This paper examines a simple one-step and catalyst-free method for synthesizing carbon nanoparticles from aliphatic alcohols and n-hexane with linear molecule formations by using a stable solution plasma process with a bipolar pulse and an external resistor. When the external resistor is adopted, it is observed that the current spikes are dramatically decreased, which induced production of a more stable discharge. Six aliphatic linear alcohols (methanol- hexanol) containing carbon with oxygen sources are studied as possible precursors for the massive production of carbon nanoparticles. Additional study is also carried out with the use of n-hexane containing many carbons without an oxygen source in order to enhance the formation of carbon nanoparticles and to eliminate unwanted oxygen effects. The obtained carbon nanoparticles are characterized with field emission-scanning electron microscopy, energy dispersive X-ray spectroscopy, and Raman spectroscopy. The results show that with increasing carbon ratios in alcohol content, the synthesis rate of carbon nanoparticles is increased, whereas the size of the carbon nanoparticles is decreased. Moreover, the degree of graphitization of the carbon nanoparticles synthesized from 1-hexanol and n-hexane with a high carbon (C)/oxygen (O) ratio and low or no oxygen is observed to be greater than that of the carbon nanoparticles synthesized from the corresponding materials with a low C/O ratio.

This report is a part of research on pests occurring in grapevine orchards in export complexes (Hwangsung in Gyeonggi, Sangju and Yeongcheon in Gyeongbuk, Namwon in Junbuk and Yeongdong in Chungbuk) from 2010 to 2012. This research was conducted to evaluate the distribution and difference in damage rates depending on management types of grapevine orchards (domestic sale farm vs. export farm). Damage by Arbordia spp. occurred only in 2010 and differed depending on localities and individual farms in the same area. Numbers of orchards damaged by Arbordia spp. were one, two and four in Hwasung, Namwon and Sangju, respectively, and the damage rate was below 6.2%. There was no damage in the orchards in 2011 and 2012, however, Arbordia spp. were collected on sticky traps in the orchards. A. nigrigena and A. kakogawana were the dominant species in Yeongcheon and Yeongdong, respectively, in 2011. A. kakogawana, A. maculifrons and A. nigrigena were collected on sticky traps in 2012. Collected numbers of Arbordia spp. were different depending on localities and management types of the orchards (domestic sale vs. export). A. kakogawana was the dominant species in all the survey sites and the densities were higher in the domestic sale farms than in the export farms.

The freemartinism is the most frequent form of intersexuality found in cattle, and females of heterosexual twins become sterile. With increase of twinning rates due to transfer of multiple embryos derived from in vitro fertilization, it is of great economic value to establish early diagnosis of freemartins to remove infertile individuals from breeding stock. In the present study polymerase chain reaction (PCR) of two different Y-chromosome specific segments (BRY.l and AMX/Y) was performed to identify freemartins from twins and less common single born freemartins in Korean Native Cattle (KNC). Two male-specific sequences were amplified in all heterosexual twins tested (n=5). In addition, Y-specific PCR products were detectable in one of the single born females (n=4) with visible genital abnormalities. These results suggest that the sensitivity of PCR-based assay may be sufficient to detect freemartinism in single born females as well as female partners of heterosexual twins in KNC.

