호접란은 세계적으로 분화뿐만 아니라 절화로도 판매되는 주 요 화훼작물이다. 상업적 종묘 대량생산은 조직배양 기술에 의 해 이루어지고 있으나 우리나라는 아직까지 균일하고 우수한 발근묘 생산 기술이 확립되지 않았다. 생육이 고르고 우수한 발근 배양묘를 생산하기 위한 적정 배양 신초수를 찾기 위해 용적 500mL 유리 배양병에 호접란 2품종(‘Lovely Angel’과 ‘UniVivace’)의 신초를 1, 4, 7, 10개씩 배양한 후 3개월에 지상부 및 지하부 초기 생육 특성을 조사한 결과, 7개의 신초를 배양하였을 때 신초의 생육이나 뿌리 유도 및 생육에 효과적이 었다. 또한, 배양병 재질이 발근묘 생육에 미치는 영향을 확인하 고자 용적이 500mL로 동일한 유리 배양병과 플라스틱 배양병 에 신초 7개 배양 3개월 후 2품종의 생육 특성을 비교한 결과, 2품종 모두 플라스틱 배양병 보다는 유리 배양병이 유의적으로 생육에 효과적인 것으로 확인되었다. 기내 발근묘 생산을 위한 배양병 재질의 영향은 이후까지 지속적으로 영향을 미치는 것으 로 확인되었다. 결론적으로, 균일한 호접란 발근묘 생산은 플라 스틱 재질보다는 유리병 재질의 배양병이 적합하며, 특히, 용적 이 500mL인 유리 배양병의 경우 신초를 7개(묘당 재식면적 5.4㎠) 이내로 배양하는 것이 바람직한 것으로 확인되었다.

Haemaphysalis longicornis는 사람과 동물에게 여러 심각한 병원체를 전달하는 주요 매개체로, 한반도에 널리 분포하고 있다. H. longicornis는 Rickettsia spp., Borrelia spp., Francisella spp., Coxiella spp., 그리고 중증열성혈소판 감소증후군 바이러스 (SFTS virus) 등을 매개하는 것으로 알려져 있다. 국내에 서식하는 H. longicornis의 미생물 군집과 관련된 연구는 많이 진행되지 않은 것으로 확인되었다. 이 연구는 한반도 내 다양한 지역에서 채집된 H. longicornis의 미생물군집 다양성을 지역별, 성장 단계 및 성별에 따라 분석하였다. 2019년 6월부터 7월까지 질병관리청 권역별기후변화매개체감시거점센터 16개 지역에서 채집한 H. longicornis의 16S rRNA 유전자 V3-V4 영역을 PCR로 증폭 후 Illumina MiSeq 플랫폼으로 시퀀싱하였다. Qiime2를 활용한 미생물 다양성 분석을 통해 총 46개의 샘플에서 1,754,418개의 non-chimeric reads를 얻었으며, 평균 126개 의 operating taxonmic unit (OTU) 을 식별하여 총 1,398개의 OTU를 확인하였다. 대부분의 지역에서 Coxiella spp.가 우점종으로 나타났으며, 특히 Coxiella endosymbiont는 가장 높은 우점도를 보이며, Coxiella burnetii와 계통 발생 학적으로 유사한 것으로 확인되었다. 이 연구를 통해 분석된 결과는 각 지역의 H. longicornis 미생물군집 데이터 베이스 구축에 활용되었으며, 이를 통해 지역별 미생물군집의 특이성을 식별할 수 있게 하였다. 이는 한반도의 H. longicornis에 의한 질병 전파 연구와 이를 통한 공중보건 개선에 기여할 것으로 기대된다.

