Background: Leydig cells, crucial for testosterone production, express ion channels like ANO1 that influence hormone secretion. This study investigates the expression and role of the Tandem of P domains in a weak inward rectifying K+ channel-related Acid-Sensitive K+-1 (TASK-1) channel in these cells, exploring its impact on testicular function and steroidogenesis. Methods: TASK-1 expression in Leydig cells was confirmed using immunostaining, while RT-PCR and Western Blot (WB) validated its expression in the TM3 Leydig cell line. The effect of a TASK-1 channel blocker on cell viability was assessed through live/dead staining and MTT assays. Additionally, the blocker’s effect on testosterone secretion was evaluated by measuring testosterone levels. Results: Immunohistochemical analysis revealed a predominant presence of TASK- 1, along with c-Kit and ANO-1, in Leydig cells adjacent to seminiferous tubules and also in Sertoli and spermatogenic cells. Expression levels of TASK-1 mRNA and protein were significantly higher in TM3 Leydig cells compared to TM4 Sertoli cells. In addition, blocking TASK-1 in TM3 cells with ML365 induced cell death but did not affect LHinduced testosterone secretion. Conclusions: These findings suggest that TASK-1 in Leydig cells is crucial for their viability and proliferation, highlighting its potential importance in testicular physiology.

We produced an activated carbon using sodium-lignosulfonate, in which we investigated how the sodium salt in lignin served as the activating agent during heat treatment. Our process resulted in a product with a high specific surface area of 1324 m2/ g at 800 °C and microporous structure. During the activation process, we observed the consumption of carbon due to the dehydration reaction of NaOH and the reduction of Na2CO3 to metallic Na, which created pores through oxidation/ reduction reactions. The intercalation of metallic Na between the lattices at high temperatures formed additional pores and increased the specific surface area. Our proposed mechanism holds promise for enhancing the control of the microstructure and porosity of activated carbons through the thermal treatment of biomass.

Predictive maintenance has been one of important applications of data science technology that creates a predictive model by collecting numerous data related to management targeted equipment. It does not predict equipment failure with just one or two signs, but quantifies and models numerous symptoms and historical data of actual failure. Statistical methods were used a lot in the past as this predictive maintenance method, but recently, many machine learning-based methods have been proposed. Such proposed machine learning-based methods are preferable in that they show more accurate prediction performance. However, with the exception of some learning models such as decision tree-based models, it is very difficult to explicitly know the structure of learning models (Black-Box Model) and to explain to what extent certain attributes (features or variables) of the learning model affected the prediction results. To overcome this problem, a recently proposed study is an explainable artificial intelligence (AI). It is a methodology that makes it easy for users to understand and trust the results of machine learning-based learning models. In this paper, we propose an explainable AI method to further enhance the explanatory power of the existing learning model by targeting the previously proposedpredictive model [5] that learned data from a core facility (Hyper Compressor) of a domestic chemical plant that produces polyethylene. The ensemble prediction model, which is a black box model, wasconverted to a white box model using the Explainable AI. The proposed methodology explains the direction of control for the major features in the failure prediction results through the Explainable AI. Through this methodology, it is possible to flexibly replace the timing of maintenance of the machine and supply and demand of parts, and to improve the efficiency of the facility operation through proper pre-control.

There have been a lot of studies in the past for the method of predicting the failure of a machine, and recently, a lot of researches and applications have been generated to diagnose the physical condition of the machine and the parts and to calculate the remaining life through various methods. Survival models are also used to predict plant failures based on past anomaly cycles. In particular, special machine that reflect the fluid flow and process characteristics of chemical plants are connected to hundreds or thousands of sensors, so there are not many factors that need to be considered, such as process and material data as well as application of derivative variables. In this paper, the data were preprocessed through time series anomaly detection based on unsupervised learning to predict the abnormalities of these special machine. Next, clustering results reflecting clustering-based data characteristics were applied to produce additional variables, and a learning data set was created based on the history of past facility abnormalities. Finally, the prediction methodology based on the supervised learning algorithm was applied, and the model update was confirmed to improve the accuracy of the prediction of facility failure. Through this, it is expected to improve the efficiency of facility operation by flexibly replacing the maintenance time and parts supply and demand by predicting abnormalities of machine and extracting key factors.