Background : The purpose of this study was to evaluate methods to high quality food ramie rice cake, thereby increasing farm income. This study investigated the effects of different Split Application and Adapted Fertilizer Culture for Stable Production with Edible Leaf in Ramie (Boehmeria nivea L.). Methods and Results : Method of fertilizer application were conducted under three condition compose to standard application (N-P-K-Compost applied at 27-9-27-1,500 ㎏․ 10 a−1), 25% increased application (N-P-K-Compost applied at 34-11-34-1,500 ㎏․10 a−1), 50% increased application (N-P-K-Compost applied at 41-14-41-1,500 ㎏․ 10 a−1) cultivation. Compost and fused phosphate applied at 100% of basal fertilizer in March 25. The split application level of standard application (N-K) applied at 50% of basal fertilizer in March 25, 50% of top dressing were five times application (N-K) in May 4, July 5, September 5, October 5, 20% respectively. Transplanting with Yeongkwang varieties in April 12 by growing pot seedling for 35 days in 2011. Planting density were spaced 60 ㎝ apart in rows 25 ㎝ apart with rice straw cutting covered open cultivation. Plot design randomized block 3 repetition. Conclusion : Organic matter, available phosphate, K2O, CaO, and MgO of soil increased in all plots with the addition of fertilizers culture, specifically in 25% and 50% increased application. Growth characteristics by 50% of top dressing were five times application (N-K) in May 4, July 5, September 5, October 5, compared to standard application (N-K) applied at 100% basal fertilizer, aerial part as a result were so many such amount of growth as number of branch and leaf per plant, etc., Length, width of leaf and leaf colorimetry of aboveground part growth increment rising highest. Green leaf yields compared to standard fertilizer 100% application (1,344 kg․ 10 a−1). 25% and 50% increased application (N-K) applied at 50% basal fertilizer, 50% of top dressing were five times application increased by 9%, 17%. respectively.

Background : The purpose of this study was to evaluate methods to high quality medicinal vegetables, thereby increasing farm income. This study investigated the effects of different green manures crop and application culture stable foliage production in Peucedanum japonicum Thunberg. Methods and Results : Method of application with green manure crop were conducted under four condition compose to control (non application), single cropping in barley, hairy vetch and mixed cropping in hairy vetch (60%) + barley (40%)culture. Sowing dates of green manure crop were October 14 in 2014. Transplanting with Jeonam Yeosu varieties in Junel 28 by growing pot seedling for 45 days in 2015. Method of application were standard application (N-P-K-Compost applied at 20-12-10-600 ㎏․10 a−1). Plants were spaced 10 ㎝ apart in rows 10 ㎝ apart with open cultivation. Conclusions : The utilization effect of nitrogen supplied by green manure crop was highest with the hairy vetch by 21.1% in single cropping and hairy vetch + barley by 29.8% in mixed cropping. Organic matter, available phosphate, K2O, CaO, and MgO of soil increased in plots with the mixed cropping hairy vetch + barley culture. Non application < barley < hairy vetch < hairy vetch + barley in mixed cropping, aerial part as a result were so many such amount of growth as number of leaf per plant, etc., green leaf yields is untreated control plot (1,876 kg․10 a−1) compared to 7% from using barley culture, hairy vetch culture 10% higher and hairy vetch + barley culture increased to 15% of the most.

Background : The objective of this study was to Amomum tsao-ko Crevost et Lemarié, (Zingiberaceae) is widely distributed among several countries in South Asia and Southeast Asia. It is a well known spice in Asia, produces a nice refreshing effect in the mouth. Additionally, it's dried fruit is used in Traditional Chinese Medicine (TCM) for cardiac diseases, edema, eye trouble, skin, itch and impotence. The objective of this study was evaluated the inhibitory activity on adipogenesis in 3T3-L1 cells from A. tsao-ko. Methods and Results : The fruits of A. tsao-ko were extracted with 80% EtOH two times at room temperature. The EtOH extract was suspended in distilled water and partitioned with solvent to give CH2Cl2, EtOAc and n-BuOH. The CH2Cl2 was suspended in n-hexane and partitioned with solvent to give 50%, 70% and 90% MeOH. The purification of each fraction by column chromatography separation and HPLC analysis. Consequently, several constituents were isolated five known compounds. The identification and structural elucidation of compounds were established by using NMR (1D and 2D) and mass spectrometry. Conclusion : These compounds were identified as fluorenone (1), phenanthrene (2), anthracene (3), methyl linolenate (4), 1,2-benzenediol (5). All isolates were tested for their inhibitory activities on adipogenesis in 3T3-L1 cells.

