본 실험은 저온처리 기간과 일장이 자생 돌마타리의 휴면타파와 생장 및 개화에 미치는 영향을 구명하기 위해 실시되었다. 노지에서 재배중인 돌마타리의 생물계절현상 반응(phenology) 을 3월부터 10월까지 조사하였다. 또한 저온처리 기간이 돌마타리 휴면타파 및 개화에 미치는 영향을 알아보기 위해 4°C 저온에 0, 3, 6, 9, 12주간 처리한 후 장일환경(16h), 25°C 온도조건에서 재배하면서 생육특성을 관찰하였다. 일장이 돌마타리의 생육 및 개화에 미치는 영향을 알아보기 위해 자연저온 처리된 돌마타리를 단일(9h), 중일(12h), 장일조건(16h)에서 재배하여 생육 특성을 관찰하였다. 야외에서 재배중인 돌마타리는 늦여름인 8월부터 9월까지 개화하는 특성을 보였다. 저온처리 기간에 따른 실험결과 9, 12주 저온처리를 받은 식물이 3, 6주간 저온처리 한 식물에 비해 개화소요일수가 유의하게 단축되었다. 돌마타리는 4°C 온도에서 9주 이상 처리시 33%의 개화율을 보이는 것으로 조사되었다. 일장에 따른 실험결과, 장일환경에서 재배된 돌마타리의 초장은 50.1cm였으나 단일 및 중일환경에서 재배된 식물의 초장은 각각 16.7, 16.1cm로 조사되었다. 돌마타리는 장일환경에서 16주간 재배 시 100% 개화하였으나 단일 및 중일 환경에서 재배 시 전혀 개화하지 않았다. 결론적으로 돌마타리 는 휴면타파 및 개화를 위해 저온을 필수적으로 요구하며 장일 환경조건에서만 개화하는 질적장일식물임을 확인하였다.

The Gwangneung arboretum, affiliated to the Korea Forest Research Institute, was established in 1987 and it became the Korea National Arboretum (KNA) in May 24, 1999. The entomological collection of KNA currently holds a total of 510,365 specimens of 6,849 species including more than 330 type specimens of Lepidoptera, Hemiptera, Hymenoptera, and Coleoptera are deposited. As the results of recent systematic studies by KNA entomologists, 67 new species were described, and 108 species were newly recorded from Korea and other countries. Not only we have conducted taxonomic studies but also have broaden our research boundary to natural enemy surveys for pine wilt disease vectors, plant and insect interaction, and exotic insect species monitoring. In this presentation, we would like to briefly introduce current entomological researches of KNA in the hope of future collaborations with other entomologists.

Photosynthetic characteristics and growth responses of Phalaenopsis Queen Beer ‘Mantefon’ orchid were determined in plants exposed to variable carbon dioxide (CO2) concentrations at 2-, 24-, and 36-weeks age (i.e., corresponding to juvenile, young, and mature vegetative growth stages, respectively). Plants were grown at 400 (control), 800, or 1,600 μmol･mol-1 CO2 for 6 hours during the nighttime for 32 weeks. Phalaenopsis ‘Mantefon’ in 2- and 24-week-old plants grown at 1,600 μmol･mol-1 CO2 had increased leaf number and net CO2 uptake compared with the plants grown at 400 μmol･mol-1 CO2. In 36-week-old of Phalaenopsis ‘Mantefon’, leaf number was significantly greater in plant grown at 800 and 1,600 μmol･mol-1 conditions compared with plants grown at 400 μmol･mol-1 CO2. Leaves that emerged after the start of the CO2 treatment were initially longer in the plants grown at 1,600 μmol･mol-1 CO2 than at 400 μmol·mol-1 C O2, but the final leaf length was shortest in the plants grown at 1,600 μmol･mol-1 CO2 condition. Plants showed crassulancean acid metabolism characteristic of nighttime CO2 uptake regardless plant growth stages. We found that growers may be able to promote leaf growth with increasing leaf number and reducing time to leaf initiation in the 36-week-old (i.e., mature stage) plants with 800 – 1,600 μmol·mol-1 CO2 and 2- and 24-week-old (i.e., juvenile and young stages) plants with 1,600 μmol·mol-1 C O2 for Phalaenopsis ‘Mantefon’.

