표면발현(surface-display system)은 세포 또는 바이러스 표면에 목적 단백질을 고정하여 발현시킴으로써 목적 단백질에 대하여 독립적인 공간 구조 및 생물학적 활성을 부여하는 단백질 공학 기술이다. 또한 이를 이용하여 높은 중화항체 유도 및 대량생산이 가능한 삼량체의 형태로 항원 단백질의 발현 또한 가능하다. BES(baculovirus expression system)에서의 표면발현 기술은 번역 후 수정과정 및 복잡한 구조의 다양한 단백질의 발현이 가능하기 에 다른 숙주 기반 시스템보다 효율적이라고 보고되고 있다. 그러나 목적 단백질 외의 다른 표면 단백질과 발현 공간에서의 경쟁으로 목적 단백질의 낮은 생산량이 큰 문제점으로 지적되고 있다. 따라서, 이러한 BES에서 표면 발현의 생산 효율을 증대시키기 위하여, 동일한 표면 공간에 대한 단백질 간의 발현 경쟁에 대해 실험적으로 확인 후, 그를 해결하기 위하여 표면발현에 최적인 목적 단백질 발현을 위한 프로모터 선발 실험을 수행하였다. 이를 통해 BES에서 표면발현에 의한 목적 단백질의 생산 효율을 증대시킬 수 있음을 확인하였다.

Air conditioning facilities in nuclear power plants use pre-filters, HEPA filters, activated carbon filters, and bag filters to remove radionuclides and other harmful substances in the atmosphere. Spent filters generate more than 100 drums per year per a nuclear power plant and are stored in temporary radioactive waste storage. Plasma torch melting technology is a method that can dramatically reduce volume by burning and melting combustible, non-flammable, and mixed wastes using plasma jet heat sources of 1,600°C or higher and arc Joule heat using electric energy, which is clean energy. KHNP CRI & KPS are developing and improving waste treatment technology using MW-class plasma torch melting facilities to stably treat and reduce the volume of radioactive waste. This study aims to develop an operation process to reduce the volume of bag filter waste generated from the air conditioning system of nuclear power plants using plasma torch melting technology, and to stably treat and dispose of it. It is expected to secure stability and reduce treatment costs of regularly generated filter waste treatment, and contribute to the export of radioactive waste treatment technology by upgrading plasma torch melting technology in the future.

When decommissioning a nuclear power plant, the structure must be made to a disposable size. In general, the cutting process is essential when dismantling a nuclear power plant. Mainly, thermal cutting method is used to cutting metal structures. The aerosols generated during thermal cutting have a size distribution of less than 1 μm. The contaminated structures are able to generate radioactive aerosols in the decommissioning. Radioactive aerosols of 1 μm or less are deposited in the respiratory tract by workers’ breathing, causing the possibility of internal exposure. Therefore, workers must be protected from the risk of exposure to radioactive aerosols. Prior knowledge of aerosols generated during metal cutting is important to ensure worker safety. In this study, the physical and chemical properties of the aerosol were evaluated by measuring the number and mass concentrations of aerosols generated when cutting SUS304 and SA508 using the laser cutting method. High-resolution aerosol measuring equipment (HR-ELPI+, DEKATI) was used to measure the concentration of aerosols. The HR-ELPI+ is an impactor-type aerosol measuring equipment that measures the aerosol number concentration distribution in the aerodynamic diameter range of 6 nm to 10 um in real-time. And analyze the mass concentration of the aerosol according to the diameter range through the impactor. ICP-MS was used for elemental mass concentration analysis in the aerosol. Analytical elements were Fe, Cr, Ni and Mn. For the evaluation of physical and chemical properties, the MMAD of each element and CMAD were calculated in the aerosol distribution. Under the same cutting conditions, it was confirmed that the number concentration of aerosols generated from both materials had a uni-modal distribution with a peak around 0.1 um. CMAD was calculated to be 0.072 um for both SUS304 and SA508. The trend of the CMAD calculation results is the same even when the cutting conditions are changed. In the case of MMAD, it was confirmed that SUS304 had an MMAD of around 0.1 μm in size for only Fe, Cr and Mn. And SA508, Fe, Cr, Ni and Mn were all confirmed to have MMAD around 0.1 μm in size. The results of this study show that a lot of aerosols in the range of less than 1 μm, especially around 0.1 μm in size, are generated when metal is cut using laser cutting. Therefore, in order to protect the internal exposure of workers to laser metal cutting when decommissioning NPPs, it is necessary to protect from nano-sized aerosols beyond micron size.