갈색거저리 유충의 사료인 밀기울은 대부분 수입에 의존하고 있는데 일부 국가의 식량 수출 중단 조치 등에 따른 국제 곡물가격 상승으로 밀기울 가격은 인상되고, 식용곤충 판매가격이 하락하면서 생산비 절감을 위한 사료 개발이 요구되고 있다. 농업부산물 3종을 50% 이상 급이하게 되면 유충 생육이 저하되었기 때문에 본 연구는 적정한 배합비율을 선정하기 위해 첨가사료 20, 30% 함량으로 밀기울과 혼합하여 사료를 급이하였을 때 갈색거 저리 유충의 생육 특성과 영양성분 변화에 대해 밀기울만 제공한 대조구와 비교하였다. 부산물 A와 B, C를 각각 30% 함유한 처리구에서 갈색거저리 유충 무게는 대조구와 차이가 없음을 확인하였다. 먹이소화율은 부산물B를 20% 함유한 처리구가 80.5%로 대조구에 비해 높았고, 부산물C 30% 처리구에서 72.6%로 가장 낮았다. 갈색거저 리 유충의 생육일수 100일 기준으로 부산물B 30% 처리구에서 용화율이 76.1%로 대조구보다 1.6배 높았으며, 부산물A 20% 처리구는 29.2%로 용화율이 가장 낮았다. 갈색거저리 유충의 조단백질 함량은 부산물 C 30% 처리 구에서 대조구보다 10.3% 증가하여 아미노산 분석을 진행한 결과 sarcosine과 ornithine이 2.5배 이상 증가하였다. 이를 통해 부산물 B나 C를 30% 함유한 사료를 급이하였을 때 사육 원가를 절감하여 유충을 생산할 수 있을 것으로 사료된다.

The purpose of this study is to select various insect species for healing resources and develop a healing program in order to use insect as a healing agriculture. In this study, there are two kinds of breeding kit were developed, one for Gryllus bimaculatus and the other for Oxya chinensis sinuosa. Using these insect breeding kits, we conducted a survey of 60 children and the elderly. In the case of children, the results of the insect breeding satisfaction showed that 30.6% said that the sound of crickets was very good, and 11.1% said that it was good. In addition, the higher the child's awareness of insects, the higher the proportion of children who wanted to raise insects in the future. As a result of a survey of seniors, 45.2% do not like insects and 51.6% are not interested, meaning that most seniors are not very interested in insects. However, the emotions after breeding insects showed positive results, with 45.2% saying their personality became brighter, 48.4% reducing their anger, 48.4% relieving their irritation, 54.8% relieving loneliness, 58.1% feeling more responsible, and 51.6% developing intimacy.

This study investigated species identification and labeling compliance of 48 shrimp products sold in the Korean online markets. Species identification was conducted using the standard DNA barcoding method, using the cytochrome c oxidase subunit I gene. The obtained sequences were compared with those deposited in the NCBI GenBank and BOLD Systems databases. Additionally, phylogenetic analysis was performed to further verify the identified shrimp species. Consequently, 16 shrimp species were identified, including Penaeus vannamei, Pandalus borealis, Palaemon gravieri, Leptochela gracilis, Penaeus monodon, Pleoticus muelleri, Metapenaeopsis dalei, Euphausia pacifica, Lebbeus groenlandicus, Trachypenaeus curvirostris, Argis lar, Metanephrops thomsoni, Metapenaeopsis barbata, Alpheus japonicus, Penaeus chinensis, and Mierspenaeopsis hardwickii. The most prevalent species was Penaeus vannamei, found in 45.8% of the analyzed products. A significant mislabeling rate of 72.9% was found; however, upon excluding generic names such as shrimp, the mislabeling rate reduced to 10.4%. The mislabeling rate was higher in highly-processed products (89.3%) compared with that in minimally-processed products (50%). No correlation was found between the country of origin and mislabeling rate. The results of this study provide crucial data for future monitoring of shrimp products and improving the labeling of shrimp species in Korea.

In this study, based on an analysis of two DNA barcode markers (cytochrome c oxidase subunit I and cytochrome b genes), we performed species identification and monitored labeling compliance for 50 commercial pufferfish products sold in on-line markets in Korea. Using these barcode sequences as a query for species identification and phylogenetic analysis, we screened the GenBank database. A total of seven pufferfish species (Takifugu chinensis, T. pseudommus, T. xanthopterus, T. alboplumbeus, T. porphyreus, T. vermicularis, and Lagocephalus cheesemanii) were identified and we detected 35 products (70%) that were non-compliant with the corresponding label information. Moreover, the labels on 12 commercial products contained only the general common name (i.e., pufferfish), although not the scientific or Korean names for the 21 edible pufferfish species. Furthermore, the proportion of mislabeled highly processed products (n = 9, 81.8%) was higher than that of simply processed products (n = 26, 66.7%). With respect to the country of origin, the percentage of mislabeled Chinese products (n = 8, 80%) was higher than that of Korean products (n = 26, 66.7%). In addition, the market and dialect names of different pufferfish species were labeled only as Jolbok or Milbok, whereas two non-edible pufferfish species (T. vermicularis and T. pseudommus) were used in six commercial pufferfish products described as JolboK and Gumbok on their labels, which could be attributable to the complex classification system used for pufferfish. These monitoring results highlight the necessity to develop genetic methods that can be used to identify the 21 edible pufferfish species, as well as the need for regulatory monitoring of commercial pufferfish products.

