Caprine cryptosporidiosis mainly occurs in young goats, with morbidity rates of 80%–100% and mortality over 50% in goat kids. However, limited research has been conducted on the impact of Cryptosporidium parvum, a diarrhea-causing pathogen, on the intestinal microbiota of goat kids. In this study, 16S rRNA-based metataxonomic analysis was performed to compare the microbial diversity and abundance of the gut microbiota between C. parvum-infected and uninfected goat kids. In total, 12 goat fecal samples were collected, including seven naturally C. parvum-infected and five uninfected goats from Chungcheongbuk-do, Korea. After amplification of the V3–V4 hypervariable region of the bacterial 16S rRNA, high-throughput sequencing was performed. The results showed differences in the microbial composition between C. parvum-infected and uninfected groups based on beta diversity. Firmicutes and Bacteroidetes were the most dominant phyla in both groups. However, no significant difference was observed in the Bacteroidetes/Firmicutes ratio between the two groups. Compared with the uninfected group, the C. parvum-infected group showed significantly higher abundances of Tyzzerella nexillis, Lactobacillus johnsonii, Butyricicoccus pullicaecorum, Enterococcus raffinosus, Enterococcus faecalis, and Negativicoccus massiliensis, and significantly reduced abundances of Aerococcus vaginalis, Faecalicoccus pleomorphus, Oribacterium parvum, and Coprococcus comes. These findings indicate that C. parvum infection, which is associated with diarrhea in neonatal goats, induces alterations in the caprine gut microbiota.

The importance of urban green space creation is increasingly recognized as the most realistic and efficient approach for fine dust mitigation in urban areas. Particularly considering the characteristics of domestic cities, the application of buffer green spaces along roads can maximize the efficiency of fine dust reduction without the need for separate green space creation. Accordingly, this study analyzed the fine dust mitigation effects based on the types of plantings in the central dividers and roadside trees in Jeonju City, Jeollabuk-do. To do this, we controlled various external variables of urban space and considered the planting arrangement types in the central dividers, carrying out the analysis using a CFD simulation. The simulation results confirmed that the central dividers with plantings demonstrated more effective ultrafine dust reduction than those without. Moreover, the arrangement of roadside trees showed a greater ultrafine dust reduction effect when adopting a multilayered structure compared to a single layer. Based on these findings, we concluded that installing both trees and shrubs simultaneously in the central dividers and along roads was effective for ultrafine dust mitigation. On this basis, we quantified the dust reduction effects of plants in urban street environments and proposed planting guidelines for roadside green spaces to improve air quality.

본 연구에서는 육계사에 차열 페인트와 히트펌프의 적용에 따른 내부 온도 변화를 분석 하였다. 이를 위하여 환기율, 환기 방법, 시간별 환기 변화에 따른 실험 조건을 설정하였으며 육 계사 외부 및 내부 기온을 측정하였다. 그 결과, 차열 페인트를 도포한 육계사에서는 최대 1－2°C 실내 기온 상승을 억제하 는 효과가 나타났으며 히트펌프를 가동한 육계사에서는 외기 온도의 영향을 제일 적게 받는 환기율 0%일 때 내부 기온 감소 가 제일 크게 나타났다. 계사 내부의 온도가 외기 온도보다 높 을 경우에는 환기율을 높게 설정하여 환기팬을 이용한 냉방이 더욱 효과적이나 계사 내부 온도가 외기 온도와 유사하거나 낮을 경우에는 히트펌프를 이용하는 것이 가장 효과적일 것으 로 판단된다. 히트펌프 가동 시 외기 온도의 영향이 적은 환기 율을 0%로 설정하였을 때 내부 기온이 가장 큰 폭으로 감소하 였으나 실제 육계사에서는 분진, 이물질, 암모니아 등을 고려 하여 최소환기율 정도로 환기율을 설정한 후 히트펌프를 가동 하는 것이 가장 효율적일 것으로 판단된다. 본 연구는 실험 기 간이 짧아 데이터가 많지 않으며 실제 육계가 사육되고 있는 환경에서 실험을 진행한 것이 아니라는 한계가 있다. 향후 후 속 연구로 실제 닭이 사육되고 있는 환경에서의 히트펌프 효 과 분석과 히트펌프의 전력사용량, 냉방부하, 환기팬 가동시 간 등 다양한 환경인자를 포함한 연구가 진행되어야 할 것으 로 판단된다.