It has been reported that gastrocnemius tightness and posterior talar glide are crucial factors influencing ankle dorsiflexion. However, the relationship of ankle dorsiflexion and these factors is not identified in previous studies. The purpose of this study was to identify the relationship of ankle dorsiflexion passive range of motion and gastrocnemius tightness and posterior talar glide. Twenty-five male subjects participated in this study. Bilateral weight-bearing ankle dorsiflexion passive range of motion and amount posterior talar glide of participants were measured using an inclinometer. Change in myotendinous junction of medial gastrocnemius was measured using ultrasonography to identify gastrocnemius tightness. Pearson product moment correlations were performed to examine correlations between ankle dorsiflexion passive range of motion and gastrocnemius tightness and posterior talar glide. Present findings revealed significant correlation between ankle dorsiflexion passive range of motion and gastrocnemius tightness (p=0.017, r=0.336). Also, ankle dorsiflexion passive range of motion was correlated with posterior talar glide (p=0.001, r=0.470). The present findings provide experimental evidence for factors influencing weight-bearing ankle dorsiflexion.

The purpose of this study was to investigate the effects of squat exercises with an upper limb resistance movement on the vastus medialis oblique, vastus lateralis, and gluteus maximus. This study recruited 16 healthy men. All subjects performed squat exercises with and without upper Limb resistance movements by using elastic tubing band. Electromyography (EMG) activity of the vastus medialis oblique, vastus lateralis, and gluteus maximus was recorded using a surface EMG system during these isometric squat exercises in both experimental conditions. The different muscle activation of the vastus medialis oblique, vastus lateralis, and gluteus maximus between the squat exercises with and without the upper limb resistance movement was analyzed using a paired t-test or Wilcoxon signed rank test. Our findings showed that the vastus medialis oblique, vastus medialis oblique/vastus lateralis ratio and gluteus maximus muscle activity was significantly increased during the squat exercise with upper limb resistance movements, as compared to the squat exercise without this component (p < .05). Based on our findings, the additional upper limb resistance movement may be a useful strategy to facilitate the vastus medialis oblique and gluteus maximus muscle activation during a squat exercise.

방울토마토의 수경재배 중 붕소+칼슘+규소 및 칼슘+규소의 복합 엽면시비가 수확 후 품질과 MAP 저장 중 저장성에 미치는 영향을 알아보고자 본 연구를 실시하였다. 엽면시비한 방울 토마토(‘Unicorn’)는 반숙 과상태에서 수확하여 산소투과성 필름으로 포장한 5oC, 11oC, 그리고 24oC에서 25일, 15일, 10일간 저장하였다. 붕소+칼슘+규소 복합처리한 방울토마토가 3가지 저장온도 모두에서 호흡과 에틸렌 발생이 억제되어 MAP 저장중 가장 낮은 생체중 감소와 가장 높은 외관상 품질을 보였다. 수확 후 조사한 방울토마토의 경도, 산도, 비타민 C 함량은 붕소+칼슘+규소 복합처리에서 가장 높았으며, 3가지 온도 모두에서 MAP 저장 후에도 모두 높게 유지되었다. 그러나 과피색, 라이코펜 함량과 당도는 수확 후에는 엽면시비 처리로 차이가 없었으나, 3가지 온도 모두 붕소+칼슘+규소 복합처리에서 가장 낮은 수치를 보였다. 이상의 결과로 볼 때 붕소+칼슘+규소 복합처리는 방울토마토의 수확후 생리 작용을 억제하고 경도, 산도, 비타민 C 함량을 높여 저장성을 향상시키는 것으로 판단되었다

