Excessive intake of sodium caused by high salt diet promotes the expression of inflammatory cytokines and differentiation of helper T cells resulting in inflammatory responses. High-glucose diet also contributes to the pathogenesis of periodontitis by inducing changes in the oral microbiome and reducing salivation. However, the effect of a high-salt and glucose diet (HSGD) on the prognosis of periodontitis remains unclear. In this study, a rat model of experimental periodontitis was established by periodic insertion of absorbable sutures containing Porphyromonas gingivalis and Fusobacterium nucleatum strains into the right gingival sulcus to analyze the effect of HSGD on the incidence and progression of periodontitis. The alveolar bone heights (ABH) was measured with microcomputed tomography imaging of the HSGD- and general diet (GD)-treated groups. The right ABH was significantly decreased compared to the left in both groups at 4 weeks after induction of inflammation; however, no significant difference was noted between the groups. Notably, the ABH in the HSGD-treated group was significantly decreased at 8 weeks after induction of inflammation, whereas in the GD-treated group, an increase in the ABH was observed; a significant difference of the ABH was noted between the two groups (p < 0.05). At 12 weeks, recovery of the alveolar bone was observed in both groups, with no significant differences in ABH between the two groups. These findings indicate that the intake of excessive sodium attenuates the recovery rate of the alveolar bone even after the local infectant is removed. In addition, this study demonstrates the use of HSGD in establishing a new animal model of periodontitis.

Excessive body weight gain during the growth period of early life may predispose individuals towards obesity and metabolic disorder in later life. We investigated the possibility of using the food efficiency ratio as an early indicator for predicting susceptibility to diet-induced obesity and insulin resistance. Four-week-old, prepubertal, male Sprague Dawley rats were divided into obesity-prone and obesity-resistant groups based on food efficiency ratio values after five days on a high-fat diet. Metabolic parameters measured after 2, 6, and 10 weeks, and specific phenotypes were compared with each group. Obesityprone rats had higher increases in body weight and fat mass compared to obesity-resistant rats over the study period. Obesity-prone rats became glucose intolerant early in this study and remained so throughout the experimental period, with increases in fat weight and leptin levels occurring first, followed by increases in insulin level. Gluconeogenesis and insulin resistance significantly increased in obesity-prone groups in which activities of glucose-6-phosphatase and phosphoenolpyruvate carboxykinase were increased and glucokinase activity decreased. Higher food efficiency ratio at an early age was closely correlated with body fat accumulation, hyperleptinemia, and hyperinsulinemia of middle and elderly age. We suggest a high food efficiency ratio in prepubertal subjects may be a useful predictor of future obesity and insulin resistance.

에너지 음료는 카페인을 주성분으로 타우린, 비타민 같은 다른 energy-enhancing 성분을 함유하고 있다. 미국과 유럽에서는 글루쿠로노락톤이 에너지 음료에 첨가될수 있으나, 국내에서 의약품으로는 허가되어 있다. 따라서 식품 첨가물로는 그 사용이 금지 되어 있어, 지속적으로 수입 및 유통 음료에서 시험검사를 하여 규제하고 있다. 현재 분석법으로 사용하는 LC-PDA 법은 복잡한 유도체화 과 정을 거치고, 음료 중에 당류들이 위양성 결과를 나타내 기도 한다. 이런 기존 방법의 단점을 개선하기 위해 HILICESI- MS/MS (hydrophilic interaction liquid chromatography coupled to electrospray ionization tandem mass spectrometry) 를 이용한 분석법을 개발하고, 선택성, 직선성, 검출한계, 정량한계, 정밀도, 정확성, 재현성에 대하여 분석법 유효성 검증을 수행했고, AOAC, EURACHEM 가이드라인에 부합되는 결과를 얻었다.