The fuel fabrication facility has been built and is being operated by KAERI since licensing research reactor fuel fabrication in 2004. After almost 20 years of operation, outdated equipment for fabrication or inspection has been replaced by automated, digitalized ones to assure a higher quality of nuclear fuels. However, the generation of a large amount of radioactive waste is another concern for the replacement in terms of its volume and various types of it that should be categorized before disposal. The regulatory body, NSSC (Nuclear Safety and Security Commission) released a notice related to the classification of radioactive wastes, and most accessory equipment can be classified into the clearance levels, called self-disposal waste. In this study, the practice of self-disposal of metal radioactive waste is carried out to reduce its volume and downgrade its radioactivity. For metal radioactive waste, which is expected to occupy the most amount, analysis status and legal limitations were performed as follows: First, the disposal plan was established after an investigation of the use history for equipment. Second, those were classified by types of materials, and their surface radio-contamination was measured for checking self-disposable or not. After collecting data, the plan for the self-disposal was written and submitted to the Korea Institute of Nuclear Safety (KINS) for approval.

At high temperatures, molten salt has heat transfer properties like water. Molten salt has the characteristics of a strong natural circulation tendency, large heat capacity, and low thermal conductivity. Unlike sodium, molten salt does not react explosively exothermically with air. However, molten salt has a strong tendency to corrode materials, and its properties are easily changed by a sensitive reaction to oxygen and moisture. Therefore, it is necessary to study material corrosion properties and chemical control methods for nuclear fuel salts, which are eutectic mixtures. In this study, the optimal operation method of the thermal convection loop is established to perform the experiments on the molten salt. The process describes briefly as follows. The operation step consists of preparation, purification, transportation, and operation. In the preparation, the step checks the entire structure and equipment (TC, blower, vacuum pump, etc.). And melt the salt mixture at a high temperature (670°C) slowly in the purification step. Before injecting the molten salt, the surface temperature of the entire loop must retain temperature (about 500°C) constantly. Completely melted molten salt in the melting pot is flow along the pipe of the thermal convection loop in the transportation step. Lastly, the convection of molten salt goes to keep by the temperature difference. The thermal convection loop can be utilized for various experiments such as corrosion tests, component analyses, chemistry control, etc.

Molten salt used in the multipurpose molten salt experiment must be of high purity. Depending on the purpose of the experiment, only the base component of the molten salt be used, or a component simulating a nuclear fission product be added to the base component and used. In all cases, an increase in the concentration of impurities such as oxygen and moisture may lead to an erroneous interpretation when analyzing the experimental results. Therefore, molten salt should be purified before use. In this study, the purification of molten salt is described for multi-purpose molten salt experiments. The salt mixture is selected as MgCl2-NaCl and is quantified at a mixing ratio of 43mol%:57mol%. The salt mixture is treated in a glove box environment because of must minimize the reaction of adsorbing oxygen and moisture when the salt mixture is exposed to the atmosphere. MgCl2 is more likely to contain water than NaCl, the purification of the NaCl-MgCl2 mixture is established according to the purification process for removing water from MgCl2. A process for purifying the salt mixture briefly consists as follows: drying moisture, melting salts, purification, removing HCl, and stabilization. Through the process be able to obtain high-purity molten salt and more accurate experiment results.

