Insulin in vertebrates plays a crucial role in maintaining homeostasis of blood sugar level. Insulin-like peptide (ILP) has been identified in insects, such as Drosophila melanogaster and Aedes aegypti. Plasma sugars and polyols of the diamondback moth, Plutella xylostella were separated by a Bio-LC. Among seven peaks, trehalose was the most predominant blood sugar and maintained at approximately 3.5 mM in the larval plasma. However, the feeding activity affected the plasma trehalose level, in which starvation significantly up-regulated the trehalose level. Analysis of ILP expression upon feeding indicated that feeding stimulated the gene expression of ILP. Interestingly, an injection of a vertebrate insulin significantly suppressed the hypertrehalosemia induced by starvation. These results suggest that ILP is a endocrine signal to down-regulate the plasma trehalose level in P. xylostella.

The cotton aphid, Aphis gossypii (Glover), is one of the main pests in various vegetable crops due to insecticide resistance in Korea. Some insect pests noticed neonicotinoid insecticide resistance such as Nilaparvata lugens, Bemisia tabaci and Myzus persicae etc. and the major player which contributed for developing the resistance was over-expression of P450, particularly CPY6 family. However, A. gossypii was a unique case that they developed non-P450 dependent resistant mechanism. Previously we reported that two point mutations (RtoT in nicotinic acetylcholine receptor, nAChR, beta 1 subunit and RtoT with LtoS in a transcript variant) contribute to develop the imidacolprid resistance in A. gossypii. Moreover we surveyed the mutation(s) in various local field populations. Based on the 3D modeling, we hypothesize that RtoT mutation can reduce the imidacloprid sensitivity. A stretch of 33 amino acid was deleted in the N-terminal region of original transcript of nAChR beta 1 subunit that contained RtoT with LtoS mutations in resistant strain. Among the two transcripts, only original transcript differently expressed between imidacloprid susceptible and resistant strain (resistant ratio = 3,800). Six alpha subunit (1∼5, 7) transcript levels were not significantly different between two strains. Therefore mutation and down-regulation of nAChR beta 1 subunit is also associated with imidacloprid resistance in the A. gossypii.

Emotion regulation has been suggested as a pathway through which personality traits (e.g., extraversion or neuroticism) affect psychological well-being. However, the pathway needs further investigation across cultures due to variations in parts of the relationship reported in recent culture research. With an aim of improving current understanding of the pathway, we investigated the role of emotion regulation mediating the link between personality traits and well-being across two college samples from different cultural backgrounds: Korea and the United States (US). Results of mediation analysis revealed that the extraversion—well-being relationship was fully mediated by the degree to which individuals regulate negative emotions in both Korean and US samples. However, the neuroticism— well-being relationship was partially mediated by emotion regulation in the US sample, while it was fully mediated in the Korean sample. The role of emotion regulation differently functioning across cultures suggests the importance of investigating cultural-specific mechanism of psychological processes.

소말리아 해적에 의한 해운에 대한 위협의 증가는 해운선사의 민간해상보안회사(PMSC) 사용을 크게 증가시키는 계기가 되었다. 민간해상보안회사의 사용은 소말리아 해적의 위협에 대해 상선을 보호하기 위한 매우 효과적인 해적대책이라고 판단된다. 그러나 해상에서 민간해상보안회사와 민간무장보안요원(PCASP)의 사용을 둘러싼 여러 가지 법적 및 실질적인 문제가 발생하고 있다. 본 연구는 인도양과 아덴만 해역의 소말리아 해적행위로부터 상선을 보호하기 위한 민간해상보안회사와 민간무장보안요원의 승선에 관한 국제협약, 지침 및 권고상의 민간무장보안요원의 규제에 관한 규정 및 그 문제점을 분석하고 이에 대한 해결방안을 제시하는데 있다. 특히, 민간무장보안요원의 승선에 관한 관할권 문제, 정당방위에 있어서 민간무장요원에 의한 무력사용의 권한 및 민간해상보안회사의 사용에 대한 승인 권한을 중심으로 검토 및 분석한다. 본 연구의 결과 현재 소말리아 해적행위로부터 선박을 보호하기 위한 민간해상보안회사와 민간무장보안요원의 사용에 관한 법률체계가 복잡하고, 때로는 애매하거나 불일치 및 유동적인 상태인 것으로 나타나고 있다. 따라서 유엔해양법협약상의 민간무장보안요원의 승선에 관한 현행 규칙의 해석과 새로운 규칙의 제정에 관한 법률체계를 조정하기 위한 국제적인 노력이 필요하다고 판단된다. 또한 민간해상보안회사를 규제하기 위한 관련 국내법을 시급히 제정할 것을 제안하였다.

