The entomopathogenic fungus Metarhizium anisopliae B is a powerful biological control agent against Monochamusalternatus, a crucial mediator of the pinewood nematode Bursaphelenchus xylophilus. In this study, production of destruxins(dtxs), insecticidal cyclic hexadepsipeptides, was monitored in the submerged culture of M. anisopliae B. Three typesof dtxs, i.e., destruxin A, B, and E, were produced during the culture. Among the three dtxs, the production yield ofdestruxin A was best, followed by destruxin B and E. Destruxin A production was increased when pH was controlledat 6.0, whereas production of destruxin E was not affected by the pH control. The highest yield of dtxs A, B, and Ewere 16.4, 7.3, and 6.1 mg L-1, respectively. Considering that process for dtxs production has not been optimized, M.anisopliae B has more powerful implication as a biocontrol agent.

Streptomyces species have been studied to find potent pest control agents as an alternatives to chemical insecticides.Previously, one of the ethyl acetate extract of Streptomyces isolates cultured on unpolished rice medium showed highlevel of juvenile hormone antagonist and larvicidal activities against pest insects including Aedes albopictus, Plutella xylostellaand Laodelphax striatellus. It has been known that the biosynthesis of secondary metabolites of Streptomyces could beinfluenced by a variety environments such as nutritional composition and growth conditions. In this study, to optimizeculture conditions for stable production of insecticidal compounds from this isolate, binding assay and bioassay-guidedmonitoring were conducted using various culture conditions.

Oyster mushrooms are one of the most famous mushrooms for foods in Korea. ‘Santari’ developed on this study is P. pulmonarius of oyster mushroom species. ‘Santari’ is a new variety of oyster mushroom for the bottle culture. It was bred by mating with monokaryons isolated from ‘GMPO20404’ and ‘Hosan’. The optimum temperature for the mycelial growth was 26～29°C on PDA medium and that for the primordia formation and the growth of fruiting body of ‘Santari’ was 22°C and 20°C on sawdust media. It took 34 days to finish spawn running, 3 days to finish primordia formation, 3 days to finish fruitbody growth in the bottle culture. In the characteristics of fruit body, pilei were round type and brownish, stipe color was white and stipe shape was long and thick. The yield per bottle was 172g/1,100ml and was 43% higher than that of control variety(Hosan). As results of the physical properties of fruit body, springness, cohesive, gumminess and brittleness of stipe tissue were 87%, 82%, 193g and 16kg, respectively. The values of them are lower than those of control except cohesive.

Oyster mushrooms are one of the most famous mushrooms for foods in Korea. ‘Santari’ developed on this study is P. pulmonarius of oyster mushroom species. ‘Santari’ is a new variety of oyster mushroom for the bottle culture. It was bred by mating with monokaryons isolated from ‘GMPO20404’ and ‘Hosan’. The optimum temperature for the mycelial growth was 26~29°C on PDA medium and that for the primordia formation and the growth of fruiting body of ‘Santari’ was 22°C and 20°C on sawdust media. It took 34 days to finish spawn running, 3 days to finish primordia formation, 3 days to finish fruitbody growth in the bottle culture. In the characteristics of fruit body, pilei were round type and brownish, stipe color was white and stipe shape was long and thick. The yield per bottle was 172g/1,100ml and was 43% higher than that of control variety (Hosan). As results of the physical properties of fruit body, springness, cohesive, gumminess and brittleness of stipe tissue were 87%, 82%, 193g and 16kg, respectively. The values of them are lower than those of control except cohesive.