수소는 다양한 신재생에너지 중 환경친화적인 에너지로 각광받고 있지만 농업에 적용된 사례는 드물다. 본 연구는 수소 연료전지 삼중 열병합 시스템을 온실에 적용하여 에너지를 절 약하고 온실가스를 줄이고자 한다. 이 시스템은 배출된 열을 회수하면서 수소로부터 난방, 냉각 및 전기를 생산할 수 있다. 수소 연료 전지 삼중 열 병합 시스템을 온실에 적용하기 위해 서는 온실의 냉난방 부하 분석이 필요하다. 이를 위해서는 온 실의 형태, 냉난방 시스템, 작물 등을 고려해야 한다. 따라서 본 연구에서는 건물 에너지 시뮬레이션(BES)을 활용하여 냉 난방 부하를 추정하고자 한다. 전주지역의 토마토를 재배하 는 반밀폐형 온실을 대상으로 2012년부터 2021년까지의 기 상데이터를 수집하여 분석했다. 온실 설계도를 참고하여 피 복재와 골조를 모델화하여 작물 에너지와 토양 에너지 교환을 실시했다. 건물 에너지 시뮬레이션의 유효성을 검증하기 위 해 작물의 유무에 의한 분석, 정적 에너지 및 동적 에너지 분석 을 실시했다. 또한 월별 최대 냉난방 부하 분석에 의해 평균 최 대 난방 용량 449,578kJ·h-1, 냉방 용량 431,187kJ·h-1이 산정 되었다.

Ticks and tick-borne pathogens are among the main sources of interest in veterinary medicine and public health. This review aimed to cover published data on tick species and their geographical distribution in Korea. Over 143 articles regarding ticks were published between 1966 and 2022, and reporting at least 29 species belonging to the family Ixodidae and five belonging to the family Argasidae. Among these, Haemaphysalis longicornis, H. flava, and Ixodes nipponensis were the main genera distributed nationwide, whereas Amblyomma was mostly identified in the southern region. Conversely, only a limited number of Argasidae ticks were present, including the genera Argas and Ornithodoros. Considering the changes in tick distribution patterns due to global warming, continuous nationwide monitoring of ticks is required.

Pluripotent stem cells could self-renew and differentiate into various cells. In particular, porcine pluripotent stem cells are useful for preclinical therapy, transgenic animals, and agricultural usage. These stem cells have naïve and primed pluripotent states. Naïve pluripotent stem cells represented by mouse embryonic stem cells form chimeras after blastocyst injection. Primed pluripotent stem cells represented by mouse epiblast stem cells and human embryonic stem cells. They could not produce chimeras after blastocyst injection. Populations of embryonic stem cells are not homogenous; therefore, reporter systems are used to clarify the status of stem cells and to isolate the cells. For this reason, studies of the OCT4 reporter system have been conducted for decades. This review will discuss the naïve and primed pluripotent states and recent progress in the development of porcine OCT4 reporter systems.

본 연구는 가스추진 174K급 LNG 운반선의 가스 압축기실에서 발생하는 가스누출 모사를 통해 가스탐지기의 최적 위치를 분석 하였으며, 새로 개정된 IGC 코드에 명시된 안전규정을 만족하는 합리적인 방법도 함께 제안하였다. 가스압축기실에서의 LNG 가스누출 수치해석을 위해, 실제 ME-GI 엔진이 장착된 174K급 LNG 운반선의 압축기실 형상과 장비, 배관의 배치와 같은 치수로 3D 설계되었다. 가 스누설에 대한 시나리오는 305 bar의 높은 압력과 1 bar의 낮은 압력을 적용하여 진행하였다. 고압용 핀홀의 크기는 4.5, 5.0, 5.6 mm이고 저압용은 100, 140 mm이다. 해석 결과, 5.6 mm 핀홀(고압)과 100, 140 mm 핀홀(저압) 상태의 누출에 대한 환기평가에서 가연성 가스농도는 심 각한 위험이 없음을 확인하였다. 그러나 개정된 IGC 코드에 따라 설치된 압축기실의 가스 감지 센서의 실제 위치는 다른 지점으로 이동 해야 하고, 측정 지점이 현 규정에서 요구하는 것보다 더 추가되어야 함을 확인하였다.

