차세대 염기서열 분석(Next Generation Sequencing, NGS)은 대량의 병렬 데이터 생산으로 유전체의 염기서열 을 고속으로 분석하는 기술이며, 이 기술은 바이러스 유전체 분석에도 광범위하게 사용되고 있다. 하지만, 바이 러스의 전장 유전체가 100kb를 넘을 경우, 동일한 raw data라도 분석 방법 및 소프트웨어 그리고 매개변수 (parameter)에 따라 유전체의 크기와 구조가 다르게 결정된다. 따라서 유전체가 큰 바이러스 분석 시, 최적화된 NGS 분석 방법을 선택하는 것이 중요하다. 본 연구는 장수풍뎅이 누디바이러스(Oryctes rhinocerous nudivirus, 120kb) 유전체를 기반으로, 다양한 Assembly 소프트웨어(metaviralSPAdes, metaSPAdes, velvet, shovill, Geneious, megahit)를 사용하여, 최적화된 NGS 분석 방법을 고안하였다. Assembly 소프트웨어에 따라 바이러스 유전체 크기와 특징(Single Nucleotide Polymorphism, Insertion&Deletion, repetitive genomic variants)의 차이를 확인하였 다. Assembly 소프트웨어 간의 차이가 있는 염기서열은 Sanger sequencing을 통해 재확인하여, 참조 유전체 (reference sequence)를 구축하였다. 이 참조 유전체를 기반으로 가장 정확한 Assembly 소프트웨어와 parameter를 평가하였다. 본 연구는 분석 방법에 따라 달라지는 유전체의 특성을 이해하고, 바이러스 유전체를 정확하게 구축 하는 분석 파이프라인을 제공할 것으로 기대된다.

This study was conducted to investigate whether there were differences in eco-friendly food, home meal replacement (HMR) purchases, and eating-out behavior according to the level of agri-food consumer competence. The data for the study were extracted from main food consumers (n=3,321) in the 2022 Food Consumption Behavior Survey. The competence index was divided into awareness-attitude-practice items, and three groups were classified by competence level. The results showed an agri-food consumer competency score of 70.62, with the highest score for awareness (73.96), followed by practice (69.28) and attitude (66.18). The frequency of purchasing eco-friendly food was higher in the excellent group compared to other groups, and quality and price satisfaction was higher with higher competency (p<0.001). Regarding HMR, the results showed that the shortage group had the lowest HMR consumption rate, and satisfaction decreased as competence decreased (p<0.001). The main reason for eating-out was to enjoy food in all groups (59.0%), followed by a lack of cooking time in the excellent group (15.7%) and hassle with food preparation in the moderate and shortage groups (17.3%, 16.6%) (p<0.001). In short, agri-food consumption competency showed differences by contents and components, and differences in food purchases and eating-out behavior by competency level were found.

Ecological occupation in irrigation ponds is a well-acknowledged fact that is essential for biodiversity conservation in agricultural ecosystems. However, there are few studies on the ecological functions and relationship between a paddy field and irrigation using macroinvertebrates in an environmentally friendly paddy field. The objective of this study is to identify the community and distribution characteristics of macroinvertebrates in an agricultural paddy field and irrigation pond ecosystems, and to provide basic data on the ecological function of an environmentally friendly paddy field. Macroinvertebrate sampling was conducted from May to September in an agricultural paddy field and irrigation pond in an environment-friendly paddy field in Boryeong city. We conducted a study to identify the distribution characteristics using macroinvertebrate species analysis, such as Functional Feeding Groups (FFGs), Habitat Oriented Groups (HOGs), rarefaction curve, and a two-way dendrogram. A total of 37 species of macroinvertebrates in 28 families, 13 orders were collected study during the period of the investigation. Dominant taxa of macroinvertebrates included Coleoptera, Hemiptera, and Odonata. In terms of FFGs, predators and gathering collectors accounted for approximately 70%, in relation to HOGs, and climbers and swimmers occupied more than 50% from both the paddy field and irrigation pond. With respect to the rarefaction curve, the irrigation pond (July) was high as E (S, 141)=18 species, while the paddy field (May) was comparatively low as E (S, 141)=9 species. In conclusion, our results revealed that macroinvertebrates, such as Notonecta triguttata, Peltodytes intermedius, Appasus major, Laccotrephes japonensis, Appasus japonicus, Sigara substriata, Enochrus simulans, and Sternolophus rufipes, were used as a habitat and spawning ground in both paddy field and irrigation pond. The irrigation pond appears to be a very important spawning ground for macroinvertebrates.