Information and communication technology(ICT) around the world in the 21st century presents a new vision of agriculture. Time, place, and the high-tech industry to overcome barriers to the fusion of the so-called “smart agriculture” is changing the landscape of agriculture. Precision Agriculture’s core container production for the mushroom cultivation temperature, humidity, irradiation, self-regulation, such as carbon dioxide, the optimal environment for mushroom cultivation was implemented. The Lentinula edodes (shiitake) is an edible mushroom native to East Asia, which is cultivated and consumed in many Asian countries. It is considered a medicinal mushroom in some forms of traditional medicine. The fungus was first described scientifically as Agaricus edodes by Miles Joseph Berkeley in 1877. It was placed in the genus Lentinula by David Pegler in 1976. We controlled different light source (Blue-Red-White combined LED, blue LED, red LED and fluorescent light) with different intensity of LED irradiation (1.5, 10.5, 20.5 μmol/m2s for LEDs) to compare growth and development. Lights were treated with 12 hour on/ 12hour off cycle maintained in a controlled room with 19 ~ 21oC temperature, 80~90% humidity, and 1,000 ppm CO2 atmosphere concentration for 30 days. Growth and development differed from the LED color source and intensity of LED irradiation. Growth and development was most effective in 10.5 μmol/m2s for blue LED. All LED light sources showed less growth and development in lowest intensity of irradiation, which indicates that higher than 1.5 μmol/m2s for LED is not effective. After harvesting fruit bodies, we measured their weight and length, thickness of pileus and stipe, chromaticity, and hardness. 10.5 μmol/m2s blue LED group was the best result of harvest with average individual weight (24.7g) and length (61.98mm), thickness (29.93mm) of pileus and length (33.60mm), thickness (16.86mm) of stipe with fine chromaticity, hardness. This results show us that 10.5 μmol/m2s blue LED was the best effect on growth and development of Lentinus edodes (shiitake) mushroom’s ICT system container type environment.

Information and communication technology(ICT) around the world in the 21st century presents a new vision of agriculture. Time, place, and the high-tech industry to overcome barriers to the fusion of the so-called “smart agriculture” is changing the landscape of agriculture. Precision Agriculture’s core container production for the mushroom cultivation temperature, humidity, irradiation, self-regulation, such as carbon dioxide, the optimal environment for mushroom cultivation was implemented. Auricularia auricula-judae, known as the Jew's ear, wood ear, jelly ear or by a number of other common names, is a species of edible Auriculariales fungus found worldwide. The fruiting body is distinguished by its noticeably ear-like shape and brown colouration. The fungus can be found throughout the year in temperate regions worldwide, where it grows upon both dead and living wood. We controlled different light source (Blue-Red-White combined LED, blue LED, red LED and fluorescent light) with different intensity of LED irradiation (1.5, 10.5 and 20.5 μmol/m2s for LEDs) to compare growth and development. Lights were treated with 12 hour on/ 12hour off cycle maintained in a controlled room with 19 ~ 21oC temperature, 85~95% humidity, and 1000ppm CO2 atmosphere concentration for 30 days. Growth and development differed from the LED color source and intensity of LED irradiation. Growth and development was most effective in 1.5 μmol/m2s irradiation for blue LED. All LED light sources showed less growth and development in highest intensity of irradiation, which indicates that higher than 20.5 μmol/m2s for LED is not effective. After harvesting fruit bodies, we measured their weight, length, width, thickness and chromaticity of fruit bodies. 1.5 μmol/m2s blue LED group was the best result of harvest with average individual weight (5.38g), length (65.37mm), width (56.87mm) and thickness (13.51mm) with fine chromaticity. This results show us that 1.5 μmol/m2s blue LED was the best effect on growth and development of Auricularia auricula-judae mushroom’s ICT system container type environment.

In dieser Arbeit wurde versucht, die Perzeption der Studenten von dem Kulturlernen im Fremdsprachenunterricht(FU) zu erfassen und über Ausrichtung des Unterrichts für Kulturlernen inklusive Literacies zu diskutieren. Dabei ging ich davon aus, dass der Kulturunterricht an den koreanischen Hochschulen sogennate Multiliteracies einschließen sollen. Dazu habe ich Studenten befragt, um ihre Perzeption von Literacices beim Kulturunterricht zu erfassen. Davor habe ich ein paar Terminologie wie Literacies, Cultural Literacy, Intercultural Literacy, Critical Literacy, und Information Literacy neu definert. Nach der Analyse der Umfragenresultat habe ich die Aufsätze von den Unterrichtsführungen des Intercultural Literacy und Critical Literacy untersucht. Die Analyse hat es gezeigt, dass die meisten koreanischen Studenten Kulturelernen im FU als wichtig fanden. Dagegen glaubten die ähnlichen Zahl der Studenten, dass Critical Literacy im Kulturlernen weniger nötig als andere Literacies war. Das hängt von dem Schulsystem von Korea, weil Lernerinnen in der Schule Kritik nicht so einfach üben könnten. Daher soll man im Kulturlernen an den Hochschulen Critical Literacy behandeln. Außerdem soll man beim Kulturlernen Schwerpunkt an das Interculral Literacy legen, um Studentinnen bei der Vorbereitung auf die globale Welt zu helfen. Die Arbeit hat auf der Grundlage der vorherigen Untersuchungen darüber diskutiert, wie man Intercultural Litarcy und Critical Literacy ins Kulturlernen einführen kann.

