The present study analyzes the role of Lactic Acid Bacteria Mixture (LBM) on improving rye silage quality. Rye of four different stages (Booting, Heading, Flowering, and Late flowering) was collected and silage was prepared. The nutrient profile analysis of experimental silage groups showed no significant changes between control and LBM inoculation. Interestingly, the pH of rye silage in LBM treatments showed significant reduction than control (p<0.05) in all stages of rye silage. However, lowest pH (3.69) resulted on booting stage among other stages of rye. Subsequently significant lactic acid production was noted in all stages of LBM inoculation than control. Conversely maximum lactic acid production of (5.33%DM) was noted at booting stage followed by (4.86%DM) in heading stage. Further the lactic acid bacterial (LAB) count in LBM inoculated group showed significant increase than control. Similarly, the silage of booting stage group registered maximum LAB population (63.7 ×106CFU/g) after that heading stage (32.3 ×106CFU/g). Further significant reduction in yeast growth and no fungal growth was noted in all LPM treatment groups. Hence, LBM inoculants could be a better additive for improving rye silage quality.

This study was conducted to find out the effects of over seeding using new grass varieties orchardgrass ‘Kordione’ and ‘Onnuri’, and tall fescue ‘Greenmaster’ on botanical composition and dry matter productivity from 2013 to 2016 in low productivity hilly pasture, middle area of Korea. There were used 3 grass seed mixture types made of different compositions and amounts {T1: control - no over seeding, T2: tall fescue (TF) ‘Greenmaster’ 18 kg ha-1, orchardgrass (OG) ‘Kordione’ 9 kg ha-1, Perennial ryegrass (PRG) ‘Linn’ 5 kg ha-1, and Kentucky bluegrass (KBG) ‘Kenblue’ 2 kg ha-1, T3: TF ‘Greenmaster’ 9 kg ha-1, OG ‘Kordione’ 18 kg ha-1, PRG ‘Linn’ 5 kg ha-1, and KBG ‘ Kenblue’ 2 kg ha-1, T4: TF ‘Greenmaster’ 9 kg ha-1, OG ‘Onnuri’ 18 kg ha-1, PRG ‘Linn’ 5 kg ha-1, and KBG ‘Kenblue’ 2 kg ha-1}. In the botanical composition of grassland, T1 nearly remained constant in other seasons while ratio of weeds were increased in summer season. T2 was better than control (T1) in portion of grass ratio, which has increased by 80%. In the early time of establishment, the portions of OG were increased in T2, but TF rate was increased after 3 years later when grass was established. T3 and T4 showed a very similar patterns, grass ratio had increased by 80% and the portion of KBG had increased as time passed. T2 (129,763 kg ha-1) was showed the highest dry matter yield than other treatments (T1: 6,756 kg ha-1, T3: 9641, and T4: 10,738) in 2016.

This study evaluated the effect 3 types of conditioning (Untreated, Impeller, Roller) and the number of times for tedding (rake-teder) performed in one day on the drying rate of tall fescure hay. The experiment was performed at The forage site of Cheonan-si seongwhan-eup National Institute of Animal Sciencee. It took 3 days to make tall fescue hay. The Impeller and Roller conditioning types shortened the haymaking period by 1 day compared to untreated hay. Impeller and roller samples had less than 20% of water content by the 23rd-day at 13 o’clock. Tedding frequency failed to affect haymaking. There was no correlation between the three types of conditioning or tedding frequency and acid digestible fiber (ADF), neutral detergent fiber (NDF), total digestible nutrients (TDF), crude protein (CP), or relative feed value (RFV) in tall fescue hay. CP was 12.5~12.9% for the three types of conditioner with 1 or 2 times of tedding a day, Threrfore, conditioner types and gedding frequency had no significant effect on CP.

