The system of rice intensification (SRI) has been proposed as a way of transitioning towards more sustainable rice farming. In rainfed rice farming, however, various environmental constraints often make SRI practices challenging to adopt. We conducted an on-farm experiment in 30 rainfed lowland fields to examine the technical efficacy of SRI in southern Cambodia during the wet seasons of over three years of 2012, 2013, and 2015. Across the three years, the SRI practices produced significantly greater plant biomass and grain yield than non-SRI. We ascribed the yield increase to the increased number of grains per land area as the increased number of spikelets per panicle rather than the number of panicles per land area. With no significant difference in seedling age between the SRI and the non-SRI, we attributed the more substantial number of grains per panicle to the reduced planting density, increased manure application, and reduced amount of inorganic fertilizers in SRI fields. Our results suggest that the advantage of the SRI lies in the efficient use of internally available soil nutrients, whose mechanisms need quantitative understanding through future studies.

작물 생육 진단에 있어서 군락 엽면적과 군락 피복은 주한 요소 이다. 최근에는 이러한 측정을 디지털 카메라를 활용하여 RGB 식생지수로 작물 생육을 측정하고 있다. 본 연구는 밀 재배 기간 카메라의 노출 값을 다르게 설정하여 RGB 컬러 식생 지수와 군락 엽면적지수 및 군락피복에 대해 평가하였다. 군락 엽면적, 군락 피복 및 디지털 영상 측정은 출수 16일전부터 출수 후 25일까지 하였다. 일출 후 30분 이내에 촬영하였다. 노출 값은 셔터 속도를3 수준(1/60s, 1/340s, 1/640s)으로 하였다. RGB 컬러 식생지수 분석은 파이썬으로 하였다. 실제 군락 엽면적과 군락 피복간에는 정의 상관관계(r2 =0.94)를 보였다. 군락 피복 이미지의 ExG, GLI, NGRDI, ExG-ExR, MExG, TGI, MNGRD 및 MExG-CIVE는 군락 엽면적 지수와 및 군락 피복과는 정의 상관관계를 보였다. 그러나 CIVE와 ExR은 부의 상관관계를 보였다. 본 연구 결과 1/640s 가 1/60s와 1/320s에 비하여 노출이 높게 설정된 것으로 보였다. 또한, 토양과 녹색 영역을 분리하는데 있어서 너무 어린 시기 보다는 출수 전이 가장 잘 분리되었다. 따라서 야외에서의 다양한 광 조건에서는 과도한 노출보다는 광 간섭을 줄이는 기술과 함께 작물의 생육 시기와 기상환경을 고려하여 노출 값이 설정되어야 할 것이다.

The purpose of this study was to evaluate the forage maize varieties and sowing date under wet seasons conditions and determined the effects on yield in Cambodia. In this study, the number of days from sowing to silking was approximately nine longer in May and June than in July, August, and September. The number of days from silking to harvest was seven shorter in May and June than in July, August, and September. The variety with the least decrease in ear height was ‘BT5666’, and the variety with the least decrease in ear height ration was ‘CP888’. On the other hand, in sowing days, ear height ratio decreased in September. The best sowing season in ear length, ear width, number of ears and number of grains per row was in May. In this study, ‘BT5666’ was the variety with significant decrease with a 90% reduction in yield. The results indicate that the yield was high during the sowing period in May and in June because of the appropriate precipitation, temperature, and solar radiation. However, August and September had significantly reduce in yield by high precipitation and low solar radiation.

