This study investigated the antioxidant and antidiabetic activities of Stachys sieboldii Miq. extracts by solvents (water, ethanol, butanol, chloroform, and hexane). The contents of total polyphenols (7.18-37.25 mg/g) and flavonoids (0.21-5.21 mg/g) in extracts from Stachys sieboldii Miq. showed a significant difference dependent on the extraction solvents, butanol > ethanol > water > chloroform > hexane. Antioxidant activities by DPPH and ABTS radical scavenging were increased in a dose-dependent manner. These activity trends associated with the extraction solvent were different at each concentration, but resembled phenolic compound contents trend, generally. FRAP value increased in a dose-dependent manner, but there was a difference in radical scavenging activities when comparing between extraction solvents by butanol > ethanol > hexane > chloroform > water on all concentrations. The trend of α-amylase inhibition of extracts from 1,000 μg/mL to 2,000 μg/mL was not affected as enzyme activity is promoted and not inhibited. The inhibition of α-glucosidase was increased in a dose-dependent manner without water extracts, the activity on hexane extracts was higher than others per the extraction solvent. α-Glucosidase inhibition of hexane extracts showed 57.76% at 250 μg/mL, which is 2.8 times higher than the second highest chloroform extract (20.65%). From these results, we presume that the active ingredients of Stachys sieboldii Miq. is different according to the extraction solvent and also the activity is different by these major functional groups.

본 연구는 당뇨처방을 근거로 구성된 생약재 복합물의 항당뇨 효능을 조사하기 위하여 복합물의 인슐린성 물질을 탐색하고 -amylase 및 -glucosidase 저해활성을 평가하였다. 3T3-L1 세포에 복합 생약재 추출물을 의 농도로 처리하였을 때 지방세포로의 분화능은 151.7%로 인슐린의 작용을 향상시키는 것으로 확인되었다. 혈당저하제로서 생약재 복합 추출물의 -amylase에 대한 저해활성은 생약재 복합 추출물 10.0, 1.0 및 0.1

본 연구는 다양한 생약재를 사용하여 생약재 복합물을 구성하고, 복합물의 이화학적 특성과 항산화 활성에 대하여 조사하였다. 생약재 복합물의 추출 수율은 물 추출물이 35.00%, 에탄올 추출물 25.33%이었으며, 분획물 중에서는 ethyl acetate층의 수율이 물 및 80% 에탄올 추출물에서 각각 0.64% 및 3.76%였다. 생약재 복합물의 물 및 80% 에탄올 추출물의 총플라보노이드 함량은 각각 2.34% 및 2.42%이었고 총페놀성 화합