The transcription factor POU5F1, also known as OCT4 plays critical roles in maintaining pluripotency during early mammalian embryonic development and in embryonic stem cells. It is important to establish an OCT4 promoter region-based reporter system to study pluripotency. However, there is still a lack of information about the porcine OCT4 upstream region. To improve our understanding of the porcine OCT4 regulatory region, we identified conserved regions in the porcine OCT4 promoter upstream region by sequence-based comparative analysis using various mammalian genome sequences. The similarity of nucleotide sequences in the 5' upstream region was low among mammalian species. However, the OCT4 promoter and four regulatory regions, including distal and proximal enhancer elements, had high similarity. The putative transcription factor binding sites in the Oct4 5' upstream region nucleotide sequences from mice and pigs also differed. Some of these genes are related to pluripotency, and further research will allow us to better understand the differences in species-specific pluripotency. Next, a functional analysis of the porcine OCT4 promoter region was conducted. Luciferase reporter assay results indicated that the porcine OCT4 distal enhancer and proximal enhancer were highly activated in mouse embryonic stem cells and embryonic carcinoma cells, respectively. Similar to OCT4 upstream-based reporter systems derived from other species, the porcine OCT4 upstream region-based reporter constructs showed exclusive expression patterns depending on the state of pluripotency. This work provides basic information about the porcine OCT4 upstream region and various porcine OCT4 fluorescence reporter constructs, which can be applied to study species-specific pluripotency in early embryo development and the establishment of embryonic stem cells in pigs. This research was supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education (NRF-2017R1D1A1B03032256).

Polyethersulfone (PES) 고분자 상변환막의 성능을 향상시키기 위해 PES 고분자에 나노 크기의 ZnO 무기입자를 함침시킨 혼합기질막(mixed matrix membrane)을 제조하고 특성을 평가하였다. PES-ZnO 혼합기질막은 ZnO 나노입자를 PES 대비 최대 0.375 wt%의 낮은 비율로 첨가시킨 PES-ZnO-NMP(N-methyl-1-pyrrolidone)로 이루어진 캐스팅 용액을 사용하여 상변환법을 통해 제조하였다. 제조된 혼합기질 막의 물성과 특성은 막의 단면구조 관찰, 접촉각 측정, 인장강도 측정, 순수 투과량 측정 및 BSA 단백질 용액의 한외여과 실험을 통해 평가하였다. 이 결과 혼합기질 막은 PES 고분자 matrix에 함유된 ZnO 나노입자로 인해 막의 친수성이 증가하여 막오염 발생이 억제되어 투과량이 증가하였다. ZnO 나노입자는 혼합기질막의 제조에 있어 막오염의 발생 억제와 투과량 증가에 유용하게 사용될 수 있는 무기물 첨가제임을 알 수 있다.

Stimuli-responsive biomaterials that alter their function through sensing local molecular cues may enable technological advances in the fields of drug delivery, gene delivery, actuators, biosensors, and tissue engineering. In this research, pH-responsive hydrogel which is comprised of dimethylaminoethyl methacylate (DMAEMA) and 2-hydroxyethyl methacrylate (HEMA) was synthesized for the effective delivery of doxorubicin (Dox) to breast cancer cells. Cancer and tumor tissues show a lower extracellular pH than normal tissues. DMAEMA/HEMA hydrogels showed significant sensitivity by small pH changes and each formulation of hydrogels was examined by scanning electron microscopy, mechanical test, equilibrium mass swelling, controlled Dox release, and cytotoxicity. High swelling ratios and Dox release were obtained at low pH buffer condition, low cross-linker concentration, and high content of DMAEMA. Dox release was accelerated to 67.3% at pH 5.5 for 6-h incubation at 37oC, while it was limited to 13.8% at pH7.4 at the same time and temperature. Cell toxicity results to breast cancer cells indicate that pH-responsive DMAEMA/HEMA hydrogels may be used as an efficient matrix for anti-cancer drug delivery with various transporting manners. Also, pH-responsive DMAEMA/HEMA hydrogels may be useful in therapeutic treatment which is required a triggered release at low pH range such as gene delivery, ischemia, and diabetic ketoacidosis.

