Lettuce was a leaf vegetable with short growing term and cold-tolerant plant. We grew it the whole year. The price of lettuce is the most expensive from July to September. But damages by lots of insect pests appear to greenhouse grown lettuce in summer season. In this study, we observed biological controls of western flower thrips(Franklinella occidentalisi) by the predator mites, Amblyseius swirskii and Neoseiulus cucumeris in lettuce greenhouse from July 7 to September 10. Hypoaspis aculerfer larvae and adults were released 30 individuals per m2 at the first one time as lettuce was planted on the greenhouse. We checked the density of F. occidentalis every 10 days on the 30 lettuce leaves. When the population of F. occidentalis reached 1~5 individuals per a leaf, A. swirskii larvae and adults were released 35 individuals per m2 at 1~3 times and Neoseiulus cucumeris larvae and adults also were released 150 individuals per m2 at 2~3 times in each lettuce greenhouse. During the high temperature season, Amblyseius swirskii was more effective biological controls of F. occidentalis than Neoseiulus cucumeris.

Cambodia covers an area of 181,035 sq km, among them, forests cover 53 percent, so there are abundant Insect diversities. Collecting expeditions of Insect were conducted several times from 2009 to 2010 (09. 7.2-7.11, 10.16-22, 12.25-12.30, 10. 4. 30-5.6) in Cambodia sponsored by Natioanl Biological Resources, Korea. Central Cadamon Protected Forest (N 11 41' 13" E 103 26' 31.3"), Seima Biodiversity Conservation Area (N 12° 08' 20.8", E 106 55' 2.7") and Prov. Pursat, Roveang (N 12° 19' 06.5", E 103° 3' 03.8") were main area surveyed by pitfall trap, light trap and sweeping, searching etc. Numerous individuals were collected and more than 150 species belonging to 40 families were founded, among them, there were lots of new and new recorded species. Among Cambodian insects, species of Lepidoptera, Coleoptra and Hymenoptera were preferentially introduced in here. The photos for species of dominant, rare, new and new recorded were provided, and also collecting sites and activities were presented.

Purpose – The purpose of this study is to find out research directions for distribution and fusion and complex field to many domestic and foreign researchers carrying out related academic research by confirming research trends in the Journal of Distribution Science (JDS). Research Design, Data, and Methodology – To do this, I used keywords from a total of 904 papers published in the JDS excluding 19 papers that were not presented with keywords among 923. The analysis utilized word clouding, topic modeling, and weighted frequency analysis using the R program. Results – As a result of word clouding analysis, customer satisfaction was the most utilized keyword. Topic modeling results were divided into ten topics such as distribution channels, communication, supply chain, brand, business, customer, comparative study, performance, KODISA journal, and trade. It is confirmed that only the service quality part is increased in the weighted frequency analysis result of applying to the year group. Conclusion – The results of this study confirm that the JDS has developed into various convergence and integration researches from the past studies limited to the field of distribution. However, JDS's identity is based on distribution. Therefore, it is also necessary to establish identity continuously through special editions of fields related to distribution.

Background : Korean ginseng is an important cash crop in Asian countries. However, plant yield is reduced by pathogens. Among the Ilyonectria radicicola-species complex, I. mors-panacis is responsible for root-rot and replant failure of ginseng in Asia. It is essential to find a way to reveal the existence of the pathogen before starting cultivation. Therefore, qRT-PCR method is developed to detect and quantify the pathogen in ginseng soils. Methods and Results : In this study, species specific Histone H3 primer set is developed for the quantification of I. mors-panacis. The primer set was applied on DNA of other microbes to evaluate its sensitivity and selectivity on I. mors-panacis DNA. Sterilized soil samples artificially infected by the pathogen in different concentrations were used to evaluate the ability of the primer set to detect the pathogen population in the soil DNA. Finally, the pathogen was quantified in many natural soil samples. The designed primer set was found to be sensitive and selective to I. mors-panacis DNA. In artificially infected sterilized soil samples, the estimated template using qRT-PCR was positively correlated with the pathogen concentration in soil samples (R2=0.94), disease severity index (R2=0.99), and colony forming unit (R2=0.87). In the natural soils, the pathogen was recorded in the most of fields produce bad yields with the range of 5.82 ± 2.35 to 892.34 ± 103.70 pg/g of soil. Conclusion : According to the presented results, the proposed primer set is applicable for estimating soils quality before ginseng cultivation. This will help in the disease management and crop protection in the future.

