넙치 치어에 대한 나프탈렌의 급성독성을 조사하기 위하여 대조구(0μg/L), 1000, 1800, 3200, 5600, 10000μg/L의 6개의 나프탈렌 농도구를 설정하여 24시간 동안 노출실험을 실시하고 혈액학적 성상을 분석하였다. 넙치 치어에 대한 나프탈렌의 24h-LC50은 3600μg/L를 나타냈다. 넙치 치어의 핼액학적 반응에서 헤마토크리트값은 5600, 10000μg/L의 농도구에서 대조구와 비교하여 유의하게 증가하였고, 글루코스는 10000μg/L의 농도 구에서 유의하게 증가 하였다(P〈0.05). 삼투질 농도는 3200, 5600, 10000μg/L의 농도구에서 유의하게 증가하였다. 반면, 이온분석 결과 [Na+], [K+]은 5600 및 10000μg/L 나프탈렌 농도구에서 유의하게 증가하였으나 [Cl-]는 큰 차이를 보이지 않았다.

A field study was conducted to compare growth, feed utilization and nutrient excretions by feeding extruded pellet (EP) and moist pellet (MP). Slow growing flounder were used as the experimental animal to find out a prominent result in terms of growth and feed utilization. Weight gain of fish fed the experimental diets for 8 weeks reached 105.3 g for EP and 107.1 g for MP, while fresh feed intake was 98.7 g for EP and 279.4 g for MP. Feed conversion ratio was 0.94 and 2.61 for EP and MP, respectively which was 0.77 and 0.94 when corrected on dry matter basis. Even though protein efficiency ratio (2.26 vs 2.21), specific growth rate (0.69% vs 0.70%), daily weight gain (2.06 g vs 2.10 g) and mortality (4.0% vs 4.1%) were similar between EP and MP, protein retention efficiency of fish fed EP was superior (53.3%) to that of fish fed MP (39.6%). Protein intake of fish for 8 weeks was similar from 52.3 g for EP to 53.3 g for MP, while protein gain was higher in fish fed EP (27.9 g) than in fish fed MP (21.1 g). Protein excretion by feeding EP and MP reached 232 g and 300 g, respectively per kg gain, suggesting that protein loads could be greatly reduced in practical culture system through the use of EP. Phosphorus (P) intake was much lower in fish fed EP (1.63 g) than in fish fed MP (2.18 g), while retention of P was higher in fish fed EP (0.88 g) than in fish fed MP (0.78 g), showing the retention efficiency of 54.0% and 35.9%, respectively. Fish fed EP excreted 7.1 g of P per kg gain which was much lower than that of fish fed MP (13.1 g). The present results clearly showed that EP feeding for flounder is not only productive but also environmentally friendly.

30일 동안 15 psu에서 순화 사육한 양식 넙치(평균전장: 20.8cm,평균체중: 96.1g)를 사용하여 염분변화에 따른 혈액 학적 변화 (cortisol, glucose, Na+, Cl-, K+, AST, ALT, hematocrit, RBC, hemoglobin) 및 생존율을 조사하였다. 사육수를 실험개시시 15 psu로부터 0 psu(group I, G I)와 35 psu(group II, G II)로 30분 이내에 교환하였다.

The response of hepatic mixed function oxygenase (MFO) system was investigated in olive flounder exposed to formalin. Hepatic microsome of olive flounder incubated in vitro with formalin demonstrated the induction of cytochrome P450 (CYP), ethoxyresorufin

사육수의 담수화시 수용밀도에 대한 생리학적 반응을 비교하고자, 30마리와 60마리씩 실험수조에 수용하여 30분이내에 사육수를 담수로 교환하였다. 혈장의 Cortisol 농도는 실험개시시 30SW에서 2.8 ng mL-1,60SW는 2.7 ng mL-1로부터 30FW와 60FW는 24시간째 각각 66.9 ng mL-1, 314.1 ng mL-1로 유의하게 높아졌다. 혈장의 Glucose농도는 60SW는 개