강원지방에 자생하는 야생의 초본식물 6종에 대한 실생번식법을 밝히고자, 1954년 봄과 가을에 종자를 채취하여 Gibberelin(GA3) 25 및 50ppm 수용액에 24시간 침지하였으며, 대조구로는 지하수에 24시간 침지시켰다. 처리된 종자를 1995년 4월 23일에 혼합토양(모래：peat moss： perlite： vermiculite, 1：1：1：1, v/v)을 채운 플라스틱 화분(직경 12,높이 11cm)에 40립씩 3반복으로 파종하여 2일에 1회씩 관수하면서 발아된 개체수를 조사하였다. 1995년 5월 13일에 채취된 할미꽃 종자는 1995년 5월 31일에 처리하고 6월 1일에 묘포에 파종하였다. 향유, 금낭화 및 배초향의 종자발아는 처리간 고도의 통계적 유의차가 인정되었으나 가는 쑥부쟁이, 거북꼬리 할미꽃 등의 종들에서는 처리간 유의차가 인정되지 않았다. 금낭화와 배초향에서는 25ppm, 향유에서는 50ppm 처리구에서 발아촉진 효과가 높은 것으로 나타났다. 각 야생초류의 자원화 가능성을 검토하였다.

금잔화, 들깨풀, 수국등 10종의 야생식물잎의 추출물이 담배나방(Heliothis assulta) 유충의 발육과 성충의 산란에 미치는 영향을 조사하였다. 그 결과 유충이 높았으며 대부분 1영과 2영 기간중 먹지않기 때문이다. 여기서 살아남은 유충들도 발육기간 및 탈피횟수는 증가하는 경향을 보였다. 이중 갈매나무와 때죽나무의 추출물은 성충의 산란도 상당히 억제하였다.

Chrysanthemums (Asteraceae) are important ornamental crops in worldwide that are well known as commercial valuable cultivars for cut flowers, potted plants, and garden flowering plants. Genus chrysanthemum consisted of 41 species that are mostly distributed in East Asia. Chrysanthemum has diverse ploidy levels with the basic chromosome number of x=9 from 2n=2x=18 (diploid) to 2n=10x=90 (decaploid). Fluorescence in situ hybridization (FISH) is a useful tool for studying the distribution of ribosomal DNAs. In this study, we have confirmed ploidy level by chromosome counting method. The somatic metaphase chromosome numbers were observed 2n=2x=18 in Chrysanthemum boreale, and 2n=6x=54 in C. indicum and C. zawadskii. More detailed Karyotype was constructed based on FISH method using 5S and 45S rDNA probes. Two (2) loci of 5S rDNA signals were detected in interstitial region of long arm chromosome in C. boreale and six (6) loci were in C. indicum and C. zawadskii. All of 45S rDNAs were located in terminal region of short arm chromosome which were visualize in six (6) loci in C. boreale and C. indicum and twelve(12) loci in C. zawadskii. In this study, it was the main topic to perform physical mapping of the location of 5S and 45S rDNA. Three of wild chrysanthemum showed variations in number of ribosomal DNAs. In the present investigation will help to further study of genome sequencing project in chrysanthemum.

Radish, Raphanus sativus L., is an annual vegetable of the family Cruciferae. Radish has RR genome with 18 somatic chromosome numbers (2n=2x=18). Until now, detailed karyotypic analysis is not only constructed only by conventional staining techniques but also other method. Fluorescence in situ hybridization (FISH) is a powerful molecular cytogenetic technique using chromosomal markers that reveal the positions of specific genes, such as ribosomal DNAs, thereby making it easy to identify individual chromosomes. We have constructed detailed karyotypes of four different local and wild varieties of radish, based on the chromosome arm length and fluorescence in situ hybridization (FISH) with the 45S rDNA and 5S rDNA as probes. As for the karyotype of radish, 9 pairs of chromosomes were extremely small in size with about 1 to 3 um in length at mitotic metaphase having metacentrics or submetacentrics. Three pairs of 45S rDNA signals and two pairs of 5S rDNA signals were observed in four radish species. One pair of 45S rDNA signal was located on terminal region of short arm chromosome, while two pairs were in interstitial region. Two pairs of 5S rDNA signals were located on interstitial region of chromosome. In conclusion, it was feasible to identify the radish by karyotype and physical mapping analyzed using ribosomal DNA.

포도 대목 품종 육성 시, 주요 교배친으로 사용되는 대목 품종 29점 및 국내 자생 머루를 포함한 포도 속(Vitis) 야생 유전자원 13점 등 총 42점의 유전적 다양성을 분석하기 위하여 RAPD와 SSR 분석을 수행하였다. Random decamer 30 종을 분석하여 329개의 다형성 밴드(60.3%)를 얻었으며 primer 당 평균 다형성 밴드 수는 11.0개였다. SSR 마커 20종을 이용하여 분석한 결과 263개의 대립인자가 확인되었고, 마커

There are about 16 species included in Fagopyrum (Polygonaceae) genus and some of them have been found recently and named. Fagopyrum genus is generally divided into two major groups: cymosum and urophyllum group. This study is to analyze the genetic diversity of Fagopyrum genus, to compare with the phylogeny result of Fagopyrim using previous analysis results, and to provide the information of each species specific marker by executing the cross-amplification on 3 species and 2 sub-species among cymosum group and 5 species among urophyllum using 136 SSR markers newly developed from common buckwheat. Cluster analysis using UPGMA showed two main clusters, each of the cymosum and urophyllum group. This result agree well with the previous findings on species relationships in Fagopyrum using different method approachs. In cross species amplification, our results revealed significant transferability of F. esculentum microsatellites to the 4 cymosum species (96.3% in F. esculentum ssp. Ancestral, 61.0% in F. tataricum ssp. tataricum, 36.0% in F. tataricum ssp. potanini, 97.1% in F. homotropicum). However, the percentage of 136 SSR markers were amplified in the urophyllum species (50.7% in F. urophyllum, 50% in F. lineare, 60.3% in F. leptopodum, 66.9% in F. capillatum, 66.2% in F. gracilipes) was lower than cymosum species, except F. tataricum. In addition, we identified 61 species specific markers among each species. These results demonstrate wide potential applicabilityof these markers for the study of inter-specific genetic diversity as well as evolutionary relationships among cultivated and wild buckwheat.

Several wild plant species are known to contain biologically active substances that are allelopathic to weed species as well as antioxidant to foods. Plant extracts or residues from leaves of 4 species, Achyranthes japonica (speedwell), Cucumis sativus (Cucumber), Trifolium repens (white clover), and Vicia angustifolia (narrowleaf vetch) were bioassayed against Medicago sativa (alfalfa) or Echinochloa crus-galli (barnyard grass) to determine their allelopathic effects, and used for measurement of antioxidant activities. The aqueous extracts applied on filter paper significantly inhibited root growth of alfalfa. Aqueous extracts or residues from V. angustifolia showed the most inhibitory effect on alfalfa or barnyard grass seedling growth and followed by A. japonica and T. repens. Oxidative stability by Rancimat method, antioxidant activity by TBA (2-thiobarbituric acid) method and DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity for the ground samples or methanol extracts were the greatest in V. angustifolia, although were less than those of commonly used antioxidants, BHT (butylated hydroxytoluene) and ascorbic acid. These results suggest that the wild plant species had potent allelopathic and antioxidant activities, and that their activities differed depending on plant species.