This study was conducted at the National Institute of Animal Science (NIAS) from 2010 to 2017 to develop a late-maturing variety with high productivity for cultivation in the southern region of South Korea. The new variety of Italian ryegrass, ‘IR901’, was a late-maturing variety, and its heading date was 22 May, 17 days later than that of the control variety ‘Kowinearly’. ‘IR901’ had a flag leaf width of 11.2 mm, flag leaf length of 31.8 cm, and plant length of 103 cm on its heading date. The combined average dry matter yield of ‘IR901’ in all three adaptability evaluation regions (Cheonan, Pyeongchang and Haenam) was 7,747 kg/ha, which was similar to that of the ‘Kowinearly’ variety (7,734 kg/ ha). However, the average dry matter yield over three years in Cheonan and Pyeongchang was 82% and 96%, respectively, compared to that of the control, which was most likely because of the poor cold tolerance of ‘IR901’. By contrast, in Haenam, in the southern region, the average dry matter yield of ‘IR901’ was 19% higher than that of the ‘Kowinearly’ variety. The proportions of crude protein (CP), total digestible nutrients (TDN), acid detergent fiber (ADF), and neutral detergent fiber (NDF) in ‘IR901’ were 8.6%, 59.7%, 36.9%, and 54.8%, respectively; the proportions were 0.2% lower, the same, the same, and 2.5% lower than those in the ‘Kowinearly’ variety. The determined in vitro dry matter digestibility (IVDMD) of ‘IR901’ was 72.2% higher than that of ‘Kowinearly’ (67.2). In general, of the two varieties, the forage quality of ‘IR901’ was marginally superior to that of ‘Kowinearly’.

본 연구는 이탈리안 라이그라스를 벼 입모중 파종 후 벼 수확시기에 따른 이탈리안 라이그라스 극조생종 품종 ‘그린팜’ 의 생육특성 및 생산성을 평가하기 위해 중부지역에 위치한 천안에서 2011–2012, 2012–2013 및 2013–2014까지 3년간 수행하였다. 1. 국내육성 이탈리안 라이그라스 극조생종 ‘그린팜’을 3년 간 9월 하순에 파종 후 10일(처리 1), 20일(처리2) 및 30일(처리 3) 간격으로 3회에 나누어 벼를 수확해서 재배하였을 때, 벼 수확시기가 늦어질수록 월동 전 분얼수, 초장, 월동율 및 건물수량은 감소하는 것으로 나타났다. 2. 월동 전 분얼수는 처리 1에 비해 처리 2 및 3의 평균이 각각 32% 및 52% 감소하는 것으로 나타났다. 3. 벼 수확 지연에 따른 월동율의 경우 처리 1에 비해 처리 2 및 3은 두 처리구 모두 평균 8% 감소하였으며, 건물수량은 각각 평균 32% 및 52% 감소하였다. 4. 벼 입모중 이탈리안 라이그라스 파종 재배시험에서 출수기는 매년 다르게 나타났으며, 봄철 이탈리안 라이그라스 수확 시 초장의 차이는 유의성이 있는 차이를 보이지 않았다. 5. 논에서 입모중 파종으로 극종생 이탈리안 라이그라스 품종 그린팜을 재배할 때는 파종 후 가능한 한 빨리 벼를 수확하여 생산성을 높이는 것이 방법임을 시사한다.

This study was conducted at the National Institute of Animal Science from 2010 to 2017. As a variety that is sufficiently productive in the southern regions to replace imported varieties and sufficiently cold-resistant to be cultivated in the central-northern regions, "IR605" was developed and submitted to the Korea Seed & Variety Service in an application for protection. The novel Italian ryegrass variety "IR605" is a diploid with green leaves, a semi-erect growth habit before wintering, and an erect growth habit in the spring. "IR605" was a medium maturing variety with a heading date of around May 15th. "IR605" had a flag leaf width of 9.9 mm, flag leaf length of 26.7 cm, and plant length on the heading date of 100 cm, which was approximately 5 cm longer than "Kowinearly." The stem thickness and ear length of "IR605" are 0.08 mm thicker and 0.5 cm longer than those of "Kowinearly", respectively. The cold-resistance of "IR605" was weaker than that of "Kowinearly", but strong enough to be cultivated in Pyeongchang, Gangwon province. The dry matter yield of "IR605" (9,308 kg/hectare) was 20% higher than that of "Kowinearly", which was further pronounced in the southern region of Haenam, where there was a 52% increased (p < 0.05). The in vitro dry matter digestibility of "IR605" was 68.4% at which was slightly higher than that of "Kowinearly", The total digestible nutrients was 58.5%, which was slightly lower than "Kowinearly". Overall, the feed quality characteristics of "IR605" were similar to those of "Kowinearly".