In Korea livestock farms breeding cattle have been suffering from re-emerging problems of Brucella (B.) abortus infection while steady decline of bovine brucellosis. Therefore, this study underscored the identification and association of etiological agent of brucellosis in cattle in South Korea. The incidence of brucellosis in cattle was analyzed by bacteriological and molecular methods in 187 brucellosis-suspicious farms of 11 regions between 2018 and 2020. Brucella isolates from various specimens were identified by Brucella-specific multiplex PCR. Epidemiological data were collected by local official veterinarians through history taking from farmers and animal data systems. In 230 of 560 cattle (40.9%) and 94 of 187 farms (50.3%), a total of 313 B. abortus were isolated from various specimens, the majority of isolates were from supramammary lymph node (41%). In epidemiological findings, the majority of positive cases were mainly caused by resurgence (43.7%) and unknown (37.2%). Of 94 positive cases isolated B. abortus, abortion in cattle infected by B. abortus occurred in 51 farms (54.3%) where led to resurgence in 30 farms and environmental survival of B. abortus in 9 farms. Consequently, these findings revealed the existence of etiological agent of bovine brucellosis in Korea, which still occurred at low levels in distinct regions where are allowed to call for persistent biosecurity. Thus, we highlight that brucellosis in Korea needs to take more effective control strategies with potential evidence. Moreover it is ultimately important to maintain a constant monitoring for eradication of brucellosis.

본 연구는 한국근로자를 대상으로 하여 성장욕구가 직무열의와 직무탈진에 영향을 미치는지 조사하였 다. 이와 더불어 부정적 정서조절과 내적 동기가 이들 변수 간의 관계에 영향을 미치는지에 대해서도 검증하였다. 본 연구를 위해 한국의 제조기업에 근무하는 244명의 근로자에게 자기보고식 설문조사를 실시 하였다. 연구결과, 직무열의와 직무탈진에 대한 성장욕구의 직접 효과는 없는 것으로 나타났다. 그러나 이들 변수들 간의 관계는 부정적 정서조절과 내적 동기의 매개역할에 의해 강화되는 것으로 나타났다. 즉 성장욕구와 직무열의의 관계에 대해 부정적 정서조절과 내적 동기는 매개효과를 가졌으며, 성장욕구와 직무탈진의 관계에 부정적 정서조절이 매개효과를 가졌다. 이 결과를 통해 본 연구는 성장욕구와 직무 열의, 직무탈진의 관계에 대한 부정적 정서조절 및 내적 동기와 같은 심리적 특성의 중요성을 확인하였다.

This study aimed to examine the effect of a mild elevation in serum cholesterol level in a porcine coronary overstretch restenosis model using a balloon angioplasty catheter or drug-eluting coronary stent. Pigs were divided into two groups and were fed a commercial normal diet (CND, n = 4) or a high-fat diet (HFD, n = 4) for 5 weeks. Coronary overstretch injury by balloon angioplasty or stent implantation was induced in the left anterior descending and left circumflex artery after 1 week of feeding. Histopathological analysis was performed at 4 weeks after coronary injury. During the experiment, the total cholesterol level in the HFD group increased by approximately 44.9% (from 65.9 ± 3.21 mg/dL at baseline to 95.5 ± 9.94 mg/dL at 5 weeks). The lumen area in the CND group was reduced in comparison with that in the HFD group after balloon angioplasty. After stent implantation, the injury score showed no significant difference. There were significant differences in the neointimal area (2.7 ± 0.33 mm2 in the CND group vs. 3.3 ± 0.34 mm2 in the HFD group, p<0.05), lumen area (2.6 ± 0.54 mm2 in the CND group vs. 2.0 ± 0.33 mm2 in the HFD group, p<0.05), and percent area stenosis (52.0 ± 7.96% in the CND group vs. 62.4 ± 5.15% in the HFD group, p<0.05). Body weight change was not different between the two groups. Increased serum cholesterol level activated vascular smooth muscle cell proliferation in the porcine coronary overstretch model.

Cryopreservation of boar semen is continually researched in reproductive technologies and genetic resource banking in breed conservation. For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Recently, polymorphisms reported to be significant association with sperm MOT. This study was conducted to evaluate the SNP in the coding region of ESR1 (g.672C>T inexon 1) as a positional controlling for motility and kinematic characteristics of post-thawed boar semen. To results,The g.672C>T was significantly associated with frozen semen motility and kinematic characteristics. g.158 T>C SNP was high significantly associated with MOT, VCL, VSL and VAP Also, the SNP was low significantly associated with ALH.Therefore, we suggest that theSNP in the coding region of ESR1 (g.672C>T in exon 1) may be used as a molecular marker for Duroc boar Post-thawed semen quality.