A few importers of marine products has practiced ice glazing of frozen fish and forced water injection into small octopus to increase their weight. These rampant practices have recently become a serious social issue. Therefore, this study was conducted to develop non-destructive detection methods for verifying forced water injected frozen small octopus using dielectric properties. The weight and dielectric properties of live small octopuses imported from China were measured using an electronic scale and dielectric probe connected with vector network analyzer, respectively. The frequency range from 0.5 to 4 GHz was used for measurement of dielectric properties of small octopus samples. The moisture contents of live small octopuses were determined by convection drying at 105°C for 24 h. To increase weight of small octopus samples, each sample was placed in a container including 2% polyphosphate solution of 800 mL and was kept for 24 h in a refrigerator. Then, the sample was removed from the solution and was frozen at -35°C for 24 h. The moisture contents of live small octopuses were 81.6 ± 1.9% (wb). Regardless of weight of live small octopuses, dielectric constant (ε′) and dielectric loss factor (ε′′) were from 76.09 to 58.62 and from 101.95 to 28.72, respectively. The average weight gain of small octopuses immersed in the solution was approximately 40.6%. Dielectric constant (ε′) and dielectric loss factor (ε′′) of forced water injected small were from 78.18 to 66.71 and from 51.96 to 25.05, respectively. In addition, there was a significant difference between dielectric properties and penetration depth of fresh frozen and forced water injected frozen small octopuses. The results clearly showed that dielectric properties of small octopuses affected by moisture contents could be an important factor to detect forced water injected frozen small octopuses.

Morphology of antennal sensilla and their distribution were investigated in male and female adults of Ooencyrtus nezarae, an egg parasitoid of Riptortus pedestris, using scanning electron microscopy. Antennae of O. nezarae was composed of scape, pedicel and seven flagella in both sexes. Six types of sensilla (s. trichodea, s. basiconica, s. chaetica, s. campaniformia, s. sickle-shaped and unknown s.) were identified from both sexes. Among them, s. trichodea and s. sickle-shaped were multiporous, others are not. They distributed in varying numbers. Sexual dimorphism was clearly observed in the distribution of s. trichodea (only on male antennae) and unknown sensilla (only on female antennae). These findings would be helpful for further studies on detailed sex specific-receptive functions of each antennal sensilla.

Euzophera batangensis (Lepidotera: Pyralidae) is seriously damaging trunks or branches of persimmon tree (Diospyros sp.). We tested the attractiveness of (Z)-9-tetradecen-1-ol (Z9-14OH) and (Z9,E12)-tetradeca- 9,12-dien-1-ol (Z9,E12-14OH) with single or blended baits at southern parts of Korea in 2014 and 2015. The monoene was not attractive at all at three places during the two years. In 2014, diene was equally or more attractive than the mixture in Jinju and Suncheon, respectively. In 2015 too, the attractiveness of diene and mixture was not different in Jinju and Munsan. Monitoring of the seasonal occurrence of E. batangesis with the sex pheromone components revealed that it occurred three times a year; the first occurrence from early April to mid May, the second from early Jun to mid July, and the third from late August to late September.

Ooencyrtus nezarae lshii (Hymenoptera: Encyrtidae) is a major egg parasitoid of Riptortus pedestris (F.) (Hemiptera: Alydidae). One of the components of the aggregation pheromone of R. pedestris, (E)-2-hexenyl (Z)-3-hexenoate (E2HZ3H), is known to attract O. nezarae female. Response of O. nezarae females to E2HZ3H and its isomers was studied using electro-antennogram (EAG) and field tests. O. nezarae females showed higher antennal response to E2HZ3H and Z2HE3H isomers than E2HE3H and Z2HZ3H. In field tests, O. nezarae females were attracted to E2HZ3H traps, but not to Z2E3 traps. Interestingly, the blend of E2HZ3H and Z2HE3H showed significant antagonistic activities to the attraction of O. nezarae females in the field. We conclude from this study that Z2HE3H is a potent antagonist to the behavioral response of O. nezarae.