This study was carried out to introduce of agronomic characteristics, forage yields and quality of Sorghum × Sudangrass hybrids ‘Cadan 99B’ and ‘Sweet Sioux WMR’ from 2015 to 2016 in middle and southern regions of Korea. The field experiment design was complete in seven varieties with three repetitions. Sorghum × Sudangrass hybrids were sown on mid-May in middle region, and end-May in southern region of Korea, in 2015 and 2016. The observed average heading date of Cadan 99B and Sweet Sioux WMR were July 22. The heading dates of Cadan 99B and Sweet Sioux WMR were 8 days earlier than SX-17 and 5 days earlier than brown mid-rid (BMR) Revolution. The sugar contents of Cadan 99B and Sweet Sioux WMR were 6.5 and 6.9 Brix°, respectively. Comparison with BMR variety, the sugar contents of Candan 99B and Sweet Sioux WMR were 0.2 and 0.6 Brix° higher than Revolution, respectively. The average of dry matter (DM) yield for 2 years and 2 regions of Cadan 99B (24,587kg/ha) were the highest among the seven varieties, but there was no significant difference among other varieties except headless control variety Jumbo (19,119kg/ha) and LATTE (20,778kg/ha) (p>0.05). The crude protein (CP) and in vitro dry matter digestibility (IVDMD) of Cadan 99B were 7.5% and 60.2%, and Sweet Sioux WMR were 6.9% and 60.7%, respectively. The results of this study indicated that Sorghum × Sudangrass hybrids Cadan 99B and Sweet Sioux WMR are earlier heading dates and higher than SX-17, and high yields of DM in middle and southern regions of Korea.

This experiment was carried out to compare the growth characteristics and productivity between sudangrass and sorghum-sudangrass hybrid under the same cultivation condition in Cheonan and Kimje of Korea from 2010 to 2011. Sudangrass hybrids(Choice, Sunstar and Piper) and sorghum-sudangrass hybrids(SX-17, Jumbo and Revolution) were planted 5th May in Cheonan and 7th May in Kimje. Heading date of sudangrass hybrids was 12th July which was about 2 week fast more than that of sorghum-sudangrass hybrids. Stem diameter of sudangrass hybrids was about 6mm which was about 5 mm smaller than that of sorghum-sudangrass hybrids. Disease resistance of sudangrass hybrid, “Choice” was similar to that of sorghum-sudangrass hybrids. Dry matter yield of “Choice” was 15ton/ha which was 10% less than that for sorghum-sudangrass hybrids(SX-17). Feed value of “Choice” was similar or slightly lower than that of sorghum-sudangrass. For increasing the productivity in forage cropping systems all around year, sudangrass hybrid is recommended for prolong by the middle ten day of May in the harvesting time of italian ryegrass

This experiment was carried out to investigate the effect of harvesting time of Italian ryegrass (Lolium multiflorum Lam; IRG) in spring on dry matter (DM) yield. IRG cultivars ‘Kowinearly’ and ‘Greenfarm’ were seeded at 50 kg/ha and grown on rice paddy fields. There was no difference in growth characteristics between both varieties before winter. However, cold tolerance of Kowinearly was higher than that of Greenfarm, as demonstrated by post-wintering growth characteristics. The heading date of both varieties was affected by the spring weather. The IRG was harvested three times at 5-day intervals beginning from 9 May. The DM yield of Greenfarm was 6,306; 7,335; and 8,109 kg/ha, and that of Kowinearly was 7,498; 9,196; and 10,449 kg/ha at the three consecutive harvests. The delay of the harvesting time for 5 and 10 days increased the DM yield of Greenfarm by 16% and 29% and that of Kowinearly by 23% and 39%, respectively, compared to the yield at first harvest (p < 0.05). Therefore, IRG harvest later than early to mid-May is expected to increase productivity. The feed values of Greenfarm were: 12.2% of crude protein (CP), 34.5% of acid detergent fiber (ADF), 57.7% of neutral detergent fiber (NDF), 61.6% of total digestible nutrients (TDN), and 72.3% of in vitro DM digestibility (IVDMD). For Kowinearly, these values were 16.4% of CP, 30.4% of ADF, 52.7% of NDF, 64.9% of TDN and 79.0% of IVDMD.

