왕피천 수계 내 저서성 대형무척추동물의 군집구조 분석을 위해 총 5개 지점에서 2023년 총 4회(4월, 6월, 8월 11월) 조사를 실시하였다. 조사기간 중 저서성 대형무척추동물은 총 5문 7강 17목 77과 156종 17,179.1개체/㎡가 채집되었다. 수환경 변화에 민감한 E.P.T. 분류군은 전체 156종 중 91종이 출현하여, 전체 출현종의 58.3%를 차지 하였다. 섭식기능군(FFGs) 분석결과, 종 출현 양상은 육식성 포식자(Predator: P)가 51종(32.69%)으로, 개체 출현 양상은 주워먹는 무리(Gathering-collector: GC)가 6,867.2개체/㎡(39.97%)로 높은 비율로 출현하였다. 서식기능 군(FHGs) 분석결과, 붙는 무리(Clinger: CL)가 70종(44.87%), 12,720.6개체/㎡(74.04%)로 가장 높은 비율로 출현 하였다. 군집지수 분석결과, 우점도지수(DI) 0.43, 다양도지수(H′) 3.51, 풍부도지수(R1) 4.59 균등도지수(J′) 0.77 로 나타났다. 생물학적 수질 판정 지수(BMI) 분석결과, 평균 92.36(±0.83)으로 모든 지점에서 “매우 좋음”으로 판정되었다.

곤충병원성 선충인 Heterorhabditis. megidis(큰형광병원선충)는 참나무 군락지(광주광역시 소재) 사양토에 서 분리 동정되었고, 곤충병원성을 가지는 공생세균은 Photorhabdus temperata로 분리 동정되어 각각 GJ1-2로 명명하였다. H. megidis GJ1-2는 고구마(주황미) 큰 검정풍뎅이 Holotrichia parallela에 대한 방제 효과는 평택시 (경기도 소재)와 논산시(충청북도 소재)의 노지 재배 사양토에서 관주처리 30일 후 각가 81.1%, 78.9%로 나타났 으며, 인삼(재래종) 거세미나방 Agrotis segetum에 대한 방제 효과는 이천시(경기도 소재)와 청주시(충청북도 소재)의 비가림 재배(차광막 설치) 사양토에서 관주처리 14일 후 방제가는 각각 79.9%, 80,2%로 나타났다. H. megidis GJ1-2는 고구마(주황미)와 인삼(재래종)에서 약제 처리 후 3, 7, 14일 후 외관상 약해는 나타나지 않았다.

The blackish cicada, Cryptotympana atrata Fabricius, 1775 (Hemiptera: Cicadidae) was originally distributed mainly in the southernmost remote island, Jeju and rarely throughout low lands in South Korea, but has been explosively increased at the urban areas, where annual temperature is higher. In this study, we sequenced a partial mitochondrial COI from a total of 171 individuals collected throughout 12 localities in South Korea. The haplotype found with the highest frequency in Jeju island shares only with two inland localities in southern region with a low frequency, whereas the haplotype found with the highest frequency throughout inland localities was not found in Jeju island. These results showed that Jeju population, southern region, and other inland populations form somewhat different genetic groups.

국내 농작물에 크게 피해를 주고 있는 파밤나방, 담배거세미나방과 담배나방에 대하여 2020년부터 4년 동안 7월과 9월에 전국 7개 지역의 발생 추이를 조사하였다. 조사지역은 경기도 안성, 충청북도 청주, 강원도 횡성, 경상북도 김천과 안동, 경상남도 창녕, 전라북도 김제를 포함하고 있다. 각 해충의 포획 마릿수는 2021년과 2022 년도에 크게 다르지 않았으며, 지역 간 해충 발생 수준도 비슷하였다. 2021년도와 2022년도와 비교해서 2023년도 에 담배거세미나방과 파밤나방은 각각 119% 및 70% 증가했지만, 담배나방은 23% 감소하였다. 전체적으로 지역 별 발생 시기 및 발생량이 매년 차이를 보이는 것은 기후 등 환경변화의 영향이 가장 큰 것으로 사료 된다. 전체 해충 포획수는 파밤나방이 가장 많고, 담배나방의 수가 가장 적었다. 전년 대비 담배거세미나방과 파밤나방의 경우 중부지역이 남부에 비해 더 많이 발생하였다. 9월에 더 많이 포획되어 전년과 유사한 경향을 보였으며, 최근 3년 동안 9월 발생 밀도가 가장 높았다. 담배나방은 전년에 비해 발생량이 급감하였으며, 안성지역을 제외한 대부분 지역에서 감소하였다.