The present study assessed the effect of FSH and LH on oocyte meiotic, cytoplasmic maturation and on the expression level and polyadenylation status of several maternal genes. Cumulus-oocyte complexes were cultured in the presence of FSH, LH, or the combination of FSH and LH. Significant cumulus expansion and nuclear maturation was observed upon exposure to FSH alone and to the combination of FSH and LH. The combination of FSH and LH during entire IVM increased the mRNA level of four maternal genes, C-mos, Cyclin B1, Gdf9 and Bmp15, at 28 h. Supplemented with FSH or LH significantly enhanced the polyadenylation of Gdf9 and Bmp15; and altered the expression level of Gdf9 and Bmp15. Following parthenogenesis, the exposure of oocytes to combination of FSH and LH during IVM significantly increased cleavage rate, blastocyst formation rate and total cell number, and decreased apoptosis. In addition, FSH and LH down-regulated the autophagy gene Atg6 and upregulated the apoptosis gene Bcl-xL at the mRNA level in blastocysts. These data suggest that the FSH and LH enhance meiotic and cytoplasmic maturation, possibly through the regulation of maternal gene expression and polyadenylation. Overall, we show here that FSH and LH inhibit apoptosis and autophagy and improve parthenogenetic embryo competence and development.

UN 해양법협약을 계기로 하여 국제사회에서는 새로운 해양질서가 확립되었고 이에 따라 개방으로 인한 국가간 교류가 지속적으로 증가하였다. 이로써 어느 정도 해양범죄에 대한 대응여건이 국제사회에 조성되었다고 평가되기도 한다. 어떤 이유에서건 국제사회는 해양이용인구가 확대되고 있고 해양은 그 특성상 범죄로 악용될 경우에 규모가 대규모일 것임은 쉽게 짐작할 수 있다.해양과 관련하여 불법어업은 이미 예전부터 문제가 되어왔고, 다양한 협약을 통해 제재조치가 이루어지고 있었다. 하지만 그럼에도 불구하고 여전히 그 범죄의 비율은 줄어들지 않고 있다. 게다가 단순한 해양생물의 대량어획을 통한 이익추구에 그치는 것이 아니라 조직범죄단체와 연계되어 그들의 마약밀매, 밀입국, 돈세탁 등과 같은 대규모 범죄행위와 연계되어 매개체 내지는 자금확보의 수단이 되고 있다. 즉 기존에 염려하던 환경적 문제에서 더 나아가 다양한 문제로 확대되고 있는 것이다. 이러한 움직임으로 인해 이미 유엔 총회에서는 인터폴을 통한 IUU 어업행위의 규제를 제안한 바 있다. 그러나 국제사회에서 문제삼고 있는 기존의 범죄행위와는 달리봐야하며 RFMO와 FAO의 제재조치에 맡겨둠이 합리적이라는 반대에 부딪혀 잠시 일단락된 상태이다. 하지만 이러한 UN과 미국의 주장은 국제범죄로써 보호할 가치있는 것임은 IUU 어업의 현황을 살펴보면 알 수 있다. 단순히 해양생태계 문제에 그치지 않고 다양한 분야의 범죄행위의 전제 내지는 원천이 되고 있는 IUU 어업의 국제형사범죄화의 문제는 다시금 생각해 볼 만한다.