The present study investigated the effects of different seeding rates on growth characteristics and seed productivity of the “Kowinearly” cultivar of Italian ryegrass (Lolium multiflorum Lam.) in paddy fields. Sowing Kowinearly in paddy fields under growing rice at the rates of 20, 30, 40, and 50 kg/ha resulted in seed yields of 1.57, 1.92, 2.06, and 2.09 ton/ha, respectively. Seed yield of Kowinearly was the highest at a seeding rate of 50 kg/ha (p<0.05), at which the cultivar was able to survive in winter and the weed ratio was low. Under these conditions, most growth characteristics such as winter survival (85%), weed ratio (10%), stems per square meter (1,006) were superior than those sown at other seeding rates (p<0.05). In addition, it has been reported that the economic efficiency of the 50 kg/ha seeding rate was higher than that of the other seeding rates.

The objective of this study was to determine the effect of harvesting time of rice on growth characteristics and dry matter yields of Italian ryegrass (Lolium multiflorum Lam., IRG) ‘Kowinearly’ after seeding them in paddy field. Experiments were performed in National Institute of Animal Science, RDA (Cheonan, Choongcheongnam-do province of Korea) for four years (2011~2014). Italian ryegrass variety ‘Kowinearly’ was seeded at experimental paddy fields before rice harvest in late September. Rice were harvested at 10, 20, and 30 days after seeding of IRG. As the harvest time of rice became later, the number of tillering before winter was reduced and IRG plant length before winter was shorter. The dry matter yield of IRG was also reduced. When rice were harvested at 20 days and 30 days after sowing of IRG compared to the rice harvested in autumn at 10 days after sowing of IRG, the number of tillering for IRG before winter was decreased by an average of 19% and 40%, respectively. The winter survival ratio of IRG was decreased by an average of 13% and 19%, respectively. Their dry matter yields were also reduced by an average of 8% and 19%, respectively. The heading date was different from each other in three years. The plant length at the time of IRG harvest in the spring was not significantly different among treatment groups. Taken together, our results suggest that when Italian ryegrass is grown in paddy fields by the method of ‘seeding of IRG before rice harvest’, rice should be harvested as soon as possible after sowing of IRG ‘Kowinearly’ in the autumn to increase forage productivity.

This study was conducted to evaluate the growth characteristics and dry matter yields of Italian ryegrass (Lolium multiflorum Lam., IRG) cultivars for three years (2011~2013). The cold-resistance of domestic cultivars was stronger than other foreign varieties. Among early-maturing cultivars, dry matter (DM) yield of ‘Kowinearly’ was significantly higher (8,751 kg/ha) than other cultivars (p<0.05). In medium-maturing cultivars, DM yield of ‘Kowinmaster’ and ‘Tam 90’ was 9,394 and 7,461 kg/ha, respectively. Of the two, the former’s DM yield was higher than the latter’s (p<0.05). Among late-maturing cultivars, DM yield of ‘Hwasan 104’ (9,809 kg/ha) was similar to ‘Hwasan 101’ (9,354 kg/ha), but significantly higher than the other late-maturing cultivars (p<0.05). According to the results obtained from present study, early-maturing cultivars with cold-tolerance and high productivity is required for the paddy field cultivation of an Italian ryegrass cultivar after rice harvest in Cheonan region. Medium- and late-maturing cultivars are more advantageous for the dry field cultivation of the maximum quantity that is possible. In conclusion, to select which cultivar/s to grow, the climate of the growing area, weather conditions, and the cropping system must all be considered

