elevated (p<0.05). The consumer acceptance test indicated that the addition of PLP up to 2% had a favorable effect on the consumer preferences in most attributes. Based on the overall observations, cookies with 2% PLP can take advantage of the functional properties of PLP without sacrificing consumer acceptability.

Both Nilaparvata lugens Stål and Laodelphax striatellus Fallén has been known as the major rice pests which were irregularly migrated into Korea by wind current from China. We re-arranged and compared the resistance profiles to detect the existence convergent adaptation by using the previously published dataset (N. lugens at 2014, 17:711-716 and L. Striatellus at 2016, 19:247-252 in J. Asia Pac. Entomol., respectively). Both species commonly exhibited the high level of resistance to imidacloprids. Especially, the immigrant populations of L. Striatellus exhibited higher resistance level to indigenous populations, suggesting that they would settle down in Korea with insecticide resistance traits being predisposed. To detect the convergent adaptation to insecticide, pearson correlation analysis were employed by comparing mean dosage of LD50 and resistance ratio to nine insecticides. Strikingly, high and similar dosage responses were observed between the two species with high correlation coefficients of 0.928 (df=7, P <0.001) and 0.950 (df=7, P <0.001), respectively, in the comparison of LD50 vs. resistance ratio. This finding indicates that convergent adaption has occurred in both species through consistent selection by insecticides with similar usage patterns.

Entomopathogenic fungus is a useful control agent to sucking type insect such as whitefly and aphid. The fungi are influenced by some environmental factors such as relative humidity, temperature and UV and cause slow and fluctuation in pest control efficacy. Especially, UV kills conidia or spores of entomopathogenic fungi and a mycopesticide using fungi has short control period in field. UV intensity changes from season to season. Survival rate of entomopathognic fungi treated may differ from seasons and will show different control efficacy. Therefore, we conducted a study to estimate the persistence of an Isaria javanica isolate, which was already reported as sweet potato whitefly control agent, in potted greenhouse soil planted different crops. The number of survival spore decreased gradually and differ from seasons.

Insect pests have been a serious problem over many years and remain a major threat for food production. Although chemical pesticides are major pest control strategies, use of microorganisms such as entomopathogenic bacteria, fungi, viruses and nematodes have continuously increase last few decades to minimize the use of agrichemicals. According to BBC research, the global biocontrol market was about $2.1 billion in 2011, and this is expected to rise $3 ~ 4 billion by 2017. Over 50 entomopathogens are commercially produced and used augmentatively as microbial pesticides. About 175 biopesticide active ingredients and 700 products have been registered worldwide. Bacillus thuringiensis (Bt), Beauveria bassiana, Metarhizium spp., nuclear polyhedrosis virus and Steinernema spp. are the most popular control agents used in plant protection. Among the microbial control agents Bt products have more than 50% of market share. In Korea, only 13 environmentally-friendly crop protectants were registered for insect pest control in 2015. Market share is very low and has grown slowly. We will discuss how we can expand the market with our techniques.

The aim of this study is to improve satisfaction with Namhae coastal landscape by analysing the image and satisfaction level that visitors have of Daraengyi village. The result of study is summarized as follows. According to the analysis of image and satisfaction, ‘harmony with nature and ecology (sea and mountain)’, and ‘cleanliness of travel destination’ were evaluated highly while ‘variety of several events’, and ‘appropriacy of education program’ got a low evaluation and ‘the satisfaction level’ was found to be high. The image and satisfaction with coastal landscape variables were; experience factor; familiarity factor; variety factor; scenery factor; service factor etc. The image and satisfaction factor of the target area was found to affect overall satisfaction. It was also found that overall satisfaction differed depending on use pattern (the reason of visit, the length of one’s visit and the repeat visit intention). To improve satisfaction with coastal landscape and induce revitalization, Daraengyi village should solve the problems highlighted by the five factors, and strive for the image improvement and management development.