Background : Amomum tsao-ko (Zingiberaceae) is widely distributed among several countries in Asia. It’s dried fruit is widely used in Korea for medical plant, China and Japan for the treatment of dyspepsia, eliminates, vomiting, abdominal pain, phlegm, warms the spleen, and malaria. In this study, we describe the structural determination of the new compounds and the inhibitory activities of isolated compounds against LPS-induced NO production in RAW264.7 cells. Methods and Results : The fruits of A. tsao-ko were extracted with 80% EtOH two times at room temperature. The EtOH extract was suspended in distilled water and partitioned with solvent to give CH2Cl2, EtOAc and n-BuOH. The CH2Cl2 was suspended in n-hexane and partitioned with solvent to give 50%, 70% and 90% MeOH. The purification of each fraction by column chromatography separation and HPLC analysis. Consequently, one new benzaldehyde (1) and two new cycloterpenals (2 and 3) along with five known compounds (4 –8) have been isolated from the fruits of A. tsao-ko. The structure and relative stereochemistry were determined from HRMS, 1D and extensive 2D NMR techniques as well as by comparison of their data with the published values. Conclusion : These compounds were identified as Amotsaokonal A (1), Amotsaokonal B (2), Amotsaokonal C (3), methyl linolenate (4), trans-nerolidol (5), (2E)-dodecenyl acetate (6), (2E)-dodecenyl acetate (7), and pyrrole-2-carboxylic acid (8). All isolates were tested for their inhibitory activities on LPS-induced nitric oxide production in RAW264.7 cells.

Background : The purpose of this study is to investigate the effect of the ridge and mulching cultivation and adapted sowing(planting)time in stable medicinal herbs production of Lithospermum erythrorhizon Siebold et Zuc. And it accomplished one’s purpose to GAP guide manual established standard method of cultivation technology, contribute to the enlargement of farm’s income. Methods and Results : Method of cultivation type with the ridge and covering material were conducted under two condition compose to open cultivation (level row : 120㎝ ridging, black polyethylene mulching) and plastic greenhouse cultivation (high row : 90㎝ ridging, non-woven fabric mulching). Sowing with Daehong varieties in April 17, transplanting July 25 by growing pot seedling for 40 days in 2015. Planting density were spaced 30㎝ apart in inter-rows 10 ㎝ apart intra-row. Fertilizing level of N-P-K-Compost (fermented mixed oil cake fertilizer) applied at 6-4-6-300 ㎏․10 a−1, 60% of basal fertilizer in March 14, 40% of top dressing were four times application in April 14 ～July 15. Plot design randomized block 3 repetition. Conclusions : Compared to level ridging on black polyethylene mulching open cultivation for growing plastic greenhouse with the high ridging non woven mulching cultivation, seed yields havesting 1.4 times, the dried roots yields increased in 0.7 times. Also, Growth characteristics by sowing time in April 16. compared to planting time in July 25. Length of stem and main root of above-ground, subterranean part growth increment rising highest. Seed and dried roots yields with increased by 71%, 30%. respectively.

Background : The purpose of this study was to evaluate methods to high quality medicinal vegetables, thereby increasing farm income. This study investigated the effects of different covering material and weed eliminating on stable foliage production in Peucedanum japonicum Thunberg. Methods and Results : method of weed elimination with covering material were conducted under four condition compose to Non covering(4～6 times by manual weed control), Barley straw cutting covered with 700㎏․10 a−1, Rice straw cutting covered with 500㎏․10 a−1, Rice husks covered with 1,000㎏․10 a−1. Fermented mixed oil cake compost applied at 380㎏․10 a−1, 60% of basal fertilizer in March 14, 40% of top dressing were four times application in April 14 ～July 15. Sowing dates were March 25, 2015. Plants were spaced 15 ㎝ apart in rows 15 ㎝ apart with open cultivation. Conclusions : Rice husks covering < Barley straw covering < Barley straw covering cultivation this order, aerial part as a result were plenty amount of growth. Weed the rates was 12 to 29% lower incidence. Green leaf yields is uncovering control plot (915 kg․10 a−1) compared to 2% from covering rice husks, barley straw and rice straw covering increased to 18%, 20%, respectively.