Nitrogen (N) fertilizer management is one of the important aspects of economic production of sorghums in sustainable agriculture. The aim of the study was to evaluate the effects of different N application rates and its split N application methods on productivity, growth characteristics, N accumulation, N use efficiency (NUE), and feed value of Sorghum × Sudangrass hybrids. Treatments consisted of five N application rates (0, 150, 200, 250, and 300 kg ha-1) and two split N application methods (40% in basal N, 30% at the growing stage, and 30% after the first harvest vs. 50% in basal N and 50% after the first harvest). Plant height, leaf width, and stem diameter were increased (p≤0.05) with increasing N fertility rates at each harvest. Chlorophyll content (expressed as SPAD values) was the highest at a rate of 300 kg N ha 1 (first harvest, 46.32; second harvest, 33.09). It was the lowest at zero N (first harvest, 21.56; second harvest, 18.5). Total N, N uptake, and NUE were increased with higher N rates. Split N application had little effect on total N, amount of N uptake, or NUE. Total dry matter yields were the highest (21,715 kg ha-1) at a rate of 300 kg N ha-1. It was the lowest (10,054 kg ha-1) at zero N. Our results suggest that more than 300 kg N ha-1 can improve dry matter yield to be above 116% compared to zero N, thus enhancing the agronomic characters of sorghums. However, no significant effect had been found for split N application. Further work is needed to determine the optimal N levels and the effect of split N application rates.

This study was carried out to determine the effect of mixed pasture using domestic varieties orchardgrass ‘Kodione’ and tall fescue ‘Purumi’ on forage yields and botanical composition from 2013 to 2015 in middle region of Korea. The following mixed pastures were used in this study: treatment A (orchardgrass oriented mixture with imported varieties), treatment B (orchardgrass oriented mixture), treatment C (orchardgrass and tall fescue oriented mixture), treatment D (tall fescue oriented mixture), treatment E (tall fescue oriented mixture without orchardgrass), treatment F (only orchardgrass), and treatment G (only tall fescue). In botanical compositions at last cutting time in 2015, treatment A had orchardgrass at 7.1%, tall fescue at 47.9%, kentucky bluegrass at 15.2% and white clover at 25%. Meanwhile, treatment B had orchardgrass at 25.9%, tall fescue at 47.0%, kentucky bluegrass at 4.9% and white clover at 18.3%. Consequentially, in botanical composition from 2014 to 2015, treatment A was changed more compare to other treatments B, C, D and E. The average of dry matter (DM) yield for 2 years of treatment E (18,369 kg/ha) was the highest among the seven treatments, but there was no significant difference among other treatments except treatment A (p>0.05). The crude protein (CP) and in vitro dry matter digestibility (IVDMD) of treatment A were 14.5% and 74.8%, and treatment E were 14.1% and 73.0%, respectively. The content of neutral detergent fiber (NDF) and acid detergent fiber (ADF) were lower in treatment E and A than other treatments, and treatment E showed high content of total digestible nutrient (TDN). Therefore, tall fescue oriented mixture using domestic tall fescue variety Purumi had good forage productivity, quality and botanical composition in middle region of Korea for the establishment of grassland.

본 연구는 2014 / 2015년 동계 사료작물의 월동 후 생육 조사를 통하여 전국적인 작황을 파악함으로서 사료작물 생 육에 문제점이 있을시 근본 원인을 구명할 수 있는 기초자 료로 이용하거나 조사료의 연중 안정 생산, 공급에 도움을 주고자 수행하였다. 2015년도 전국 동계 사료작물의 월동 후 생육 상황은 전반적으로 저조하였는데, 월동률 분포에 따른 지역별 분포 상황을 살펴보면 월동률 80% 이상인 지 역이 66%, 월동률 79~50% 분포지역이 24.9%, 50%미만 지 역이 9.1%의 지역별 분포를 나타내어 전체적으로는 79%의 월동률을 나타냈다. 월동 후 월동률 및 피복률은 배수로가 설치된 논에서는 각각 83%와 80%로서 양호했으나 배수로 가 설치 안 된 습한 논에서는 각각 67%와 66%로 낮은 경 향을 나타냈다. 전국 동계작물 조사료 생산량은 강원, 충 북, 충남, 경남, 전남지역은 10~15%, 경기, 경북, 전북지역 은 약 30%의 수량감소가 예상되어 전국적으로 약 19%의 수량 감소가 예상되었다.

