본 연구는 식물성 불포화지방산 복합제로 이루어진 보조사료를 첨가하여 이유자돈의 균일성과 증체성적에 미치는 효과를 구명하기 위하여 수행하였다. 공시동물은 일반 종돈장의 Landrace 모돈 110두와 포유자돈 248두를 공시하였다. 시험구는 모돈시험에서는 장쇄지방산 복합제제를 분만사입식부터 이유시까지 약 4주간 급여하였고 자돈시험에서는 액상 중쇄지방산 complex를 포유기간 중 2회 구강투여 하였다. 모돈 시험에서 복당생존자돈수와 복당생시체중은 처리구간 차이를 보이지 않았으나, 복당이유자돈수와 복당이유체중에서는 시험구가 대조구에 비해 유의적으로 높았다(P<0.01). 모돈의 사료섭취량은 시험구가 유의적으로 높았으며, 발정재귀일령은 유의적으로 차이는 없었으나 시험구에서 평균 0.3일 정도 빠르게 나타났다. 포유기간내 일당증체량 역시 이유체중과 양의 상관관계를 갖고 있어 유의적인 차이가 있는 것으로 나타났다. 자돈시험 결과 시험구는 포유기간내 이유체중이 대조구에 비해 유의적으로 높았으며 생시체중 대비 증체량도 같은 기간에 많은 차이를 나타내었다. 자돈의 이유체중에 대한 변이의 지표를 보면 시험구와 대조구간 변이의 차이는 없는 것으로 나타나 개체간의 균일성에 대해서는 처리간차이는 나타나지 않았다. 결론적으로 포유기의 장쇄지방산 급여는 모돈의 젖에서 올레인산, 리놀산 등불포화지방산의 함량을 증가시키고, 젖 분비량과 사료섭취량을 증가시켜 자돈의 초기증체에 기여하는 것으로 나타났고, 자돈에 대한 중쇄지방산의 급여도 이유체중을 증가시키는 효과를 가져왔다.
Odors were produced in the reactor where contained the swine excreta sampled from the pigery and were analyzed the major odor materials based on the odor contribution index. The container hoggery where six pens were separated was set up in S-myun, Gyeonggi-do, Korea: one was control group, the other were experimental group. Sixty weaners were raised and their excreta were sampled every week to the test of the effect of feed type probiotics. Some probiotics showed very effective deodorizing activity during the whole experimental periods. All products had positive effects on the reducing odor intensities after 4 to 5 week periods. The odor contribution analysis represented the main odor compounds were trimethylamine and volatile sulfur compounds such as hydrogen sulfide, mercaptane, and dimethyl sulfide.
Although several enteric viral pathogens including the porcine groups A, B and C rotaviruses (PGARV, PGBRV, and PGCRV), sapovirus (PSaV), and torovirus (PToV) are known to cause endemic diarrheas in weaning and post-weaning piglets, their precise prevalence in Korea is not clear. Therefore, we examined 1,222 diarrhea stools obtained from 627 farms during 2004~2009 by RT-PCR and/or nested PCR for evaluating their precise prevalence in Korea. PGARV was the predominant pathogen during 2004~2007 but its prevalence was markedly reduced during 2008~2009. PGBRV infections caused endemic diarrhea during 2004~2007, but was hardly detected during 2008-2009. PGCRV was detected at 27.0%, 14.5%, 42.4%, 28.8%, 7.3%, and 54.2% during each year of 2004~2009, respectively, indicating its high prevalence in Korea throughout the years. PSaV induced with high prevalence (32.4-39.2%) during 2004~2005 but its detection rate was markedly decreased during 2006~2009. PToV caused sporadic infections only during 2006 (1.0%) and 2007 (6.9%). These enteric viruses were detected in diarrhea specimens in piglets usually in combination with each other and/or together with bacterial pathogens including the Escherichia coli, Salmonella spp., Brachispira hyodysenteriae, and Lawsonia intracellularis. Infections with PGARV, PGCRV, PSaV, and PToV were more prevalent in fecal samples collected in cold seasons. These results provide important epidemiological data for the control and establishment of a surveillance system for the prevailing enteric viruses in Korea.