우리나라 경제의 성장을 이끈 기계산업은 2015년 현재 생산액 100조원, 사업체수 9,526개사, 종사자수 31만 5천명, 부가가치 39조원으로써 제조업 중 생산액 5위, 사업체수 2위, 종사자수 3위, 부가가치 4위에 위치하고 있다. 이러한 기계산업에 있어 공작기계를 이용해 금속을 원하는 모양으로 만드는 금속가공은 반드시 필요한 공정으로 금속가공 시 가공 장비의 수명 연장 및 가공물의 품질 향상을 위해 칩이 형성되는 영역에 적용되어 윤활 및 냉각작용을 하는 절삭유(Soluble Cutting Fluids, SCF)가 이용되고 있다. 절삭유는 수용성 및 비수용성으로 나뉘는데 비수용성 절삭유의 경우 윤활성은 우수하나 경제성 및 발연, 발화등의 문제점이 있으며, 수용성 절삭유는 윤활성은 비교적 떨어지나 냉각성과 경제성이 우수하다는 장점이 있다. 또한 절삭유는 기능 향상을 위해 PAH, 방부제, 파라핀계 염소, 아질산염 등 20~30개의 첨가제가 사용된다. 이는 중추신경계 장애, 이비인후 자극, 피부염 발생 등을 일으킬 위험성이 크다고 알려져 있으며, 고농도의 유기성분 및 질소화합물로 인해 수계에 유출시 부영양화, 녹조현상 등과 같은 문제를 일으킬 수 있다. 따라서 본 연구에서는 전기화학적 처리를 통해 반응시간에 따른 폐수용성 절삭유의 COD 및 T-N의 제거율 변화를 분석하였다. 전기화학적 처리의 중요 인자인 전극은 비교적 가격이 저렴하고 용출량이 낮은 것으로 알려져 있는 SUS316전극을 이용하였으며 자세한 실험 조건을 Table 1에, 연구에 사용한 장치의 모식도를 Fig. 1에 나타내었다.

We selected lactic acid bacteria to reduce cholesterol levels in vitro and in vivo. Among the 140 strains tested, a strain ID9201 was shown to reduce cholesterol in the spent medium over 30% and to increase cholesterol in cell pellet. A strain ID9201 isolated from breast milk-fed Korean infant feces was identified as Enterococcus faecium on the basis of morphological analysis, biochemical analysis (API kit), and 16S rDNA sequence analysis. A freeze-dried powder of the strain was fed to Sprague-Dawley(SD) rats with high cholesterol diet for 6 wk. In the control group fed cholesterol enriched diet without probiotics, the serum cholesterol increased by 1.9 times in comparison with the initial value. Among the test group, ID9201 group showed the lowest cholesterol increase, 1.5 times of initial serum cholesterol, as in the blank group fed normal diet without probiotics. Based on these data, we assumed that ID9201 inhibited intestinal absorption of dietary cholesterol.

This study was conducted to establish the elaborate nitrogen fertilization method to enhance N use efficiency in direct-seeded rice on flooded paddy. The nitrogen uptake by rice plants was insignificant until 25 days after seeding, and increased gradually thereafter. During this early growth stage, rice plants absorbed only the 4~% of basal applied N, while the 45~% of N fertilizer remained in the paddy soil. The absorption of basal N by rice plants was almost completed at 46 days after application. Nitrogen top-dressed at 5-leaf stage was well matched to crop nutrient demand, so it could be absorbed so actively in 8days after application. As a result, we could cut down the amount of N fertilizer to 36~% of the basal N level without significant difference in yield. Plant recoveries of fertilizer 15N applied with different application timings were 7.8~% for basal, 9.4~% for 5-leaf stage, 17.1~% for tillering stage, and 23.4~% for panicle initiation stage, respectively. When urea was applied with nitrogen fertilization practice based on basal incorporation (BN), plant recovery of 15N at harvest was 31.0~% , which was originated from 13.7~% for grain, and 21.3~% of the fertilizer 15N remained in the soil, and the rest could be uncounted. Plant recovery of fertilizer 15N applied with nitrogen fertilization practice based on topdressing at 5-leaf stage (TN), where N rate was reduced by 18~% compared with BN, was 35.1~% (grain 15.6~% ), and 19.9~% of the fertilizer 15N remained in the soil, and the rest could be uncounted. TN showed a higher 15N recovery than BN because it was to apply N fertilizer at a time to well meet the demand of rice plant direct-seeded on flooded paddy. We concluded that TN would be the nitrogen fertilization method to enhance N use efficiency in direct-seeded rice on flooded paddy.