Background : Panax ginseng C.A.Meyer is a perennial herb and its growth potential keep relatively high at least for the early 3 years old. If the transplanted ginseng roots of 2 or 3 years old grow well, a period of cultivation and the disease management cost could be reduced for the production of 6 years old ginseng roots. Methods and Results : The rooting and growth of a transplanted ginseng root could be affected by the age of a transplant. Therefore, the experiments were conducted to examine the influences of the age factor on the rooting and growth of transplanted ginseng roots. The ginseng roots with different plant ages in growth years (1, 2, and 3 years old), used in this study, were obtained by raising in a seedling production field near the Punggi Ginseng Experiment Station. The transplants were continuously grown under the same conditions after transplanting. The number of lateral or branched roots at the root-growing period after transplanting was basically higher in originally older plant ages compared to younger plants: the numbers of secondary and tertiary branched roots of the 1 year-old transplants were 8, 12, respectably; 2 years old: 12, 20; 3 years old: 12, 28. Those did not show any difference in both overground and underground growing, in compared to conventional cultivations with roots of a year old. The plants grew well without anthracnose, spotting disease, and high temperature injury. Conclusion : The conventional cultivation period of above 5 or 6 years for the raw material of red ginseng might be shorten and also the cost of disease control could be decreased, by transplanting of 2 or 3 years old roots raised at a seedling nursing field.

A variety of genetically modified (GM) crops have been developed in Korea. In these crops, the resveratrol-enriched transgenic rice plant has moved ahead to generate the dossier for regulatory review process required for commercialization of GM crop. The resveratrol-enriched transgenic rice plant could be released to farmers for cultivation after national regulators have determined that it is safe for the environment and human health. Here we developed a PCR-based DNA marker based on flanking sequences of transgene for the discrimination of zygosity in resveratrol-enriched transgenic rice plant. This DNA marker will be useful for identifying of resveratrol-enriched transgenic rice plant, and can also be use to estimate transgene movement occurred by pollen transfer or seed distribution.

Clubroot is a devastating disease caused by Plasmodiophora brassicae and results in severe losses of yield and quality in Brassica crops including Brassica oleracea. Therefore, it is important to identify resistance gene for CR disease and apply it to breeding of Brassica crops. In this study, we applied genotyping-by-sequencing (GBS) technique to construct high resolution genetic map and mapping of clubroot resistance (CR) genes. A total of 18,187 GBS markers were identified between two parent lines resistant and susceptible to the disease, of which 4,103 markers were genotyped in all 78 F2 plants generated from crossing of both parent lines. The markers were clustered into nine linkage groups spanning 879.9 cM, generating high resolution genetic map enough to refine reported reference genome of cabbage. In addition, through QTL analysis using 78 F2:3 progenies and mapping based on the genetic map, two and single major QTLs were identified for resistance of race 2 and race 9 of P. brassicae, respectively. These QTLs did not show collinearity with CR loci found in Chinese cabbage (Brassica rapa) but roughly overlapped with CR loci identified in cabbage for resistance to race 4. Taken together, genetic map and QTLs obtained in this study will provide valuable information to improve reference genome and clubroot resistance in cabbage.

The main purpose of the research is to suggest a new business model of direct distribution of organic agricultural products cultivated in suburban area. To secure competitiveness, logistics cost reduction is important through direct transaction of organic agricultural products cultivated on suburban area. The research addresses food safety, farmers' income and unemployment issues with Eco-M business model. The research methods include investigation on the followings: current domestic agriculture; related problems on the existing literature reviews; the current status of suburban agriculture; specialty store of eco-friendly agricultural products. Eco-M business model can solve various problems of suburban agriculture supply system, challenges of organic farming specialty stores, unemployment issues, and difficulties of processing verticalization. Future research should include followings. First, there should be comparative analysis between businesses of environmentfriendly specialty stores through suburban agriculture and producer's agriculture connected with organic farming market. Second, it is required to develop strategy of environment-friendly specialty stores. Finally, analysis of the potential of Sextic industries is necessary throughout production-process-service process.

Purpose – This study classified consumers' value inclination to find out ways to enhance consumers' eco-friendly product purchase intention. Further, it verified the differences among eco-friendly product purchase intentions depending upon value inclination. Research design, data, and methodology – The structured model and hypotheses were established, and 202 copies of effective questionnaires were used. In order to verify the hypotheses, we used single regression analysis, multiple regression, 3-step mediating regression, and path analysis. Results – Individualism had a positive influence upon materialism, need for uniqueness, and face wants, and collectivism had a positive influence upon materialism only. Factors of self-expressive consumption inclination had a positive influence upon eco-friendly product purchase intention, and factors of value inclination also had a positive influence. Finally, self-expressive consumption inclination mediated between value inclination and eco-friendly product purchase intention. Conclusion – Consumers with individualism inclination felt the need to connect the ownership of an eco-friendly product with their extended self and, further, it was clear that not only the government but also enterprises should build up their public image regarding eco-friendly products.