Tissue engineering (TE) has been developed to create functional organs and tissue by combining 3D matrix and cells in vitro. Vascularization and angiogenesis are utmost important for supply of nutrients and oxygen in tissue engineered organs. The present study was performed to isolate and characterize primary endothelial cells (EC) from aorta of alpha 1, 3-enzyme galactosyltransferase knock out (GalT KO) pig, to minimize immune rejection and analyze body immune system for future xenotransplantation studies. Isolation of primary EC from aorta were performed by incubation with dispase for 8-10 min at 37°C. Primary EC were cultured in EC growth medium on different extra cellular matrix (ECM), either collagen or gelation. Primary EC exhibits morphological characteristics and showed positive expressions of EC specific marker proteins i.e. PECAM1, KDR and VWF despite of their ECM surface; however, on collagen based surface they showed increase in mRNA level analyzed by qPCR. Primary EC cultured on collagen were sorted by flow cytometer using KDR marker and cultured as KDR positive cells and KDR negative cells, respectively. KDR positive cells showed dramatically increased in PECAM1 and VWF level as compared to KDR negative cells. Based on the above results, primary EC derived from GalT KO are successfully isolated and survived continuously in culture without becoming overgrown by fibroblast. Therefore, they can be utilize for xeno organ transfer, tissue engineering, and immune rejection study in future.

반하(Pinellia ternata(Thunb.) Breit)는 천남성과에 속하는 다년생 초본식물로서 조직배양을 이용하 여 대량번식 방법 연구가 활발히 진행되어 왔다. 하지만 대량생산된 괴경들의 토양 순화 및 적응을 위 한 환경 조건의 확립의 연구는 미비하다. 따라서 본 연구에서는 기내에서 증식된 반하의 괴경을 이용하 여 우수한 품질의 약재와 건전묘의 대량생산을 위해 생육에 적합한 토양조건을 탐색하였다. 본 연구에 서는 액체배지에서 현탁배양 한 괴경(Type 1)과 고체배지에 치상하여 배양된 괴경(Type 2)두 종류를 비 교하였다. 토양은 3개의 조성으로 조합하여 생육을 비교하였으며, 코코피트, 피트모스, 버미큘라이트, 펄라이트 및 제오라이트를 배합하여 사용하였다. 기내 배양 조건이 다른 처리구들의 토양 조성별 순화 율 측정하였으며, 생육의 차이를 확인하기 위해 8주동안 생육 후 초장, 잎 수, 마른잎 수, 괴경 수, 괴 경 크기, 생체중 및 건물중을 측정하였다. 또한 주아의 생성율을 확인하기 위하여 4주와 8주에 측정을 진행하였다. 그 결과 Type 2가 펄라이트를 20%증량한 상토 B에서 가장 우수한 생육을 보였다. 괴경의 비대에 영향을 주고 묘로서 이용을 위하여 필요한 잎의 수는 상토 B에서 가장 많은 1.7개로 나타났고 가장 잎의 출현이 없었던 Type 1의 상토 C가 0.9개로 나타나 1.8배의 차이를 나타냈다. 또한 같은 처 리구에서 건물중이 통계적으로 유의한 차이를 보이며 우수한 것으로 나타났다. 반하의 주아 생성율의 차이는 상토 B에서 1.1개로 우수하였으며, 가장 저조한 처리구의 수치보다 1.2배 높은 생성율을 확인할 수 있었다. 이러한 결과로 기내에서 배양된 반하의 토양 순화 및 적응기에 괴경의 비대를 유도하여 우 수한 한약재 생산이 가능 할 것으로 생각되며 차후 토양에서 재배 시 반하의 대량번식에도 도움이 될 것이라 사료된다.