We determined the effects of different water temperatures (15, 20, and 25℃) and photoperiod cycles (24L:0D, 12L:12D, and 0L:24D) on the oxygen consumption of the offspring of a cultured Japanese strain (JJ), a selected Korean strain (KK), and intraspecific hybrid strains (JK and KJ) of red seabream, Pagrus major, under starvation conditions. The different fish strains, water temperatures, and photoperiod cycles had effects on the mean oxygen consumption of fish. Oxygen consumption increased with increasing water temperatures for all photoperiod treatments (p <0.001). Fish held in continuous darkness (0L:24D) used consistently less oxygen than fish exposed to continuous light (p <0.05). The oxygen consumption of fish exposed to the light phase in a 12L:12D photoperiod was higher than that of fish in the dark phase of the 12L:12D cycle, and differences were significant in three of the strains: JJ (15℃), KK (15 and 20℃), and KJ (25℃). The oxygen consumption of the inbred (JJ and KK) and intraspecific hybrid (JK and KJ) strains varied with differing water temperatures and photoperiod cycles. The JK strain displayed significantly higher oxygen consumption than the other strains under all experimental conditions except 15℃ with a 0L:24D photoperiod. The JK and KJ strains usually showed the highest and lowest oxygen consumption values, respectively, whereas the inbred strains exhibited intermediate values. Oxygen consumption in the JJ and JK strains was usually higher than that of the KK and KJ strains. We propose that differences in the thermal sensitivity and photosensitization properties of the strains contribute to differences in their ability to adapt to changes in water temperature and photoperiod, thus resulting in differences in the amplitude of their metabolic rates.

The purpose of this study was to identify the new fungal disease of Scopendra subspinipes mutilans (Chilopoda: Scolopendridae) from Jeju island in Korea. Scopendra subspinipes mutilans are also known the Chinese red head and average 20cm in length. It is used for traditional medicine for skin-disease and tumor and S. s. mutilans is recently raised in Jeju island to use healing properties. We isolated fungi from surface of infected S. s. mutilans and incubated in SDBA at 24℃ for 14days, and then identified the morphology of the fungi by light and electron microscopy. For the specific diagnosis of Metarhizium spp. in S. s. mutilans, 18srRNA of fungi was amplified by primers ITS4_MF and LR3_MR and sequenced by using the BLAST server at the National Center for Biotechnology Information. The result of the 18srRNA sequencing alignment was Metarhizium anisopliae (99.9%). The entomopathogenic fungi, M. anisopliae would be fatal cause of higher mortality to S. s. mutilans, and it is needed in vivo assay to confirm the pathogenic activities of M. anisopliae to S. s. mutilans.

신뢰성 해석을 수행할 때 정보부족으로 인해 발생하는 인식론적 불확실성(epistemic uncertainty)은 고유의 변동성에 의해 존재하는 내재적 불확실성(aleatory uncertainty)보다 더 중요하게 다뤄야 한다. 그러나 그동안 개발된 확률이론은 주로 내재적 불확실성을 모델링하는데 이용된 반면, 인식론적 불확실성의 모델링에 대해서는 아직 확실한 접근법이 없었다. 최근 이를 위해 probability theory를 포함한 여러 접근법들이 제시되고 있지만 이들은 서로 다른 통계적 이론들을 바탕으로 도출되었기 때문에, 각 방법들의 결과들을 이해하는데 어려움이 있었다. 본 연구에서는 고장 확률을 계산하는 문제를 가지고 이러한 방법들이 인식론적 불확실성을 어떻게 다루는지를 비교, 분석하였다. 이를 위해 probability method, combined distribution method, interval analysis method 그리고 evidence theory를 대상으로 신뢰도 분석문제에 대해 각 방법들의 특징들을 비교하였으며, 그 결과는 다음과 같다. 입력변수의 확률분포 형태를 알 수 있다면 probability method가 가장 우수하나, 이를 전혀 모르면 interval method를 사용해야 한다. 그러나 계산비용 면에서는 두 방법이 유사하므로 결국 입력변수의 확률특성 정보가 얼마나 있느냐에 따라 방법을 선택한다. Combined distribution method는 failure probability의 평균만 제공하므로 사용하지 않는 것이 좋다. 다만 이 방법은 계산비용이 매우 적게 드는 장점이 있다. Evidence theory는 probability와 interval 방법의 중간에 해당하며, 구간별 probability assignment를 세분화 할수록 probability결과에 접근한다. 이 방법은 계산비용이 가장 높은 것이 문제이다.