2023년 경상북도 상주시 떫은감 과수원에서 잎 표피아래 굴을 파고 들어가면서 원형 또는 부정형의 식흔자국 을 남기는 증상을 관찰하였다. 해충의 유충은 녹색에서 적색으로 변하며 번데기 단계를 거쳐 성충(길이 3㎜, 갈색) 이 되어 탈출하였다. 본 해충에 대해 잎에서의 피해증상과 실험실에서 유전분석 결과 Genbank(GU816671,486bp DNA liner, 2016)에 등재되어 있는 감잎가는나방(Cuphodes diospyrosella)으로 동정되었다. 일본에서 1957년 최초 보고 후 우리나라에서는 주로 경남 창원과 진주의 농약 무살포 및 유기농 단감원에서 발생하였으나, 이번 경북지 역에 처음 발견되어 떫은감 재배 농업인과 현장지도원의 감잎가는나방에 대한 피해증상 진단에 활용할 수 있을 것으로 생각된다.

The large copper butterfly Lycaena dispar (Haworth, 1803; Lepidoptera: Lycaenidae) has been categorized as a near-threatened species (NT) in South Korea from 2012 mainly due to limited distribution. In this study, we visited 36 sites spread across all South Korean provinces to verify the distributional range of the species and sequenced mitochondrial COI for 53 individuals from nine sites. We observed L. dispar at 15 sites in six provinces, including the two previously known provinces, indicating a southward range expansion. The in-field monitoring and genetic data collectively suggested that L. dispar does not have a limited distribution nor is it isolated, indicating that it should be reclassified as less vulnerable. Our study demonstrates that the combination of field and genetic data can provide a more reliable assessment of the stability of a species.

The recent increase in the occurrence of common bed bug and tropical bed bug in shared areas highlights the need for rapid species identification at infestation sites, which is crucial for implementing targeted control measures due to differences in genetic and physiological traits. In this study, molecular diagnostic methods were developed using species-specific ITS2 sequences. Both multiplex PCR and loop-mediated isothermal amplification (LAMP) protocols with a DNA release method successfully distinguished between the two bed bug species regardless of developmental stages in 0.5~2.5 hours, even with dead specimens. Especially, LAMP's simplicity and speed make it applicable for rapid and accurate bed bug diagnosis at infestation sites.