Background : The purpose of this study was to evaluate methods to high quality medicinal vegetables, thereby increasing farm income. This study investigated the effects of different organic fertilizer and applicated cultivation on stable foliage production in Peucedanum japonicum Thunberg. Methods and Results : method of application with organic fertilizer were conducted under nine condition compose to control(non application), N-P2O5-K2O (20-12-10)㎏․10 a−1, Hyphal incubation compost(HIC) applied at 851㎏․10 a−1, Hangawi organic compost(HOC) applied at 547㎏․10 a−1, Fermented mixed oil cake compost(FMOC) applied at 380㎏․10 a−1, 60% and 40% of basal fertilizer in March 12, top dressing were four application in April 12, May 12, June 13 and July 12, respectively. Sowing dates were March 23, 2015. Plants were spaced 15 ㎝ apart in rows 15 ㎝ apart with open cultivation. Conclusions : chemical fertilizers < hyphal incubation compost < hangawi organic compost < fermented mixed oil cake compost application, top dressing than 60% in order of 40% additional fertilizer, aerial part as a result were so many such amount of growth as number of leaf per plant, etc., Green leaf yields is untreated control plot (875 kg․10 a−1) compared to 12% from using chemical fertilizers, HIC application 18% higher, HOC application 20% higher, and FMOC application increased to 28% of the most.

Seed color is an important factor affecting physiological and developmental process in wheat. One of the plant pigments, anthocyanins are a group of flavonoid compounds well known as pigments responsible for blue, purple, red, or yellow coloration of plant tissues. In this study, we investigated the pigmentation of purple and yellow color seed according to wheat grain developmental stages. The contents of anthocyanin and chlorophyll in the purple and yellow seeds were measured. Chlorophyll contents were changed similarly in both purple and yellow color seed, and no significant difference was observed between them. In purple color seed, the content of anthocyanin was significantly induced compared with yellow color seed. The individual anthocyanin components were investigated by ultra performance liquid chromatography (UPLC). Cyanidine-3-glucoside (C3G) and peonidine-3-glucoside (P3G) were detected as predominant anthocyanin in purple color wheat. To investigate whether structural genes in anthocyanin biosynthesis were involved in the trait differences between purple and yellow color seed, we examined the expression of anthocyanin biosynthesis-related genes (CHS, CHI, F3H, DFR, ANS, UFGT) and MYB transcription factor in developing wheat grains by using qRT-PCR. This study indicates that the expression of anthocyanin biosynthesis-related genes and MYB transcription factors correlate with anthocyanin levels of grain.

Ionizing radiation directly and indirectly affects gene expression within the plant genome. To access the physiological response of rice to different types of ionizing radiation, rice seeds were exposed to gamma-ray and ion beam radiation. Exposure to ionizing radiation dramatically decreased the shoot length compared with non-irradiated plants. Fluorescence-activated-cell-sorting (FACs) was used to measure DNA contents. There were significant correlations of dose-dependent between irradiated plant and non-irradiated plant. The radicals induced by the ionizing radiation in the plant could be observed by electron spin resonance (ESR). It was confirmed that the number of free radicals in cell was greatly increased all irradiated plants than non-irradiated plant. A significant positive correlation was shown between ionizing radiation dose and signal intensity. In order to determine the Genetic diversity, AFLP analysis was conducted with the irradiated plant and non-irradiated plant. Based on band patterns, the cluster analysis was conducted to evaluate the genetic variation by using the UPGMA (Unweighted Pair Grouping Method of Averages). Genetic diversity of irradiated plants by low dose ion beam was the closest non-irradiated plant and irradiated by high dose gamma-ray was the furthest from non-irradiated. We describe the detailed methods of ionizing irradiation and discuss its applications in genetic research as well as plant breeding.