It is certainly not possible to analyse the evolution of the global luxury consumers orientations for the new luxury Chinese brands without considering the essence and the impact of the “brandscape”. In the last decade, China has assisted to the surge of the “luxury lifestyle” for a multiplicity of consumer segments living in those coastal areas – and not only - filled with luxury and fashion brands, that invaded every city area from streets to airports from clinics to hotels where concept stores, luxury flagship stores, sponsorships for events and urban artefacts “add value to the symbolic production of an urban lived space” (Bellini and Pasquinelli, 2015). Luxury product brands are enriched by the synergy with the city brand and the diverse fashion and art city locations, activities and events. In the new luxury perspective that sees luxury in its experiential dimension and no longer only in desire of an exclusive object, the relation of luxury brands and city brand requires a specific focus, in particular in the new fast growing economies as China that sees the rise of the new experiential luxury lifestyle and new local luxury brands. In the fast growing luxury Chinese luxury market where new Chinese luxury brands are striving to acquire a brand identity and image first in the local market and then in the international one, city branding may be a conductive solutions for brand value and identity creation. Authentic luxury experiences in significant city contexts appear added value activities for luxury brands in particular for those with no consolidated heritage and identity as the new Chinese luxury brands. New retail formats such as pop-up stores, concept stores located in specific high value artistic or fashion related locations adds value (Bellaiche et al, 2012). For Chinese luxury brands with a very limited identity, a almost absent heritage and a ongoing value creation of the brand, in-store experience is increasingly important (Atsmon et al, 2012) and the shopping location certainly represent an important factor for the increasingly diverse and demanding luxury customers by being not only the instrument towards the desired subjects but also a value-adding experience on its own (Rintamaki et al, 2007, p. 628). The emergence of the Chinese luxury consumer did not mean the presence on a market where the consumers are gathered by the same tastes, desires and purchasing patterns. Reference to the global consumer culture and paradigm evidenced that consumers in diverse geographical contexts may have different and sometimes even conflicting opinions or shared desires and values expressed in similar behaviours or symbols towards a brand. Global brands sets the international standards and convey shared symbols (Holt, Quelch and Taylor 2004) and a myth of cosmopolitanism to which many consumers world-wide appreciate (Strizhacova, Coulter and Price 2008).Brands represent a form of culture and they relate to the way people live, think, eat and choose to wear as well, a form of seeing life and the world (Askegaard, Kjeldgaard and Arnould, 2009) . Luxury brands have become increasingly present in the Chinese consumer market and lifestyle and the role of purchasing luxury goods experiencing a luxury lifestyle has taken an unexpected importance and meaning in the Chinese social context. China has started to experience the consumer culture only after China's opening up to the market economy as a result of the economic reforms post-1979 that have given to "aspirational" consumers more freedom to develop a consumer culture partially away from political limitations but still permeated in the Chinese culture and its characteristics. Those reforms have also given rise to the private businesses and the birth of a consumer middle class, "the new rich", in China. The birth of the Chinese middle class has fuelled the emergence of a highly diversified consumer class with different purchasing attitudes (Latham, 2006) and a new way to express their taste, their motivation for purchasing (Gillette, 2000) and in particular an increasing brand awareness, mode of purchasing and conceptualisation of luxury (Rambourg, 2014; Rovai, 2016). Distinctive aspects of luxury consumer culture have started to emerge in the late years, evidencing new desires for Chinese luxury consumers with respect to luxury brands, accompanied by the entrance in the market of Chinese luxury brands aspiring the capitalise on the increasing "Chinese luxury desire" but limited by their lack of specific characteristics of authentic luxury brands - heritage, identity and prestige amongst others. As a result, this research focuses on the analysis of Chinese luxury brands presence in the local Chinese urban context; specifically, it focuses on how the Chinese urban fashion context can help to support the creation of a luxury brand value and also reinforce a luxury brand identity and image in a Chinese luxury consumer culture that does not possess a luxury heritage. An analysis of two luxury Chinese brands and a local luxury and fashion concept store has been initiated together with further evidence from the Shanghai urban context, its activities, events and cultural specifics together with the following a qualitative method and in particular Yin (1989) case study approach. A series of 15 interviews have been held in late 2016 in Shanghai with the two Chinese luxury brands creative designers, owners and staff during one month together with observation and consulting of documents. Literature review has focused on the role of individual brands that, being somehow associated with the city become a collective brand (Pasquinelli, 2014), framing "the complex network of associations, linking products, spaces, organizations and people (Bellini and Pasquinelli, 2015). Initially, an important attention has been oriented towards the geographical associations to the country-of-origin effect (Bilkey and Nes, 1982; Johansson et al, 1985) later on evidencing that a defragmentation into of smaller geographical units may be appropriate at urban level (Bellini and Pasquinelli, 2015) to highlight the relevance of the "origin" not simply in relation to a broad geographical context where the brand manufactures a product but also „the place, region or country where a brand is perceived to belong‟ (Thakor and Kohli, 1996, p. 26). The origin being not only a matter of product production but more of product conceptualisation, perception or consumption going towards the "brand product usage context" (Gerr et al, 1999). Brand product usage happen in those spatial circuits whose cities are part of and whose role may be conductive to the „local origination‟ of product brands, adding value to the birth and internationalisation of locally originated brands (Pike, 2011). Those local brands are developed from an ecosystem composed by relations and ownerships involving a multiplicity of stakeholders whose customers are an integral part (Power and Hauge, 2008). In the literature, Fashion capitals is a unique case of those ecosystems with a specific relationship between industry and spacial circuits is based on the urban context instrumental to fashion creation and also to consumption (Breward and Gilbert, 2006). The city as a part of the consumer culture and in particular as part of the brand product experience (Thrift, 2004). As a result of the literature review and the conceptualisation of fashion capitals as ecosystems conductive to the fashion creation and consumption, an exploratory study of: Which context related variables affect new Chinese luxury brands identity and value and how the China fashion capital ecosystem affects Chinese luxury consumers brand perception. The paper will show an insight of the instrumental relation of the "brandscape" Shanghai and the impact on the Chinese luxury brands value and identity acquisition with respect to Chinese consumers.