농산물 검역통계를 통해서 채소 모종 수출 현황과 채소 접목묘의 일본 선박 수송 시의 온도 및 상대습도 변화를 분석하여 국내 채소 모종 수출 연구와 정책 수립을 위한 기초자료를 제공하기 위하여 본 연구를 수행하였다. 2007~2016년 사이 수출된 채소 모종은 배추, 양배추 등 엽채류 4종, 토마토, 오이 등 과채류 7종, 근채류 1종으로, 전세계 20여 국가에 46,146,536주(총 1,839건) 수출 되었다. 상업적 목적을 위한 채소 모종의 주요 수출국은 일본이었고, 2016년 기준 일본에 수출된 모종은 주로 과채류 접목묘인 가지, 토마토, 오이, 수박 및 고추 등으로 총 2,575,446주가 수출되었으며, 총 수출 금액으로는 0.7~1.6백만불로 추산된다 일본으로 수출되는 접목묘는 도시농업용과 농가용으로 구분되고 있으며, 온도 15~18oC의 냉장 컨테이너를 이용한 선박 수송은 포장 → 선적 → 검역(한국 부산항) → 검역(일본 시모노세키항) → 바이어 수령의 과정으로 대략 24시간 내외가 소요되고 있었다. 일본의 도시농업 발달로 인한 채소 모종 수요의 급격한 증가와 러시아 등 인근 국가들의 채소 접목묘에 대해 높아지고 있는 관심과 수요는 우리나라 채소 접목묘 수출 규모를 확대할 수 있는 기회가 될 것이다. 채소 모종 수출을 확대하기 위해서는 본 연구 결과를 기반으로 정확한 채소 모종수출 정보의 수집 및 분석이 이루어질 수 있도록 정부의 적극적인 정책 지원과 수출 전용 모종 생산, 모종 저장 및 수송 관련 기술 개발을 위한 연구가 필요하다.

Endoplasmic reticulum (ER) stress is well known as a suppressor in osteoblast differentiation and activating transcription factor 3 (ATF3) could be induced by a various extracellular signals including cytokines, hormones, DNA damage. Up to date, although the role of ATF3 have been studied, the function of ATF3 in osteoblast differentiation is still not clear yet. Our study showed that expression level of ATF3 could be incresed by tunicamycin which is ER stress inducer in preosteoblasts. BMPs, which are secreted by osteoblasts, can be important regulators in osteogenic differentiation. The stress-responsive transcription factor ATF3 is a negative regulator of osteoblast differentiation in MC3T3-E1 cells. In this study, we verified that BMP2-stimulated osteoblast differentiation could be inhibited by over-expressed ATF3 through regulating alkaline phosphatase (ALP) expression and activation.

PURPOSES : Thermal cracking (also called low-temperature cracking) is a serious stress for asphalt pavement, especially in eastern South Korea, the northern USA, and Canada. Thermal cracking occurs when the level of thermal stress exceeds the corresponding level of low temperature strength of the given asphalt materials. Therefore, computation of thermal stress is a key factor for understanding, quantifying, and evaluating the level of low-temperature cracking resistance of asphalt pavement. In this paper, two different approaches for computing thermal stress on asphalt binder were introduced: Hopkins and Hamming’s algorithm (1967) and the application of a simple power-law function. All the computed results were compared visually; then the findings and recommendations were discussed. METHODS: Thermal stress of the tested asphalt binder was computed based on the methodology introduced in previous literatures related to viscoelastic theory. To perform the numerical analysis, MATLABTM 2D matrix-correlation and Microsoft Excel visual basic code were developed and used for the function fitting and value-minimization processes, respectively. RESULTS : Different results from thermal stress were observed with application of different computation approaches. This variation of the data trends could be recognized not only visually but also statistically. CONCLUSIONS: It can be concluded that these two different computation approaches can successfully provide upper and lower limits (i.e. boundaries) for thermal stress prediction of a given asphalt binder. Based on these findings, more reliable and reasonable thermal stress results could be provided and finally, better pavement performance predictions could also be expected.

Pesticide application in agriculture provides significant benefits such as protection from disease, prevention of harmful insects, and increased crop yields. However, accurate toxicological tests and risk assessments are necessary because of many related adverse effects associated with pesticide use. In this review, we discuss and analyze residual pesticides contained in livestock feed in Korea. A pesticide residue tolerance standard for livestock feed has not been precisely established; so, risk assessments are required to ensure safety. Standards and approaches for animal criteria and appropriate methods for evaluating residual pesticides are discussed and analyzed based on technology related to animal product safety in Korea. The safety of livestock feed containing pesticides is assessed to establish maximum residue limits relative to pesticides. Analysis of residual pesticides in milk, muscle, brain, and fat was performed with a livestock residue test and safety evaluation of the detected pesticide was performed. Efficacy of organic solvent extraction and clean-up of feed was verified, and suitability of the instrument was examined to establish if they are effective, rapid, and safe. This review discussed extensively how pesticide residue tolerance in livestock feed and hazard evaluation may be applied in future studies.