Adhesive capsulitis of the shoulder is a common cause of pain that occurs during shoulder movement, thereby restricting shoulder rotation in clinical practice. Although most patients respond to pain relief treatment (NSAID or corticosteroids) by improving their range of motion, it remains poorly understood without any definitive treatment algorithm. In addition to immune cells, synoviocytes, chondrocytes and osteoblasts in the joint are known to produce pro-inflammatory mediators such as reactive oxygen species (ROS), inflammatory cytokines and lipid mediators, presumably contributing to the pathogenesis of osteoarthritis (OA) and adhesive capsulitis. Although inflammation and also fibrosis are proposed to be the basic pathological changes of a frozen shoulder, there is a lack of information regarding the downstream targets of the pro-inflammatory ROS signaling pathway in the synoviocytes and also how these ROS targets are modulated at the transcription level by a corticosteroid - dexamethasone. In this study, we used human fibroblast like synoviocytes (HFLS) to characterize the signaling targets of ROS by employing a human DNA microarray tool and studied the role of dexamethasone in this process. Our data suggest that several genes such as FOS, FOSB and NFkBIZ, which are known to be involved in pro- or anti- inflammation response, are modulated at the transcription level by ROS and dexamethasone.

The purpose of this study was to examine the effects of abdominal hollowing with ultrasound biofeedback on multifidus and transversus abdominis/internal oblique muscle activities during lifting tasks. This study recruited 12 healthy men. All subjects received training on expiration without and with abdominal hollowing with ultrasound biofeedback. After receiving training for each condition, the thickness of transversus abdominis was measured using ultrasound, and then, electromyography activities of transversus abdominis/internal oblique and multifidus during lifting tasks were measured using a surface electromyography system. The differences in thickness of transversus abdominis and electromyography activities of transversus abdominis/ internal oblique and multifidus between the two conditions were analyzed using paired t-test. Thickness of transversus abdominis and transversus abdominis/internal oblique and multifidus activities were significantly increased during expiration with abdominal hollowing with ultrasound biofeedback compared to during expiration without abdominal hollowing (p < 0.05). These findings suggest that abdominal hollowing with ultrasound biofeedback is effective in increasing trunk stabilizer muscle activities during both static and dynamic tasks such as lifting.

The population size of Red-spotted Apollo Butterfly(Parnassius bremeri) has been reduced because of their habitats destruction and partly climate change. Estimation of metapopulation size and survival day of Red-spotted Apollo Butterfly was made in Samcheok where release was carried for 5 years, Korea, by using the mark-release-recapture method. 421(female: 188, male: 233) of Red-spotted Apollo Butterfly were captured and 177 individuals(female: 89, male: 88) were recaptured and rates of recapture was 42%. Average of survival day was 3.59 and max survival day was 11. The migration of Red-spotted Apollo Butterfly was occurred significantly between short patches. Their max distance of migration was 6.74km. Estimate of P. bremeri was from minimum 125 to maximum 1844.

Medical mushroom, Phellinus linteus and Phellinus baumii called as “Sanghwang” have cultivated in Korea. PL has been studied extensively for its extraordinary capacity of suppressing cancer or enhancing body immunity. The mycelial materials of PL have mainly been used as research samples worldwide because fruiting bodies was difficult to be artificially cultivated. Alternatively, P. baumii (variety, ‘Jangsu’) have been cultivated in Korea. However, fruiting body morphology of P. baumii is clearly different to that of PL. Generally, Phellinus spp. including P. linteus slowly grow on artificial medium such as Potato Dextrose Agar (PDA). In contrast, P. baumii strains were rapidly grown on the artificial media when compared to other Phellinus spp. and thus it was considerable that its mycelial growing ability can be acted as a factor for producing fruiting bodies. This study aimed to find Phellinus isolates having high mycelial growth rate. Five Phellinus isolates that show rapid growth rate on YGM medium were selected from 36 Phellinus isolates collected in Korea. They were identified on nucleotide sequences of rDNA-ITS region. Phellinus linteus strain and Phellinus spp. showing mycelial growth rate comparing to P. baumii were characterized on cultural and bioactive characteristics (antioxidant activity and immune activation).