Mungbean (Vigna radiata) is a fast-growing, warm-season legume crop that is primarily cultivated in developing countries of Asia. We constructed a draft genome sequence of mungbean to facilitate genome research into the subgenus Ceratotropis and to enable a better understanding of the evolution of leguminous species. The draft genome sequence covers 80% of the estimated genome, of which 50.1% consists of repetitive sequences. In total, 22,427 high confidence protein-coding genes were predicted. Based on the de novo assembly of additional wild mungbean species, the divergence of what was eventually domesticated and the sampled wild mungbean species appears to have predated domestication. Moreover, the de novo assembly of a tetraploid Vigna species (Vigna reflexo-pilosa var. glabra) provided genomic evidence of a recent allopolyploid event. To further study speciation, we compared de novo RNA-seq assemblies of 22 accessions of 18 Vigna species and protein sets of Glycine max and Cajanus cajan. The species tree was constructed by a Bayesian Markov chain Monte Carlo method using highly confident orthologs shared by all 24 accessions. The present assembly of V. radiata var. radiata will facilitate genome research and accelerate molecular breeding of the subgenus Ceratotropis.

Resveratrol rice Iksan526 was developed by overexpession of T-DNA (RB::P-Ubi::RS::T-NOS::P-35S::PAT::T-35S::LB) in rice variety Dongjin. To confirm one locus insertion of T-DNAs, Mendelian genetic analysis was carried out on selection marker bar gene and objective RS gene separately by using a F2 population derived from a cross of Dongjin/Iksan526 (T6). A total of 450 four-leaf-old plants from F2 population were treated by 0.3% basta, and a phenotypic separation ratio of 3:1 (321 survival: 129 dead, p>0.90) complied with Mendelian inheritance indicating one locus insertion of bar gene. Genotypic separation was analyzed by using PCR with specific primers for 300 plants, which were selected from 321 survival plants after phenotypic separation. Results revealed a ratio 1:2 of homologous to heterozygous (92:208, p>0.90), which further confirmed one locus insertion of RS gene. In addition, comparison on agronomic traits and resveratrol contents between transgenic rice and the donor variety were launched to evaluate the phenotypic performance over multi-generations (years).

Nesfatin-1/NUCB2, which is associated with the control of appetite and energy metabolism, was reported for the first time to be expressed in the hypothalamus. However, recent studies have shown that nesfatin-1/NUCB2 was expressed not only in the hypothalamus, but also in various tissues including digestive and reproductive organs. We also demonstrated that nesfatin-1/NUCB2 was expressed in the reproductive organs, pituitary gland, heart, lung, and gastrointestinal tract of the adult mouse. However, little is known about nesfatin-1/NUCB2 expression in fetal and neonatal mice. Therefore, we examined here the distribution of nesfatin-1/NUCB2 in various organs of fetal and neonatal mice and compared them with the distribution in adult mice. As a result of immunohistochemical staining, nesfatin-1/NUCB2 protein was expressed relatively higher in the lung, kidney, heart, and liver compared to other organs in the fetus. Western blot results also showed that nesfatin-1/NUCB2 protein was detected in the lung, kidney, heart, and stomach. Next, we compared the expression levels of nesfatin-1/NUCB2 mRNA in the fetus and neonate with the expression levels in both male and female adult mice. The expression levels in heart, lung, stomach, and kidney were higher compared with other organs in fetal and neonatal mice and in both male and female adult mice. Interestingly, the expression of nesfatin-1/NUCB2 mRNA in the kidney was dramatically increased in male and female adult mice compared to fetal and neonatal mice. These results indicate that nesfatin-1/NUCB2 may regulate the development and physiological function of mouse organs. In the future, we need more study on the function of nesfatin-1/NUCB2, which is highly expressed in the heart, lung, and kidney during mouse development.

In this study, oocyte steroidogenesis are investigated in relation to oocyte development in the starry flounder, Platichthys stellatus, a marine multiple spawner. Vitellogenic (0.52 and 0.55 mm oocyte diameter) and mature oocytes (0.63, 0.66 and 0.71 mm oocyte diameter) were incubated in vitro in the presence of [3H]17α-hydroxyprogesterone ([3H]17α- OHP) as a precursor. Steroid metabolites were extracted from the incubated media and oocytes, the extracts were separated and identified by thin-layer chromatography (TLC), high performance liquid chromatography (HPLC) and gas chromatographymass spectrometry (GC-MS). The major metabolites produced from [3H]17α-OHP were androgens [androstenedione (A4) and testosterone (T)] and estrogens [17β-estradiol (E2) and estrone (E1)] and progestins [17α,20α-dihydroxy-4-pregnen- 3-one (17α20αP) and 17α,20β-dihydroxy-4-pregnen-3-one (17α20βP)] in vitellogenic and mature oocytes. The results from this study suggest the potential roles of E1 in the oocytes with diameter 0.52-0.71 mm, 17α20αP and 17α20βP at the oocytes of 0.63, 0.66 and 0.71 mm.