Ionizing radiation affects gene expression from plant genomes. To monitor the genome-wide transcriptional changes induced by three types of ionizing radiation, we used the rice RNA sequencing to identify genes that are up- or down-regulated by gamma rays (GAs), proton (PRs) and ion beams (IBs). The Oryza sativa jacalin-like lectin domain containing proteins (OsJAC1) gene was highly induced by GAs, PRs and IBs. OsJAC1 was selected based on the expression patterns of a genome-wide dataset of RNA sequencing. Many jacalin-related lectin genes have been shown to be associated with disease resistance, biotic and abiotic stress signaling. Therefore, we studied its expression pattern in response to different abiotic stress and phytohormone treatments. The expression patterns of OsJAC1 under two different abiotic stress conditions (salt and heat stress) and phytohormones (salicylic acid and methyl jasmonate) were examined. The transcripts of OsJAC1 were significantly induced in response to abiotic stress conditions, including salt and heat treatments. In addition, it was induced in response to the salicylic acid and methyl jasmonate treatments, respectively. To investigate the sub-cellular localization of OsJAC1, the gene was expressed as a fusion protein tagged with GFP, in tobacco leaf epidermis and examined under confocal microscope. The OsJAC1 was clearly localized at the nucleus. These results provide critical insights into the molecular functions of the rice jacalin-like lectin domain containing proteins as receptors of external signals.

Space has many distinguishable characteristics from earth such as strong cosmic radiation, microgravity, supervaccum and weak magnetic field. For this reason, space environments can be used an efficient mutagen for plant breeding nowadays. To identify the affected genes by condition in space with outer space, Brachypodium seeds were placed in the Russia Segment (RS) Biorisk module of International Space Station (ISS). Brachypodium distachyon is a model system for temperature grass, because they represent the characteristics for annual winter grass. Seeds and organs of plants carried by satellite or spacecraft to space can be genetically mutated by exposing space environment. We performed a duplicated RNA sequencing to profile the differentially expressed genes. As a results, about 700 genes were upregulated and 250 genes were downregulated by cosmic environments, respectively. In the molecular function category, protein kinase and transcription activity related genes were upregulated. Among the many transcription factors (TFs), stress related TFs such as ERF, NAC and WRKY were differentially expressed in space exposed samples. In the future, their expression will be identified by using qRT_PCR.

‘Tocomi-1’, a new japonica rice cultivar derived from a 200 Gy gamma ray irradiation with high tocopherol content and red pericarp. The local adaptability test of MRXII-1001-1 was carried out from 2012 to 2014 and it was named as ‘Tocomi-1’ in 2014. This variety is medium matured with heading date of August 12 in honam plain area of Korea. This variety is about 80 cm tall culm length and 106 spikelets per panicle. Its 1,000 grain-weight of rice seeds is 25.4 g. The yield potential of this variety is about 5.15 MT/ha in local adaptability test for three years. This variety exhibited greater seed longevity than the Donganbyeo, indicating a crucial role for tocopherols in maintaining viability during quiescence, and displayed faster seedling growth during the early growth stage. Tocopherol contents was 50% higher than the Donganbyeo. To study the molecular mechanism underlying vitamin E biosynthesis, we examined the expression patterns of seven rice genes encoding vitamin E biosynthetic enzymes. Accumulation levels of the OsVTE2 transcript and OsVTE2 protein in the ‘Tocomi-1’ were significantly higher than in the Donganbyeo. Sequence analysis revealed that the ‘Tocomi-1’ harbored a point mutation in the OsVTE2 promoter region, which resulted in the generation of MYB transcription factor—binding cis-element. These results help identify the promoter regions that regulate OsVTE2 transcription, and offer insights into the regulation of tocopherol content in ‘Tocomi-1’.