본 연구는 대한민국 울릉도 특산식물인 추산쑥부쟁이(Aster × chusanensis Y.S.Lim, Hyun, Y.D.Kim & H.C.Shin)의 기내 대량증식체계 확립을 위해 시토키닌 계열 식물생장조절 제를 첨가하여 캘러스 및 신초 형성 조건을 구명하고자 수행 하였다. 실험결과, 추산쑥부쟁이의 줄기 절편체에서 캘러스 와 신초가 형성되었다. 캘러스 형성률은 TDZ 3.0mg·L-1처 리구에서 88.9%로 가장 높았고 BAP 3.0mg·L-1와 Zeatin 3.0mg·L-1 처리구는 66.7%, 2iP 3.0mg·L-1 처리구에서는 11.1%였다. 신초 형성률은 모든 처리구에서 77% 이상이고 특히 TDZ처리구와 Zeatin 처리구에서 100%였다. 그러나 TDZ처리구에서는 형성된 신초가 부풀어 오르거나, 잎에 과수 성이 나타나는 등 형태적 기형이 나타났다. Zeatin처리구에 서 발생한 신초는 정상적인 형태를 나타났다. 잎 절편체에서 는 캘러스, 신초가 발생하지 않았으며 절편체가 탈색하며 고 사하는 것을 확인하였다. 종합적으로 추산쑥부쟁이의 캘러 스 및 신초 형성을 위해서는 줄기 절편체를 배양 재료로 이 용하는 것이 효과적으로 확인되었다. 캘러스 유도에는 TDZ 3.0mg·L-1처리가 가장 효과적이었고, 추후 NAA와 같은 옥신 을 함께 첨가하여 증식 효과를 분석하는 연구가 필요할 것이 다. 또한, 신초 유도에도 TDZ 3.0mg·L-1 처리가 효과적이었 으나 장기간, 고농도로 사용할 경우 형태적인 기형이 발생할 수 있어 정상적인 식물생산에 부적합한 것으로 확인되었다. 따라서 형태적 기형이 발생하지 않은 아데닌 유도체형 시토키 닌인 Zeatin을 활용하는 것을 추천한다. 본 연구는 울릉도 특 산식물인 추산쑥부쟁이의 기내 대량증식과 생태계 유지에 필 요한 종 공급을 위한 대량증식 체계 구축을 위한 자료로 활용 할 수 있을 것이다.

Pasture formation and management are crucial to avoid yield reduction. This experiment aimed to examine the effects of tall fescue-centered mixed-seeding combinations on yield and vegetation changes in perennial pastures in the central region for two years, from September 2020 to October 2022. The treatments were arranged in three replications in a randomized block design: control (C), tall fescue-based mixture-1 (T-1), and tall fescue-based mixture-2 (T-2). The tall fescue (TF), orchard grass (OG), perennial ryegrass (PRG), Kentucky bluegrass (KBG), and white clover (WC) were used. The emergency rate of grasses (70.0 to 73.3%) did not differ among mixed seeding combinations. Overwintering rates (81.7 to 83.3%) were similar among treatments. The plant height of grasses was similar at each harvest date, with the highest height (86.2 cm) recorded in the second harvest of the first year, followed by that (58.4 cm) in the third harvest of the first year; it was least (38.9 cm) in the fourth harvest of the second year. There was no significant difference in the dry matter yield of grasses among the mixed seeding combination treatments in the first, third, or fourth harvests of the first year (p>0.05). For second-year grasses, dry matter yield was not significantly different in harvest date among the treatments (p>0.05). Based on mixed seeding ratio, orchard grass showed the highest yield at 70% in the C treatment, followed by tall fescue at 80% and 60% in the T-1 and T-2 treatments, respectively, in the first harvest after seeding. There was no significant difference in feed value between treatments (p>0.05), but a significant difference was observed between the third and fourth harvest (p<0.05). Therefore, it indicated that it is important to create perennial pastures in the central region through mixed seeding combinations centered on tall fescue.

Heavy water (deuterium oxide, D2O) is water in which hydrogen atoms (1H, H), one of the constituent elements of water molecules, have been replaced with deuterium (2H, D), a heavier isotope. Heavy water is used in a variety of industries, including semiconductors, nuclear magnetic resonance, infrared spectroscopy, neutron deceleration, neutrino detection, metabolic rate studies, neutron capture therapy, and the production of radioactive materials such as plutonium and tritium. In particular, heavy water is used as a neutron moderator or coolant in nuclear reactors and as a fuel for nuclear fusion energy, methods for measuring heavy water are becoming increasingly important. There are methods with density, mass spectrometry, and infrared (IR) spectroscopy. In this study, Fourier transform infrared spectroscopy (FT-IR) was used, which is commonly used in IR spectroscopy because of its relatively high analytical sensitivity, low operating costs, and easy online analysis. Heavy water was identified in the range of 2,300-2,600 cm-1 wavenumber (O-D) and the range of 1,200-1,300 cm-1 wavenumber (D-O-D), which are known to be the range with strong infrared absorption. As a result, the linearity of infrared absorbance for each heavy water concentration was confirmed within the relative expansion uncertainty (k=2).