Radioactive waste is typically disposed of using standard 200 and 320 L drums based on acceptance criteria. However, there have been no cases evaluating the disposal and suitability of 200 L steel drums for RI waste disposal. There has been a lack of prior assessments regarding the disposal and suitability of 200 L steel drums for the disposal of RI waste. Radioactive waste is transported to disposal facilities after disposal in containers, where the drums are loaded and temporarily stored. Subsequently, after repackaging the disposal drums, the repackaged drums are transported to disposal facilities by vehicle or ship for permanent disposal. Disposal containers can be susceptible to damage due to impacts during transportation, handling, and loading, leading to potential damage to the radiation primer coating during loading. Additionally, disposal containers may be subject to damage from electrochemical corrosion, necessitating the enhancement of corrosion resistance. Metal composite coatings can be employed to enhance both abrasion resistance and corrosion resistance. The application of metal composite coatings to disposal containers can improve the durability and radiation shielding performance of radioactive waste disposal containers. The thickness of radioactive waste disposal containers is determined through radioactive shielding analysis during the design process. The designed disposal containers undergo structural analysis, considering loading conditions based on the disposal environment. This paper focuses on evaluating the structural improvements achieved through the implementation of metal composite coatings with the goal of enhancing corrosion and abrasion resistance.

This study investigated ethopabate (EPB) residues in edible tissues of broiler chickens given in drinking water and established the withdrawal time (WT) of EPB in poultry tissues. Twenty-four healthy Ross broiler chickens were orally administered with EPB at the concentration of 3.8 mg/L for 14 days (EPB-1, n=24) and 15.2 mg/L for 7 days (EPB-2, n=24) through drinking water, respectively. After the drug treatment, tissue samples were collected from six broiler chickens at 0, 1, 3, and 5 days, respectively. EPB residue concentrations in poultry tissues were determined using LC-MS/MS. Correlation coefficient values ranged from 0.9980 to 0.9998, and the limits of detection and quantification (LOQ) were 0.03~0.09 and 0.1~0.3 μg/kg, respectively. Mean recoveries in muscle, liver, kidney and skin/fat tissues were 95.9~109.8, 108.7~115.3, 89.9~96.6 and 86.7~96.8%, respectively, and coefficient of variations were less than 17.11%. At the end of the drug-administration period (0 day), EPB was detected at levels under the LOQ in all tissues from both the EPB-1 and EPB-2 groups. According to the results of EPB residue in Ross broiler tissues, withdrawal periods of both EPB-1 and EPB-2 in poultry tissues were established to 0 day. In conclusion, the developed analytical method is suitable for the detection of EPB in poultry tissues, and the estimated WT of EPB in poultry tissues will contribute to ensuring the safety of Ross broiler chickens.

We investigate the provenance of detrital garnets in Middle-Upper Jurassic sandstone of the Mino terrane, an accretionary complex in Southwest Japan, based on their chemical composition. The garnet grains in the Mino sandstone are mostly Fe-rich (almandine) and slightly Mg-rich (pyrope) species derived from high-grade metamorphic and intermediate to acidic plutonic rocks. The composition and interpreted origin of the garnets are generally consistent with those of metamorphic and igneous rocks of the Yeongnam Massif on the Korean Peninsula, a possible source region suggested in previous studies. In addition, two single grains of chromian spinel, an accessory mineral found in mafic to ultramafic rocks such as mantle peridotite, were found in one of the Mino sandstone samples. This finding suggests the possible presence of mafic to ultramafic rocks in the source area. The results of this study provide complimentary evidence for establishing a comprehensive tectonic and paleogeographical framework for the Mesozoic East Asian continent.