본 연구는 기존에 재배된 바 없었던 암대극을 새로운 관상식물로 개발하고자, 절화 특성 평가를 실시하였다. 2017년 3월과 4월, 제주도에 자생하고 있는 암대극을 채취한 후, 수분흡수, 절화수명, 노화 과정 등 절화수명 특성을 평가하였다. 또한 sucrose 농도, 절화길이, pH 농도, 저장온도에 따른 절화 특성을 조사하였다. 본 실험은 국립수목원 유용식물증식센터의 Phyto-garden system(12h photoperiod, 25℃, 70% RH)과 실온(23℃, 44% RH)에서 수행하였고 2~3일 간격으로 수분흡수량, 생체중, 절화수명을 측정하였다. 실험 환경에 따른 연구에서 실온(14.4일) 보다 phyto-garden system(42.4일)의 절화 수명이 약 3배 더 길었고, 절화길이에 따른 연구에서는 절화수명이 20cm, 15.2일, 40cm, 17.4일로 나타났다. Sucrose 농도에 따른 연구에서는 처리에 따른 절화수명 증진 효과는 없었고, pH에 따른 연구의 절화수명은 pH 5, 15.6일, pH 6, 13.4일, pH 7, 13.1일, 증류수, 14.8일이었다. 저장온도에 따른 실험에서, 절화수명은 4℃, 83일, 10℃, 41.2일, 15℃, 35.5 일, 20℃, 17.4일, 실온, 14.4일이었는데, 온도가 감소할수록 절화수명이 길어짐을 알 수 있었다. 결론적으로, 암대극은 비교적 광도와 습도가 높은 환경과 절화길이가 길고 저장온도가 낮을수록 수명이 오래 유지되는 것을 알 수 있었다.

암대극(Euphorbia jolkinii Boiss.)은 우리나라 제주도 및 남부 해안가 암석지에서 자생하는 대극과(Euphorbiaceae)의 다년생 초본식물이다. 등잔모양꽃차례가 황록색으로 3월부터 5월까지 긴 기간 동안 개화하기 때문에 새로운 관상식물로 개발할 가치가 높다. 본 연구에서는 암대극을 재배화하고자 우선적으로 제주도의 자생지 환경과 개체군 분포 특성을 조사하였다. 제주도 전역의 해안을 따라서 암대극 개체의 전수조사를 실시한 결과 40여 지점에 걸쳐 약 14,817개체가 자생하는 것으로 확인되었다. 그 중 제주도 본섬의 동쪽에 위치한 우도에 1,075개체(전체의 7.3%), 북서쪽에 위치한 비양도에 952개체(6.4%), 남쪽에 위치한 가파도에 589개체(4.0%)가 조사되었다. 암대극은 주로 제주도 본섬 남동부 해안가 암석지대에 집중적으로 자생하는 것으로 확인되었고 가파도, 우도, 비양도의 경우에도 섬 동쪽지역에만 분포되어 있었다. 암대극의 생육은 자생지에 따라 차이가 있었다. 초장, 줄기 길이, 줄기 직경은 꽃 크기와 정의 상관관계를 보였다. 모든 지역에서 꽃이 피는 시기는 3월 말로 비슷하게 개화가 시작되었고, 6월 초에는 대부분의 꽃에서 종자가 성숙하여 탈리되었다. 암대극의 주변 식생은 갯개미자리, 돌가시나무, 순비기나무, 갯강활 등 30여 종이 분포되어 있었고, 초본이 80%를 차지하는 데에 반해, 목본은 20%로서 초본식물이 주를 이루었다. 토양의 pH는 8.3~9.4의 범위에 있었으며, 염분 농도는 0.003~0.017%로 나타났다. 유기물 함량은 중문에서 1.9%, 가파도에서 1.4%, 표선면에서 1.0%로 나타났고, CEC는 가파도에서 10.7cmol+/kg, 표선면에서 5.6cmol+/kg, 중문 지역에서 1.3cmol+/kg로 나타났다. 전기전도도는 중문, 가파도, 표선면에서 각각 0.47dS/m, 0.19dS/m, 0.16dS/m으로 나타났고, Cl-은 57.45mg/kg, 63.01mg/kg, 8.12mg/kg으로 나타났다. 중문에서 유효인산은 검출되지 않았다.