Two experiments were conducted to determine the effect of wood vinegar (WV) on the fecal microbes and gas emission in weaned pigs. In Exp. 1, 224 weaned piglets (L×Y×D, 21 d-old, initial BW 6.02 ± 0.52 kg) were assigned to four treatments of different WV levels in randomized completely block design with four replicates (14 piglets/pen) for 28 days, including phases I (0-2 wk) and phase II (3-4 wk). The dietary treatments were 0, 0.1, 0.2 and 0.3% of WV added to a corn-soybean meal basal diet. The concentration of ammonia measured at 0 h and 24 h in feces did not differ among dietary treatments (p>0.05), but it was linearly decreased (p<0.05) in 0.2% and 0.3% addition level measured at 48 h and 72 h. The concentration of hydrogen sulfide had no significant change (p>0.05) within treatments measured at different time. The fecal total bacteria (TBC), Lactobacilli(LAB) and E. coli were measured on 14 d and 28 d. TBC were higher (p>0.05) in WV added treatments than the control. Similar results were obtained for LAB. But E. coli populations were decreased (p<0.05) in treatments added WV compared to control measured on 14 d and 35 d. In Exp. 2, 288 weaned piglets (L×Y×D, 21 d-old, initial BW 6.62 ± 0.31 kg) were assigned to four treatments in a randomized completely block design with four replicates (18 piglets/pen) for 28 days. The dietary treatments were basal diet (negative control, NC), 0.2% organic acid (positive control, PC), 0.2% antibiotic (AT) and 0.2% WV added to a corn-soybean meal basal diet. The fecal total bacteria (TBC), Lactobacilli(LAB) and E. coli were measured on 14 d and 28 d. TBC, LAB and E. coli showed lower counts in pigs fed AT diets than others. In conclusion, these results indicated that wood vinegar could reduce the NH3 concentration of feces and inhibited the growth of harmful bacteria.
This study was conducted to determine the replacement effect of spray dried plasma protein (SDPP) with dried porcine solubles (DPS) in weaning pigs. An ileal digestibility trial, at first, was conducted to formulate the experimental diets with SDPP or DPS. Six piglets (21-d old and 6.12kg BW) with T -cannula in the terminal ileum were used. In a feeding trial, 180 pigs (21-d old and 5.98kg BW) were alloted in a completely randomized block design. Treatments were T1 (SDPP 5%, DPS 0%), T2 (SDPP 5%, DPS 2.5%), T3 (SDPP 2.5%, DPS 0%), and T4 (SDPP 2.5%, DPS 2.5%) for phase I, and T1 (SDPP 3%, DPS 0%), T2 (SDPP 3%, DPS 1.5%), T3 (SDPP 1.5%, DPS 0%), and T4 (SDPP 1.5%, DPS 1.5%) for phase Ⅱ. Phase Ⅰ(0~1 week) diet was formulated to contain 3,300ME kcal/kg and 1.25% digestible lysine, and phase Ⅱ (2~3 week) diet contained 3,320ME kcal/kg and 1.10% digestible lysine. Chemical pompositions of the protein sources were higher in SDPP than DPS: CP (81.60 vs. 56.01), lysine (5.95 vs. 3.36), and methionine (1.85 vs. 1.22). Apparent ileal digestibilities of arginine, isoleucine, leucine, lysine, methionine, threonine and phenylalanine were higher in DPS than SDPP (p<0.05). The apparent ileal digestibility of essential amino acids (average) was also higher in DPS than SDPP (p<0.05). There were no significant differences (p>0.05) in ADG and ADFI of piglets during phase Ⅰ (0~1 week) and Ⅱ (2~3 week) among treatments. However, during phase Ⅱ, T2 showed better FCR than T1 (p<0.05). During the overall period, there was no significant difference in growth performance among treatments. When DPS was partially replaced for SDPP, the diet cost was significantly reduced (p<0.05) in terms of cost/kg of body weight. In summary, the digestibility of DPS was exellent, and it would be concluded that DPS can be partially replaced in the young pigs' diet containing SDPP in order to reduce diet cost in weaning pigs.
본 연구는 분만시 동복 신생자돈의 제대혈에서 추출한 genomic DNA를 PCR-RFLP 기법을 이용하여 농가수입증대를 위하여 육질이 불량한 PSS 돼지를 판별하는 방법을 개발하기 위한 기초실험으로써 그 결과를 요약하면 다음과 같다. 자돈 제대에서 혈액 genomic DNA를 추출하여 PCR에 의하여 증폭된 ryanodine receptor gene 영역의 산물은 자돈의 제대혈에서 1.8kb의 길이로 증폭되었음을 확인하였다. 제대혈에서 추출된 DNA 의 PCR 증폭 단편을 가지고 Hha I 제한효소로 digest 하여준 결과에서 PSS 돼지는 Yorkshire 종에서 출현하지 않았으나, Landrace 종과 Crossbred 종에서 각각 4.76%와 7.14%로 교잡종(LYD 또는 YLD)에서 더욱 많이 출현되었다. 이상의 결과로 볼 때 분만시 신생자돈의 제대혈을 채취하여 PSS 돼지를 조기에 선발하면 스트레스 감소는 물론 혈액채취의 간편성을 기대할 수 있을 것으로 판단된다.