The goal of this research was to develop a portable system that could be used to evaluate the quality of milk in real time at a raw milk production site. A real-time portable quality evaluation system for raw milk was developed to enable non-destructive quality evaluation of somatic cell count (SCC), fat, protein, lactose, and total solid (TS) in milk samples. A prediction model of SCC, fat, protein, lactose, and TS was constructed using partial least squares (PLS) and 200 milk samples were used to evaluate the prediction performance of the portable quality evaluation system and high performance spectroscopy. Through prediction model development and verification, it was found that the accuracy of high performance spectroscopy was 90% for SSC, 96% for fat, 96% for protein, 91% for lactose, and 97% for TS. In comparison, the accuracy of the portable quality evaluation system was relatively low, at 90% for SSC, 95% for fat, 92% for protein, 89% for lactose, 92% for TS. However, the measurement time for high performance spectroscopy was 10 minutes for 1 sample, while for the portable quality evaluation system it was 6 minutes. This means that the high performance spectroscopy system can measure 48 samples per day (8 hours), while the portable quality evaluation system can measure 80 (8 hours). Therefore, it was found that the portable qual ity evaluation system enables quick on-site quality evaluation of milk samples.

모델기반 고장 진단법은 풍력발전기의 상태감지 시스템(Condition monitoring system)에 적용을 목적으로 변동 하중조건하에서 작동하는 기어박스내의 기어의 균열을 진단하기 위해 제시하였다. 두 개의 평기어(Spur gear)로 구성된 간단한 테스트 베드가 위의 접근방법을 검증하기 위해 구축되었고, 기어의 균열은 기어의 뿌리부분에 균열을 인가하여 묘사하였다. 축의 회전속도에 독립적인 타코미터를 기반으로 한 오더분석(Order analysis)을 균열크기 진단에 적용하였고, 테스트 베드의 작동을 시뮬레이션하기 위해 집중변수 모델(Lumped parameter dynamic model)이 사용되었다. 모델에서 균열과 밀접히 관련된 변수는 측정된 신호와 시뮬레이션된 신호 간의 차이를 최소화하는 최적화 기법으로 역추정하였다. 제시한 방법의 유효성을 보이기 위해, 미리 정의된 모델 변수로부터 생성된 시뮬레이션 신호를 테스트-베드로부터 측정된 신호로 가정하고, 제시한 방법을 사용하여 변수를 역추정하였다. 결과는 실제 값과 일치하였고, 이를 통해 알고리즘이 제대로 작동함을 알 수 있었다. 다음 연구에서는 실제 테스트 베드의 실제균열에 적용하고자 한다.

This study examines the effects of different environments on the application of hemiplegia patients circuit balance training. Group 1 performed circuit balance training without any auditory intervention Group 2 performed training in noiseless environments and Group 3 performed training in white noise environments. First, among lower extremity muscular strength evaluation items, maximum activity time(MAT) was not significantly different(p>.05). Maximum muscle strength(MMS) increased significantly in Group 3(p<.01), there was no significant difference in MMS among the groups. Average muscle strength(AMS) indexes also significantly increased in Group 3(p<.01), there was no significant difference in AMS among the groups. Second, among balancing ability evaluation items, Berg's balance scale(BBS) scores significantly increased in all groups(p<.05), BBS scores were significantly difference among the groups. Based on the results, Group 1, 2 and Group 1, 3 showed significant increases (p<.05). Functional reach test(FRT) values significantly increased in Group 2, 3(p<.05), and there was no significant difference in FRT values among the groups. Timed up and go(TUG) test values significantly decreased in Group 2, 3(p<.05), and there was no significant difference in TUG test values among the groups. Third, among walking speed evaluation items, the time required to walk 10m significantly decreased in all groups(p<.05), and there was no significant difference in the values among the groups. Average walking speeds showed significant increases in Group 1, 3(p<.05), and there was no significant difference in the values among the groups. Based on the results of this study, noise environments should be improved by either considering auditory interventions and noiseless environments, or by ensuring that white noise environments facilitate the enhancement of balancing ability.