본 연구는 남해 다랭이마을의 해안경관 이용자의 이용행태를 분석하여 해안경관 활성화 및 관리방안 제고를 위한 기초자료를 제공하고자 한다. 연구의 결과를 요약하면 다음과 같다. 일반속성 분석결과, 다랭이마을 이용객은 남성보다 여성이 많았으며, 연령은 20대~40대로써 대부분 대학졸업 이상이 었다. 거주 지역별 나이에 따른 교차분석 결과, 시간과 경제적인 여유가 있는 이용객이 많은 것으로 보인다. 직업은 회사 원이 높게 나타났고 기혼자가 많았다. 이용행태 분석결과, 다랭이마을 인지정도에 있어서는 잘 모른다고 하였고, 방문목적은 보물섬 남해관광이 가장 많았으며, 방문이유는 해안의 자연경치 및 경관의 아름다움이 가장 높게 나타났다. 동행형태는 친구 또는 가족이 많았다. 다랭이마을에 도달하는 시간은 2~3시간 이내이며, 관람에 소요되는 시간은 대부분 2시간 이내로 분석되었다. 이는 친구 또는 가족, 연인의 교류장소로 방문하며 대상지를 관람하는데 많은 시간을 할애하지 않는 것으로 보인다. 교통 이용수단 은 자가용이며, 대부분 첫 방문이 많았고, 이용자의 87.5%가 재방문을 희망하고 있었다. 다랭이마을의 이용행태에서 나타난 체류기간, 재방문의사 등은 해안경관의 전반적인 만족도와 통계적인 차이를 보였으며 특히 재방문을 희망하는 이용객은 전체만족도와의 상호 관련성이 매우 높게 나타났다.

본 연구는 도시재생 선도지역으로 지정된 곳이자 순천부읍성의 중심지역으로 700년의 역사성과 장소성을 지닌 순천 시 문화의 거리를 대상지로 중요도-만족도 매력속성을 분석하여 다음과 같은 결론을 도출하였다. 문화의 거리 중요도와 만족도간의 차이분석에서는 19개 모든 항목에서 기대속성인 중요도와 성취속성인 만족도간의 항목들이 서로 일치하고 있었으며, 중요도에 비해 만족도가 낮게 나타나 방문객의 높은 요구에 대응하기 위해서는 지속적인 개선방안이 요구되 었다. 전체 중요도는 3.65, 전체만족도는 3.17로 평균이상으로 평가되었다. ISA 결과, 중요도와 만족도가 모두 높은 매력속성으로는 접근성, 서비스, 대표성 등으로 나타나 이는 순천시 문화의 거리의 경쟁우위에 있는 강점들로 분석되었고, 전통성, 편의성, 야간이용시 안전성 등은 중요도에 비해서 만족도가 낮은 항목들로 분석되어 도시재생 및 문화의 거리의 활성화를 위해서는 다양한 정책의 변화와 체계적인 관리방안이 필요한 것으로 평가되었다.

The Riptortus-Burkholderia symbiosis is a newly emerging insect-bacterium symbiotic system. This symbiosis system has a good merit as an experimental model system to produce the non-symbiotic (apo) and symbiotic (sym) host insect. In recent reported papers, the symbionts play important biological roles for the host insects. Meanwhile, juvenile hormone (JH) is one of major hormone synthesized corpora allata(CA) to control many physiology of insect. However, the study for cross-talk mechanism between symbionts and host hormones to control important physiological phenomenon of insects is almost none. In this study, we found that Riptortus speed up adult emerging and increase egg laying on presence of symbiont Burkholderia. Also we found that hexamerin proteins, which were controlled the expression by JH, were accumulated in sym-Riptortus hemolymph compare with apo-Riptortus. According as combined results, we hypothesized that the gut symbiont Burkholderia can control JH titer to conclude out beneficial effects such as development and reproduction of R. pedestris. To verify this hypothesis, we examined measurement of JH titer, expression of hexamerins as JH response genes and RNAi for hexamerin protein during whole Riptortus life on presence or absence of symbiont Burkholderia. All results demonstrated that gut symbiont controlled JH titer of Riptortus. Controlled JH amount by symbiont Burkholderia in host midgut regulated hexamerin protein expression for speeding up adult emerging and increasing egg production.

Various insect pests infest crops including vegetables and ornamentals during crop production. Chemical pesticides have generally used until recently to control pests. Many pests are very difficult to control using organic pesticides because of the development of insecticide resistance and their rapid population increase. Entomopathogens are the promising alternative control methods. Entomopathogenic fungi are good candidates to control sucking insects such as aphid, whitefly and mite because the fungi can infect without ingestion. Conidia or blastospores of fungal entomopathogens are sprayed onto target plant and/or insect, adhered on insect surfaces, and penetrated into hemocoel through insect cuticle. Then the fungus utilize insect nutrients and kill the host insect. During this process, fungus was influenced by environmental conditions such as temperature, relative humidity and UV light. These are causing slow mortality and preventing wider application and use of mycopescitide using entomopathogenic fungi. In addition, control efficacy with fungal entomopathogen differed fungal isolate and host insect. Therefore, we need to study selection of high virulence isolate, mass production, formulation and application techniques to develop mycopesticide.