When occurring at a nuclear power plant (NPP) by accidents, accurate prediction and identification of the process of radioactive material dispersing into atmosphere is important to protect public and environment. Atmosphere dispersion of radioactive materials is significantly influenced by wind direction and wind speed. The government and nuclear operator continuously monitor wind data at nuclear sites through meteorological tower to prepare for such accidents involving the release of radioactive materials. The purpose of this study is to construct wind rose diagrams at 5 NPP sites (Kori, Saewool, Wolsong, Hanbit, Hanul). Wind roses serve as invaluable tool for identifying wind patterns in each region and visualizing wind directions. This can be utilized to predict the dispersion pathway and extent range of radioactive materials carried by the wind. This program will take on the role of establishing appropriate evacuation routes or shelter locations for residents when reliable wind data is not immediately available during an NPP accident. The wind data used in the study was collected from a meteorological tower located at the NPP site, and measurements were taken at 1-hour intervals for each operation over a period of ten years. The collected data underwent preprocessing, followed by the development of Python code to render the wind rose diagrams in an interpretable format. The future direction of this study will be focused on enhancing this program by integrating geographical mapping capabilities. With these advancements, it will become feasible to superimpose shelter positions on a map in accordance with prevailing wind directions. These improvements will contribute to the development of additional protective measures for residents and the proposal of alternative shelter options in response to potential radioactive material releases.

The mobility of radionuclides in the subsurface environment is governed by a interaction of radioactivity characteristics and geochemical conditions with adsorption reactions playing a critical role. This study investigates the characteristics and mechanisms of radionuclides adsorption on site media in viewpoint of nuclear safety, particularly focusing on the potential effect of seawater infiltration in coastal site near nuclear power plant. Seawater intrusion alters the chemistry in groundwater, including parameters such as pH, redox potential, and ionic strength, thereby affecting the behavior of radionuclides. To assess the safety of site near nuclear power plant and the environmental implications of nuclide leakage, this research conducted various experiments to evaluate the behavior of radionuclides in the subsurface environment. High distribution coefficients (50-2,500 ml/g) were observed at 10 mg/L Co, with montmorillonite > hydrobiotite > illite > kaolinite. It decreased with competing cations (Ca2+) and was found to decrease significantly by 90% with a decrease in pH to 4. It is believed that the adsorption capacity of cationic radionuclides decreases significantly as the clay mineral surface becomes less negatively charged. For Cs, the distribution coefficient (180-560 ml/g) was higher for montmorillonite > hydrobiotite > illite > kaolinite. Compared to Co, it was found to be less influenced by pH and more influenced by competing cations. For Sr, the distribution coefficient (100-380 ml/g) was higher in the order of hydrobiotite > montmorillonite > illite > kaolinite. Compared to Cs, it was found to be less affected by pH and also less affected by the effect of competing cations compared to Cs. Seawater samples from Gampo and Uljin site near Nuclear Power Plant in Korea were analyzed to determine their chemical composition, which was subsequently used in adsorption experiments. Additionally, the seawater-infiltrated groundwater was synthesized in laboratory according to previous literature. The study focused on the adsorption and behavior of three key radionuclides such as cesium, strontium, and cobalt onto four low permeability media (clay minerals) such as kaolinite, illite, hydrobiotite, and montmorillonite known for their high adsorption capacity at a site of nuclear power plant. At concentrations of 5 and 10 mg/L, the adsorption coefficients followed the order of cobalt > cesium > strontium for each radionuclide. Notably, the distribution coefficient (Kd) values exhibited higher values in seawater-infiltrated groundwater environments compared to seawater with relatively high ionic strength. Cobalt exhibited a substantial adsorption coefficient, with a marked decrease in Kd values in seawater conditions due to elevated ionic strength. In contrast, cesium displayed less dependency on seawater compared to other radionuclides, suggesting distinct adsorption mechanisms, possibly involving fractured edge sites (FES) in clay. Strontium exhibited a significant reduction in adsorption in seawater compared to groundwater in all Kd sorption experiments. The adsorption data of cobalt, cesium, and strontium on clay minerals in contact with seawater and seawater-infiltrated solutions offer valuable insights for assessing radioactive contamination of groundwater beneath coastal site near nuclear power plant sites. This research provides a foundation for enhancing the safety assessment protocols of nuclear power plant sites, considering the potential effects of seawater infiltration on radionuclide behavior in the subsurface environment.