In order to select the superior lines of bumblebee queens(Bombus terrestris) in oviposition and colony development which has some characteristics of diapause with adult type, undiapaused bumblebee queens of 3rd generation were surveyed in room condition compared to queen treated with CO2 and artificial hibernation. Oviposition rate and preovipositional days of undiapaused queens was lower in efficacy with 46.7% and shows same trend in preovipositional days with 52.8 days than the other treatment with CO2 anesthesia and artificial hibernation with 60.0%, 86.7%, 26.1 days and 26.6 days respectively. In continuous experiment, we also surveyed ovipositional characteristic and colony development of three types of queens emergenced from undiapaused queens and treated queens with CO2 and artificial hibernation. Although undiapaused queens in 1st generation were more effective in the same condition than treated queens, but the undiapaused queens of 3rd generation emergenced from undiapaused queens of 2nd generation were very low trend in all checkpoints compared with treated queens in same generation. This results was shown that the queens treated with CO2 or artificial hibernation need to be in successive mass rearing continuously in the year round.

The purpose of current study is to investigate the beneficial effect of enzyme (Alcalase) hydrolysates of silk protein in rat. Alcalase-treated silk protein hydrolysate (ATSH) itself did not show any cytotoxicity on the hepatic tissues and blood biochemistry, similar to the normal condition. ATSH played a protective role in tert-butyl hydroperoxide (t-BHP)-induced hepatotoxicity and liver damage. The values of AST (aspartate aminotransferase) and ALT (alanine aminotransferase), which are the indicators of the liver function, were effectively alleviated with the ATSH treatment in a dose dependent manner. The level of Lactate dehydrogenase (LDH) and Malondialdehyde (MDA), which were increased with t-BHP treatment, were significantly reduced by ATSH. High dose of ATSH (2 g/kg) reduced the t-BHP-induced LDH release by 48%. Antioxidant and antioxidant enzymes in liver cells were significantly increased by ATSH treatment in their level and activities. ATSH (2 g/kg) increased glutathione (GSH), an intracelluar antioxidant, by 2.5-fold compared with the t-BHP treated group. The activities of glutathione-s-transferase (GST), superoxide dismutase (SOD), and catalase were also elevated by 38%, 60%, and 45%, respectively, with ATSH (2 g/kg) treatment. The antioxidative effect of ATSH was recapitulated to the protection from t-BHP induced liver damages in hematoxylin and eosin (H&E) staining. Thus, ATSH might be used as a hepatoprotective agent.

Omija-cheong, concentrated extracts from sugar-treated Omija fruit (Schisandra chinensis Baillon), is produced by traditional manner in Korea. The quality characteristics of Omija-cheong processed at low temperature with a pilot-scale were investigated to optimize the incubation time. With increasing incubation time in processing Omija-cheong, the pH level of Omija-cheong remained constant, while titratable acidity and organic acids increased. Fresh Omija fruits contained citric, malic and succinic acids, most of which were extracted into concentrated extracts after 37 days of incubation and reached to the stable concentration after 47 days of incubation. Titratable acidity in Omija-cheong gradually increased from 1.18% to 2.71%, and also was correlated with total concentration of organic acids. About 80% of supplemented sucrose for manufacturing Omija-cheong was converted into glucose and fructose until 68 days of incubation, and the composition of free sugars was maintained to be stable up to 138 days of incubation. The contents of total flavonoids and phenolic compounds in Omija-cheong were 24.1 mg-GAE/L and 1,635 mg-QE/L at 57 days of incubation, which were more than 9 and 5 times higher than those in Omija fruits, respectively. From the quality characteristics in processing Omija-cheong by low-temperature incubation, more than 60 days of incubation is required for the constant quality and value-added beverage.