The bumblebee, Bombus terrestris, has played an important role as one of the alternative pollinators since the outbreak of honeybee collapse disorder. Recently, pathogens and parasites such as viruses, bacteria and mites, which affect the life span and fecundity of their host, have been discovered in B. terristris. In order to detect the microsporidian pathogen, Nosema Spp. in the field populations of B. terristris, we collected adults and isolated their genomic DNA for diagnostic PCR. The PCR primers specific for Nosema Spp. were newly designed and applied to gene amplification for cloning. Only small subunit ribosomal RNA(SSU rRNA) gene of N. ceranae was successfully amplified and sequenced among examined genes, which indicates that N. ceranae mainly infects the examined field population of B. terristris. To detect of SSU rRNA gene, two regions of SSU rRNA gene were selected by primary PCR analysis and further analyzed in quantitative real-time PCR(qRT-PCR). The qRT-PCR analysis demonstrated that SSU rRNA of N. ceranae was detected at concentrations as low as 0.85 ng/μl genomic DNA. This result suggests that the detection via qRT-PCR can be applied for the rapid and sensitive diagnosis of N. ceranae infection in the field population as well as risk assessment of B. terristris.

Use of conidia or spores of entomopathogenic fungi are influenced by environmental conditions such as temperature and relative humidity and caused slow and fluctuation of mortality. In addition, although the fungi have the advantage of a restricted host range, this specificity is one of the limiting factors for their use. These factors are preventing wider application and use of these biocontrol agents. To mitigate such problems we selected an Beauveria bassiana Bb08 which kill green peach aphid with its liquid culture filtrate. In this study we conducted bioassay with the fungal culture filtrate and culture fluid to greenhouse pests such as cotton aphid, whitefly and thrips. Culture fluid showed high mortality against green peach aphid, as well as cotton aphid, sweet potato whitefly, and western flower thrips. However, control effect of culture filtrate varied with pests. Culture filtrate showed high mortality to cotton aphid. Mortality of western flower thrips with culture filtrate was slower than culture fluid including spores and sweet potato whitefly was much lower. These results indicated that the culture fluid of Beauveria bassiana Bb08 has potential to simultaneously control various greenhouse pests.

Sweetpotato whitefly (Bemisia tabaci), especially Q biotype, has been recognized one of the most destructive insect pests worldwide because of increased resistance to some insecticide groups requiring alternative strategies for its control. We studied the conidia production of entomopathogenic fungus Isaria javanica Pf04, which had been reported high virulence isolate against Q biotype of B. tabaci, using grain. Brown rice was most suitable for conidia mass production of the isolate of I. javanica. Conidia was produced high at 25 ~ 27.5℃. The isolate produced more spores when conidia suspension directly inoculated onto media than two-phase fermentation. When concentration of inoculum was high spore production was high, but increasing rate of conidia production was highest at low inoculum concentration (1×105 conidia/ml) as 6,700 times increase compared with 20 times increase at high inoculum concentration (1×108 conidia/ml). These results indicated that the isolate can produce more conidia with cheap agricultural product and can develop as a microbial pesticide to control sweetpotato whitefly.

As the demand for large-scale analysis of gene expres- sion using DNA arrays increases, the importance of the surface characterization of DNA arrays has emerged. We com- pared the efficiency of molecular biological applications on solid-phases with different surface polarities to identify the most optimal conditions. We employed thiol-gold reactions for DNA immobilization on solid surfaces. The surface polarity was controlled by creating a self-assembled monolayer (SAM) of mercaptohexanol or hepthanethiol, which create hydrop- hilic or hydrophobic surface properties, respectively. A hyd- rophilic environment was found to be much more favorable to solid-phase molecular biological manipulations. A SAM of mercaptoethanol had the highest affinity to DNA mole- cules in our experimetns and it showed greater efficiency in terms of DNA hybridization and polymerization. The opti- mal DNA concentration for immobilization was found to be 0.5 mM. The optimal reaction time for both thiolated DNA and matrix molecules was 10 min and for the polymerase reaction time was 150 min. Under these optimized condi- tions, molecular biology techniques including DNA hybri- dization, ligation, polymerization, PCR and multiplex PCR were shown to be feasible in solid-state conditions. We de-monstrated from our present analysis the importance of surface polarity in solid-phase molecular biological appli- cations. A hydrophilic SAM generated a far more favorable envi- ronment than hydrophobic SAM for solid‐state molecular techniques. Our findings suggest that the conditions and met- hods identified here could be used for DNA‐DNA hybri- dization applications such as DNA chips and for the further development of solid-phase genetic engineering applicatio- ns that involve DNA-enzyme interactions.