Social media play a role in the value that sneakerheads, individuals who collect, wear, and trade sneakers with extraordinary effort and resources, place on specific brands and models of sneakers. In-depth interviews with 25 sneakerheads, recruited through sneakerhead online communities and snow-ball sampling, were conducted to explore how social media contribute to emotional and monetary value creation of sneakers, as well as how social media is used across the Inventory Ownership Cycle (IOC) which includes pre-acquisition, acquisition, physical possession, and disposal of sneakers (Boyd & Mcconocha, 1996). Finding shows that during the pre-acquisition stage, emotional and monetary value is constructed when brands post images of celebrities wearing new models on various social media sites. Hype initially created by the brand is perpetuated by posts and conversations among sneakersheads. Perceived higher levels of hype lead one to believe demand surpasses supply, and therefore, monetary values of specific models increase. Through social media, consumers research and gauge the value not only of soon to be released sneakers, but also previously released sneakers only available on the resale market. During the acquisition stage, the online environment has become increasingly important. To avoid issues associated with selling high-demand/low-supply sneakers in- store, such as violent incidents and dishonest employees, many retailers have shifted sales of such product to online formats (Dunne, 2014). Social media serves as a platform for sneakerheads to learn about dates and locations of new releases and to locate resellers from whom they can purchase previously released sneakers. During the physical possession stage, sneakerheads engage with community members via social media; they show off their sneaker collections, as well as to obtain and share information regarding sneaker customization and maintenance to preserve aesthetics and value. It is through the engagement with fellow sneakerheads and the sneakers within their personal collections that emotional value can be further constructed. During the disposal stage, social media is used by resellers to determine monetary value, identify and communicate with potential buyers across the nation, and complete resale transactions. In summary, this research demonstrates how sneakerheads use social media throughout the IOC, as well as the role social media plays in the construction and identification of sneaker value.