본 시험은 새로운 여름 사료작물인 케나프(kenaf; Hibiscus dannabinus L.)의 생육특성, 수량성 및 품질을 다른 사료작물과 비교할 목적으로 2013년부터 2014년까지 2년간에 걸쳐 축산과학원 축산자원개발부 초지사료과 내의 시험포장에서 수행하였다. 중부지역에서 케나프(홍마 300), 옥수수(광평옥) 및 수수×수단그라스(점보)의 생육특성 및 품질을 비교한 결과를 요약하면 다음과 같다. 당도는 홍마 300이 5.4 Brix°로 광평옥보다 1Brix°, 점보보다 2.9 Brix° 높았다. 출현율과 도복은 세 작물 모두 각각 80% 이상, 1.5 이상으로 양호하게 나타났다. 생초수량은 점보가 홍마 300보다 12% 높았으며, 건물중은 홍마 300이 제 작물 중 유의성 있게 가장 높게 나타났다(p<0.05). 하지만, TDN 수량은 홍마 300이 광평옥보다 13% 유의성 있게 낮았다(p<0.05). ADF 및 NDF 함량은 홍마 300이 57.8%, 69.5%로 가장 높았으며 TDN 함량 및 건물소화율은 43.3% 및 44.4%로 가장 낮았다. 따라서 본 연구결과를 토대로 볼 때 케나프인 홍마 300은 건물수량은 높지만 사료 품질에서 광평옥 및 점보보다 많이 낮았다. 그 결과 TDN 수량성은 광평옥보다 13% 낮은 결과를 얻었다. 이상의 결과를 종합하여 볼 때, 새로운 사료작물인 케나프는 단위면적당 생초 및 건물수확량은 옥수수보다 높지만, 건물소화율 및 TDN 함량이 낮아 TDN 수량은 옥수수보다 낮은 것으로 나타났다. 결과적으로 홍마 300은 높은 수량성을 가지고 있지만 낮은 사료가치를 가지고 있는 것으로 나타났다.

본 시험은 2011년부터 2013년까지 3년 동안 경북 예천 군 농가포장에서 이탈리안 라이그라스(Lolium multiflorum Lam) 국내육성 품종과 수입품종의 생육특성 및 수량성을 평가하기 위하여 실시하였다. 시험설계는 품종을 처리로 해서 조생종 6품종, 중생종 2품종, 만생종 4품종 등 모두 12처리, 난괴법 3반복으로 배치하였다. 파종은 2011년에는 9월 26일, 2012년에는 9월 25일에 실시했으며, 수확은 품 종별로 출수기에 맞추어 수확하였다. 대부분의 국내육성 품종이 도입품종에 비해 내한성이 더 강한 것으로 나타났 다. ha당 건물수량은 조생종에서는 ‘코윈어리’ 품종이 10,031 kg으로 다른 5품종보다 많았으며(p<0.05), 중만생종 에서는 ‘화산 104호’ 품종이 ‘화산 101호’ 및 ‘코윈마스터’ 품종과 비슷하였으며 다른 품종보다 건물수량이 많았다 (p<0.05). 본 연구의 결과에 의하면 중부지역에서 답리작 재배 시 ‘코윈어리’ 또는 국내육성 조생종 품종을 선택하 는 것이 유리하며, 논이 아닌 밭에서는 중만생종이 유리할 수 있을 것으로 판단된다. 따라서 품종을 선택할 때에는 재배지역의 겨울철 기온, 강수량 및 작부체계를 고려하는 것이 중요하다고 사료된다.