본 연구의 목적은 온탕침지와 pH교정석회유황합제 처리에 의한 상추 유기종자의 곰팡이와 세균의 소독효과 및 발아율에 미치는 영향을 검정하고자 하였다. 상추종자에 Alternaria sp.가 53.3% 감염되어 있었고 Aspergillus sp.와 Cladosporium sp.은 각각 14.5%와 5.4% 감염되어 있었다. 세균은 형태적으로 Pseudomonas sp., 한 종만 분리되었으며 16.5%가 감염되어 있었다. 상추종자 소독에 효과적인 온탕침지조건을 탐색하고자 온탕의 온도조건(45℃, 50℃, 55℃, 60℃)에 따른 소독효과를 조사하였다. 온탕침지 온도와 처리시간이 증가함에 따라 곰팡이와 세균에 대한 살균효과는 증가하였으나 종자의 발아율은 급격히 감소하였다. 온탕침지 단독처리는 50℃ 온탕에서 20분간 처리할 경우 상추종자의 살균효과와 발아율이 각각 91.1%로 가장 우수하였다. 상추종자 소독을 위한 온탕침지와 pH교정석회유황합제 교호처리는 50℃의 온탕침지 조건에서는 10분간 처리하는 것이 상추종자의 살균효과와 발아율이 각각 100%와 97.6%로 가장 높았다. 본 연구결과는 종자 표면뿐만 아니라 종자내의 곰팡이나 세균을 살균할 수 있는 기술개발에 기여할 수 있을 것으로 생각한다.

토양에 활용되는 유기농업자재(퇴비, 유황)가 굼벵이 생육 및 생존에 미치는 영향을 알아보기 위하여 생육시험을 수행하였다. 먹이는 참나무발효톱밥을 이용하였으며, 가축의 퇴비와 유황을 일정비율(0.3, 1, 3, 10 %) 섞어 사육하였다. 시험곤충은 흰점박이꽃무지 2령 유충을 사용하였다. 사육 시 나타나는 생장율 차이를 비교하여 흰점박이꽃무지 유충의 발육 특성을 조사하였다. 참나무발효톱밥으로만 사육한 개체에 비해 유황을 혼합한 경우, 유황의 비율이 높아질수록 생중량의 증가율이 낮아지는 것으로 나타났다. 가축퇴비(우분 40%, 돈분 10%, 계분 5%)를 배합하여 사육한 경우, 참나무발효톱밥에 10% 퇴비를 배합하였을 때, 21일 후 생중량이 평균 4.18배 증가하는 것을 확인하였다.

This work studies the variability of flatfish sales revenue. The theoretical analysis draws functions for equilibrium price and quantity using expectation hypotheses. The functions include unpredictable phenomenon with dummy variable and GARCH. The equilibrium function, using adaptive expectation hypothesis, contains the independent variables of supply and demand, while the equilibrium function, embodying rational expectation hypothesis, includes only the independent variables of supply side, because the demand side disappears by the information extraction process theoretically, if economic subjects build the expectation rational. The empirical analysis shows: the variability of flatfish production has a spillover effect on the variability of revenue with the adaptive expectation hypothesis. In the case when the model has a rational expectation hypothesis, the variability of flatfish production has a spillover effect on the revenue (the mean equation of GARCH model). This study indicates that there is the variability in flatfish production and sales revenue, and the spillover effect between them. The result can help to build of the rational system for the fishery income stability.

본 연구는 지구온난화와 식물 생장의 제한요소로 알려져 있는 질소가 단양쑥부쟁이의 생육에 어떤 영향을 미치는지 알아보기 위해 지구온난화 조건하에서 질소의 농도를 다르 게 처리하여 생육시킨 후 그 결과를 비교하였다. 이를 위하 여 온실을 두 구획으로 나누고, 한 곳은 CO2를 처리하지 않은 대조구로, 다른 한 곳은 CO2를 처리한 처리구로 설정 하였다. 대조구는 대기 중 CO2 농도와 온도를 그대로 반영 하였고, 처리구는 대조구보다 CO2 농도와 온도를 더 높게 상승시켰다. 그 후, 대조구와 처리구 내에서 각각 개체수(1, 2, 3 개체)와 질소량(0, 2, 20g)을 다르게 처리하여 지상부, 잎 폭 그리고 잎 길이에 차이를 비교하였다. 그 결과 개체수 가 하나 이거나 두 개일때, 대조구와 처리구 모두 질소량이 증가할 수록 줄기길이, 잎 길이 그리고 잎 폭 모두 좁아졌다. 개체수가 세 개일 때, 질소량이 증가할 수록 대조구는 줄기 길이가 낮아졌고, 처리구는 줄기길이, 잎 길이 그리고 잎 폭 모두 짧아졌다. 일반적으로 단양쑥부쟁이는 하천변에서 군락을 이루어 서식하는 것으로 알려져 있는데, 이 결과는 지구온난화는 거의 영향이 없고, 하천 오염이 심해질 경우 단양쑥부쟁이의 생육에 피해를 입힐 수 있을 것으로 판단된 다.