EU taxonomy requires to solve problems for safe management of radioactive waste and disposal of spent fuel, which is a precondition for growing demand for nuclear power plant. Currently, Korea manages about 18,000 tons of high-level radioactive waste at temporary storage facilities in nuclear power plant sites, but such temporary storage facilities are expected to become saturated sequentially from 2031. Therefore, it is necessary to secure a permanent disposal facility to safely treat high-level radioactive waste. In accordance with the second basic plan for high-level radioactive waste management in 2021, it is necessary to establish requirements for regulatory compliance for the site selection and site acquisition, investigation and evaluation, and construction for the establishment of a deep geological disposal facility. In this study, we analyzed the regulatory policies and cases of leading foreign countries related to deep geological disposal facilities for high-level radioactive waste disposal waste such as IAEA, USA, Sweden, and Finland using data analysis methodology. To analyze a large amount of textbased document data, text mining is applied as a major technology and a verification standard that secures validity and safety based on the regulatory laws described so far is developed to establish a regulatory base suitable for domestic deep geological disposal status. Based on the collected data, preprocessing and analysis with Python were performed. Keywords and their frequency were extracted from the data through keyword analysis. Through the measured frequency values, the contents of the objects and elements to be regulated in the statutory items were grasped. And through the frequency values of words co-occurring among different sections through the analysis of related words, the association was obtained, and the overall interpretation of the data was performed. The results of analyzing regulations of major foreign countries using text mining are visualized in charts and graphs. Word cloud can intuitively grasp the contents by extracting the main keywords of the contents of the regulations. Through the network connection graph, the relationship between related words can be visually structured to interpret data and identify the causal relationship between words. Based on the result data, it is possible to compare and analyze the factors to be supplemented by analyzing domestic nuclear safety case and regulations.

곤충병원성 선충인 Heterorhabditis. megidis(큰형광병원선충)는 참나무 군락지(광주광역시 소재) 사양토에 서 분리 동정되었고, 곤충병원성을 가지는 공생세균은 Photorhabdus temperata로 분리 동정되어 각각 GJ1-2로 명명하였다. H. megidis GJ1-2는 고추(녹광), 애호박(농우), 오이(백다다기), 참외(금싸라기), 토마토(베타티니) 유묘기 경엽처리와 배추(씨알천하일품), 상추(진청맛), 완두(청진주), 수박(삼복꿀), 무(서호) 유묘기 관주처리 에서 21일차까지 기준량과 배량에 대하여 약해(약해 판정기준결과: 0)가 나타나지 않았다, H. megidis GJ1-2는 배추 파밤나방에 대한 방제 효과는 이천시(경기도 소재)와 수원시(경기도 소재)의 시설재배 포장에서 각각 경엽 처리 3일차 71.8%. 73.9%, 7일차 86.2%, 84.6%로 나타났으며, 옥수수 열대거세미나방에 대한 방제 효과는 제주시 (제주특별자치도 소재)의 노지(미사질 양토)에서 경엽처리 7일차 82.6%로 나타났다. 그리고 H. megidis GJ1-2는 랫드를 이용한 급성경구와 급성경피 독성시험 반수치사농도(LD50) 값은 2,000 mg/kg B.W 초과로 IV급(저독성) 으로 구분되었고, 토끼를 이용한 피부와 안점막에 자극성이 없는 것으로 구분되었다. 그리고 담수어류(송사리) 에 대한 급성독성시험 반수치사농도(LD50) 값은 영향을 미치지 않는 기초시험농도 100 mg/L를 초과하였다.