인터넷이 상품거래의 새로운 공간으로 등장한 것은 이미 오래 전의 일이고, 최근 일부 분야는 오 히려 오프라인 시장 보다 온라인 시장이 상품거래 의 주된 무대가 되고 있다. 종래부터 제조업자는 자 신이 제조한 상품이 유통업자에 의해 다시 판매될 경우에 적용되는 이른바 재판매가격에 대하여 일 정한 제한을 가하려는 시도를 해왔고, 이러한 재판 매가격유지행위(RPM)에 대하여는 공정거래법에 의해 일정한 규제가 이루어져 왔다. 이러한 현상은 인터넷 상거래의 경우에도 동일하게 발생할 수 있 고 실제로 미국에서는 이른바 인터넷최소광고가격 통제행위(IMAP)를 어떻게 취급할 것인지에 대한 논의가 한창 이루어지고 있다. 우리 공정거래법은 수직적 경쟁제한행위 중 가 격제한행위와 비가격제한행위를 각각 다르게 규율 하고 있고, 위 인터넷최소광고가격통제행위는 표 면적으로는 광고에 대한 제한으로서 비가격적 제 한행위의 성격을 가지지만, 실질적으로는 가격에 영향을 미치는 특성이 존재하는바, 이 논문에서는 우리 법상 이를 어떻게 취급할 것인지에 대하여 다 루어 보고자 한다. 논의에 앞서 재판매가격유지행 위와 관련한 기존의 당연위법의 원칙과 최근 미국 연방대법원 및 우리 대법원판결에 의해 제시된 합 리의 원칙에 관하여 살피기로 한다.

Early onset torsion dystonia is caused by mutations in DYT1 gene in humans. The molecular and cellular etiology underlying this disorder is not still understood yet. Because vertebrates have more than 4 homologs in their genomes, it is very hard to elucidate the exact in vivo functions of Torsin1A. Instead, Drosophila has only one homolog named Torsin. To investigate the in vivo functions of Torsin, we generated and characterized transgenic flies expressing coding regions of Drosophial Torsin (DTor) cDNA or double stranded inhibitory DNA constructs (RNAi). The transgenic expression of DTor cDNA or RNAi in all tissue induced significant changes in DTor proteins levels as well as ability of motor controls. In addition, DTor over-expressing flies showed increased resistance to H2O2 or paraquat. In the future study, we will found how those phenotypes were accomplished by performing various experiments.

Many insects adjust feeding behavior to meet their optimal requirement for multiple nutrients. In the present study, we investigated the behavioral regulatory responses of male and female adults of an omnivorous beetle, Tenebrio molitor (Coleoptera: Tenebrionidae), using choice and no-choice feeding experimental designs. In the choice experiment, newly eclosed adults of both sexes received one of four food-pairing treatments and so were allowed to self-compose their preferred nutrient intake by selecting between two nutritionally-complementary diets (protein-rich vs. carbohydrate-rich diet; 35:7 vs. 7:35, 35:7 vs. 5.6:28, 28:5.6 vs. 7:35, or 28:5.6 vs. 5.6:28, P%:C% by dry mass). Our data showed that both sexes independently regulated their intake of protein and carbohydrate to a 1:1 ratio, indicating that they are capable of adjusting their feeding behavior to optimize their nutrient preference. In the no-choice experiment, adults were forced to eat one of seven single diets that varied in P:C ratio (0:42, 7:35, 14:28, 21:21, 28:14, 35:7, or 42:0). Results showed that both sexes ingested similar amounts of nutrients when the dietary P:C ratio was moderately balanced (14:28, 21:21 and 28:14), but males ingested significantly more than females on those diets that were extremely imbalanced with respect to their P:C ratio (0:42, 7:35, 35:7 and 42:0). This indicates that there exists a sex-specific difference in nutrient intake when the nutritional balance of diets deviates away from the optimal level under restricted feeding condition.

The article 18 of game review regulation has some problems related to mandate of upper law; it violates the limit of mandate of upper law and judicial power. The article 2 of Game industry promotion law defines the concept of ‘gambling behavior’. But the article 18 of game review regulation includes behaviors which are not included in the article 2 to regulate them. Court should have the power to interpret which is ‘gambling behavior’ or not; according to the article 18 of game review regulation, the game rating board has the power to interpret which is ‘gambling behavior’ or not. According to the revision of Game industry promotion law, rating rejection to the violation of Game industry promotion law is possible. Minor violation of law as to business regulation should not be considered as the reason of rating rejection. Therefore, The revision of game law has probability of violating Constitution principle such as proportion rule.