Unlike older generation whose consumption of music was bounded by their local culture, today’s young consumers access music beyond cultural boundaries. Many successful pop music in cyber space attracted billions of listeners from all over the world. The young generation born and raised in the digital age, are often thought to have altered sensory-neural characteristics because of their extensive use of electronic device since early childhood. This study investigates a perceptual saturation hypothesis which posits that in order to capture the young generation’s hard-to-get attention, online music must present a high level of energy and rhythm that is near the point of perceptual saturation. We conducted a functional near-infrared spectroscopy (fNIRS) experiment with sixty-four young adults and found that total cerebral blood volume over prefrontal brain area was higher for a song that presents acoustic intensity near the point of perceptual saturation than a counterpart stimulus with lower levels of acoustic intensity. The degrees of prefrontal hemodynamic randomness decreased significantly while the participants listened to YouTube music that provided high levels of acoustic stimulation. Online popularity, recorded as the number of daily hits, was positively correlated with the total cerebral blood volume and negatively correlated with hemodynamic randomness.

Study on Hallyu contents consumption habits according to religion was not covered as a research topic in a variety of Hallyu studies, but the acceptance of Hallyu content, of course, is a very important variable affecting the overall distribution and consumption. Therefore this study focused on literature and expert FGI in relation with the three major religions Buddhism, Islam, Catholicism and an survey research was held with Buddhism, Islam, Catholic countries such as Vietnam, Malaysia, Costa Rica, Peru, Chile. Based on this analysis, the results are discussed. As a result of analyzing the answers of the experts participated in the FGI, 3 emerging issues and 18 sub-issues were identified. 3 main issues are the diffusion state of Hayllu content, the problems in the diffusion process of Hallyu content, and the diffusion method of Hallyu content. Expert reported Buddhist countries in and around Southeast Asia is on a maturity phase and Muslim regions focused on Turkey and the Middle East is on a growth phase. In addition, Catholic region with a focus on Latin America had been understood that the introductory period has passed but somehow entered the lower level of growth than the Islamic sphere. Specially, it was confirmed by the expert interviews that our Hallyu content was mainly spread in the Buddhist countries in and around Southeast Asian region. The summary of the main results of the survey in Buddhist and Islamic countries people who experienced Hallyu content, ‘drama’ had the highest response and in Catholic countries, ‘K-Pop’ had the highest response. Secondly, the experience regarding Korean culture has shown to be ‘Korea food’ regardless of the religious sphere. The contacting channels of Hallyu content were through the free internet services such as youtube, P2P and etc. regardless of the religious sphere. Genre preference in Buddhist countries chose ‘Entertainment’, Islamic countries ‘drama’, Catholic countries ‘K-Pop’ as their favorites. The most preferred Korean drama genre showed to be ‘melodrama’ regardless of the religious sphere and ‘limited amount of contents’, ‘inconvenient content transfer process’ showed to be the highest response to the inconvenience using the contents. Results of examining the impact of the contents on religious image of Korea, it is recognized as a positive impact regardless of the religious sphere. Results of examining the impact of Hallyu content on religious life, neutral response such as "it has nothing to do with religion" was the highest. This shows the Korean Culture & Content does not help, nor disturb the religious life. Interest in the Korea Culture and the craze in the popular culture throughout China, Japan, South East Asia, and the spread of Korean drama, movies, and K-Pop through Europe and South America is cultural phenomenon that is increasing. Based on this, by covering areas such as fine arts, celebrity, tourism and etc. it is expanding gradually from culture Hallyu to economy Hallyu. However, focusing on the short term economic performance on the traditional Hallyu can’t avoid the criticism. It will be able to provide new country image and brand management focusing also on social, cultural and religious values besides the economic benefits. In particular, by analyzing the consumption based on the religion it is possible to derive sustainable growth and development of Hallyu as a cultural content, and in terms of culture communication Hallyu consumer analysis and response as strategic, politic planning. Through this study, if an in-depth study of strategic subject and action plans research is followed, policy utilization value can be even greater.

