Black phosphorus (BP) is incorporated in the electrochemical detection of uric acid (UA) to form few layers of BP nanosheets (BPNS)-modified glassy carbon electrodes (BPNS/GCE), investigated by means of ultrasound-assisted liquid-phase exfoliation. We find a significant increase in the peak current magnitude and positive potential shift in the electrochemical response of BPNS/GCE, which may be attributed to the larger specific surface area and good charge transfer ability of BPNS. Further, the electrochemical response of BPNS/GCE is evaluated under different conditions to achieve the optimal conditions. UA detection using differential pulse voltammetry (DPV) shows linear response within the range of 1–1000 μM with a detection limit of 0.33 μM. This work reveals new applications of BP nanomaterials in the electrochemical sensing, thereby promoting further advancement in terms of practical applications of two-dimensional nanomaterials.

The progress of science and technology has driven the development of the society, and the application of threedimensional simulation technology in all walks of life has greatly improved people's life. Maritime affairs is an important part of comprehensive transportation. The improvement of maritime supervision and service level is conducive to the construction of domestic first-class economic law enforcement system and international leading maritime management institutions. Now the application of 3D simulation technology to maritime supervision is studied, aiming to improve the quality and efficiency of supervision, make maritime supervision more convenient and have further guarantee. Maritime supervision of ship environment is often more troublesome than personnel management. In the process of monitoring and control, many uncontrollable environmental factors greatly increase the difficulty of supervision, among which the port environment is more complex, which is a difficulty of monitoring and control in maritime supervision. This paper uses MapInfo Professional, Win Topo, Multigen Creator and other operating software to construct the Qingdao port visual simulation environment. The port topographic cloud map of Qingdao Port was captured, and the collected image information was converted into data through MapInfo Professional and Win Topo software, and then modeled by Multigen Creator software. After completing the general terrain outline, the established simulation scene was made more realistic and realistic through some detail processing and optimization means. By describing the establishment of 3D simulation terrain in Qingdao Port, the application status of the simulation technology in ship environment and port management is reflected, which provides a reference for the deeper application of 3D simulation technology in maritime supervision.

Transition-metal-embedded carbon nanotubes (CNTs) have been accepted as a novel type of sensing material due to the combined advantage of the transition metal, which possesses good catalytic behavior for gas interaction, and CNTs, with large effective surface areas that present good adsorption ability towards gas molecules. In this work, we simulate the adsorption of O2 and O3 onto Rh-doped CNT in an effort to understand the adsorbing behavior of such a surface. Results indicate that the proposed material presents good adsorbing ability and capacities for these two gases, especially O3 molecules, as a result of the relatively large conductivity changes. The frontier molecular orbital theory reveals that the conductivity of Rh-CNT would undergo a decrease after the adsorption of two such oxidizing gases due to the lower electron activity and density of this media. Our calculations are meaningful as they can supply experimentalists with potential sensing material prospects with which to exploit chemical sensors.

Customers are regarded as the key intangible assets of a company. It is necessary to have the capability to anticipate customer value. The study discusses the relationship among customer value anticipation, product innovativeness, and customer lifetime value from customer perspective. 178 MBA students were surveyed by questionnaire in this study. The results show that customer perceived customer value anticipation can significantly influence product innovativeness. Product innovativeness not only positively affects customer lifetime value, but also plays a partial mediating role between customer value anticipation and customer lifetime value. Both functional and emotional advertising can play a moderating role on the relationship between product innovativeness and customer lifetime value. At the end, managerial implications are discussed.

In recent years, electronic products have played an increasingly important role in modern society and have penetrated into people’s life and work. With the continuous development of technology and the increasing demand for convenience and innovation, the functions and performance of electronic products continue to improve. However, the intensification of market competition and the rapid advancement of technological progress have brought many challenges and opportunities to the field of electronic products. This paper takes the new product development project management process of Lenovo Company as the research object, adopts investigation analysis method, literature analysis method and project management theory and gateway development management theory to deeply analyze the existing problems in the new product development project management of Lenovo Company, such as: The new product development cycle is long, the new product development cost is over budget, the new product development project organization and coordination ability is weak, and the project monitoring and feedback are not timely. The causes of these problems are analyzed. In view of the existing problems, this paper makes an in-depth study of the relevant theories of project management and new product development, and puts forward an optimization scheme suitable for the improvement of the new product development project management of Lenovo Company. It means adopting agile development scheme, establishing project development cost control point, optimizing organizational structure, and establishing perfect project management supervision and feedback mechanism. Finally, based on the current situation of Lenovo ‘s new product development, this paper proposes the guarantee measures for the smooth implementation of the new product development project management optimization plan, namely, talent guarantee, development and innovation awareness, risk control awareness, and management awareness in strengthening cultural construction, so as to ensure the smooth implementation of new product development project management.