The purpose of this study was to analyze the post-purchase behavior of customers who experienced cognitive dissonance after exposure to a franchise foodservice advertisement. The study adopted cognitive dissonance theory to explain the dissonance resulting from the combination of advertisements and actual product that consumers received. In detail, the research hypothesized that cognitive dissonance will affect consumers’ post-purchase behavior as well as their efforts to reduce dissonance. Exactly 274 questionnaires were used in the analysis. The results showed that more than 80% of respondents were influenced by advertisements when purchasing menus, and 50% were unsatisfied when the provided menu “was not the same as the advertisement shown.” It was found that advertisement type did not significantly affect either group that experienced dissonance. In terms of satisfaction, however, the former group showed greater dissatisfaction when dissonance occurred (p<.001). Finally, there was a significant difference between the two groups regarding post-purchase behavior (p<.05). Customers with dissonance were likely to ‘express dissatisfaction directly to the store’, ‘leave negative reviews’, and ‘participate in negative word-of-mouth’. Thus, the results revealed that cognitive dissonance could significantly influence customer satisfaction and consequently lead to negative post-purchasing behaviors.

We studied on the diallel cross analysis of three different types of corn(Zea mays L.); supersweet(ss), sticky waxy and normal field corn in Korea and China. Four inbred lines each of three different types were crossed in a full diallel. Testing of 66 F1 crosses were made at two locations in Korea and China, respectively, in 2009. The objectives of the experiment was first to study different levels of heterosis among three types. In Korea, average ear length of 16 F1 crosses was the longest in N x wx (197mm), followed by wx x ss (193mm) and N x ss (191mm). Average of ss, wx and N were 135mm, 171 mm and 155mm. The relatively high level of N might be location specific breeding results, where the testing was done at the center of waxy corn breeding. Trends of three F1 crosses in China were similar with normal and supersweet corn. The result of this study has supported for breeding of high quality sticky-supersweet and high yield potential of normal -supersweet corn hybrids in the developing world to provide nutrition, sugar and other minerals such as Vitamin A and anthocyanin, simultaneously as a high quality food.

Mongolia is known as the livestock country for 800 years from the period of Chinggis Khaan Empire. The livestock has been raised without corn, a high quality feed. Hence, the meat of Mongolia has not been exported. In 2004, International Corn Foundation (ICF) and KNU in Korea initiated corn breeding program for Mongolia with Mongolian Academy of Science and Agricultural University of Mongolia. Since that time approximately 2000 genotypes were planted in Agricultural Research Station in Darkhan, Mongolia. Within 4years of research we have succeeded in forming Mongolian corn population (MCP), which can be used to breed locally adapted varieties in different zones. MCP characterizes early maturity, cold and drought tolerances. In 2008, 160 S1 were planted and 16 lines were selected. For further inbreeding and population improvement we planted all 16 lines in KNU vinyl house at Kunwi in late September. F1 crosses and advanced S3 lines will be tested in 2009 first time for hybrid development in Mongolia.

Super-sweet corn controlled by shrunken2 (sh2) was first introduced into Korea three decade ago, but its cultivation and uses have not been popularized. Main reasons of the un-popularity are considered as lack of locally bred adapted cultivars, low germination, too sweet and less chewing of the green ears. Considering traditional and favorable eating habits of glutinous waxy corn by Koreans, we initiated to develop a double mutant wx/sh corn that has 3:1 segregation in an ear from 1996. First we formed several double cross hybrids using commercial single crosses of wx and sh2 hybrid considering seed production difficulties. The results showed that a double mutant corn hybrid showed high germination, and high palatability score with tolerance to major diseases and insects in Korea. In 2008, we conducted single and double cross trials in Gokseong, South Jeolla-do. The yield of waxy-super sweet was higher than both waxy and super sweet corn with good agronomic traits of ear height, ear weight, ear length and plant height. Among waxy-super sweet corn single cross was even better than double cross in all parameters. This double mutant approach shall be an outstanding option to change of traditional food habits into a new eating pattern. This attempt has potential success of commercial wx/sh hybrid corn development.