This study challenges constitutional problems of the identification regulation on online web-board Games, which is about to be reviewed by Ministry of Government Legislation. The regulation comprises imposition of duty for online web-board game publisher to identify every game user whenever the game user plays or accesses the online web-board game. The regulation is deemed to be unconstitutional because 1) it is not an effective mean to prevent online web-board game from being misused as gambling 2) it violates free speech right, right on personal information as well as privacy. As the Constitutional Court of Korea has already decided that internet real-name system, which imposed identification duty online bulletin board, is unconstitutional, any regulator or legislator should not use identification regulation without eliminating its unconstitutionality.

Interpretation of the laws governing speculation in Korea has been discussed primarily based on the judiciary judgment over the concept of speculation where the courts and statutes have been consistent in applying the existence of “cash convertibility” as the criterion in determining the speculative nature of an activity. This has applied to the Korean gaming regulations in such a way that a game is to be prohibited if it meets this speculative condition but allowed otherwise. But amendments of the Game Industry Promotion Act’s enforcement ordinance in 2013 to prohibit “auto-play” etc., has brought out a new political problem in the government’s regulation against speculation in online poker games, as the new amendment was devised to prevent excess-use by the users of online poker games that do not meet the definition of speculation such as gambling, under the existing provisions. This study examines and tests the banning of “auto-play” that would not be suitable for Online games. And it shows the ineffectiveness and lots of side effects that game industries confront on many aspects.

The matching system of online games is important factor that determines enjoy the game with others and defines the features of the online game itself. In this regard, Regulation to force the random matching of Web-based board game that has recently been made in Korea, have some problems in terms of both the development and use. First of all, regulation directly to the matching method, shall preclude the development of a creative matching system in social game environment . In addition, the antipathy of fun and discomfort of the game use is a concern in the position of the user. Games for direct regulation affecting the development and use as much considering it prudent approach is required.

In general, a wagering is deemed a form of gambling when it has the element of betting on properties. Also, any game that leads to financial loss or gain based on its outcome is also interpreted as a form of gambling. However, what if there is no loss or gain of profit in property? In Korea, this kind of non gambling game is widely called Web Board Game. In recent years, Korea’s game industry has become the new engine for economic growth and positioned itself as the symbol of a successful cultural content. However, despite all this, the popular media has accused game as the cause of gambling. And every year the government is coming out with new regulations that woule be seriously affecting the whole game industry and game developer's life. The most recent regulations are coming from Amendments of Game Industry Promotion Act's Implementing Ordinance in the year of 2013. This study examines and tests the Problem of "webboard-game's daily limit regulation" which is known as the most difficult for developers to materialize from these 2013 years amendments of regulations. And it shows the effectiveness and provides an alternative direction for game development.

In recent years, the government is trying to regulate the web board games - poker, gostop etc. with the amendment of the executive order regarding the game law. The main regulation of this order is to regulate the maximum amount of betting the game as a ten thousand won per game and a hundred thousand won a day. if these are not obeyed, the gamer and game company will be punished. Therefore, these regulations need to be reviewed in terms of legal issues with a strict examination. However the regulation on the limits of betting in a game seems not to be justified with the constitutional standards - the principle of apparentness (definitude), the principle banning excessive enforcement when restricting the basic rights of people - in this case, the general human rights and liberties to act of gamers and the freedom of profession and speech of game operators(company). It is also questioned that it might infringe the essense of the legal institution of game and rights, and cause the paradox of excessive regulation.

우리나라에서 악취 규제는 배출원의 부지경계선과 배출구에서 농도 규제치를 사용한다. 그러므로 수용체에서 정확한 체감악취의 유무를 쉽게 판단하기는 곤란하다. 비록 개개인의 악취에 대한 응답을 결정하는 변수는 다양하고 응답 종류도 광범위하게 나타나지만, 일반적으로 악취에 대한 규제는 다양한 변수를 고려하여 구성되며, 이들 변수에는 악취 발생빈도, 강도, 기간, 불쾌도, 지역 변수 등이 있다. 본 연구에서는 매사추세츠(미국), 뉴질랜드, 덴마크, 네덜란드, 호주 서부, 타이완 등 6개 지역의 악취 규제를 사용하여 소각장 주변에서 악취 영향 거리를 비교하였다. 악취 영향 거리를 평가하기 위하여 이들 6개 지역 규제는 악취 농도와 허용 빈도를 고려하고 있다. 연구 결과에 의하면 악취에 의한 영향 거리는 0.5~1.4 km의 범위로 나타났다. 악취 농도를 고정하고 허용 빈도를 변경하는 경우 허용 빈도가 높을수록 영향 거리는 크게 나타났다. 허용 빈도를 고정하고 농도를 변화시키는 경우 농도가 높으면 영향거리가 줄어들었다. 결론적으로 악취 영향 범위는 악취 농도뿐 아니라 허용 빈도에 따라 변화하였다.