Feeding effects of the honeybee pollen products from both domestic, China, Spain and mixture of different origin on the colony development of earth bumblebee, Bombus terrestris L., were surveyed to evaluate efficient nutritional resources for commercial bombiculture in Korea. As the results, the domestic pollen was most effective to achieve high rates of oviposition (88%), colony foundation (70%), and queen production. While feeding with domestic pollens during the egg-laying period, and domestic+Chinese mixture (5:2) during the breeding period (Plot-2), it revealed high oviposition rate of 75%, colony foundation of 65%, and large numbers of adult queen production, indicating its suitability for generation subculturing. In the Plot-3, the same high oviposition rate (75%) was obtained except for feeding with the domestic+Chinese mixture (2:5) during the breeding period, which produced large number of workers.

Development and fecundity of the foxglove aphid, Aulacorthum solani (Hemiptera: Aphididae) were investigated at ten constant temperatures (photoperiod: 15L:9D) from 2.5 to 30℃ on soybean (Glycine max) leaves. The nymphs couldn’t emerge to adults at 2.5 and 30℃. The lower development temperature threshold and thermal constant of development completion estimated with a linear development model in nymph were 5.02℃ and 131.2 degree-days. The lethal temperatures were estimated as 33.9 and 32.5℃ with Lactin 2 and Logan 6 non-linear models, respectively. Mean generation time (from 78.4 to 11.8 d) decreased with increasing temperatures (from 7.5 to 27.5℃). The highest net reproductive rate (77.4) was observed at 20℃. The highest intrinsic rates of population increase (0.282) and shortest population doubling times (2.07 d) were recorded at 25℃.

This study was conducted to develop a simple volume model for the Larix kaempferi species in the Central Region of South Korea. General volume model forms were used and validated using a subset of the data collected for the L. kaempferi species. A total of 550 trees were collected in the different L. kaempferi stands through destructive sampling. The dataset was randomly divided into two: 80% for initial model development(seven volume models) and 20% for model validation. The 100% dataset was used for the final model fitting. Evaluation statistics including bias(Ē), absolute mean deviation(AMD), root mean square error(RMSE), coefficient of determination(R2), and the Akaike Information Criterion values(AIC) and weight (AICw) were used to assess performance of the different volume models. Rank analysis was employed and the first five best volume models were chosen for the model validation and final model fitting. The evaluations showed that volume model 4(V=aDBHb*Hc) had the best performance, while volume model 3(V=aDBH+bDBH2), which has a single variable, had the poorest performance.