Chitin synthase (CHS) is an important enzymatic component, which is required for chitin formation in the cuticles and cuticular linings of other tissues in insects. CHSs have been divided into two classes, class A and B, based on their amino acid sequence similarities and functions. Class A CHS (CHS-A) is specifically expressed in the epidermis and related ectodermal cells such as tracheal cells, while expression of class B CHS (CHS-B) is expressed in gut epithelial cells that produce peritrophic matrices. In this study, we cloned the CHS-A gene from the beet armyworm, Spodoptera exigua. The SeCHS-A mRNA was expressed in all developmental stages and specifically in the epidermis and tracheae tissue by RT-PCR analysis. Expression of SeCHS-A gene was suppressed by feeding double-stranded RNA (dsRNASeCHS-A, 150 ng/larva) in the fifth instar of S. exigua. The suppression of SeCHS-A gene expression significantly induced mortality on pupal stage. Also, larvae fed with dsRNASeCHS-A significantly enhanced pathogenicity of an entomopathogenic fungus, Beauveria bassiana ANU1. These results suggest that the SeCHS-A gene plays an important role in development of S. exigua and dsRNA, which is a specific to SeCHS-A gene, may be applied to effective pest control with B. bassiana.

Oxalic acid has a nematicidal activity against the root-knot nematode Meloidogyne incognita. High producer of oxalic acid was isolated, and then named as Aspergillus niger F22. Oxalic acid production was investigated under various temperatures from 20 – 33oC and rotational speeds in 5 L jar fermenters. Yield of oxalic acid increased with decreasing temperature. The highest yield was obtained at 23oC, showing the yield of oxalic acid of 8.7 g/L, whereas oxalic acid production was least at 33oC. At 20oC, the yield was lower than that of 23oC. At a rotational speed of 300 rpm, serious oxygen depletion was present from 48 - 72 h, resulting in low productivity of 26.2 mg /L·h. When a rotational speed was set at 600 rpm, dissolved oxygen tension was over 40% and oxalic acid production increased up to approximately 55%. Viscosity during the culture differed with temperatures. Viscosity increased with the increment of temperatures. When A. niger F22 was cultured at 23oC, viscosity was 810 cP, which was favorable for oxalic acid production.

Four sympatric tortricid (Lepidoptera) species in New Zealand, Epiphyas postvittana (Walker), Planotortrix octo (Dugdale), Cydia pomonella (L.), and Cydia succedana (Denis and Schiffermüller), were studied for the morphology of antennal sensilla and the chemical communication systems using scanning electron microscopy (SEM), electro-antennogram (EAG), gas chromatography-electroantenno-detection (GC-EAD), single sensillum recording (SSR) and field tests. Antennal flagella of adult have four main types of olfactory sensilla (s.); s. trichodea, s. basiconica, s. auricillica, and s. coeloconica in the four species. The s. trichodea subtype I was longest and male-specific in all the species. The antennae of males of the four species showed characteristic higher EAG, GC-EAD and SSR responses to 12, 14-carbon acetates or alcohol. Some of the selected chemicals selected from the electrophysiological experiments caused significant antagonistic or agonistic activities to the sex pheromone of each species in the field. In this study, the four species showed distinct chemical communication systems according to sub-family, which possibly serve as a factor in species isolation.

This research was conducted to investigate the effect of continuous antagonistic muscle strengthening exercise and Evjenth-Hamberg stretching on the pulmonary function of university students with abnormal transformation of forward head posture(FHP). The results of study subject to the continuous antagonistic muscle strengthening(CAS) group(n=10) and Evjenth- Hamberg stretching(EHS) group(n=10) that was conducted 3 times a week for 6 weeks are as follow. FVC, IVC, and MVV all were shown to be significant in the pre·post comparison between the CAS group and EHS group(p<.05), and significant difference was shown for MW between the two groups(p<.05) in which the CAS group showed better effect. Based on the results above, it is considered that combining continuous antagonistic muscle strengthening exercise has better effect on pulmonary function compared to application of only Evjenth-Hamberg stretching.

It is conceivable that the construction of a convention is in question in a case brought before ICJ and a State that is a party to the convention but not to the case has legal interests which may be affected by the construction given by the judgment in the case. As hinted at in the Whaling in the Antarctic case and the Sovereignty over Pulau Ligitan and Pulau Sipadan case, such a third State might intervene in the proceedings under Article 62 as well as Article 63 of the Statute unless it should be interpreted otherwise. In light of relevant provisions of the Statute and jurisprudence of the Court, this paper explores the question whether such a State has the choice, to submit an application to intervene under Article 62 or to make a declaration of intervention under Article 63.