From 2020, Korean Animal and Plant Quarantine Agency has reset the withdrawal time (WT) for veterinary drugs typically used in livestock in preparation for the introduction of positive list system (PLS) program in 2024. This study was conducted to reset the MRL for amprolium (APL) in broiler chickens as a part of PLS program introduction. Forty-eight healthy Ross broiler chickens were orally administered with APL at the concentration of 60 mg/L (APL-1, n=24) for 14 days and 240 mg/L (APL-2, n=24) for 7 days through drinking water, respectively. After the drug treatment, tissue samples were collected from six broiler chickens at 0, 1, 3 and 5 days, respectively. Residual APL concentrations in poultry tissues were determined using LC-MS/MS. Correlation coefficient (0.99 >), the limits quantification (LOQ, 0.3~5.0 μg/kg), recoveries (81.5~112.4%), and coefficient of variations (<15.5%) were satisfied the validation criteria of Korean Ministry of Food and Drug Safety. In APL-1, APL in all tissues except for kidney was detected less than LOQ at 3 days after drug treatment. In APL-2, APL in liver and kidney was detected more than LOQ at 5 days after treatment. According to the European Medicines Agency’s guideline on determination of withdrawal periods, withdrawal periods of APL-1 and APL-2 in poultry tissues were established to 3 and 2 days, respectively. In conclusion, the developed analytical method is sensitive and reliable for detecting APL in poultry tissues. The estimated WT of APL in poultry tissues is longer than the current WT recommendation of 2 days for APL in broiler chickens.

The bacterial soft-rot disease is one of the most critical diseases in vegetables such as Chinese cabbage. The researchers isolated two bacteria (Pseudomonas kribbensis and Pantoea vagans) from diseased tissue samples of Chinese cabbages and confirmed them as being the strains that cause soft-rot disease. Lactic-acid bacteria (LAB), were screened and used to control soft-rot disease bacteria. The researchers tested the treatments with hypochlorous acid water (HAW) and LAB supernatant to control soft-rot disease bacteria. The tests confirmed that treatments with the HAW (over 120 ppm) or LAB (Lactobacillus plantarum PL203) culture supernatants (0.5 mL) completely controlled both P. kribbensis and P. vagans.

In order to extend the business viability of carbon nanotubes (CNTs), research on CNT dispersion in a solvent as well as in polymer matrix should be established. Herein, three kinds of dispersing agents, sodium deoxycholate (DOC), sodium dodecylbenzene sulfonate (NaDDBS), polyvinyl pyrrolidone (PVP), are selected and applied to quantify the dispersibility and dispersion stability of CNT aqueous dispersion. The dispersibility of CNT dispersion with the PVP, evaluated via viscosity and particle size analyses, are superior to those with the DOC and NaDDBS dispersing agents. CNT aqueous solution dispersed with PVP showed slightly higher viscosity and narrower particle size distribution than those with DOC and NaDDBS dispersing agents. In addition, the dispersion stability of CNT dispersion with the PVP, measured via lumisizer analyses, are superior to those with the DOC and NaDDBS dispersing agents. HR-TEM analysis verifies that the outstanding dispersibility and dispersion stability of CNTs in aqueous solution are due to the effect of the robust polymer wrapping of the PVP dispersing agent on the CNT surface. From the results of this study, the guidelines for the selection of the suitable dispersing agents and the systematic evaluation of dispersibility and dispersion stability of CNT dispersions can be suggested.

Colorectal cancer causes the most cancer-associated death worldwide, having a high cancer incidence. Pectin is a complex polysaccharide present in various fruits, emerging as an anti-carcinogenic candidate. Although pectin has a suppressive capacity for colon carcinogenesis, the effect of reactive oxygen species (ROS) generation and colonic aberrant foci formation in the colon carcinogenesis mouse model remains unclear. Therefore, this study investigates the regulatory effect of pectin supplementation on colon carcinogenesis induced by azoxymethane (AOM) and dextran sodium sulfate (DSS) in mice. In an animal experiment, thirty male institute for cancer research (ICR) mice were divided into two experimental groups; AOM/DSS (control group) and AOM/DSS + pectin (5% in drinking water). Furthermore, the number of aberrant crypt foci (ACF) and aberrant crypt (AC) on colonic mucosa were counted, and thiobarbituric acid-reactive substances (TBARS) assay was performed to estimate lipid peroxidation in feces. Pectin treatment significantly decreased the number of ACF and AC per colon compared with the control. Additionally, fecal TBARS level in the pectin group was significantly lower than those in the control group. Conclusively, these findings indicate that pectin-inhibited hyperplastic alteration and oxidative stress suppress colitis-associated colon carcinogenesis.