The Chinese fashion market, which has typically been dominated by luxury brands, is expanding more into mid- to low-end fashion brands including global SPA (Specialty retailer of private label apparel) brands. This change in the Chinese fashion market is due to the growth of the middle class in China. The advance of the Chinese middle class is attributed to the fact that the wages of large city workers is improving due to the development of various industries, particularly IT. Recently, the Chinese government has initiated an anti-corruption movement, which has led to the prohibition of luxury goods consumption, and so the interest of Chinese potential fashion consumers has moved to the mid-to low-end fashion products imported from developed countries. In addition, young Chinese consumers' positive attitude toward online shopping is favorable for foreign mid-to low-end brands. These changes in the tastes of Chinese fashion consumers are favorable for Korean fashion brands in particular for several reasons. First, the Korean fashion industry does not have a history of producing luxury brands, but does produce many good mid- to low-end fashion brands. In addition, Chinese tend to consider Korean fashion and food culture as more developed and preferable due to the success of the Korean wave. Lastly, it is easy for Korean practitioners to offer online shopping and customer service. The emergence of mid- to low-end fashion brands started in Korea in the late 2000s when global SPA brands entered the market. Unlike Korea's soho brands, which are based on the "No-brand" strategy, global SPA brands have actually shaken up the market by offering reasonable prices for Korean domestic brand products that are of similar quality. Since then, consumers’ willingness to pay for clothing has been reduced even further in the Korean market even though the low-growth trend in the economy has stabilized. So, it is very important to analyze and study the consumption tendency for low and mid-priced fashion brands among both Chinese and Korean consumers. The purpose of this study was to investigate how Chinese and Korean consumers perceive the personality of mid- and low-priced fashion brands and how such personality affects brand loyalty, commitment, and perceived quality. Brand personality is a vital area of research since fashion products are a type of consumer good that is consumed by users close to the body and which can express the self and the personality to others. Aaker (1997) developed a brand personality measurement scale consisting of five dimensions which many researchers have used in exploring the brand personality dimension in various industries. In the study of fashion in particular, the dimension of brand personality has been explored and its effects have been analyzed. However, most of the research has focused on luxury or high priced brands. Also, the research has not compared cultures such as China and Korea in this regard. Therefore, this study explored how Chinese and Korean consumers perceive some of the mid- to low-end priced brands originating in Europe and Korea. Also, the research examined how multiple dimensions of brand personality affected perceived quality, commitment, and brand loyalty. This study assumed that perceived quality and commitment may mediate the relationship between brand personality dimensions and loyalty. In order to conduct quantitative research, this study adopted scales from the previous literature to measure the variables used in the research model. A professional online research company conducted the survey, which was designed to be administered only to potential consumers of mid-and low-priced fashion brands who had purchased one or both European and Korean brands which were suggested in the survey. Also, gender (female), residential area (Seoul, Beijing, Shanghai), and age (21-39 years old) were controlled for. In this research, the European origin brand was the global SPA (Zara, H & M, ONLY), and there were eight Korean brands, including “Style Nanda,” which is popular in China (Refer to Table 1). The data collected on the 250 Korean and 250 Chinese participants were analyzed using statistical package SPSS 20.0. The brand personality dimension was explored using factor analysis which applied Varimax rotation based on the principle component method. As a result, even though the items included were found to be somewhat different according to the country and origin of the brand, the brand personality dimensions of the European global brands turned out to be similar and reflected “TRENDY, SINCERITY, AND COMPETENCE.” Also, Chinese consumers’ perception of brand personality in Korean brands was found to be similar to their perception of European brands. However, Korean consumers’ perception of Korean brands, which are of course their own brands, was more diverse reflecting “TRENDY, SINCERITY, COMPETENCE, AND OUTGOING.” This seems to be because Korean consumers may have had more chance to experience Korean brands either online or offline through visiting stores or being exposed to a variety of marketing communications. Thus, because there are more Korean brands than European, Korean consumers may be able to have an accurate perception of the personality of Korean brands. The conceptual model of the current research includes the relationships among multiple dimensions of brand personality in perceived quality, commitment, and loyalty. Also, the mediating effect of quality and commitment between brand personality and loyalty was examined. In order to test the hypotheses, hierarchical multiple regression using SPSS was analyzed and is described in Table 2 in relation to European brands and in Table 3 in relation to Korean brands. As expected, brand personality dimensions that were found to be a significant factor in perceived quality, commitment, and loyalty were likely to vary based on Chinese or Korean perceptions. For Korean consumers’ perception of global brands, while TRENDY was likely to be the most powerful personality dimension forming brand loyalty, its impact on loyalty seemed not to be mediated by quality but rather by commitment. Also, the influences of COMPETENCE on loyalty were mediated by quality and commitment. For Chinese consumers’ perception of global brands, the impacts of SINCERITY as well as TRENDY on loyalty were found to be mediated by quality and commitment. In addition, COMPETENCE seemed to have a rather direct impact on quality, commitment, and loyalty without a mediating effect. For local brands, Korean consumers did not seem to rely on brand personality or commitment when considering brand loyalty. Since the R2 did not improve at all with adding mediating factors such as quality and commitment, there was found to be no mediating effects in the Korean cases for local brands. In particular, while TRENDY directly impacted loyalty, OUTGOING was found to be significant only in its impact on quality. On the other hand, all three dimensions of local brand personality turned out to be significant influencers on loyalty and quality for Chinese consumers. The impact of TRENDY and COMPETENCE on loyalty seems to be mediated by quality and commitment. In this study, we found that mid- to low-end fashion brands seemed to reflect valid brand personality according to the brand’s origin (global vs. local) as well as consumers’ culture (Chinese vs. Korean). Also, the effect of brand personality dimension was different. This study contributes to the study of brand personality and the Chinese fashion market by comparing a conceptual model of the consumption behavior of Chinese and Korean consumers in relation to mid and low-priced fashion brands and brand awareness formation. In particular, the research revealed differences between Chinese and Korean consumers, suggesting different approaches for Korean fashion practitioners who are planning on entering China, which has not only a similar market to that of Korea but also one that is familiar to Korean practitioners.

