Seeds of some barberry species have embryo with physiological dormancy that requires a cold stratification for germination. Berberis amurensis Rupr. is a native species of Japan, Korea, the Russian far east, and parts of China. This specific plant is important for its edible fruits and rhizomes with high medicine value. This study aimed to determine the effect of stratification on germination and physiological change of B. amurensis. Seeds were placed on sterilized sand medium moistened with distilled water in 9 cm diameter petri dishes and stored at 4 and 25˚C for 0, 15, 30, 45 and 60 days. Each treatment had 40 seeds per replica, and three repetitions per treatment. Immediately after stratification, total phenolics contents (TPC) was analyzed and seeds incubated at 15/6˚C for 12 weeks. Warm stratification had a significant effect on seed forcing for germination than cold stratification treatment. At 25˚C for 60 days, stratified seed showed highest germination percentage (25.7±4.3%) and germination started in 14 days of the stored period. Whereas TPC was significantly decreased with increasing stored period. Contrastively, cold stratification had no effect on the germination ability. In the same way germination percentage of non-stratified seeds were also zero. The results confirmed that B. amurensis seeds were in a dormant state and warm stratification increased the germination ability by breaking of dormancy.

표면발현(surface-display system)은 세포 또는 바이러스 표면에 목적 단백질을 고정하여 발현시킴으로써 목적 단백질에 대하여 독립적인 공간 구조 및 생물학적 활성을 부여하는 단백질 공학 기술이다. 또한 이를 이용하여 높은 중화항체 유도 및 대량생산이 가능한 삼량체의 형태로 항원 단백질의 발현 또한 가능하다. BES(baculovirus expression system)에서의 표면발현 기술은 번역 후 수정과정 및 복잡한 구조의 다양한 단백질의 발현이 가능하기 에 다른 숙주 기반 시스템보다 효율적이라고 보고되고 있다. 그러나 목적 단백질 외의 다른 표면 단백질과 발현 공간에서의 경쟁으로 목적 단백질의 낮은 생산량이 큰 문제점으로 지적되고 있다. 따라서, 이러한 BES에서 표면 발현의 생산 효율을 증대시키기 위하여, 동일한 표면 공간에 대한 단백질 간의 발현 경쟁에 대해 실험적으로 확인 후, 그를 해결하기 위하여 표면발현에 최적인 목적 단백질 발현을 위한 프로모터 선발 실험을 수행하였다. 이를 통해 BES에서 표면발현에 의한 목적 단백질의 생산 효율을 증대시킬 수 있음을 확인하였다.

In Korea livestock farms breeding cattle have been suffering from re-emerging problems of Brucella (B.) abortus infection while steady decline of bovine brucellosis. Therefore, this study underscored the identification and association of etiological agent of brucellosis in cattle in South Korea. The incidence of brucellosis in cattle was analyzed by bacteriological and molecular methods in 187 brucellosis-suspicious farms of 11 regions between 2018 and 2020. Brucella isolates from various specimens were identified by Brucella-specific multiplex PCR. Epidemiological data were collected by local official veterinarians through history taking from farmers and animal data systems. In 230 of 560 cattle (40.9%) and 94 of 187 farms (50.3%), a total of 313 B. abortus were isolated from various specimens, the majority of isolates were from supramammary lymph node (41%). In epidemiological findings, the majority of positive cases were mainly caused by resurgence (43.7%) and unknown (37.2%). Of 94 positive cases isolated B. abortus, abortion in cattle infected by B. abortus occurred in 51 farms (54.3%) where led to resurgence in 30 farms and environmental survival of B. abortus in 9 farms. Consequently, these findings revealed the existence of etiological agent of bovine brucellosis in Korea, which still occurred at low levels in distinct regions where are allowed to call for persistent biosecurity. Thus, we highlight that brucellosis in Korea needs to take more effective control strategies with potential evidence. Moreover it is ultimately important to maintain a constant monitoring for eradication of brucellosis.

본 연구는 한국근로자를 대상으로 하여 성장욕구가 직무열의와 직무탈진에 영향을 미치는지 조사하였 다. 이와 더불어 부정적 정서조절과 내적 동기가 이들 변수 간의 관계에 영향을 미치는지에 대해서도 검증하였다. 본 연구를 위해 한국의 제조기업에 근무하는 244명의 근로자에게 자기보고식 설문조사를 실시 하였다. 연구결과, 직무열의와 직무탈진에 대한 성장욕구의 직접 효과는 없는 것으로 나타났다. 그러나 이들 변수들 간의 관계는 부정적 정서조절과 내적 동기의 매개역할에 의해 강화되는 것으로 나타났다. 즉 성장욕구와 직무열의의 관계에 대해 부정적 정서조절과 내적 동기는 매개효과를 가졌으며, 성장욕구와 직무탈진의 관계에 부정적 정서조절이 매개효과를 가졌다. 이 결과를 통해 본 연구는 성장욕구와 직무 열의, 직무탈진의 관계에 대한 부정적 정서조절 및 내적 동기와 같은 심리적 특성의 중요성을 확인하였다.

This study aimed to examine the effect of a mild elevation in serum cholesterol level in a porcine coronary overstretch restenosis model using a balloon angioplasty catheter or drug-eluting coronary stent. Pigs were divided into two groups and were fed a commercial normal diet (CND, n = 4) or a high-fat diet (HFD, n = 4) for 5 weeks. Coronary overstretch injury by balloon angioplasty or stent implantation was induced in the left anterior descending and left circumflex artery after 1 week of feeding. Histopathological analysis was performed at 4 weeks after coronary injury. During the experiment, the total cholesterol level in the HFD group increased by approximately 44.9% (from 65.9 ± 3.21 mg/dL at baseline to 95.5 ± 9.94 mg/dL at 5 weeks). The lumen area in the CND group was reduced in comparison with that in the HFD group after balloon angioplasty. After stent implantation, the injury score showed no significant difference. There were significant differences in the neointimal area (2.7 ± 0.33 mm2 in the CND group vs. 3.3 ± 0.34 mm2 in the HFD group, p<0.05), lumen area (2.6 ± 0.54 mm2 in the CND group vs. 2.0 ± 0.33 mm2 in the HFD group, p<0.05), and percent area stenosis (52.0 ± 7.96% in the CND group vs. 62.4 ± 5.15% in the HFD group, p<0.05). Body weight change was not different between the two groups. Increased serum cholesterol level activated vascular smooth muscle cell proliferation in the porcine coronary overstretch model.

Cryopreservation of boar semen is continually researched in reproductive technologies and genetic resource banking in breed conservation. For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Recently, polymorphisms reported to be significant association with sperm MOT. This study was conducted to evaluate the SNP in the coding region of ESR1 (g.672C>T inexon 1) as a positional controlling for motility and kinematic characteristics of post-thawed boar semen. To results,The g.672C>T was significantly associated with frozen semen motility and kinematic characteristics. g.158 T>C SNP was high significantly associated with MOT, VCL, VSL and VAP Also, the SNP was low significantly associated with ALH.Therefore, we suggest that theSNP in the coding region of ESR1 (g.672C>T in exon 1) may be used as a molecular marker for Duroc boar Post-thawed semen quality.