This study was conducted to determine the effect of rainfall after cutting on nutritive value of Italian ryegrass (Lolium multiflorum) hay. Fresh Italian ryegrass was treated with 3 days natural rainfall (56.5, 14.0 and 1.6 mm) after cutting without tedding. Rainfall effect on Italian ryegrass quality was evaluated based on Neutral detergent fiber (NDF) and acid detergent fiber (ADF), crude protein (CP), crude fat (CF), crude ash (CA) and non-fiber carbohydrate (NFC). The quality of Italian ryegrass was decreased with extending raining days. The content of neutral detergent fiber (NDF) was increased from 0.4 to 4.7% by 1 day advancement in raining. CF content was decreased from 8.1 to 32.8%. CP and CA contents did not change, but NFC content was decreased from 0.5 to 8.4%. The quality damage by the number of raining days after cutting Italian ryegrass was the lowest on the first day from exposure to rain.

Nitrogen (N) fertilizer management is one of the important aspects of economic production of sorghums in sustainable agriculture. The aim of the study was to evaluate the effects of different N application rates and its split N application methods on productivity, growth characteristics, N accumulation, N use efficiency (NUE), and feed value of Sorghum × Sudangrass hybrids. Treatments consisted of five N application rates (0, 150, 200, 250, and 300 kg ha-1) and two split N application methods (40% in basal N, 30% at the growing stage, and 30% after the first harvest vs. 50% in basal N and 50% after the first harvest). Plant height, leaf width, and stem diameter were increased (p≤0.05) with increasing N fertility rates at each harvest. Chlorophyll content (expressed as SPAD values) was the highest at a rate of 300 kg N ha 1 (first harvest, 46.32; second harvest, 33.09). It was the lowest at zero N (first harvest, 21.56; second harvest, 18.5). Total N, N uptake, and NUE were increased with higher N rates. Split N application had little effect on total N, amount of N uptake, or NUE. Total dry matter yields were the highest (21,715 kg ha-1) at a rate of 300 kg N ha-1. It was the lowest (10,054 kg ha-1) at zero N. Our results suggest that more than 300 kg N ha-1 can improve dry matter yield to be above 116% compared to zero N, thus enhancing the agronomic characters of sorghums. However, no significant effect had been found for split N application. Further work is needed to determine the optimal N levels and the effect of split N application rates.

In order to provide basic data to predict changes of milk components in milk yield, this study was conducted to compare nutritive values of diets collected from organic dairy farms (ODF) and conventional dairy farms (CDF) of Chungnam and Jeonbuk in Korea. The contents of crude fat, crude fiver, neutral detergent fiber, and acid detergent fiber in diets of ODF were significantly (p<0.05) higher compared to those of CDF. The contents of crude protein and crude ash in ODF were also higher (p<0.05) than those of CDF. The contents of calcium, ferrum, and phosphorus in diets of ODF were similar to those of CDF. The contents of potassium, magnesium, and iodine in diets of ODF were higher (p<0.05) than those of CDF. The contents of total saturated and unsaturated fatty acids in diets of ODF were higher (p<0.05) compared to those of CDF. The contents of oleic acid, linoleic acid, and linolenic acid in diets of ODF were higher (p<0.05) compared to those of CDF. However, the contents of myristic acid, palmitic acid, and stearic acid in diets of ODF were lower (p<0.05) compared to those of CDF.

The present study was carried out to explore the potential effects of novel lactic acid bacteria Pediococcus penticeous KCC-23 (KCC-23) and Lactobacillus plantarum KCC-24 (KCC-24) on rye haylage fermentation at National Institute of Animal Science, Cheonan province in Korea. The experiment contains three different groups such as control without lactic acid bacteria, rye haylage with KCC-23 and rye haylage KCC-24. After experimental periods, the content of crude protein, acid detergent fiber (ADF), neutral detergent fiber (NDF), the activity of total digestible nutrient (TDN) and In-vitro digestibility (IVD) was similar in control and LAB treated haylage (p<0.05). The pH was reduced in KCC-23 and KCC-24 treated rye haylage as compared to control (p<0.05). The lactic acid concentration in haylages was increased by L. plantarum KCC-23, and KCC-24 supplement. Whereas, the less amount of acetic acid and butyric acid was noted in KCC-23 and KCC-24 treated haylage as compared with control (p<0.05). The KCC-23 and KCC-24 were dominantly grown in experimental haylage as compared with control. It indicates, the addition of KCC-23, and KCC-24 enhances fermentation quality of haylages as compared control. The present study suggests that KCC-23 and KCC-24 are potent strains that were improving the fermentation process in rye haylage