This study aimed to develop a new eco-friendly control method of B. tabaci using a trap plant treated with systemic insecticide. Due to high preference, tobacco plant was selected as a trap plant among solanaceous plants. Out of all 6 systemic insecticides, dinotefuran WG showed the highest mortality (> 97.3 %) against B. tabaci. The concentrations of dinotefuran in tobacco leaves after systemic treatment showed the sharp increase in the pesticide from 2 hrs until 48 hrs (2 d) and steady increase over 35 d. The mortality of B. tabaci in tobacco leaves was about 80% at 9 hrs after treatment and over 90% at 1, 2, 3, 6, 9, and 35 d after treatment. The number of B. tabaci was counted every week after dinotefuran-treated tobacco pots were placed in the tomato greenhouse cultivation. The density of the insects with 3 tobacco plants was app. 75% lower than the control (w/o tobacco plant).

강원특별자치도 18개 시군을 대상으로 감나무와 콩에 발생하는 미국선녀벌레, 썩덩나무노린재, 담배거세미 나방의 발생을 조사하였다. 돌발해충인 미국선녀벌레는 약충기인 6월과 성충기인 10월에 발생조사를 진행하고 남방계해충인 담배거세미나방과 썩덩나무노린재는 7월과 9월 콩 재배기에 발생조사를 진행하였다. 미국선녀 벌레는 강원지역에 있는 감나무에서 발생을 확인할 수 없었고. 감나무 주변 기타 수목에서 가지당 평균 6.7마리 발생하였다. 담배거세미나방은 7월, 속초를 제외한 모든 시군에서 발생이 되었으며, 춘천, 횡성에서 트랩당 60마 리가 넘는 발생밀도를 보였으며, 원주, 강릉, 화천 등 에서는 트랩당 1마리로 낮은 발생밀도를 나타냈다. 썩덩나무 노린재는 7월 춘천, 속초, 홍천 등 7개 시군을 제외한 11개 시군에서 트랩에 포획되는 것을 확인하였으며, 화천에 서 트랩당 26마리로 최고발생밀도를 보였다.

Callipogon relictus, a natural monument, is an insect whose life cycle was assumed to take more than 5 years in nature. Winter is a very harsh season, but it is known to be a crucial condition for many insects’ growth. However, no information is known about the overwintering condition and its effects on C. relictus. To understand the overwintering effects on the growth of C. relictus, we investigated the growth patterns of its larvae in indoor conditions after chilling treatment. The larvae were induced to dormancy at low temperature (4°C) for two months, and put them into 10°C for two weeks to break dormancy. After awakening, the temperature was increased 15°C, 20°C to 25°C at a time, and the larvae were kept for two weeks at each temperature. The larvae were divided into 3 groups (3rd to 5th instar, 6th to 8th instar, 9th to last instar). Lastly, head width and weight of the larvae were measured every 30 days under 25°C condition, and mortality and deformity were counted as well. The mortality and deformity rates were the highest in the first group, and the rates decreased toward the last group. On the other hands, growth rate appeared opposite to mortality and deformity rate of each larval group.

The fall armyworm (FAW), Spodoptera frugiperda (Lepidoptera: Noctuidae), which is native to tropical and subtropical regions of the Western Hemisphere is now annually arrives in Korea. In this study, we developed loop-mediated isothermal amplification (LAMP) assay, one of the main merits of which is a rapid identification of target species. Five among 11 FAW-specific loci tested successfully provided a consistent reaction when ten FAWs, which were collected from eight localities in four countries were tested, whereas the 13 non-target species were not amplified. To increase in-field applicability of the method all life stages, reaction time, and different periods after death was tested using the quick extracted DNA. Our FAW diagnostic protocol can be completed within 30 min, from the process of extracting genomic DNA from an egg or a 1st instar larva to species determination.