Kenaf (Hibiscus cannabinus L.) native to Africa can be used as fiber, food, feedstock and bio plastic. This study was carried out to evaluate the mineral, amino acid and vitamin contents of six selected kenaf cultivars which are enable to produce seed under Korean circumstance. The leaves of three mutant cultivars (Jangdae, Jeokbong and Baekma), two original cultivars (Jinju, C14) and one Chinese cultivar (Auxu) were harvested at flowering time. Mineral components of kenaf leaves, such as calcium, potassium, and mineral, did not showed significant differences among the cultivars. As major amino acids including proline and phenylalanine, significant differences were found in these kenaf cultivars. The Auxu cultivar contained the highest amount of essential amino acid (Phenylalanine, Leucine, Isoleucine, Valine, Methionine and Lysine). The amount of vitamin displayed significant differences such as vitamin E and vitamin K among these cultivars. Especially, Jangdae cultivar contained the highest amount of vitamin E and vitamin K. Thus, these data suggested that Jangdae and Auxu is the most desirable cultivar containing high amount of vitamin and amino acid.

Anthocyanin, a group of purple or reddish flavonoids, have been recognized as health-promoting functional food ingredients due to antioxidant activity. For this reason, plant breeders are trying to increase the anthocyanin contents using methods such as classical breeding and biotechnological approaches. To broaden the mutants population, seeds of colored wheat variety (K4191) were irradiated by using 250 Gy gamma irradiation. Individual 968 M4 plants were grown in Korea Atomic Energy Research Institute field. Many mutant phenotypes were shown: seed color variation, abnormal spike shape, awning formation, heading and ripening time, plant height, ripening period, super dwarf, etc. To identify the inheritance traits of colored-wheat, individual lines were maintained the spike base classified by generation. Characteristics per spike and plant were piled up to construct for mutant database. In the future, fixed descent will be analyzed the anthocyanin contents or other phytonutrients by ultra-performance liquid chromatography (UPLC). Expression of seed color-related transcription factors and anthocyanin biosynthetic pathway genes will be examined.

Gamma irradiation has been used as a tool for plant mutation breeding to select new cultivar with improved characteristics. Generally, the irradiation of seeds with high doses of gamma rays disturbs the synthesis of protein, hormone balance, and enzyme activity. And also, high dose of gamma rays to reduce plant height, number of tiller, and root length, although the effect of gamma-irradiated plants may depend on the species and cultivar or stress conditions. Biological effects of radiations can be divided into two types according to dose range and periods of exposure. Acute irradiation represents exposure to high-dose of irradiation over short period time, whereas the chronic irradiation is comprised of exposure to low doses of radiation over extended period of time. To compare the effects of acute and chronic exposure to ionizing radiation on two wheat cultivars (K4191 and Geumgangmil), we measured their germination rate, seedling height, and root length. In order to understand the influence of antioxidant-related genes and DNA repair-related genes, we used qRT-PCR methods to identify their expression levels. To study the behavior of a radiation-induced free radical, gamma-irradiated seeds were used for ESR spectroscopy. Plant growth pattern was showed positive correlation with ESR results. This study indicates that low level chronic radiation exposure is even more serious effects than short doses of high level radiation according to different wheat cultivar.

Chronic gamma irradiation can be used an alternative mutation breeding methods for induction of many useful mutants. Seedlings of purple-colored wheat plants were irradiated with wide range doses of chronic gamma-rays (20, 25, 30, 40, 50, 70, 100, 125, 150, 200, 250, 300 Gy) during 6 weeks at gamma-phytotron in the Korea Atomic Energy Research Institute, respectively. To identify the biological responses purple-colored wheat, we examined the plant height, chlorophyll, carotenoid and total anthocyanin contents in leaf. Plant growth, chlorophyll and carotenoid contents in leaf were decreased when the dose rate increased. Anthocyanin contents were increased with the increase of the radiation dose until 50 Gy treatment. To confirm the real contents of anthocyanin, we also investigated cyanidin-3-glucoside in purple-colored wheat leaf by using UPLC analysis. These results indicate that anthocyanin accumuation was observed under chronic gamma irradiation.