Drawing on data collected through archival research, semi-structured interviews, and site observation, this study examines the conditions underlying the emergence of craft beer culture in Finland. In doing so, the study seeks to shed light on phenomena underpinning the emergence of global consumer culture in unfavorable environments. A hermeneutic analysis reveals three themes that contribute to the emergence of craft beer culture in Finland: craft brewers as Davids vs. Goliath, craft beer consumption as consumers’ identity work, and bartenders and brewers as cultural intermediaries. On a broad level, the study contributes to literature examining the impact of globalization on consumer behavior by illustrating how global consumer culture presents local consumers a means to resist dominant local cultural and market structures. The study also extends research on consumer acculturation to global consumer culture by highlighting the importance of cultural intermediaries in the acculturation process. Implications of the study include the finding that consumer resistance towards local hegemonic consumptionscapes presents an entry point for global consumer culture, allowing international marketers to tap into local consumers’ desire to defy and resist local cultural and market structures deemed as restrictive or oppressive. The study also argues that international marketers are wise to consider the influence of cultural intermediaries in introducing and disseminating new consumption practices, especially in the case of products at the forefront of new global consumption trends.

There has been an emerging interest in the effective luxury advertising, which has been conducted within and across national borders. Unlike earlier studies on luxury brands that focused on the behavior and opinions of luxury consumers (e.g., luxury motivations, value perceptions, etc.), this nascent stream of research queries an important role that advertising exerts on luxury consumers (Freire, 2014). Informed by these developments, our study examines how luxury brand marketers can design effective social media messages for their consumers. In particular, we draw on recent research in consumer psychology to shed new light on (1) how consumer feelings about the psychological distance of luxury consumption may influence their evaluation of different types of message appeals on social media and their intention to share these messages with others; and (2) we address how this process varies depending on (a) the perceived tie strength between consumers on social media, the functional attitudes of luxury brands, and across different cultural milieus.

This research analyzedconsumer culture and usage of sugar in modern times based on 12 modern popular Korean cooking books with sugar recipes. Procedures were formed via textual analysis. The outcomes of the study can be summarized in brief statements. According to「Banchandeungsok」,「Booinpilj」, and「Chosunmoossangsinsikyorijebeob」, sugar was utilized in 34 out of 663 or 5.1% of cooked foods during the 1910s to 1920s. According to books such as 「Ganpyounchosunyorijebeob」,「Ililhwalyongsinyoungyangyoribeob」,「Chosun’s cooking of the four seasons」,「Halpaengyoungoo」, 「Chosunyorijebeob」, and 「Required reading for housewife」, sugar was added to 165 out of 998 or 16.5% of cooked foods during the 1930s. According to the books like「Chosunyorihak」,「Chosunyoribeob」, and「Woorieumsik」, sugar was an ingredient in 241 out of 756 or 31.9% of cooked foods during the 1940s. Sugar depicted within the 12 modern popular Korean cooking books primarily functioned as an alternative sweetener, starch, sweet enhancer, preservative, and seasoning. Similar to illustrated sugar from modern popular Korean cooking books, sugar has continually been favored by Korean cooks starting from the 1910s with 5.1% usage, the 1930s with 16.5% usage, and the 1940s with 31.9% usage. Despite its short history, sugar’s culinary importance in Korea has been on the rise ever since the early 1900s. Although sugar is an exotic spice in Korea, it has gained social, cultural, and symbolic recognition as well as practicality within Korean food culture. Thus, it has become more internalized and familiarized as an inseparable sweetness that characterizes current Korean food.