Lentinula edodes is known by oak mushroom. It has been favored as delicious and nutritious food and the low-calorie food with a high nutritional value. It is also functional food since it contains a material well-known for its medicinal benefits. Since the growth and quality of oak mushrooms are sensitively affected by environmental conditions, an adequate environmental control is very essential to improve the yield and quality under protected cultivation. The main objectives of the study were to investigate cultural characteristics of mycelial growth and in vitro fruiting of Lentinula edodes. The optimum culture media for mycelial growth of L. edodes were PDA and MYA. Similarly, optimum temperature was 25℃. Malt extract(2%) and yeast extract(0.2%) were optimum carbon and nitrogen sources. Optimal culture period was 110~120 days in sawdust medium. Among different five log types, highest mycelial growth and fruiting productivity were observed in Quercus variabilis sawdust(20.9%).

This study aims to reveal the impact of continuous antagonist strengthening( CAS) and Evjenth-Hamberg stretching(EHS) on the cervical mobility in the university students with abnormal transformation of forward head posture( FHP). Our experiment was conducted 3 times a week for 6 weeks in a total of 20 individuals : continuous antagonist strengthening(CAS) group(n=10) & Evjenth-Hamberg stretching(EHS) group(n=10). In a pre and post comparison, both CAS group and EHS group appeared significantly in mSBI and SBA(p<.05) and the two-group comparison showed a significant difference(p<.05) : CAS group showed better effects. Thus, it is considered that the combined use with continuous antagonist strengthening(CAS) had better effects for cervical mobility than Evjenth-Hamberg stretching(EHS) alone.

As demand for Tenebrio molitor increases in animal feed market, improvement of mass-rearing indoor system became more crucial. This study is aimed at establishing the technology for conserving genetic resource of Tenebrio molitor, and increasing the technology utilization. This year, in advance, this experiment was designed for examining the developmental characteristics of eggs and larvae. To research the developmental characteristics under different temperatures, eggs, larvae and papae were treated at 17.5, 20, 22.5, 25, 27.5 and 30°C. At 25°C, larvae were exposed to various photoperiod conditions, LD 14:10, 12:12, and 10:14. The results show that the temperature range which affected hatching was 15~32.5°C. To achieve 70 percent or higher hatching rate 17.5~27.5°C was required. Furthermore, hatching occurred within 10 days, when the eggs were treated in 22.5~27.5°C. In regard of larval development, shorter developmental period, and higher pupation and eclosion rates were observed in 25~27.5°C. The shortest larval period was recorded at 14L:10D. At 10L:14D, in contrast, pupae showed the lowest chance of eclosion. Overall, larval development was optimal in long-day condition.

Ski protein is implicated in proliferation/differentiation in a variety of cells. We had previously reported that Ski protein is present in granulosa cells of atretic follicles, but not in preovulatory follicles, suggesting that Ski has a role in apoptosis of granulosa cells. The alternative fate of granulosa cells other than apoptosis is to differentiate to luteal cells; however, it is unknown whether Ski is expressed and has a role in granulosa cells undergoing luteinization. Thus, the aim of the present study was to locate Ski protein in the rat ovary during luteinizationto predict the possible role of Ski. In order to examine the expression pattern of Ski protein along with the progress of luteinization, follicular growth was induced by administration of equine chorionic gonadtropin to immature female rats, and luteinization was induced by human chorionic gonadtropin treatment to mimic luteinizing hormone (LH) surge. While no Ski-positive granulosa cells were present in preovulatory follicle, Ski protein expression was induced in response to LH surge, and was maintained after the formation of the corpus luteum (CL). Though Ski protein is absent in granulosa cells of preovulatory follicle, its mRNA (c-Ski)was expressed and the level was unchanged even after LH surge. Taken together, these results demonstrated that Ski protein expression is induced in granulosa cells upon luteinization, and suggests that its expression is regulated post-transcriptionally.