Matrix metalloproteinases (MMPs) have been known to affect to cell migration, proliferation, morphogenesis and apoptosis by degrading the extracellular matrix. In the previous studies, undifferentiated mouse embryonic stem cells (ESCs) were successfully proliferated inside the extracellular matrix (ECM) analog-conjugated three-dimensional (3D) poly ethylene glycol (PEG)-based hydrogel. However, there is no report about MMP secretion in ESCs, which makes it difficult to understand and explain how ESCs enlarge space and proliferate inside 3D PEG-based hydrogel constructed by crosslinkers containing MMP-specific cleavage peptide sequence. Therefore, we investigated what types of MMPs are released from undifferentiated ESCs and how extracellular signals derived from various niche conditions affect MMP expression of ESCs at the transcriptional level. Results showed that undifferentiated ESCs expressed specifically MMP2 and MMP3 mRNAs. Transcriptional up-regulation of MMP2 was caused by the 3D scaffold, and activation of integrin inside the 3D scaffold upregulated MMP2 mRNAs synergistically. Moreover, mouse embryonic fibroblasts (MEFs) on 2D matrix and 3D scaffold induced upregulation of MMP3 mRNAs, and activation of integrins through conjugation of extracellular matrix (ECM) analogs with 3D scaffold upregulated MMP3 mRNAs synergistically. These results suggest that successful proliferation of ESCs inside the 3D PEG-based hydrogel may be caused by increase of MMP2 and MMP3 expression resulting from 3D scaffold itself as well as activation of integrins inside the 3D PEG-based scaffold.

Since the advent of smart phones in 2007, the population of smart phone user has been increasing at stunning rate in Korea. In spite of many benefits such as full internet access, location based service, and easy installation of new applications, many social and medical researches have been reported the concern of overuse of smart phones, especially for teenagers on game and internet addiction. However, most of studies are based on the survey research, so often questioned to be limited number of sample and subjective, which can be overcome by evident-based study. The paper presents a pilot development of Google Android app that provides the 24-hour-measured application usage pattern of smart phone users. A requirement study performed with medical doctors and phycologists are discussed and the key technical and application issues are discussed and their solutions are presented. The system is designed to be extensible so that social network and additional service can be applied based on the user. The measured data is expected to be used for study the cause and treatment of game and internet addiction for teenagers and adults.

OSHMS(Occupational Safety and Health Management System) is able to define as a typical convergence of all kinds of activities and elements that support maintaining safety at workplace and protecting employee's health. In particular, fundamental function of OSHMS is preventing diverse hazards at workplace through high level of regulation compliance. Furthermore, insuring safety in workplace, protecting employee's health and increasing effectiveness in manufacture are OSHMS's additional functions. Based on the division of manufacture in 2009 Survey on Current Status of Occupational Health & Safety data, the study analyses certain relation between OSHMS and level of regulation compliance. The main finding is that most activities and elements which formed by OSHMS are being affected by regulation clauses.

OSHA(Occupational Safety and Health Act) generally regulates employer's business principles in the workplace to maintain safety environment. This act has the fundamental purpose to protect employee's safety and health in the workplace by reducing industrial accidents. Authors tried to investigate the correlation between 'occupational injuries and illnesses' and level of regulation compliance using Survey on Current Status of Occupational Safety & Health data by the various statistical methods, such as generalized regression analysis, logistic regression analysis and poison regression analysis in order to compare the results of those methods. The results have shown that the significant affecting compliance factors were different among those statistical methods. This means that specific interpretation should be considered based on each statistical method. In the future, relevant statistical technique will be developed considering the distribution type of occupational injuries.