본 연구는 청소년 온라인 게임문화가 어떻게 청소년의 일상문화로 자리 잡았으며 여가의 주요 수단으로 부상하였는지에 대한 시기적 구분과 아울러 확장 배경에 대한 사회구조적 접근을 시도해 보았다. 한국의 온라인 게임은 ‘상호작용성’을 기반으로 1998년 1차 파동과 2005년 2차 파동을 통해 청소년 일상 문화 전반의 변형을 가져왔다. 분명한 것은 온라인 게임 공간 내에는 청소년들의 지속적인 사회적 관계가 이어지고 있다는 것이다. 본 연구의 결과는 청소년 온라인게임 문화 및 청소년 일상문화 연구에 있어 균형적인 연구의 촉매제 역할을 함과 동시에 다양한 하위문화별 연구의 기초 자료로 활용될 수 있을 것이 다. 또한 본 연구에서 제시한 상호작용성을 기준으로 한 1,2차 파동에 대한 여러 비판적 후속 연구의 가 능성은 온라인 게임으로 인해 변화한 청소년 일상문화 연구의 학술적 공백들을 매우는 발전적 과정이 될 것이다.

Lysophosphatidic acid (LPA) is an important signaling molecule which mediates many different cellular responses. The purpose of this study was to investigate the effect of in vitro culture (IVC) medium supplemented with LPA on the preimplantation embryonic development of porcine embryos derived from in vitro fertilization (IVF). Embryos derived from IVF were cultured in PZM-3 medium supplemented with 30 μM LPA on Day 1 to Day 7, Day 1 to Day 3 (early stage), or Day 4 to Day 7 (late stage), or without LPA. Moreover, the messenger RNA (mRNA) expression of obtaining blastocysts from each group were analyzed. Data were analyzed by ANOVA followed by Duncan using SPSS (Statistical Package for Social Science) mean ± SEM. There was a significantly higher cleavage rate in Day 1 to Day 7 than control (71.25% and 57.46%, respectively) and significantly higher total cell number of blastocysts in Day 1 to Day 3 and Day 4 to Day 7 than control (56.07,56.53 and 45.19, respectively). The results also showed that the mRNA expression level of PCNA, Bcl-2 and Bax in Day 1 to Day 7 group blastocysts were significantly higher than control and the expression level of Bax in Day 1 to Day 3 was also significantly higher than control. Moreover, it also showed that Bcl-2/Bax mRNA ratio in D1-3 group was significantly lower than control but D4-7 and D1-7 groups were comparable to control group. In conclusion, our results suggest that treatment with 30 μM LPA during IVC improves the porcine early embryo cleavage and the blastocyst total cell number after IVF and regulating the mRNA expression of blastocysts during blastocyst formation.

The nature of molecular mechanisms governing embryo development is largely unknown, but recent reports have demonstrated that proper execution of programmed cell death is crucial for this process. The main objective of this study is to examine the mode of programmed cell death during nuclear transfer embryos development in porcine. In particular, the relative employment of two major pathways in programmed cell death; e.g. apoptosis (type I) and autophagy (type II) was compared. Oocytes use in the study was matured in vitro in the presence of 10% FBS maturation medium. After nuclear transfer embryos were cultured for each programmed cell death control factor [Cysteamine(Cyst : 0.4mM), 3-methyladenine(3MA : 2.5mM) and Rapamycin(RP : 100nM)] in TCM-199 medium supplemented with 0.1% BSA. In this study results of among the blastocysts development in 3MA; PCNA, MAP1LC3A and ATG5 RNA gene expression level increased in the order of IVF<Cyst < 3MA < RP. However Casp-3 and TNF-r RNA gene expression level decreased in the order of IVF < 3MA and RP< Cyst. The expression of mTOR showed a pattern opposite to that of MAP1LC3A. That is, its expression was the lowest in Cyst group. And next experiments analysis of MMP expression patterns. Analysed this MMPs enzyme activation to evaluate the effectiveness of high quality brastocyst culture in porcine. In this results of the enzymatic activity of MMP-2 and MMP-9 was assessed in culture, the level of active MMP-9 was higher expression in the medium of each 3MA and RP treatment group, with the level of another treatment group being relatively higher. These results suggest that the autophagy activation culture medium is more effective for stable and innovative nuclear transfer embryos development.