We performed a survey for flavivirus infection and distribution of Aedes albopictus that known as Zika and Dengue virus vector using black–light trap and BG-sentinel trap around urban area in Korea. Mosquitoes were collected in 27 cities during March to November (twice a month) year 2016. Total numbers of mosquitoes collected 102,102 including 19 species 8 genera during collecting period. Total 21,467 Ae. albopictus was collected that 20,961(24.3%) by BG-sentinel trap and 506 (3.2%) by Black-light trap in urban area. Trap index(trap/night) of Ae. albopictus was showed highest in Hamyang (TI:992.3) and lowest in Taebaek (TI:0.3) there was only collected by Black-light trap. A total of 894 pools from all collecting Ae. albopictus were performed a Flavivirus detection. Flavivirus was not detected during study period. This study may provide basic information for surveillance of imported diseases (include Zika virus) and vectors in Korea.

Maruca vitrata larvae feed on flowers and pods of several leguminous crops, and can severely reduce seed yield. Adult emergence, mating and oviposition behaviors were observed in a 15h/9h=light/dark and 25℃ condition as a base study for monitoring. Emergence occurred mainly during the first five hours of scotophase. Mating occurred from the two days after emergence, and the mated females started to lay eggs from the next day. A maximal mating rate was observed in the night of five days. Mating occurred significantly more often during the time from 2 hours before to 3 hours after lights-off, but older females mated more frequently during the photophase. Electroanntennographic responses of males to some chemicals, and body extracts and volatile collections of females were measured

The α-Gal epitope (Galα1,3Galα1,4GlcNAc-R) is responsible for hyperacute rejection (HAR) during transgenic pig-to-non-human primate xenotransplantation. To overcome HAR after xenografts, it is essential for the inactivation of α1,3Galactosyltransferase (GT) gene by the homozygotic knocked out of GT-/- and the isoglobotrihexosylceramide synthase (iGb3s-/-). This study was performed to investigate the generation and characterization of the α1,3GT-MCP/-MCP+iGb3-/- transgenic cells. Ear fibroblast cells from the GT-MCP/-MCP pig were cultured and used to positive control. For iGb3s knock out, the Cas9-GFP-iGb3s vector was transfected into the GT-MCP/-MCP cells. The Cas9-GFP-iGb3s transfected cells were sorted and sequenced for the selection of GT-MCP/-MCP+ iGb3s-/- cells. Among the three sorted cell lines, one transgenic cell line was homozygously deleted 3 bases and 10 bases in each chromosome, respectively. To characterize an expression of α-Gal epitope, a wild type and the transgenic cells were measured by FACS Aria using BS-IB4 lectin antibody. The expression of α-Gal epitope in GT-MCP/-MCP cells (<0.01 %) were significantly down-regulated to the range of wild type (99.4 %) fibroblast cells (p<0.05). To analyze the function of iGb3s, α -Gal epitope expressions were measured for the GT-MCP/-MCP, GT-MCP/-MCP+iGb3s-/+, and GT-MCP/-MCP+iGb3s-/-. The range was 95.8%, 94.2%, and 75.8%, respectively. Interestingly, there was a negative range (16.2%) of α-Gal epitope -/- section in GT-MCP/-MCP+iGb3s-/-, compared to 2.74% of GT-MCP/-MCP+iGb3s-/+ and 1.4% of WT, respectively. Our results demonstrated that iGb3s-/-combined with GT-/- had a function to inhibit α-Gal epitope expression in pig cells. Further studies are needed to evaluate the functions of double gene knock out to minimize a HAR response after xenotransplantation.

The α-Gal epitope (Galα1,3Galα1,4GlcNAc-R) is responsible for hyperacute rejection (HAR) during transgenic pig-to-non-human primate xenotransplantation. There are genes related to the expression of α-Gal epitope such as α1,3Galactosyltransferase gene (GT-/-) and the isoglobotrihexosylceramide synthase (iGb3s-/-). This study was performed to investigate the expression of α-Gal epitope in the skin derived from GT-/- transgenic pig. The skin (7/1000 inches) was obtained by dermatome (Zimmer® Electric Dermatome) from one month old of wildtype (WT) and GT-/- piglets, respectively. The skins were fixed, dehydrated, cleaned, and embedded. To analyze the expression of α-Gal epitope, the paraffin section of WT and GT-/- were stained with BS-IB4 lectin and isoglobotrihexosylceramide synthase antibody. There was a strong BS-IB4 lectin signal in the skin of WT, but not detected in GT-/-. However, the iGb3s positive signals were stained in the skin of both WT and GT-/-. Taken together, it can be postulated that the knocked out of GT gene may not enough to inhibit the expression of α-Gal epitope. Further studies are needed to evaluate the functions of the double knock out of GT and iGb3s on the expression of α-Gal epitope.