본 연구에서는 호밀을 이용하여 사일리지 제조시 Lactobacillus plantarum KCC-10 및 KCC-19를 접종하여 사일리지의 사 료가치, 품질 및 미생물상의 변화를 조사하였다. 호밀 사일 리지에서 젖산균 접종구와 무접종구에서 사료가치는 크게 변화되지 않았다. 그리고 유산 함량은 젖산균 접종구가 현 저하게 높은 경향을 보였으나 초산과 낙산은 젖산균 접종 구가 약간 낮아지는 경향을 보였다. 젖산균 분포는 젖산균 접종구에서 현저하게 높았으며 효모와 곰팡이는 104CFU/g 에서 관찰되지 않았다. 호밀 사일리지 제조시 KCC-10 및 KCC-19 접종구의 조단백질 및 TDN 함량은 무접종구 보 다 약간 증가하였으나 섬유소 및 in vitro 건물소화율에서 는 비슷한 수준을 보였다. 이상의 결과를 요약해 보면 호 밀 사일리지 제조시 KCC-10 및 KCC-19 접종에 의해 사일 리지의 품질이 향상되었다.

Three growing wethers were used to investigate the differences in nitrogen (N) retention, blood plasma metabolite concentration and energy-yielding nutrient supply to muscle and adipose tissue. The wethers were fed one of three diets: timothy hay with concentrate (THD), Italian ryegrass with concentrate (IRD), and rice straw with concentrate (RSD) for 11 days. The experimental diets were adjusted to the animals to provide 100 g of daily gain. The triglyceride (TG) concentration of blood plasma in arterial and portal veins was higher with THD and IRD than with RSD. Conversely, the available amount of TG in tissues was higher with IRD. The daily amount of glucose and non-esterified fatty acids (NEFA) supplied to muscle tissue and adipose tissue was numerically higher with THD than IRD or RSD. Although N retention did not differ among the diets, it was numerically higher with THD than with IRD or RSD. The results suggest that the difference in the amount of glucose and NEFA delivered to muscle tissue may reflect the N retention in response to forage based diets.

The objective of the study was to measure the beneficial effects of lactic acid bacteria (LAB) inoculation on the nutritive value of oat silage collected from thirteen regions in the Republic of Korea. The contents of crude protein, acid detergent fiber (ADF), neutral detergent fiber (NDF) and crude ash (CA) were slightly lower in LAB inoculated silage when compared with the control silage, whereas inoculation of LAB resulted in increased total digestible nutrient (TDN). Higher number of LAB, but lower count of yeast and fungi indicated the effectiveness of the LAB inoculation on oat silage fermentation. LAB inoculation resulted in low pH silage, which may prevent undesirable microbial growth. The LAB inoculation promoted lactic acid dominant fermentation with marginal levels of acetic acid and butyric acid in oat silage. These data suggest that the LAB inoculation may preserve oat silage at better quality for ruminant animal production.

An experiment was carried out to determine the homofermentative activity of Lactobacillus plantarum KCC-10 and KCC-19 on the ensiling of whole crop barley (WCB). The crude protein in the silages was slightly higher in the KCC-10 and KCC-19 treatments compared to the control, but there was no significant difference between the two inoculant-treated silages. Nutrient parameters such as acid detergent fiber, neutral detergent fiber and in vitro dry matter digestibility in L. plantarum KCC-10 and KCC-19 treated silages did not differ from those in the control silage. The lactic acid content increased in KCC-10 and KCC-19 treated silage when compared with the control silage but the contents of acetic acid and butyric acid produced in KCC-10 and KCC-19 treated silages were similar with the control silage. Further, the number of lactic acid bacteria (LAB) in KCC-10 treated silage demonstrated a significant increase when compared to the control. Especially, KCC-19 treated silage showed greater lactic acid bacterial growth potential. Other microbes such as yeast and fungi were not detected in KCC-10 and KCC-19 treated WCB silages. Hence, this study suggests that the addition of L. Plantarum KCC-10 and KCC-19 to the WCB silage can improve fermentation quality for the production of high-quality silage.