Culture, as the sum of human knowledge, beliefs, ethics, laws, customs, etc., is always affecting the way people think, talk, live and work, and affect people's thinking and behavior. Different nationalities and regions determine that people in various countries and regions have different cultures, and even a country and a region have multiple cultures. Modern sports have been operating in a globalized economy. In the past ten years, the trend of international sports development has become more obvious. Sports has promoted the process of globalization by developing a global mass consumer culture. In the field of sports management, cross-cultural issues also arise, and the collision of different cultures is always of great significance to how to manage sports organizations. This article analyzes the cultural differences in the process of cultural and intercultural sports management, explores the issues of cultural conflict and cultural integration encountered in sports management, and combines sports management with crosscultural management strategies in the field of international commerce Corresponding approaches are put forwar.

The urban wetland park is an indispensable part of an ecological city. It has aroused widespread attention and even quickly planning and construction around the country. But, in terms of the nature of the urban wetland park, there is a contradiction between the protection of urban wetland resources and the openness of the urban park for public. This article starts with the necessity for construction of urban wetland park, more over expound the existent problems between the protection and openness of the urban wetland park and to propose appropriate measures through the case study on Nanhu National Urban Wetland Park, Tangshan.

An efficient somatic embryogenesis and plant regeneration protocol was developed for Schisandra chinensis Baill, using embryogenic cell suspensions and optimized media conditions. Friable embryogenic callus was induced from cotyledonary leaf and hypocotyl explants of 7 days old seedlings on MS agar medium supplemented with 1.0 to 4.0 mg l-1 of 2,4-dichlorophenoxyacetic acid (2,4-D). Fast growing and well dispersed embryogenic cell suspensions were developed within two months when embryogenic calli were transferred to MS liquid medium containing 1.0 mg l-1 2,4-D. One third strength of MS medium was the best for both overall growth and development of somatic embryos in liquid culture. Over 3400 viable somatic embryos were produced from each 150 ml flask with an initial cell density of 30 mg in 30 ml medium. Germinated somatic embryos developed in liquid medium converted into plantlets after transferred to half-strength MS semi-solid medium. Approximately 90% of the converted plantlets were successfully transplanted to soil and grew into fertile plants.

Field performance and morphological characterization was conducted on seven transgenic lines of Codonopsis lanceolata expressing γ-TMT gene. The shoots were obtained from leaf explants after co-cultivation with Agrobacterium tume-faciens strain LBA 4404 harboring a binary vector pYBI 121 that carried genes encoding γ-Tocopherol methyltransferase gene (γ-TMT) and a neomycin phosphotransferase II gene (npt II) for kanamycin resistance. The transgenic plants were transferred to a green house for acclimation. Integration of T-DNA into the T0 and T1 generation of transgenic Codonopsis lanceolata genome was confirmed by the polymerase chain reaction and southern blot analysis. The progenies of transgenic plants showed phenotypic differences within the different lines and with relative to control plants. When grown in field, the transgenic plants in general exhibited increased fertility, significant improvement in the shoot weight, root weight, shoot height and rachis length with relation to the control plants. However, all seven independently derived transgenic lines produced normal flower with respect to its shape, size, color and seeds number at its maturity. Indicating that the addition of a selectable marker gene in the plant genome does not effect on seed germination and agronomic performance of transgenic Codonopsis lanceolata. T1 progenies of these plants were obtained and evaluated together with control plant in a field experiment. Overall, the agronomic performance of T1 progenies of transgenic Codonopsis lanceolata showed superior to that of the seed derived non-transgenic plant. In this study, we report on the morphological variation and agronomic performance of transgenic Codonopsis lanceolata developed by Agrobacterium transformation.

An efficient regeneration system was developed using leaf, petiole, and internode explants. Highly embryogenic callus was obtained following cultivation on MS basal nutrient supplemented with 2 mg/l 2,4-D. Globular, heart, torpedo and cotyledon shaped somatic embryo were produced from the surface of embryogenic callus. Direct shoot regeneration without intermediate callus formation has been achieved on MS medium supplemented NAA and BAP. The percentage of response varies with different concentration of auxin and cytokinin treated individually or in combination. The best shoot regeneration response (54.28%) and number of shoot per explant (12.67) were achieved on the medium supplemented with 0.1 mg/l NAA and 1 mg/l BAP. The regenerated shoot transformed into young plant when cultured into elongation and root induction medium. More than 90% of in vitro propagated plants could survive when transferred to the greenhouse for acclimation. This optimized regeneration system can be used for rapid shoot proliferation and genetic transformation.

Acifluorfen tolerance charateristics determined the involvement of absorption, translocation, and metabolism in acifluorfen tolerance. Less than 6% of the applied 14C-acifluorfen was absorbed. There were no differences in acifluorfen absorption between susceptible and tolerant somaclones. More 14C-acifluorfen was translocated in the susceptible than the tolerant somaclones. The susceptible somaclone did not metabolize acifluorfen while some somaclones (i.e.,EBN-3A) metabolized 14C-acifluorfen. Nomenclature: Acifluorfen, 5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoic acid; eastern black night shade, Solanum ptycanthum Dun.,#3 SOLPT.