This study was investigated the effects of various commercial sanitizers on microbial characteristics in fresh-cut iceberg lettuce during storage. For screening sanitizer, lettuce was cut and dipped in chlorine water (0.2 ml·L-1), solution of organic acids such as ascorbic acid, citric acid, acetic acid, mixture of ascorbic acid and acetic acid (1-6%), and solutions of commercial sanitizers such as Formula 4TM (1,3,4%), Fresh produce washTM (1,3,4%), CleancolTM (1%), ChitocholTM (1%) and Natural CaTM (0.1%) for 3 min, respectively. Washing lettuce with selected sanitizers resulted in reduction of aerobic bacteria of more than 2 log CFU/g. Initial pH of lettuce was related with the pH of sanitizers. pH ranged from 4.7 to 6.1 in Formula 4 (4%, pH 1.7) and Natural Ca (0.1%, pH 12.0), respectively. Chlorine water showed consistent and significant inhibition effect in all of microorganisms except total coliform. Over 3% of Formula 4 and Fresh produce wash were found to have high bactericidal activity among sanitizers. The sanitizers of chlorine water, Fresh produce wash, Chitochol and Natural Ca were effective in reducing yeast and mould populations. As coliform and E. coli, Formula 4 (4%) showed the highest bactericidal activity. The bactericidal effect of commercial sanitizers during storage varied with the kinds and concentrations of tested sanitizers. Although inhibition effect was not showed during storage, these results suggest that commercial sanitizers could be an alternative to chlorine for washing fresh-cut produce.

Knowledge of the chromosomal constitution of the ancestors of modern soybean will complement plant breeding efforts to improve agronomic and economic characteristics of soybean. Variation block (VB)-based comparison using genome-wide insertion/deletion (InDel) markers was used on a diverse panel of 147 soybean cultivars to assess the impact of chromosomal changes during modern breeding. There were identical variation patterns of the examined InDels consistently appearing in the genome parts arising from parental varieties, indicating that soybean chromosomes in descendants should be all determined by genetic reshuffling of VBs inherited from parental chromosomes. Structure analysis of the accessions through the 202 InDels separated the accessions into four subgroups. Gene introgression revealed by the structure analysis agreed with the fact that a limited number of landraces and elite varieties were introduced and used as donors for breeding soybean cultivars in pedigree analysis. Especially, VBs became more reshuffled over time as a result of the breeding process, which resulted in using breeding parents with new VB-types for improving the end-use value of soybean. Therefore, their clustering using the 202 VB-specific InDels is strongly influenced by the difference in breeding ancestors among the subgroups. This indicates that the 202 InDel markers are very useful for genetic study by analyzing the reshuffling patterns of the parental genomes in the descendant.

본 연구에서는 새로운 프로바이오틱스를 선발하고자 protease를 생산하는 GRAS균주를 된장 및 청국장으로부터 분리하고 그 특성을 조사하였다. 분리한 균주를 분리 동정 한 결과 각각B. amyloliquefaciens CDD5, B. amyloliquefaciens CPD4 및B. amyloliquefaciens CGD3로 명명하였다. 분리균 주의 생육은 12시간 배양 시, 7.13~7.32 log CFU/mL로 최대 생육도를 보였으며 24시간까지 유지되다가 감소하는 경향 을 나타내었고, 그 중B. amyloliquefaciens CGD3의 protease 활성이9.21 U/mL로서가장높게나타났다. B. amyloliquefaciens 이 생산하는 protease 활성은 pH 7.0~10.0, 온도는 50℃에서 최적활성을 나타내었으며, casein에서 protease활성이 가장 우수하게 나타났다. pH, NaCl, glucose 농도를 달리한 배양 조건에 따른 B. amyloliquefaciens의 생육특성으로는 pH 5.0~10.0의 넓은 범위에서 생육하였고, NaCl은 12% 농도까 지 증식하였으며 glucose 5%까지는 생육하나 10% 농도부 터는 현저하게 생육이 낮아짐을 확인하였다. 또한 혈당저 하를 유도하는 α-glucosidase의 저해활성을 측정한 결과, B. amyloliquefaciens CPD4 및B. amyloliquefaciens CGD3에 서는 각각 96.65% 및 97.85%의 저해율을 나타내었다. 이러 한 결과는 특히 protease활성이 가장 우수하고 α-glucosidase 또한 높은 저해활성을 가진 B. amyloliquefaciens CGD3 균 주의 생육특성을 고려하여 단백질 분해 분야의 프로바이오 틱 적용 및 당뇨예방용 기능성식품 소재로의 가능성이 기대 된다.